JPS6460382A - Immobilized enzyme membrane, its production and enzyme sensor using said immobilized enzyme membrane - Google Patents

Immobilized enzyme membrane, its production and enzyme sensor using said immobilized enzyme membrane

Info

Publication number
JPS6460382A
JPS6460382A JP62248215A JP24821587A JPS6460382A JP S6460382 A JPS6460382 A JP S6460382A JP 62248215 A JP62248215 A JP 62248215A JP 24821587 A JP24821587 A JP 24821587A JP S6460382 A JPS6460382 A JP S6460382A
Authority
JP
Japan
Prior art keywords
membrane
immobilized
enzyme
silk fibroin
god
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62248215A
Other languages
Japanese (ja)
Other versions
JPH0448437B2 (en
Inventor
Tetsuo Asakura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Terumo Corp
Original Assignee
Terumo Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Terumo Corp filed Critical Terumo Corp
Priority to JP62248215A priority Critical patent/JPS6460382A/en
Priority to US07/176,687 priority patent/US4999295A/en
Publication of JPS6460382A publication Critical patent/JPS6460382A/en
Publication of JPH0448437B2 publication Critical patent/JPH0448437B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
    • C09J4/00—Adhesives based on organic non-macromolecular compounds having at least one polymerisable carbon-to-carbon unsaturated bond ; adhesives, based on monomers of macromolecular compounds of groups C09J183/00 - C09J183/16

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

PURPOSE:To produce an immobilized enzyme membrane having high activity and capable of stably keeping the activity over a long period, by using silk fibroin as the inclusion immobilization material for enzyme taking advantage of the structural characteristics. CONSTITUTION:A solution of regenerated silk fibroin produced by domesticated silkworm is mixed with 0.2% GOD (glucose oxidase) and the mixed solution is cast on an acrylic polymer plate and dried at 4 deg.C. The obtained GOD- immobilized silk fibroin membrane is insolubilized with methanol, fixed to a drawing device, stored in a sealed box at 20 deg.C and 90% relative humidity for 30min and then drawn for 10min at a prescribed draw ratio in an atmosphere having a relative humidity of 90% to stabilize the structure. A glucose sensor can be produced by covering an enzyme electrode with the obtained GOD-immobilized membrane of regenerated silk fibroin produced by domesticated silkworm.
JP62248215A 1987-05-08 1987-10-02 Immobilized enzyme membrane, its production and enzyme sensor using said immobilized enzyme membrane Granted JPS6460382A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP62248215A JPS6460382A (en) 1987-05-08 1987-10-02 Immobilized enzyme membrane, its production and enzyme sensor using said immobilized enzyme membrane
US07/176,687 US4999295A (en) 1987-05-08 1988-04-01 Biocatalyst entrapped in a silk fibroin membrane

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP11068187 1987-05-08
JP62248215A JPS6460382A (en) 1987-05-08 1987-10-02 Immobilized enzyme membrane, its production and enzyme sensor using said immobilized enzyme membrane

Publications (2)

Publication Number Publication Date
JPS6460382A true JPS6460382A (en) 1989-03-07
JPH0448437B2 JPH0448437B2 (en) 1992-08-06

Family

ID=26450253

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62248215A Granted JPS6460382A (en) 1987-05-08 1987-10-02 Immobilized enzyme membrane, its production and enzyme sensor using said immobilized enzyme membrane

Country Status (1)

Country Link
JP (1) JPS6460382A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5328847A (en) * 1990-02-20 1994-07-12 Case George D Thin membrane sensor with biochemical switch

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5328847A (en) * 1990-02-20 1994-07-12 Case George D Thin membrane sensor with biochemical switch

Also Published As

Publication number Publication date
JPH0448437B2 (en) 1992-08-06

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Legal Events

Date Code Title Description
LAPS Cancellation because of no payment of annual fees