JPWO2014017642A1 - Biological wastewater treatment apparatus and biological wastewater treatment method - Google Patents

Abstract

The present invention comprises a signal substance production part that produces a signal substance by a signal substance producing bacterium and a treatment part that performs biological treatment of wastewater by the treated bacteria, and the signal substance production part sends the signal substance to the treated bacteria. A biological wastewater treatment apparatus having a supply means for supplying is provided.

Description

  The present invention relates to a biological wastewater treatment apparatus and a biological wastewater treatment method.

  It is known that microorganisms transmit information through intercellular signal transmitters (hereinafter referred to as “signal substances”) and control the secretion of pathogenic substances and biofilm production depending on the density of the microorganisms. It has been. Such an information transmission mechanism is called quorum sensing (see, for example, Non-Patent Document 1).

Current Opinion in Biotechnology, 2004, 15, 495-502.

  The present inventors have found a problem that the content of the signal substance is rapidly reduced when the signal substance is stored in water. Therefore, an object of the present invention is to provide a biological wastewater treatment apparatus and a biological wastewater treatment method that use a signal substance easily and efficiently to enhance the efficiency of wastewater treatment.

  The present invention comprises a signal substance production unit that produces a signal substance with a signal substance producing bacterium, and a treatment part that performs biological treatment of wastewater with a treated bacterium, and the signal substance production unit provides the treatment bacterium with the above-mentioned treatment substance. A biological wastewater treatment apparatus having a supply means for supplying a signal substance is provided.

  According to the biological wastewater treatment apparatus, the signal substance produced in the signal substance production section can be quickly supplied to the treated bacteria. Therefore, the signal substance can be used efficiently, and biological treatment of waste water by the treated bacteria can be promoted. On the other hand, when the signal substance is produced in a different place from the biological wastewater treatment equipment and added to the biological wastewater treatment equipment, the signal substance is less stable in water, so a sufficient effect can be obtained. It is not advantageous in terms of cost because there is no need to produce a large amount of signal substance in consideration of a decrease in the content.

  The signal substance production unit may exist outside the processing unit or may exist inside the processing unit.

  Further, the signal substance-producing bacterium may be in the form of floating cells or in a supported form.

  The signal substance-producing bacterium may be Paracoccus AS6 strain or Paracoccus AS13 strain. The Paracoccus AS6 strain was deposited on May 18, 2012 at the Japan Institute for Product Evaluation Technology Patent Microorganism Depositary Center (Kazusa-Kamazu 2-chome, 5-8-8 (Zip 292-0818), Chiba Prefecture). The strain with the accession number NITE P-1363 was established. No. 8 (postal code 292-0818)), and the accession number is NITE P-1364.

  The present invention further includes a signal substance production unit that produces a signal substance using a signal substance-producing bacterium, and a treatment unit that performs biological treatment of wastewater using the treated bacterium, and the signal substance production unit includes the treatment bacterium. A biological wastewater treatment method using a biological wastewater treatment apparatus having a supply means for supplying the signal substance to the step, wherein the signal substance producing section produces the signal substance in the signal substance production section And a step of supplying the signal substance to the treatment bacterium by the supply means, and a step of biologically treating the wastewater by the treatment bacterium in the presence of the signal substance in the treatment unit. A biological wastewater treatment method is provided.

  In the biological wastewater treatment method, the signal substance production unit of the biological wastewater treatment apparatus to be used may exist outside the treatment unit or may exist inside the treatment unit.

  The signal substance-producing bacterium may be in the form of floating cells or in a supported form. Further, the signal substance-producing bacterium may be Paracoccus AS6 strain or Paracoccus AS13 strain.

  It is preferable that the biological wastewater treatment method further includes a step of supplementing the signal substance producing part with the signal substance producing bacteria.

  Since the supply amount of the signal substance is stabilized by including the step of replenishing the signal substance producing bacteria, the biological treatment of the waste water by the treated bacteria can be further promoted.

  According to the present invention, it is possible to provide a biological wastewater treatment apparatus in which a signal substance can be used easily and efficiently and the efficiency of wastewater treatment is enhanced. Moreover, the biological waste water treatment method using the said biological waste water treatment apparatus can be provided.

(A) It is a schematic diagram which shows the biological waste water treatment apparatus used for the aerobic treatment which concerns on one Embodiment. (B) It is a schematic diagram which shows the biological waste water treatment equipment further provided with the final sedimentation basin 150 and the piping L160 used for the aerobic treatment which concerns on one Embodiment. (A) It is a schematic diagram which shows the biological waste water treatment apparatus used for the aerobic treatment which concerns on one Embodiment. (B) It is a schematic diagram which shows the biological waste water treatment equipment further provided with the final sedimentation basin 250 and the piping L260 used for the aerobic treatment which concerns on one Embodiment. (A) It is a schematic diagram which shows the biological waste water treatment apparatus used for the integral immersion type membrane-separation activated sludge method. (B) It is a schematic diagram which shows the biological waste water treatment apparatus used for a tank separation immersion type membrane separation activated sludge method. (C) It is a schematic diagram which shows the biological waste water treatment apparatus used for the membrane separation activated sludge method of a tank outer type. (A) It is a schematic diagram which shows the biological waste water treatment apparatus used for the integral immersion type membrane-separation activated sludge method. (B) It is a schematic diagram which shows the biological waste water treatment apparatus used for a tank separation immersion type membrane separation activated sludge method. (C) It is a schematic diagram which shows the biological waste water treatment apparatus used for the membrane separation activated sludge method of a tank outer type. (A) It is a schematic diagram which shows a biological waste water treatment equipment provided with a signal substance production part and piping for introducing a signal substance into a process part. (B) It is a schematic diagram which shows the biological waste water treatment apparatus in which a signal substance production part exists in the inside of a process part. It is a schematic diagram which shows the biological waste water treatment apparatus used for the nitrogen treatment which concerns on one Embodiment. It is a schematic diagram of the biological waste water treatment apparatus used for the nitrogen treatment which concerns on one Embodiment. It is a schematic diagram which shows the biological waste water treatment apparatus used for the nitrogen treatment which concerns on one Embodiment. It is a schematic diagram which shows the biological waste water treatment apparatus used for the phosphorus treatment which concerns on one Embodiment. It is a schematic diagram which shows the biological waste water treatment apparatus used for the phosphorus treatment which concerns on one Embodiment. It is a schematic diagram which shows the biological waste water treatment apparatus used for the nitrogen and phosphorus simultaneous processing (A2O method) which concerns on one Embodiment. It is a schematic diagram which shows the biological waste water treatment apparatus used for the nitrogen and phosphorus simultaneous processing (AOAO method) which concerns on one Embodiment. It is a schematic diagram which shows the biological waste water treatment apparatus used for the nitrogen and phosphorus simultaneous processing (A2O method) which concerns on one Embodiment. It is a schematic diagram which shows the biological waste water treatment apparatus used for the nitrogen and phosphorus simultaneous processing (AOAO method) which concerns on one Embodiment. It is a schematic diagram which shows the biological waste water treatment apparatus used for the anaerobic process which concerns on one Embodiment. It is a schematic diagram which shows the biological waste water treatment apparatus used for the anaerobic process which concerns on one Embodiment. It is a graph which shows temporal stability of a signal substance.

  Hereinafter, although the suitable form for implementing this invention is demonstrated in detail, this invention is not limited to these embodiment.

