JPS6451088A - Manifestation vector - Google Patents

Manifestation vector

Info

Publication number
JPS6451088A
JPS6451088A JP20833387A JP20833387A JPS6451088A JP S6451088 A JPS6451088 A JP S6451088A JP 20833387 A JP20833387 A JP 20833387A JP 20833387 A JP20833387 A JP 20833387A JP S6451088 A JPS6451088 A JP S6451088A
Authority
JP
Japan
Prior art keywords
site
ori
linked
pkk223
plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP20833387A
Other languages
Japanese (ja)
Other versions
JPH0522513B2 (en
Inventor
Kinichiro Miura
Izumi Kumagai
Takeshi Hibino
Satoru Misawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eneos Corp
Original Assignee
Nippon Mining Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Mining Co Ltd filed Critical Nippon Mining Co Ltd
Priority to JP20833387A priority Critical patent/JPS6451088A/en
Publication of JPS6451088A publication Critical patent/JPS6451088A/en
Publication of JPH0522513B2 publication Critical patent/JPH0522513B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:To obtain a manifestation vector for the production of swine adenylate kinase, by carrying out the recombination of a fragment containing a replicator Ori of plasmid PKK223-3 with a fragment containing a replicator Ori of plasmid pUC18. CONSTITUTION:A ptac site obtained by cutting pKK223-3 with restriction enzymes PvuI and NruI is linked, using a T4 ligase, to a site containing Ori obtained by cutting pUC18 with restriction enzymes PvuI and PvuII. cDNA of swine adenylate kinase cloned to PstI site of PUC9 is cut with PstI and linked to SamI site of PKK223-3 to obtain pPAK1, from which pPAK1-2 containing 10 bases between SD sequence and amino terminal group and pPAK3 containing 10 bases between SD and ATG codon just before amino terminal group are obtained. Said plasmid is digested with ECoRI and HindIII and a site containing CDNA of adenylate kinase is linked to the Ori site of PMK2 to obtain PMKAK1-2 and PMKAK3.
JP20833387A 1987-08-24 1987-08-24 Manifestation vector Granted JPS6451088A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP20833387A JPS6451088A (en) 1987-08-24 1987-08-24 Manifestation vector

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP20833387A JPS6451088A (en) 1987-08-24 1987-08-24 Manifestation vector

Publications (2)

Publication Number Publication Date
JPS6451088A true JPS6451088A (en) 1989-02-27
JPH0522513B2 JPH0522513B2 (en) 1993-03-29

Family

ID=16554531

Family Applications (1)

Application Number Title Priority Date Filing Date
JP20833387A Granted JPS6451088A (en) 1987-08-24 1987-08-24 Manifestation vector

Country Status (1)

Country Link
JP (1) JPS6451088A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0412526A2 (en) * 1989-08-10 1991-02-13 Nippon Mining Company Limited Expression vector for hirudin, hirudin fusion protein, transformed microorganism, and method for production of hirudin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0412526A2 (en) * 1989-08-10 1991-02-13 Nippon Mining Company Limited Expression vector for hirudin, hirudin fusion protein, transformed microorganism, and method for production of hirudin

Also Published As

Publication number Publication date
JPH0522513B2 (en) 1993-03-29

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