JPS6467182A - Animal syncytium strain excreting nerve growth factor and production of nerve growth factor - Google Patents
Animal syncytium strain excreting nerve growth factor and production of nerve growth factorInfo
- Publication number
- JPS6467182A JPS6467182A JP62221851A JP22185187A JPS6467182A JP S6467182 A JPS6467182 A JP S6467182A JP 62221851 A JP62221851 A JP 62221851A JP 22185187 A JP22185187 A JP 22185187A JP S6467182 A JPS6467182 A JP S6467182A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- syncytium
- growth factor
- nerve growth
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE:To efficiently produce NGF useful for the growth, regeneration, maintenance or the like of nerves, by fusing a mouse submandibular cell with a Chinese hamster-originated V79 cell and culturing the obtained syncytium strain in a serum-free medium. CONSTITUTION:Chinese hamster-originated V79 cells are treated with ethyl methanesulfonate to screen a clone resistant against 6-thioguanine. A mouse glandula submandibularis is extirpated, cut in small pieces and subsequently treated with tryptophane/EDTA to disperse its cells, thereby providing mouse submandibular cells. Two kinds of these cells are mixed with each other and treated with PEG 1500 to cause a fusion between the cells. The resulting syncytiums are cultured in a HAT medium and screened to give the objective syncytium having a high NGF (nerve growth factor) content. The syncytium is cultured in a serum-free medium, and the NGF is isolated from the supernatant of the cultured product.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62221851A JPS6467182A (en) | 1987-09-07 | 1987-09-07 | Animal syncytium strain excreting nerve growth factor and production of nerve growth factor |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62221851A JPS6467182A (en) | 1987-09-07 | 1987-09-07 | Animal syncytium strain excreting nerve growth factor and production of nerve growth factor |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6467182A true JPS6467182A (en) | 1989-03-13 |
Family
ID=16773180
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62221851A Pending JPS6467182A (en) | 1987-09-07 | 1987-09-07 | Animal syncytium strain excreting nerve growth factor and production of nerve growth factor |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6467182A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01211488A (en) * | 1988-02-17 | 1989-08-24 | Agency Of Ind Science & Technol | External growth factor-undependent cellular strain |
CN109628381A (en) * | 2019-01-16 | 2019-04-16 | 四川大学 | A kind of acquisition methods of mice embryonic salivary gland |
-
1987
- 1987-09-07 JP JP62221851A patent/JPS6467182A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01211488A (en) * | 1988-02-17 | 1989-08-24 | Agency Of Ind Science & Technol | External growth factor-undependent cellular strain |
CN109628381A (en) * | 2019-01-16 | 2019-04-16 | 四川大学 | A kind of acquisition methods of mice embryonic salivary gland |
CN109628381B (en) * | 2019-01-16 | 2022-04-19 | 四川大学 | Method for obtaining mouse embryo submaxillary gland |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES8502476A1 (en) | DNA sequences, recombinant DNA molecules and processes for producing bovine growth hormone-like polypeptides in high yield. | |
UY24668A1 (en) | METHOD TO PRODUCE LINES OF IMMORTALIZED CELLS FOR VIRUS GROWTH | |
GR1000713B (en) | Production of zeaxanthin and zeaxanthin-containing compositions | |
GB9308271D0 (en) | Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method | |
DE69638334D1 (en) | METHOD FOR THE PRODUCTION OF RETROVIRAL PACKAGING CELLINES PRODUCING A RETROVIRAL SUPPLY WITH HIGHLY EFFICIENT TRANSDUCTION RATE | |
ES2108287T3 (en) | METHOD FOR PRODUCING BINDING RESISTANT BINDERS FOR CATALYSTS. | |
ATE329047T1 (en) | STIMULATION OF HOMOLOGUE RECOMBINATION IN PLANT ORGANISMS USING RECOMBINATION-PROMOTING ENZYMES | |
MY112803A (en) | Deproteinized natural rubber latex and its production process | |
GR3026114T3 (en) | Methods for culturing HCV in B- or T-lymphocyte cell lines | |
ES8503689A1 (en) | Teichomycin A2 pure single factors 1, 2, 3, 4 and 5 and method for their production | |
EP0763057A4 (en) | Method for proliferating and differentiating b cells, and uses thereof | |
IL94722A0 (en) | Media for cell growth and their production | |
NO910211L (en) | NEW STRAINS OF PHAFFIA RHODOZYMA WITH HIGH ESTAXANTIN PRODUCTIVITY. | |
AU7387287A (en) | Process for producing biologically active plasminogen activator | |
IL94530A0 (en) | Interleukin-7 receptors,their production and pharmaceutical compositions containing them | |
ATE71661T1 (en) | HYBRID DNA SYNTHESIS OF INSULIN. | |
JPS6467182A (en) | Animal syncytium strain excreting nerve growth factor and production of nerve growth factor | |
ES8701224A1 (en) | Method for production of cytidine and/or deoxycytidine. | |
AR005823A1 (en) | PROCESS TO PRODUCE A CELL FREE ZOSTER VARICELLA VIRUS | |
IL88307A0 (en) | Plants with modified flower colour and methods for their production by genetic engineering | |
HUT35277A (en) | Process for producing proliferating plant cell-formations | |
EP1127104A4 (en) | Method for the massive culture of cells producing recombinant human erythropoietin | |
MY105112A (en) | Probes | |
JPS6427493A (en) | Production of protein containing nonnatural-type amino acid | |
Lechat et al. | Gambierdiscus toxicus: culture and toxin production |