(Treatment bacteria)
Treatment bacteria include Streptomyces, Actinomyces, Mycobacterium, Rhodococcus, Corynebacterium, Micrococcus and other actinomycetes, Lactobacillus, Bifidobacterium, Lactococcus, Enterococcus, Pediococcus, Leuconostoc Lactic acid bacteria such as Aspergillus, Penicillium, Rhizopus, natto bacteria such as Bacillus, yeasts such as Saccharomyces, Schizosaccharomyces, methane, Ets. Nitrifying bacteria such as fungi, nitrosomonas, nitrosococcus, nitrosospira, nitrosovibrio, nitrobacter, nitrospira, nitrococcus, escherichia, enterobacter, klebsiella, bradyrizobium, a Karigenesu, Achromobacter, Azospirillum, Paracoccus, Pseudomonas, Rhodopseudomonas, include denitrificans such as Thiobacillus.

(Signal substance producing bacteria)
Examples of the signal substance-producing bacteria include Paracoccus, Burkholderia, Pseudomonas, Vibrio, Aeromonas, Bacillus, Streptomyces, Streptococcus, and Lactobacillus. The above bacteria include anaerobic bacteria and aerobic bacteria, and those skilled in the art can appropriately select them according to the purpose.

  The signal substance-producing bacterium may be in a form supported on a carrier or a membrane.

  As the carrier, a porous material such as a gel, a sponge, a ceramic, a synthetic resin, or the like formed into a cube shape, a rectangular parallelepiped shape, a cylindrical shape, a spherical shape, a corrugated plate shape, a tube shape, or the like can be suitably used. . In addition, as the membrane, an organic or inorganic filtration membrane formed into a planar shape or a tube shape can be used. Of the microorganisms present in the carrier and in the membrane, 50% or more, more preferably 70% or more, and even more preferably 90% or more of the microorganisms are preferably signal substance-producing bacteria.

  Further, the signal substance-producing bacterium may be Paracoccus AS6 strain or AS13 strain. When Paracoccus AS6 strain or AS13 strain is used, more N-hexadecanoyl homoserine lactone (C16-HSL) can be released in the solution, and more than half of the N-hexadecanoyl homoserine lactone is secreted in the state of being encapsulated in vesicles. Therefore, the signal substance can be efficiently delivered to the treated bacterium. Moreover, since AS6 strain | stump | stock and AS13 strain | stump | stock were isolated from activated sludge, they can survive in activated sludge.

(Signal substance)
Signal substances include N-acyl-L-homoserine lactones, AI2 (4,5-dihydroxy-2,3-pentanedione), HHQs (2-alkyl-4-quinolone), PQSs (2-alkyl- Quinolones and quinolines such as 3-hydroxy-4-quinolone), indoles, peptides, cyclic dipeptides, diketopiperazines and the like. Examples of N-acyl-L-homoserine lactones include N-butanoyl-L-homoserine lactone, N- (3-oxobutanoyl) -L-homoserine lactone, and N- (3-hydroxybutanoyl) -L-. Homoserine lactone, N-pentanoyl-L-homoserine lactone, N- (3-oxopentanoyl) -L-homoserine lactone, N- (3-hydroxypentanoyl) -L-homoserine lactone, N-hexanoyl-L-homoserine lactone N- (3-oxohexanoyl) -L-homoserine lactone, N- (3-hydroxyhexanoyl) -L-homoserine lactone, N-heptanoyl-L-homoserine lactone, N- (3-oxoheptanoyl)- L-homoserine lactone, N- (3-hydroxyheptanoyl) -L-homose Lactone, N-octanoyl-L-homoserine lactone, N- (3-oxooctanoyl) -L-homoserine lactone, N- (3-hydroxyoctanoyl) -L-homoserine lactone, N-nonanoyl-L-homoserine lactone, N- (3-oxononanoyl) -L-homoserine lactone, N- (3-hydroxynonanoyl) -L-homoserine lactone, N-decanoyl-L-homoserine lactone, N- (3-oxodecanoyl) -L-homoserine lactone, N- (3-hydroxydecanoyl) -L-homoserine lactone, N-undecanoyl-L-homoserine lactone, N- (3-oxoundecanoyl) -L-homoserine lactone, N- (3-hydroxyundecanoyl) -L-homoserine lactone, N-dodecanoyl-L-homo Phosphorus lactone, N- (3-oxododecanoyl) -L-homoserine lactone, N- (3-hydroxydodecanoyl) -L-homoserine lactone, N-tridecanoyl-L-homoserine lactone, N- (3-oxotridecanoyl) ) -L-homoserine lactone, N- (3-hydroxytridecanoyl) -L-homoserine lactone, N-tetradecanoyl-L-homoserine lactone, N- (3-oxotetradecanoyl) -L-homoserine lactone, N- (3-hydroxytetradecanoyl) -L-homoserine lactone, N-pentadecanoyl-L-homoserine lactone, N- (3-oxopentadecanoyl) -L-homoserine lactone, N- (3-hydroxypenta Decanoyl) -L-homoserine lactone, N-hexadecanoyl-L-homose N-acyl- having 4 to 16 carbon atoms in the acyl group such as phosphorus lactone, N- (3-oxohexadecanoyl) -L-homoserine lactone, N- (3-hydroxyhexadecanoyl) -L-homoserine lactone Examples include, but are not limited to, L-homoserine lactone.

(Biological wastewater treatment equipment)
The biological wastewater treatment apparatus according to the present embodiment includes a signal substance production unit that produces a signal substance with a signal substance-producing bacterium, and a treatment part that performs biological treatment of wastewater with the treatment bacterium.

  In the treatment unit, the wastewater is biologically treated (hereinafter, also referred to as “biological wastewater treatment”) with the treated bacteria. The biological waste water treatment in the processing section is performed in the presence of the signal substance produced in the signal substance production section. Biological wastewater treatment includes aerobic treatment and anaerobic treatment, and examples include denitrification treatment, nitrification treatment, partial nitrification treatment, anammox treatment, dephosphorization treatment, methane fermentation treatment, and organic acid generation treatment. Examples of the wastewater include organic wastewater (polluting substances) such as industrial wastewater, sewage, human waste, leachate, wastewater, and livestock manure.

  In the signal substance production department, a signal substance is produced by a signal substance producing bacterium. The signal substance production unit has a supply means for supplying the signal substance to the treated bacteria.

  In one embodiment, the biological wastewater treatment apparatus has a signal substance production unit outside the treatment unit. In this case, examples of the supply unit include piping connected to the processing unit and the signal substance production unit, and overflow from the signal material production unit to the processing unit. In the embodiment in which the signal substance production department exists outside the processing section, when there are a plurality of processing sections, the signal substance may be supplied to one or two or more processing sections whose processing efficiency is to be increased by the supply means. You may supply a signal substance to all the process parts.

  In one embodiment, the biological wastewater treatment apparatus has a signal substance production unit inside the treatment unit. In this case, examples of the supply means include diffusion by treated water (drainage) in the signal substance production department. In addition, the signal substance production section may be isolated from the processing section with a material that can pass the signal substance such as a semipermeable membrane and a filter but cannot pass the signal substance producing bacteria, and the signal substance production section and the processing section. May be the same. When the signal substance production part and the treatment part are the same, for example, the treated bacteria and the signal substance production bacteria coexist in the reaction tank or the like.

  The biological wastewater treatment apparatus may further include a signal substance producing bacterium production unit. The signal substance-producing bacterium production unit produces a signal substance-producing bacterium, and supplements the signal substance production bacterium with the signal substance-producing bacterium. By replenishing the signal substance producing bacteria to the signal substance producing department, the amount of the signal substance supplied from the signal substance producing department to the treated bacteria is stabilized, so that biological treatment of wastewater by the treated bacteria can be further promoted. . Replenishment of the signal substance producing bacterium to the signal substance production part may be performed by adding a product produced in a place different from the biological waste water treatment apparatus to the signal substance production part. Further, when the signal substance-producing bacteria are supported on a carrier, the signal substance-producing bacteria production part may produce a carrier on which the signal substance-producing bacteria are supported, and the carrier may be added to the signal substance production part. Alternatively, a carrier carrying a signal substance-producing bacterium may be produced at a location different from the biological waste water treatment apparatus, and the carrier may be added to the signal substance production unit.

