JPS6478163A - Reagent for immunoassay - Google Patents

Reagent for immunoassay

Info

Publication number
JPS6478163A
JPS6478163A JP23477787A JP23477787A JPS6478163A JP S6478163 A JPS6478163 A JP S6478163A JP 23477787 A JP23477787 A JP 23477787A JP 23477787 A JP23477787 A JP 23477787A JP S6478163 A JPS6478163 A JP S6478163A
Authority
JP
Japan
Prior art keywords
antibodies
contg
group
liposome
antigens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP23477787A
Other languages
Japanese (ja)
Inventor
Yoshio Ishimori
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toshiba Corp
Original Assignee
Toshiba Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toshiba Corp filed Critical Toshiba Corp
Priority to JP23477787A priority Critical patent/JPS6478163A/en
Priority to US07/246,092 priority patent/US5080833A/en
Priority to EP88308701A priority patent/EP0312212B1/en
Priority to DE8888308701T priority patent/DE3872151T2/en
Publication of JPS6478163A publication Critical patent/JPS6478163A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To analyze a sample such as serum contg. a material to be examined precisely with high sensitivity at a low dilution rate by incorporating a free SH group into antibodies or part of the antibodies or antigens to be conjugated. CONSTITUTION:Lipid contg. a halogenated alkyl group is first synthesized by bringing halogenated acetic acid, etc., into reaction with suitable amino group- contg. lipid in the presence of N-hydroxysuccinimide, etc. Phospholipid and glycolipid contg. the suitable halogenated alkyl group and further, a suitable amt. of cholesterol and other lipids are dissolved and mixed together with a solvent in and with said lipid. After the solvent is sucked away, an aq. soln. contg. a labeling material is added to the mixture under shaking to prepare the liquid suspension of liposome. On the other hand, the free SH group is imparted to the antibodies, or part of the antibodies or the antigens by subjecting the same to an enzyme treatment, reduction treatment, etc. The halogenated alkyl group of liposome and the SH group thereof react with each other and the chemical conjugation takes place if these antibodies or part of the antibodies or antigens and the liquid suspension of liposome are mixed under suitable conditions. The desired reagent for analysis is thus obtd.
JP23477787A 1987-09-21 1987-09-21 Reagent for immunoassay Pending JPS6478163A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP23477787A JPS6478163A (en) 1987-09-21 1987-09-21 Reagent for immunoassay
US07/246,092 US5080833A (en) 1987-09-21 1988-09-19 Immobilization of bioactive substance on lipid composition containing modified lipid compound
EP88308701A EP0312212B1 (en) 1987-09-21 1988-09-20 Immobilization of bioactive substance on lipid composition containing modified lipid compound
DE8888308701T DE3872151T2 (en) 1987-09-21 1988-09-20 IMMOBILIZATION OF A BIOACTIVE SUBSTANCE ON A LIPID COMPOSITION, CONTAINING A MODOFIZED LIPID COMPOUND.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP23477787A JPS6478163A (en) 1987-09-21 1987-09-21 Reagent for immunoassay

Publications (1)

Publication Number Publication Date
JPS6478163A true JPS6478163A (en) 1989-03-23

Family

ID=16976207

Family Applications (1)

Application Number Title Priority Date Filing Date
JP23477787A Pending JPS6478163A (en) 1987-09-21 1987-09-21 Reagent for immunoassay

Country Status (1)

Country Link
JP (1) JPS6478163A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992005773A1 (en) * 1990-10-09 1992-04-16 Vestar, Inc. Phospholipid analogue vesicle with a succinimidyl moiety

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992005773A1 (en) * 1990-10-09 1992-04-16 Vestar, Inc. Phospholipid analogue vesicle with a succinimidyl moiety

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