JPS6467182A - Animal syncytium strain excreting nerve growth factor and production of nerve growth factor - Google Patents

Animal syncytium strain excreting nerve growth factor and production of nerve growth factor

Info

Publication number
JPS6467182A
JPS6467182A JP62221851A JP22185187A JPS6467182A JP S6467182 A JPS6467182 A JP S6467182A JP 62221851 A JP62221851 A JP 62221851A JP 22185187 A JP22185187 A JP 22185187A JP S6467182 A JPS6467182 A JP S6467182A
Authority
JP
Japan
Prior art keywords
cells
syncytium
growth factor
nerve growth
strain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP62221851A
Other languages
Japanese (ja)
Inventor
Atsushi Hamada
Masako Taketomi
Hirohisa Tsuda
Naomichi Inui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Tobacco Inc
Original Assignee
Japan Tobacco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc filed Critical Japan Tobacco Inc
Priority to JP62221851A priority Critical patent/JPS6467182A/en
Publication of JPS6467182A publication Critical patent/JPS6467182A/en
Pending legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To efficiently produce NGF useful for the growth, regeneration, maintenance or the like of nerves, by fusing a mouse submandibular cell with a Chinese hamster-originated V79 cell and culturing the obtained syncytium strain in a serum-free medium. CONSTITUTION:Chinese hamster-originated V79 cells are treated with ethyl methanesulfonate to screen a clone resistant against 6-thioguanine. A mouse glandula submandibularis is extirpated, cut in small pieces and subsequently treated with tryptophane/EDTA to disperse its cells, thereby providing mouse submandibular cells. Two kinds of these cells are mixed with each other and treated with PEG 1500 to cause a fusion between the cells. The resulting syncytiums are cultured in a HAT medium and screened to give the objective syncytium having a high NGF (nerve growth factor) content. The syncytium is cultured in a serum-free medium, and the NGF is isolated from the supernatant of the cultured product.
JP62221851A 1987-09-07 1987-09-07 Animal syncytium strain excreting nerve growth factor and production of nerve growth factor Pending JPS6467182A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62221851A JPS6467182A (en) 1987-09-07 1987-09-07 Animal syncytium strain excreting nerve growth factor and production of nerve growth factor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62221851A JPS6467182A (en) 1987-09-07 1987-09-07 Animal syncytium strain excreting nerve growth factor and production of nerve growth factor

Publications (1)

Publication Number Publication Date
JPS6467182A true JPS6467182A (en) 1989-03-13

Family

ID=16773180

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62221851A Pending JPS6467182A (en) 1987-09-07 1987-09-07 Animal syncytium strain excreting nerve growth factor and production of nerve growth factor

Country Status (1)

Country Link
JP (1) JPS6467182A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01211488A (en) * 1988-02-17 1989-08-24 Agency Of Ind Science & Technol External growth factor-undependent cellular strain
CN109628381A (en) * 2019-01-16 2019-04-16 四川大学 A kind of acquisition methods of mice embryonic salivary gland

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01211488A (en) * 1988-02-17 1989-08-24 Agency Of Ind Science & Technol External growth factor-undependent cellular strain
CN109628381A (en) * 2019-01-16 2019-04-16 四川大学 A kind of acquisition methods of mice embryonic salivary gland
CN109628381B (en) * 2019-01-16 2022-04-19 四川大学 Method for obtaining mouse embryo submaxillary gland

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