  The timing of replenishing the signal substance producing bacteria to the signal substance producing part is, for example, the concentration of the object to be treated in the waste water discharged from the treating part, the concentration of the signal substance in the treating part, the signal substance producing bacteria in the signal substance producing part, The density or the like can be determined as an index. The said density | concentration, a density, etc. can be determined by the conventional method in this technical field. In addition, numerical values such as the concentration and density serving as an index may be appropriately set according to the type of the object to be processed, the type of the signal substance, the type of the signal substance producing bacteria, and the like.

  In one embodiment, the signal substance producing unit includes a signal substance producing bacterium and a substrate for producing the signal substance by the signal substance producing bacterium, and within the signal substance producing unit, maintaining the signal substance producing bacteria and Perform production. Note that the signal substance production unit is not for storing a signal substance produced outside. This is because storing the signal substance produced externally decreases the content of the signal substance, and a sufficient effect cannot be obtained.

  In one embodiment, the signal substance production section may have a substrate supply means for supplying a substrate for producing a signal substance by the signal substance producing bacteria. The substrate is not particularly limited as long as it can support the survival of the signal substance-producing bacteria and the production of the signal substance by the signal substance-producing bacteria, and examples thereof include carbon sources, vitamins, inorganic salts, nitrogen sources, and trace elements.

(Aerobic treatment)
The aerobic treatment is a method in which various aerobic bacteria are involved in the presence of dissolved oxygen to oxidatively decompose and remove organic substances, ammonia nitrogen, odor, iron and the like.

  FIG. 1 is a schematic diagram illustrating a biological wastewater treatment apparatus used for aerobic treatment according to an embodiment. A biological wastewater treatment apparatus 100 shown in FIG. 1A includes a reaction vessel (treatment unit) 110, a tank (signal substance production unit) 160, and a pipe (supply means) connected to the reaction vessel 110 and the tank 160. L160 and L110. The biological waste water treatment apparatus 100 shown in FIG. 1 (B) includes a final sedimentation basin 150 connected to the reaction tank 110 via a piping L130, and a final sedimentation basin 150 and a piping L150 connected to the piping L110. In addition.

  The signal substance production unit 160 may be connected to a signal substance production bacteria production unit (not shown) via a pipe L1601. The signal substance-producing bacterium production unit is, for example, a tank. Further, the signal substance production unit 160 may have a substrate supply means (not shown) for supplying a substrate for producing a signal substance by the signal substance producing bacteria.

  The aerobic process is performed, for example, with an apparatus as shown in FIG. As the aerobic treatment process, for example, water to be treated (drainage) is introduced into the reaction tank 110 through the pipe L110. At this time, as shown in FIG. 1A, by supplying the signal substance produced in the signal substance production unit 160 through the pipes L160 and L110, the signal substance is mixed with the water to be treated and the treated bacteria, and the waste water treatment. Is promoted. The connection destination of the pipe L160 may be the pipe L110 or the reaction tank 110. Furthermore, as shown in FIG. 1 (B), in the aspect provided with the final sedimentation basin 150 after the reaction tank 120, a part of the activated sludge that has flowed out together with the water to be treated is settled, and returned to the reaction tank through the pipe L150. You may return to 110.

  FIG. 2 is a schematic diagram showing a biological wastewater treatment apparatus used for aerobic treatment according to an embodiment. A biological wastewater treatment apparatus 200 shown in FIG. 2A includes a reaction vessel 210 that serves as both a treatment unit and a signal substance production unit. The biological waste water treatment apparatus 200 shown in FIG. 2 (B) includes a final sedimentation basin 250 connected to the reaction tank 210 via a piping L220, and a piping L250 connected to the final sedimentation basin 250 and the piping L210. In addition.

  The reaction vessel (signal substance producing unit) 210 may be connected to a signal substance producing bacteria producing unit (not shown). Moreover, the reaction tank (signal substance production part) 210 may have a substrate supply means (not shown) for supplying a substrate for producing a signal substance by the signal substance producing bacteria.

  In the biological wastewater treatment apparatus 200, the treated bacteria and the signal substance producing bacteria coexist in the reaction tank 210 that serves as both the treating part and the signal substance producing part. The signal substance produced by the signal substance producing bacteria is supplied to the treated bacteria by diffusion using the water to be treated as a medium. The signal substance-producing bacteria may be floating cells, or may be fixed in a carrier and maintained in the sludge of the reaction tank 210. Thereby, it becomes easy to maintain a signal substance producing microbe for a longer period of time.

  As shown in FIG. 2 (B), a mode in which a final sedimentation basin 250 is provided after the reaction tank 210 and a part of the activated sludge can be returned to the reaction tank 210 as return sludge through the pipe L250 may be employed.

(Membrane separation activated sludge method)
The membrane separation activated sludge method (MBR: Membrane Bio Reactor) is a method for reducing the size of equipment and processing performance by treating the solid-liquid separation of activated sludge with a microfiltration membrane or ultrafiltration membrane. It can be used not only for processing but also for anaerobic processing. Examples of the membrane separation activated sludge method include an integral immersion type, a separate tank immersion type, and an outside tank type.

  The integral soaking type membrane separation activated sludge method is an embodiment of the membrane separation activated sludge method using a reaction tank in which a microfiltration membrane or an ultrafiltration membrane is installed inside the reaction tank. It can filter with a microfiltration membrane or an ultrafiltration membrane. Moreover, in the tank separation immersion type membrane separation activated sludge method, the inside of the reaction tank is divided into two or more. In one section, wastewater treatment is performed with activated sludge, and in the other section, a microfiltration membrane or It is the aspect of the membrane separation activated sludge method which filters a to-be-processed water with an ultrafiltration membrane. The membrane separation activated sludge method outside the tank is equipped with a microfiltration membrane or ultrafiltration membrane connected to the reaction tank by piping outside the reaction tank, and is treated by the microfiltration membrane or ultrafiltration membrane. It is the aspect of the membrane separation activated sludge method which filters water.

  FIG. 3 is a schematic view showing a biological wastewater treatment apparatus used in the membrane separation activated sludge method according to one embodiment. A biological wastewater treatment apparatus 300 shown in FIG. 3 (A) is a biological wastewater treatment apparatus used for the integral immersion type membrane separation activated sludge method, and includes a reaction tank (treatment section) 310 and a tank (signal substance production). Part) 360, pipes (supply means) L360 and L310 connected to the reaction vessel 310 and the tank 360, and a filtration membrane 370 disposed inside the reaction vessel 310. A biological wastewater treatment apparatus 300 shown in FIG. 3 (B) is a biological wastewater treatment apparatus used for a tank-separated immersion type membrane separation activated sludge method, and a reaction tank having two sections 3101 and 3102; A tank (signal substance production section) 360, a reaction tank section 3101, a pipe (supply means) L 360 connected to the tank 360, and a filtration membrane 370 disposed inside the reaction tank section 3102 are provided. A biological wastewater treatment apparatus 300 shown in FIG. 3C is a biological wastewater treatment apparatus used for an outside tank type membrane separation activated sludge method, and includes a reaction tank (treatment section) 310 and a tank (signal substance production). Part) 360, pipes (supply means) L360 and L310 connected to the reaction tank 310 and the tank 360, and a filtration membrane 370 connected to the reaction tank 310 and the pipe L320.

