JPS6460368A - Cell culture and device therefor - Google Patents

Cell culture and device therefor

Info

Publication number
JPS6460368A
JPS6460368A JP63056329A JP5632988A JPS6460368A JP S6460368 A JPS6460368 A JP S6460368A JP 63056329 A JP63056329 A JP 63056329A JP 5632988 A JP5632988 A JP 5632988A JP S6460368 A JPS6460368 A JP S6460368A
Authority
JP
Japan
Prior art keywords
unit
zones
liquid medium
culture
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP63056329A
Other languages
Japanese (ja)
Other versions
JPH074226B2 (en
Inventor
Takaki Arai
Kimihiko Hamamoto
Michiyuki Tokashiki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Teijin Ltd
Original Assignee
Teijin Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Teijin Ltd filed Critical Teijin Ltd
Priority to JP63056329A priority Critical patent/JPH074226B2/en
Publication of JPS6460368A publication Critical patent/JPS6460368A/en
Publication of JPH074226B2 publication Critical patent/JPH074226B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To obtain a cell culture device of simple structure capable of separating a cell suspension culture solution into live cells and a culture solution, having a cylindrical partition in each of plural culture units and stirring means in a liquid medium with a specific bottom part. CONSTITUTION:A cell culture device of suspension type consists of plural culture units (five in the figure). Cylindrical partitions 2-6 are vertically set approximately at the center of each unit and inner cell circulating zones 7 are divided from outer settling zones 8. The partitions 2-6 are not reached to the bottom of each unit and the unreached parts make connecting parts of both the zones. A liquid medium such as fluorocarbon is introduced to the bottom of each unit and agitating elements 29 are set in the liquid medium. A revolving shaft 10 set through the center of each unit is equipped with agitating elements 9. Discharging means 21-25 for a culture solution are laid at the top of settling zone 8 of each unit and the circulating zones 7 are equipped with oxygen feed pipes 11-15 and oxygen concentration sensors 16-20.
JP63056329A 1987-03-11 1988-03-11 Cell culture device and cell culture method Expired - Fee Related JPH074226B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP63056329A JPH074226B2 (en) 1987-03-11 1988-03-11 Cell culture device and cell culture method

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP5422887 1987-03-11
JP62-54228 1987-03-11
JP63056329A JPH074226B2 (en) 1987-03-11 1988-03-11 Cell culture device and cell culture method

Publications (2)

Publication Number Publication Date
JPS6460368A true JPS6460368A (en) 1989-03-07
JPH074226B2 JPH074226B2 (en) 1995-01-25

Family

ID=26394974

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63056329A Expired - Fee Related JPH074226B2 (en) 1987-03-11 1988-03-11 Cell culture device and cell culture method

Country Status (1)

Country Link
JP (1) JPH074226B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264131A (en) * 1990-09-12 1993-11-23 Hitachi, Ltd. Oxygen-dissolving process and an apparatus for practicing the process

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264131A (en) * 1990-09-12 1993-11-23 Hitachi, Ltd. Oxygen-dissolving process and an apparatus for practicing the process

Also Published As

Publication number Publication date
JPH074226B2 (en) 1995-01-25

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Legal Events

Date Code Title Description
LAPS Cancellation because of no payment of annual fees