JPS6449950A - Production of medium for electrophoresis - Google Patents
Production of medium for electrophoresisInfo
- Publication number
- JPS6449950A JPS6449950A JP62207257A JP20725787A JPS6449950A JP S6449950 A JPS6449950 A JP S6449950A JP 62207257 A JP62207257 A JP 62207257A JP 20725787 A JP20725787 A JP 20725787A JP S6449950 A JPS6449950 A JP S6449950A
- Authority
- JP
- Japan
- Prior art keywords
- gel forming
- electrophoresis
- concns
- forming liquids
- polyacrylamide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Peptides Or Proteins (AREA)
Abstract
PURPOSE:To eliminate the disturbance in a DNA fragment sepn. pattern in a production process for a polyacrylamide water gel medium for electrophoresis by substantially equalizing the viscosity of gel forming liquids having two kinds of concns. to be mixed. CONSTITUTION:Two kinds of the gel forming liquids which contain polyacrylamide compds. and crosslinking agents at different concns. and are substantially equalized in the viscosities thereof are prepd. While the relative mixing ratios of these gel forming liquids are gradually changed, the gel forming liquids are mixed under addition of a polymn. initiator thereto in such a manner that the concns. of the above-mentioned polyacrylamide mixtures and crosslinking agents form predetermined gradual changes. This liquid mixture is then applied on a transparent polyethylene terephthalate sheet and the gel forming liquid film is crosslinked and polymerized by projecting the light of a xenon discharge lamp thereon. The medium film for electrophoresis which has a uniform film thickness and the normal one straight DNA migration lane and eliminates the disturbance in the DNA fragment sepn. pattern is thereby obtd.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62207257A JPS6449950A (en) | 1987-08-20 | 1987-08-20 | Production of medium for electrophoresis |
| US07/234,470 US4834854A (en) | 1987-08-20 | 1988-08-19 | Method of making an electrophoresis medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62207257A JPS6449950A (en) | 1987-08-20 | 1987-08-20 | Production of medium for electrophoresis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6449950A true JPS6449950A (en) | 1989-02-27 |
Family
ID=16536800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62207257A Pending JPS6449950A (en) | 1987-08-20 | 1987-08-20 | Production of medium for electrophoresis |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6449950A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009080083A (en) * | 2007-09-27 | 2009-04-16 | Toppan Printing Co Ltd | Laminate, chip, apparatus, and method for electrophoresis-cum-transfer and method for manufacturing this laminate |
-
1987
- 1987-08-20 JP JP62207257A patent/JPS6449950A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009080083A (en) * | 2007-09-27 | 2009-04-16 | Toppan Printing Co Ltd | Laminate, chip, apparatus, and method for electrophoresis-cum-transfer and method for manufacturing this laminate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE3369848D1 (en) | Method for the production of liquid dianhydro-hexitole mixtures from hexitoles, and their use in the production of polymers | |
| DK439583D0 (en) | PROCEDURE FOR POLYMERIZATION OF UNSATURATED ACIDS IN LIQUID OIL PRODUCTS | |
| FR2304565A1 (en) | PROCESS AND APPARATUS FOR THE DOSED DISTRIBUTION OF VERY VISCOUS LIQUIDS, IN PARTICULAR SYRUPS AND CONCENTRATES WITH SPONTANEOUS CONSERVATION | |
| DE3174930D1 (en) | Process and apparatus for mixing solids with liquids | |
| CA1020773A (en) | Method and apparatus for determining the amount of liquid in a liquid/gas flow | |
| FR2138997B1 (en) | ||
| JPS6449950A (en) | Production of medium for electrophoresis | |
| JPS5248823A (en) | Repairing process for tanks | |
| BR7409958A (en) | PERFECT PROCESS FOR THE POLYMERIZATION, IN WATER SUSPENSION, OF ETHYLENICALLY UNSATURATED MONOMERS | |
| ATE124292T1 (en) | LIQUID MIXING APPARATUS FOR PREPARATION OF WATER WITH VARIABLE CARBON ACID CONTENT. | |
| CA1023948A (en) | Method and apparatus for liquid mixing | |
| DE813079C (en) | Process for the production of films from polymeric resins | |
| HU174866B (en) | Process for the selective preparation of liquid hydrocarbons with hydrocracking | |
| AT332115B (en) | PROCESS FOR THE POLYMERIZATION OF ALPHA-OLEFINS | |
| JPS6449951A (en) | Production of medium for electrophoresis | |
| CA944233A (en) | Method of, and apparatus for, heating of liquids | |
| JPS55164811A (en) | Liquid crystal cell forming method | |
| JPS5227079A (en) | Mixing method of liquid crystals | |
| SU1182479A1 (en) | Method of manufacturing sensitometric wedge | |
| JPS6435252A (en) | Manufacture of gradient gel film for electrophoresis | |
| FR2036286A5 (en) | Modification of bituminous coatings with rubber | |
| JPS5214638A (en) | Method for lining polymers | |
| JPS6421345A (en) | Medium film for electrophoresis | |
| JPS6435253A (en) | Manufacture of gradient gel film for electrophoresis | |
| JPS55163741A (en) | Formation of filming membrane |