  The signal substance production part 360 may be connected to a signal substance producing bacteria production part (not shown) via a pipe L3601. The signal substance-producing bacterium production unit is, for example, a tank. Moreover, the signal substance production part 360 may have a substrate supply means (not shown) for supplying a substrate for producing a signal substance by the signal substance producing bacteria.

  The membrane separation activated sludge method is performed, for example, with an apparatus as shown in FIG. As a process of the membrane separation activated sludge method, water to be treated (drainage) is introduced into the reaction tank 310 through the pipe L310. At this time, as shown in FIG. 3, by supplying the signal substance produced by the signal substance production unit 360 through the pipes L360 and L310, the signal substance is mixed with the water to be treated and the treatment bacteria, and the waste water treatment is promoted. The The connection destination of the pipe L360 may be the pipe L310 or the reaction vessel 310. In the reaction tank 310, an aerobic process or an anaerobic process is performed by a processing microbe. Subsequently, the water to be treated (drainage) is discharged through the pipe L340 after dust and activated sludge are removed by the filtration membrane 370. The filtration membrane 370 can be appropriately installed inside or outside the reaction vessel depending on the type of the membrane separation activated sludge method of the integral immersion type, the separate tank immersion type or the outside tank type. Further, as shown in FIG. 3B, a part of the activated sludge that has settled at the bottom of the reaction tank 3102 may be returned to the reaction tank 3101 through a pipe L350 as return sludge.

  FIG. 4 is a schematic diagram showing a biological wastewater treatment apparatus used in a membrane separation activated sludge method according to an embodiment. A biological wastewater treatment apparatus 400 shown in FIG. 4 includes a reaction tank 410 that serves as both a treatment unit and a signal substance production unit. A biological wastewater treatment apparatus 400 shown in FIG. 4 (A) is a biological wastewater treatment apparatus used in an integral immersion type membrane separation activated sludge method, and a reaction tank (treatment section and signal substance production section) 410, And a filtration membrane 470 disposed inside the reaction vessel 410. A biological wastewater treatment apparatus 400 shown in FIG. 4B is a biological wastewater treatment apparatus used in a tank-separated immersion type membrane separation activated sludge method, and is a reaction tank (treatment) having two sections 4101 and 4102. And a signal substance production department), and a filtration membrane 470 disposed inside the reaction tank section 4102. A biological wastewater treatment apparatus 400 shown in FIG. 4 (C) is a biological wastewater treatment apparatus used for an outside tank type membrane separation activated sludge method, and includes a reaction tank 410 (treatment section and signal substance production section), The filter membrane 470 connected with the reaction tank 410 with the piping L420 is provided.

  The reaction tank 410 may be connected to a signal substance producing bacteria production unit (not shown) through a pipe. The signal substance-producing bacterium production unit is, for example, a tank. Moreover, the reaction tank 410 may have a substrate supply means (not shown) for supplying a substrate for producing a signal substance by the signal substance producing bacteria.

  In the biological wastewater treatment apparatus 400, the treatment bacteria and the signal substance producing bacteria coexist in the reaction tank 410 that serves as the treatment part and the signal substance production part. The signal substance produced by the signal substance producing bacteria is supplied to the treated bacteria by diffusion using the water to be treated as a medium. The signal substance-producing bacteria may be floating cells, or may be fixed to a carrier and maintained in the sludge of the reaction tank 410. Thereby, it becomes easy to maintain a signal substance producing microbe for a longer period of time.

  In the reaction tanks 410, 4101 and 4102, an aerobic process or an anaerobic process is performed by the treatment bacteria. Subsequently, the water to be treated (drainage) is discharged through the pipe L440 after dust and activated sludge are removed by the filtration membrane 470. The filtration membrane 470 can be appropriately installed inside or outside the reaction vessel. Furthermore, as shown in FIG. 4B, a part of the activated sludge that has settled at the bottom of the reaction tank 4102 may be returned to the reaction tank 4101 through a pipe L450 as return sludge.

(Nitrogen treatment)
Nitrogen treatment is treatment that converts ammonia and nitrogen compounds in waste water into nitrogen gas and removes nitrogen compounds. Examples of the nitrogen treatment include nitrification / denitrification treatment, partial nitrification / anammox treatment.

(Nitrification / denitrification treatment)
In the nitrification / denitrification treatment, ammonia nitrogen is converted into nitrite nitrogen or nitrate nitrogen by denitrifying bacteria in a denitrification tank, and nitrate nitrogen is converted into nitrogen gas by nitrifying bacteria in the nitrification tank. On the other hand, in partial nitrification / anammox treatment, a part of ammonia nitrogen is converted into nitrite nitrogen by nitrifying bacteria in the reaction tank, and ammonia nitrogen and nitrite nitrogen are reacted anaerobically by specific microorganisms. This is a process of converting into nitrogen gas.

  FIG. 5 is a schematic diagram showing a biological wastewater treatment apparatus used for nitrogen treatment according to an embodiment. A biological wastewater treatment apparatus 500 shown in FIG. 5A is called a step inflow nitrification denitrification apparatus, and includes a tank (signal substance production section) 560, a denitrification tank (treatment section) 510, a nitrification tank ( Treatment section) 520, denitrification tank (treatment section) 530, nitrification tank (treatment section) 540, final sedimentation tank 550, signal substance production section 560 and piping (supply means) L560 connected to denitrification tank 510, and L510. Moreover, the biological waste water treatment apparatus 500 shown in FIG. 5B includes denitrification tanks or nitrification tanks 510, 520, 530, and 540 that serve as a processing section and a signal substance production section.

  The signal substance producing unit 560 may be connected to a signal substance producing bacteria producing unit (not shown) via a pipe L5601. The signal substance-producing bacterium production unit is, for example, a tank. In addition, the signal substance production unit 560 may include a substrate supply unit (not shown) that supplies a substrate for producing a signal substance by the signal substance producing bacteria.

  Nitrogen treatment is performed, for example, with an apparatus as shown in FIG. As a process of nitrogen treatment, for example, treated water (drainage) is introduced into the denitrification tank 510 through the pipe L510. At this time, as shown in FIG. 5A, by supplying the signal substance produced by the signal substance production unit 560 through the pipes L560 and L510, the signal substance is mixed with the water to be treated and the treatment bacteria, and the waste water treatment. Is promoted. The connection destination of the pipe L560 may be the pipe L510 or the denitrification tank 510. Alternatively, the pipe L510 may be branched to introduce the water to be treated into the denitrification tank 530 through the pipe 5102. Subsequently, water to be treated that has been treated through the denitrification tank 510, the nitrification tank 520, the denitrification tank 530, and the nitrification tank 540 is introduced into the final sedimentation tank 550. In the final sedimentation basin 550, a part of the activated sludge that has flowed out along with the water to be treated may be precipitated and returned to the denitrification tank 510 through the pipe L550 as return sludge. At this time, the connection destination of the pipe L550 may be the nitrification tank 520, the denitrification tank 530, or the nitrification tank 540.

  Drawing 5 (B) is a mimetic diagram showing the biological wastewater treatment equipment used for the membrane separation activated sludge method concerning one embodiment. A biological wastewater treatment apparatus 500 shown in FIG. 5B includes denitrification tanks or nitrification tanks 510, 520, 530, and 540 that serve as a processing section and a signal substance production section.

  The denitrification tanks or nitrification tanks 510, 520, 530, and 540 that serve as the processing section and the signal substance production section may be connected to a signal substance production bacteria production section (not shown) via a pipe. The signal substance-producing bacterium production unit is, for example, a tank. Further, the denitrification tanks or nitrification tanks 510, 520, 530 and 540 which serve as the processing part and the signal substance production part are provided with substrate supply means (not shown) for supplying a substrate for producing the signal substance by the signal substance producing bacteria. You may have.

