JPS6480293A - Novel plasmid vector - Google Patents

Novel plasmid vector

Info

Publication number
JPS6480293A
JPS6480293A JP23735487A JP23735487A JPS6480293A JP S6480293 A JPS6480293 A JP S6480293A JP 23735487 A JP23735487 A JP 23735487A JP 23735487 A JP23735487 A JP 23735487A JP S6480293 A JPS6480293 A JP S6480293A
Authority
JP
Japan
Prior art keywords
plasmid vector
fragment
rubisco
atpase
escherichia coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP23735487A
Other languages
Japanese (ja)
Inventor
Taku Ishibashi
Tsutomu Ota
Yumi Shinohara
Masaru Shibata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New Oji Paper Co Ltd
Original Assignee
Oji Paper Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oji Paper Co Ltd filed Critical Oji Paper Co Ltd
Priority to JP23735487A priority Critical patent/JPS6480293A/en
Publication of JPS6480293A publication Critical patent/JPS6480293A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)

Abstract

PURPOSE:To obtain a plasmid vector useful for breeding of arboreous plants, by inserting a chloroplast DNA fragment of back pine containing subunit gene of RuBisCo and H<+>-ATPase into a plasmid derived from Escherichia coli. CONSTITUTION:Chloroplast DNA of black pine is cleaved with a restriction enzyme PstI to provide a Pst-4 fragment (13.7kbp) containing a large subunit of RuBisCo and subunit gene of H<+>-ATPase. The resultant fragment is then inserted into a plasmid derived from Escherichia coli. Thereby the aimed plasmid vector is obtained.
JP23735487A 1987-09-24 1987-09-24 Novel plasmid vector Pending JPS6480293A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP23735487A JPS6480293A (en) 1987-09-24 1987-09-24 Novel plasmid vector

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP23735487A JPS6480293A (en) 1987-09-24 1987-09-24 Novel plasmid vector

Publications (1)

Publication Number Publication Date
JPS6480293A true JPS6480293A (en) 1989-03-27

Family

ID=17014151

Family Applications (1)

Application Number Title Priority Date Filing Date
JP23735487A Pending JPS6480293A (en) 1987-09-24 1987-09-24 Novel plasmid vector

Country Status (1)

Country Link
JP (1) JPS6480293A (en)

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