  In the biological wastewater treatment apparatus 500, the treated bacteria and the signal substance producing bacteria coexist in the tanks 510, 520, 530 and 540 which serve as the treating part and the signal substance producing part. The signal substance produced by the signal substance producing bacteria is supplied to the treated bacteria by diffusion using the water to be treated as a medium. Further, for example, the signal substance produced in the denitrification tank 510 can be supplied to the nitrification tank 520 by overflow (supply means). The signal substance-producing bacteria may be floating cells, or may be fixed on a carrier and maintained in the sludge of the reaction vessels 510, 520, 530 and 540. Thereby, it becomes easy to maintain a signal substance producing microbe for a longer period of time.

  FIG. 6 is a schematic diagram showing a biological wastewater treatment apparatus used for nitrogen treatment according to an embodiment. A biological wastewater treatment apparatus 600 shown in FIG. 6A is called a circulating nitrification denitrification apparatus, and includes a tank (signal material production unit) 660, a denitrification tank (treatment unit) 610, and a nitrification tank (treatment). Part) 620, a final sedimentation tank 650, piping (supplying means) L660 and L610 connected to the denitrification tank 610 and the signal substance production part 660.

  The signal substance producing unit 660 may be connected to a signal substance producing bacteria producing unit (not shown) via a pipe L6601. The signal substance-producing bacterium production unit is, for example, a tank. Moreover, the signal substance production | generation part 660 may have the substrate supply means (not shown) which supplies the substrate for producing a signal substance with a signal substance production microbe.

  Nitrogen treatment may be performed, for example, with an apparatus as shown in FIG. As a process of nitrogen treatment, for example, treated water (drainage) is introduced into the denitrification tank 610 through the pipe L610. At this time, as shown in FIG. 6A, by supplying the signal substance produced by the signal substance production unit 660 through the pipes L660 and L610, the signal substance is mixed with the water to be treated and the treatment bacteria, and the waste water treatment. Is promoted. The connection destination of the pipe L610 may be the pipe L610 or the denitrification tank 610. Subsequently, the treated water treated in the denitrification tank 610 is introduced into the nitrification tank 620 by overflow, for example. Furthermore, the water to be treated that has been nitrified in the nitrification tank 620 is introduced into the final sedimentation basin 650 through the pipe L630. At this time, the water to be treated may be introduced again into the denitrification tank 610 through a pipe L6302 branched from the pipe L630 and circulated. The treated water introduced into the final sedimentation basin 650 precipitates waste and activated sludge and drains it through the pipe L640. Moreover, you may return a part of activated sludge settled to the bottom part of the final sedimentation tank 650 to the piping L610 as return sludge through the piping L650. The connection destination of the pipe L650 may be the denitrification tank 610.

  FIG. 6B is a schematic diagram showing a biological wastewater treatment apparatus used for nitrogen treatment according to one embodiment. A biological wastewater treatment apparatus 600 shown in FIG. 6B includes a denitrification tank 610 and a nitrification tank 620. The denitrification tank 610 and the nitrification tank 620 may serve as a processing unit and a signal substance production unit. Further, the signal substance production section may be either the denitrification tank 610 or the nitrification tank 620, or both.

  The denitrification tank 610 and the nitrification tank 620 that serve as the processing section and the signal substance production section may be connected to a signal substance production bacteria production section (not shown). Further, the denitrification tank 610 and the nitrification tank 620 that serve both as the processing section and the signal substance production section may have a substrate supply means (not shown) for supplying a substrate for producing the signal substance by the signal substance producing bacteria. Good.

  In the biological wastewater treatment apparatus 600, the treatment bacteria and the signal substance producing bacteria coexist in the denitrification tank 610 and the nitrification tank 620 that serve as the treatment section and the signal substance production section. The signal substance produced by the signal substance producing bacteria is supplied to the treated bacteria by diffusion using the water to be treated as a medium. Further, for example, the signal substance produced in the denitrification tank 610 can be supplied to the nitrification tank 620 by overflow (supply means). The signal substance-producing bacteria may be floating cells, or may be fixed to a carrier and maintained in the sludge of the denitrification tank 610 and the nitrification tank 620. Thereby, it becomes easy to maintain a signal substance producing microbe for a longer period of time.

(Partial nitrification / anammox treatment)
The partial nitrification reaction is a nitrification reaction that converts ammonia nitrogen to nitrite nitrogen, unlike the nitrification reaction that converts ammonia nitrogen to nitrate nitrogen. The anammox reaction means an anaerobic ammonia oxidation reaction, and ammonia-nitrogen can be directly converted into nitrogen gas by using nitrite-nitrogen as an electron acceptor under anoxic conditions. It is a biological process.

  FIG. 7 is a schematic diagram showing a biological wastewater treatment apparatus used for nitrogen treatment, particularly partial nitrification / anammox treatment. A biological wastewater treatment apparatus 700 shown in FIG. 7A includes a reaction tank (treatment section) 710, a tank (signal substance production section) 760, and piping connected to the reaction tank 710 and the signal substance production section 760 ( Supply means) L760 and L710. A biological wastewater treatment apparatus 700 shown in FIG. 7B further includes an anammox treatment tank 7102 connected to the reaction tank via a pipe L7101.

  The signal substance producing unit 760 may be connected to a signal substance producing bacteria producing unit (not shown) via a pipe L7601. The signal substance-producing bacterium production unit is, for example, a tank. Further, the signal substance production unit 760 may have a substrate supply unit (not shown) for supplying a substrate for producing a signal substance by the signal substance producing bacteria.

  The partial nitrification / anammox treatment is performed, for example, with an apparatus as shown in FIG. As a process of partial nitrification / anammox treatment, for example, water to be treated (drainage) is introduced into the reaction tank 710 through a pipe L710. At this time, as shown in FIG. 7A, by supplying the signal substance produced by the signal substance production unit 760 through the pipes L760 and L710, the signal substance is mixed with the water to be treated and the treatment bacteria, and the wastewater treatment. Is promoted. The connection destination of the pipe L760 may be the pipe L710 or the reaction tank 710. In the reaction tank 710, partial nitrification / anammox treatment is performed by the treatment bacteria. The water to be treated treated in the reaction tank 710 is discharged through the pipe L720.

  FIG. 7B is a schematic diagram showing a biological wastewater treatment apparatus 700 used for partial nitrification / anammox treatment according to an embodiment. A biological wastewater treatment apparatus 700 shown in FIG. 7B includes a reaction tank (treatment unit) 7101, a tank (signal substance production part) 760, and piping connected to the reaction tank 710 and the signal substance production part 760 ( Supply means) L760 and L710, and an anammox processing tank (processing section) 7102 connected to the reaction tank 7101 through a pipe L7101. The signal substance production unit 760 may further include a supply unit (not shown) for supplying a signal substance to the anammox treatment tank 7102.

  FIG. 8 is a schematic diagram showing a biological wastewater treatment apparatus used for partial nitrification / anammox treatment according to an embodiment. A biological wastewater treatment apparatus 800 illustrated in FIG. 8A includes a reaction tank 810. A biological wastewater treatment apparatus 800 shown in FIG. 8B further includes an anammox treatment tank 820 connected to the reaction tank 810 via a pipe L8101. The anammox treatment tank 820 and the reaction tank 810 may serve as a treatment section and a signal substance production section. In addition, the signal substance production unit may be either the anammox treatment tank 820 or the reaction tank 810, or both. The biological wastewater treatment apparatus 800 may branch the pipe L810 and introduce water to be treated (drainage) into the anammox treatment tank 820 through the pipe L8102.

  The reaction tank 810 and the anammox treatment tank 820 serving as a signal substance production section may be connected to a signal substance production bacteria production section (not shown). In addition, the reaction tank 810 and the anammox treatment tank 820 serving as a signal substance production unit may have a substrate supply means (not shown) for supplying a substrate for producing a signal substance by the signal substance producing bacteria.

  In the biological wastewater treatment apparatus 800, the treatment bacteria and the signal substance producing bacteria coexist in the reaction tank 810 or the anammox treatment tank 820 that serves as the treatment section and the signal substance production section. The signal substance produced by the signal substance producing bacteria is supplied to the treated bacteria by diffusion using the water to be treated as a medium. The signal substance-producing bacteria may be floating cells, or may be fixed to a carrier and maintained in the sludge of the reaction tank 810 or the Anammox treatment tank 820. Thereby, it becomes easy to maintain a signal substance producing microbe for a longer period of time.

(Phosphorus treatment)
Phosphorus treatment is a treatment for removing phosphorus compounds in waste water by utilizing the properties of dephosphorizing bacteria. The dephosphorizing bacteria are present in activated sludge and release phosphorus from the body in the absence of available oxygen. When oxygen becomes available, it has the property of taking in more phosphorus than the amount exhaled into the body. Utilizing this function, phosphorus compounds can be removed from the water to be treated by incorporating phosphorus in the water into the sludge.

  As shown in FIGS. 9 and 10, the biological wastewater treatment apparatus used for the phosphorus treatment includes an anaerobic tank and an aerobic tank. The inside of the anaerobic tank is kept in a state in which dissolved oxygen, nitrate ions and nitrite ions do not exist, and the dephosphorizing bacteria release phosphorus in the body inside the anaerobic tank. On the other hand, the inside of the aerobic tank is kept in a state where dissolved oxygen is present, and the dephosphorizing bacteria take up phosphorus in the water to be treated into the body inside the aerobic tank. Therefore, it is preferable that the aerobic tank is connected after the anaerobic tank.

  FIG. 9 is a schematic diagram showing a biological wastewater treatment apparatus used for phosphorus treatment according to an embodiment. A biological wastewater treatment apparatus 900 shown in FIG. 9 is connected to an anaerobic tank (processing unit) 910, an aerobic tank (processing unit) 920, a tank (signal substance production unit) 960, an anaerobic tank 910, and a tank 960. Piping (supplying means) L960 and L910. The biological waste water treatment apparatus 900 may further include a final sedimentation tank 950 connected to the aerobic tank 920 through the pipe L920, and a pipe L950 connected to the final sedimentation tank 950 and the pipe L910. .

  The signal substance production unit 960 may be connected to a signal substance production bacteria production unit (not shown) via a pipe L9601. The signal substance-producing bacterium production unit is, for example, a tank. Further, the signal substance production unit 960 may have a substrate supply unit (not shown) for supplying a substrate for producing a signal substance by the signal substance producing bacteria. The signal substance production unit 960 may further include supply means (not shown) for supplying the signal substance to the aerobic tank 920.

  The phosphorus treatment is performed by an apparatus as shown in FIG. 9, for example. As a process of phosphorus treatment, for example, treated water (drainage) is introduced into the anaerobic tank 910 through the pipe L910. At this time, as shown in FIG. 9, by supplying the signal substance produced by the signal substance production unit 960 through the pipes L960 and L910, the signal substance is mixed with the water to be treated and the treatment bacteria, and the waste water treatment is promoted. The The connection destination of the pipe L960 may be the pipe L910, the anaerobic tank 910, or the aerobic tank 920. The signal substance production unit 960 may be connected to both the anaerobic tank 910 and the aerobic tank 920, for example, by branching the pipe L960 or by a pipe (not shown) different from the pipe L960. Furthermore, in the aspect provided with the final sedimentation basin 950 after the aerobic tank 920, a part of activated sludge which flowed out with the to-be-processed water may be settled, and you may return to the anaerobic tank 910 through the piping L950 as return sludge. Subsequently, the water to be treated that has been subjected to anaerobic treatment in the anaerobic tank 910 is sent to the aerobic tank 920 and subjected to phosphorus treatment by dephosphorizing bacteria. The treated water that has been subjected to the phosphorus treatment is sent to the final sedimentation basin 950, and the supernatant water is discharged through the pipe L940.

  FIG. 10 is a schematic diagram showing a biological wastewater treatment apparatus used for phosphorus treatment according to an embodiment. A biological wastewater treatment apparatus 1000 illustrated in FIG. 10 includes an anaerobic tank 1010 and an aerobic tank 1020. The anaerobic tank 1010 and the aerobic tank 1020 may serve as a processing unit and a signal substance production unit. The signal substance production unit may be either one of the anaerobic tank 1010 or the aerobic tank 1020, or both.

  The anaerobic tank 1010 and the aerobic tank 1020 serving as the signal substance producing unit may be connected to a signal substance producing bacteria producing unit (not shown). Moreover, the anaerobic tank 1010 and the aerobic tank 1020 which are signal substance production parts may have a substrate supply means (not shown) for supplying a substrate for producing a signal substance by a signal substance producing bacterium.

  In the biological wastewater treatment apparatus 1000, the treatment bacteria and the signal substance producing bacteria coexist in the anaerobic tank 1010 or the aerobic tank 1020 which serves as the treatment part and the signal substance production part. The signal substance produced by the signal substance producing bacteria is supplied to the treated bacteria by diffusion using the water to be treated as a medium. The signal substance-producing bacteria may be floating cells, or may be fixed to a carrier and maintained in the sludge of the anaerobic tank 1010 or the aerobic tank 1020. Thereby, it becomes easy to maintain a signal substance producing microbe for a longer period of time.

  In the anaerobic tank 1010, it is preferable to select an anaerobic bacterium as a treatment bacterium or a signal substance producing bacterium. In the aerobic tank 1020, it is preferable to select aerobic bacteria as treatment bacteria and signal substance producing bacteria.

(Nitrogen and phosphorus simultaneous treatment)
Examples of the nitrogen and phosphorus simultaneous treatment include an A2O method and an AOAO method. The A2O method is a method in which treated water is treated in the order of an anaerobic tank, an anaerobic tank, and an aerobic tank. The AOAO method is a method of covering an anaerobic tank, an aerobic tank, an anaerobic tank, and an aerobic tank in this order. This is a method in which treated water is treated. Here, nitrate ions and nitrite ions may be present inside the oxygen-free tank, but dissolved oxygen is not present. The anaerobic tank and the aerobic tank are as described above.

  FIG. 11 is a schematic view showing a biological wastewater treatment apparatus used for nitrogen and phosphorus simultaneous treatment, particularly A2O method, according to an embodiment. The biological wastewater treatment apparatus 1100 includes an anaerobic tank (processing section) 1110, an anaerobic tank (processing section) 1120, an aerobic tank (processing section) 1130, a tank (signal substance production section) 1160, and a signal substance. Pipes (supply means) L1160 and L1110 connected to the production unit 1160 and the anaerobic tank 1110 are provided. The signal substance production unit 1160 may have a supply means (not shown) for supplying a signal substance to the anoxic tank 1120 or the aerobic tank 1130.

  The activated sludge used in the A2O method includes dephosphorization bacteria that release and take up phosphorus, denitrification bacteria that convert nitrate nitrogen to nitrogen gas, and nitrification that converts ammonia nitrogen to nitrite nitrogen or nitrate nitrogen There are fungi. In the anaerobic tank 1110, the dephosphorizing bacteria release phosphorus from the body. In the anoxic tank 1120, denitrifying bacteria perform a denitrifying reaction to convert nitrate nitrogen into nitrogen gas. Further, air is introduced into the aerobic tank 1130. In the aerobic tank 1130, dephosphorization bacteria take in phosphorus in the water to be treated, while nitrifying bacteria perform nitrification reaction, and ammonia nitrogen is converted into nitrous acid. Convert to nitrogen or nitrate nitrogen. By circulating these three tanks, phosphorus treatment and nitrogen treatment can be performed simultaneously. Each tank may be equipped with a stirrer for the purpose of stirring.

  The signal substance producing unit 1160 may be connected to a signal substance producing bacteria producing unit (not shown) via the pipe L1161. The signal substance-producing bacterium production unit is, for example, a tank. Further, the signal substance production unit 1160 may have a substrate supply means (not shown) for supplying a substrate for producing a signal substance by the signal substance producing bacteria.

  Nitrogen and phosphorus simultaneous processing is performed by an apparatus as shown in FIG. 11, for example. As a process of nitrogen and phosphorus simultaneous treatment, for example, treated water (drainage) is introduced into the anaerobic tank 1110 through the pipe L1110. At this time, by supplying the signal substance produced by the signal substance production unit 1160 through the pipes L1160 and L1110, the signal substance is mixed with the water to be treated and the treatment bacteria, and the waste water treatment is promoted. The connection destination of the pipe L1160 may be the pipe L1110, the anaerobic tank 1110, the anoxic tank 1120, or the aerobic tank 1130. The signal substance production unit 1160 may be connected to at least one of the anaerobic tank 1110, the anaerobic tank 1120, and the aerobic tank 1130, or may be connected to all three. Furthermore, in the aspect provided with the final sedimentation basin 1150 after the aerobic tank 1130, a part of activated sludge which flowed out with the to-be-processed water may be settled, and you may return to the anaerobic tank 1110 through the piping L1150 as return sludge.

  FIG. 12 is a schematic view showing a biological wastewater treatment apparatus used for simultaneous nitrogen and phosphorus treatment, particularly AOAO method, according to one embodiment. A biological wastewater treatment apparatus 1200 shown in FIG. 12 includes a tank (signal substance production unit) 1260, an anaerobic tank (processing unit) 1210, an aerobic tank (processing unit) 1220, and an anaerobic tank (processing unit) 1230. And an aerobic tank (processing unit) 1240 (also referred to as a re-aeration tank), and pipes (supply means) L1260 and L1210 connected to the signal substance production unit 1260 and the anaerobic tank 1210. The signal substance production unit 1260 may include supply means (not shown) for supplying a signal substance to the aerobic tank 1220, the anoxic tank 1230, or the aerobic tank 1240.

  In the biological wastewater treatment apparatus 1200, the dephosphorizing bacteria release phosphorus from the body to the water to be treated in the anaerobic tank 1210. In the aerobic tank 1220, dephosphorizing bacteria take phosphorus from the water to be treated into the body, while nitrifying bacteria convert ammonia nitrogen into nitrate nitrogen. Further, in the anoxic tank 1230, the denitrifying bacteria perform a denitrifying reaction to convert nitrate nitrogen into nitrogen gas, and the dephosphorizing bacteria take up phosphorus into the body in an aerobic tank (also referred to as a re-aeration tank). In the anaerobic tank 1210, a denitrification reaction may be performed. Here, the signal substance produced by the signal substance production unit 1260 is introduced into the waste water treatment apparatus through the pipe (supply means) L1260, and the connection destination of the pipe (supply means) L1260 is the pipe L1210, the anaerobic tank 1210, and the like. At least one of the air tank 1220, the oxygen-free tank 1230, and the aerobic tank 1240 may be used, and two or more may be used.

  The signal substance producing unit 1260 may be connected to a signal substance producing bacteria producing unit (not shown) via the pipe L1261. The signal substance-producing bacterium production unit is, for example, a tank. Further, the signal substance production unit 1260 may have a substrate supply means (not shown) for supplying a substrate for producing a signal substance by the signal substance producing bacteria.

  FIG. 13 is a schematic diagram showing a biological wastewater treatment apparatus used for nitrogen and phosphorus simultaneous treatment, particularly A2O method, according to one embodiment. A biological wastewater treatment apparatus 1300 shown in FIG. 13 includes an anaerobic tank 1310, an oxygen-free tank 1320, and an aerobic tank 1330. The anaerobic tank 1310, the oxygen-free tank 1320, and the aerobic tank 1330 may serve as the processing unit and the signal substance production unit. The signal substance production section may be at least one of the anaerobic tank 1310, the anaerobic tank 1320, and the aerobic tank 1330, and may be all three. The biological waste water treatment apparatus 1300 further includes a final sedimentation basin 1350 connected to the anaerobic tank 1330 via a piping L1320, and a piping L1350 coupled to the final sedimentation basin 1350 and the piping L1310.

  The anaerobic tank 1310, the oxygen-free tank 1320, or the aerobic tank 1330 serving as the signal substance production unit may be connected to a signal substance production bacteria production unit (not shown). Further, the anaerobic tank 1310, the anaerobic tank 1320 or the aerobic tank 1330 serving as the signal substance production unit has a substrate supply means (not shown) for supplying a substrate for producing the signal substance by the signal substance producing bacteria. It may be.

  In the biological wastewater treatment apparatus 1300, the treatment bacteria and the signal substance producing bacteria coexist in the anaerobic tank 1310, the anoxic tank 1320, or the aerobic tank 1330 that serves as the treatment unit and the signal substance production unit. The signal substance produced by the signal substance producing bacteria is supplied to the treated bacteria by diffusion using the water to be treated as a medium. Further, for example, the signal substance produced in the anaerobic tank 1310 can be supplied to the anoxic tank 1320 by overflow (supply means). The signal substance-producing bacteria may be floating cells, or may be fixed in a carrier and maintained in sludge in the anaerobic tank 1310, anoxic tank 1320, or aerobic tank 1330. Thereby, it becomes easy to maintain a signal substance producing microbe for a longer period of time.

  FIG. 14 is a schematic diagram showing a biological wastewater treatment apparatus used for simultaneous nitrogen and phosphorus treatment, particularly AOAO method, according to one embodiment. A biological wastewater treatment apparatus 1400 shown in FIG. 14 includes an anaerobic tank 1410, an aerobic tank 1420, an anaerobic tank 1430, and an aerobic tank 1440. The anaerobic tank 1410, the aerobic tank 1420, the anaerobic tank 1430, and the aerobic tank 1440 may serve as a processing unit and a signal substance production unit. The signal substance production unit may be at least one of the anaerobic tank 1410, the aerobic tank 1420, the anaerobic tank 1430, and the aerobic tank 1440, and may be all four. The biological wastewater treatment apparatus 1400 further includes a final sedimentation basin 1450 connected to the anaerobic tank 1440 via a piping L1420, and a piping L1450 connected to the final sedimentation basin 1450 and the piping L1410.

  The anaerobic tank 1410, the aerobic tank 1420, the anaerobic tank 1430, or the aerobic tank 1440 serving as the signal substance production unit may be connected to a signal substance producing bacteria production unit (not shown). The anaerobic tank 1410, the aerobic tank 1420, the anaerobic tank 1430, or the aerobic tank 1440 serving as a signal substance production unit is a substrate supply means (not shown) for supplying a substrate for producing a signal substance by a signal substance producing bacterium. May be included).

  In the biological wastewater treatment apparatus 1400, the treatment bacteria and the signal substance producing bacteria coexist in the anaerobic tank 1410, the aerobic tank 1420, the anoxic tank 1430 or the aerobic tank 1440 that serves as the treatment unit and the signal substance production unit. . The signal substance produced by the signal substance producing bacteria is supplied to the treated bacteria by diffusion using the water to be treated as a medium. Further, for example, the signal substance produced in the anaerobic tank 1410 can be supplied to the aerobic tank 1420 by overflow (supply means). The signal substance-producing bacteria may be suspended cells or may be fixed to a carrier and maintained in sludge in the anaerobic tank 1410, the aerobic tank 1420, the anaerobic tank 1430, or the aerobic tank 1440. Thereby, it becomes easy to maintain a signal substance producing microbe for a longer period of time.

  As shown in FIG. 14, a mode in which a final sedimentation tank 1450 is provided after the aerobic tank 1440 and a part of the activated sludge can be returned to the pipe L1410 as return sludge through the pipe L1450. The connection destination of the pipe L1450 may be the anaerobic tank 1410.

(Anaerobic treatment)
An anaerobic process is a process which converts an organic compound into methane or carbon dioxide by hydrolysis, acid generation, methane fermentation or the like. Most organic compounds in the water to be treated are converted to methane via acetic acid and lower fatty acids, but may be converted to methane without going through the organic acid. The acid generation treatment is a treatment for hydrolyzing polymers such as polysaccharides and proteins in water to be treated into monomers such as monosaccharides and amino acids and converting them into acetic acid and lower fatty acids in an acid generation tank. The methane fermentation treatment is a treatment in which acetic acid and lower fatty acids in the treated water are converted into methane, carbon dioxide, water, or the like by anaerobic bacteria and removed from the treated water in an anaerobic tank.

  FIG. 15 is a schematic diagram illustrating a biological wastewater treatment apparatus used for anaerobic treatment according to an embodiment. A biological wastewater treatment apparatus 1500 shown in FIG. 15 is connected to an acid generation tank 1510, an anaerobic tank (treatment section) 1520, a tank (signal substance production section) 1560, a signal substance production section 1560, and an anaerobic tank 1520. Piping (supply means) L1560 and L1520.

  The signal substance producing unit 1560 may be connected to a signal substance producing bacteria producing unit (not shown) via a pipe L1561. The signal substance-producing bacterium production unit is, for example, a tank. Further, the signal substance production unit 1560 may have a substrate supply means (not shown) for supplying a substrate for producing the signal substance by the signal substance producing bacteria.

  The anaerobic process is performed by, for example, an apparatus as shown in FIG. As an anaerobic treatment step, for example, treated water (drainage) is introduced into the acid generation tank 1510 through a pipe L1510. Subsequently, the water to be treated that has undergone the acid generation treatment is introduced into the anaerobic tank 1520 through the pipe L1520. At this time, by supplying the signal substance produced by the signal substance production unit 1560 through the pipes L1560 and L1520, the signal substance is mixed with the water to be treated and the treatment bacteria, and the wastewater treatment is promoted. The connection destination of the pipe L1560 may be the pipe L1520 or the reaction tank 1520. Furthermore, the water to be treated that has been anaerobically treated is discharged through the pipe L1540.

  FIG. 16 is a schematic diagram illustrating a biological wastewater treatment apparatus used for anaerobic treatment according to an embodiment. A biological wastewater treatment apparatus 1600 shown in FIG. 16 includes an acid generation tank 1610 and an anaerobic tank 1660 that doubles as a processing unit and a signal substance production unit.

  The anaerobic tank (signal substance producing unit) 1660 may be connected to a signal substance producing bacteria producing unit (not shown). Moreover, the anaerobic tank (signal substance production part) 1660 may have a substrate supply means (not shown) for supplying a substrate for producing a signal substance by the signal substance producing bacteria.

  In the biological wastewater treatment apparatus 1600, the treated bacteria and the signal substance producing bacteria coexist in the anaerobic tank (signal substance producing unit) 1660. The signal substance produced by the signal substance producing bacteria is supplied to the treated bacteria by diffusion using the water to be treated as a medium. The signal substance-producing bacteria may be floating cells, or may be fixed in a carrier and maintained in sludge in the anaerobic tank 1660. Thereby, it becomes easy to maintain a signal substance producing microbe for a longer period of time.

The present inventors conducted experiments as follows, and found that the content of the signal substance rapidly decreases when the signal substance is stored in water.
(1) 2 mL of pure water was put into a vial, and butyryl-L-homoserine lactone was added so that the final concentration was 90 μM.
(2) The solution obtained in (1) was allowed to stand at 30 ° C., sampled over time, and the concentration of butyryl-L-homoserine lactone was measured by HPLC.

Measurement condition column: TSK-GEL octyl-80TS (4.6 mm × 25 cm)
Mobile phase: water: acetonitrile = 75: 25
Flow rate: 1.0 mL / min Detector: Ultraviolet light (195 nm)

  The results are shown in FIG. Butyryl-L-homoserine lactone decreased to 70% of the initial concentration after 5 hours and was 35% after 24 hours.

  100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600 ... biological wastewater treatment equipment, 110, 210, 310, 3101, 3102 , 410, 4101, 4102, 710, 810 ... reaction tank, 510, 530, 610 ... denitrification tank, 520, 540, 620 ... nitrification tank, 7101, 810 ... partial nitrification tank, 7102, 820: Anammox reaction tank, 910, 1010, 1110, 1210, 1310, 1410 ... Anaerobic tank, 920, 1020, 1130, 1220, 1240, 1330, 1420, 1440 ... Aerobic tank, 1120, 1230 , 1320, 1430 ... anoxic tank, 1510, 1610 ... acid generation tank, 20, 1660 ... anaerobic tank, 160, 260, 360, 460, 560, 660, 760, 860, 960, 1060, 1160, 1260, 1360, 1460, 1560, 1660 ... signal substance production department, L160, L360, L560, L660, L760, L960, L1160, L1260, L1560 ... supply means.

Claims (11)

A signal substance producing section for producing a signal substance by a signal substance producing bacterium;
A treatment unit that performs biological treatment of wastewater by the treatment bacteria,
The signal substance production unit has supply means for supplying the signal substance to the treated bacteria.
Biological wastewater treatment equipment.   The biological wastewater treatment apparatus according to claim 1, wherein the signal substance production unit exists outside the treatment unit.   The biological wastewater treatment apparatus according to claim 1, wherein the signal substance production unit is present inside the treatment unit.   The biological wastewater treatment apparatus according to any one of claims 1 to 3, wherein the signal substance-producing bacterium is in the form of suspended cells or in a supported form.   The biological wastewater according to any one of claims 1 to 4, wherein the signal substance-producing bacterium is Paracoccus AS6 strain (Accession No. NITE P-1363) or Paracoccus AS13 strain (Accession No. NITE P-1364). Processing equipment. A biological wastewater treatment method using the biological wastewater treatment apparatus according to claim 1,
A step of producing a signal substance by the signal substance producing bacteria in the signal substance production section;
Supplying the signal substance to the treated bacteria by the supply means;
A biological wastewater treatment method including a step of performing biological treatment of wastewater by the treatment bacteria in the presence of the signal substance in the treatment section.   The biological wastewater treatment method according to claim 6, wherein the signal substance production unit exists outside the treatment unit.   The biological wastewater treatment method according to claim 6, wherein the signal substance production unit is present inside the treatment unit.   The biological wastewater treatment method according to any one of claims 6 to 8, wherein the signal substance-producing bacterium is in the form of floating cells or a supported form.   The biological wastewater treatment method according to any one of claims 6 to 9, further comprising a step of supplementing the signal substance production unit with the signal substance producing bacteria.   The method according to any one of claims 6 to 10, wherein the signal substance-producing bacterium is Paracoccus AS6 strain (Accession No. NITE P-1363) or Paracoccus AS13 strain (Accession No. NITE P-1364).

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