JPS6447382A - Method for introducing polymeric compound into cell - Google Patents
Method for introducing polymeric compound into cellInfo
- Publication number
- JPS6447382A JPS6447382A JP20606387A JP20606387A JPS6447382A JP S6447382 A JPS6447382 A JP S6447382A JP 20606387 A JP20606387 A JP 20606387A JP 20606387 A JP20606387 A JP 20606387A JP S6447382 A JPS6447382 A JP S6447382A
- Authority
- JP
- Japan
- Prior art keywords
- electrodes
- vessel
- cell suspension
- polymeric compound
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE:To improve the introduction effect of a polymeric compound, by providing a pair of electrodes on opposite walls of a vessel containing a cell suspension liquid and applying high voltage pulse between the electrodes while rotating either the electrodes or the vessel. CONSTITUTION:A pair of electrodes 2a, 2b are positioned on the opposite inner wall surfaces of a cylindrical vessel 1 containing a cell suspension liquid P and are immersed in the suspension. The electrodes are attached to a supporting shaft to enable the rotation of the electrodes 2a, 2b with a rotary mechanism 3. The cylindrical vessel 1 is charged with a cell suspension containing cells to be introduced and an introducing gene such as DNA or RNA. A high-voltage pulse is applied between the electrodes 2a, 2b while rotating the electrodes 2a, 2b or the vessel 1 relative to the cell suspension liquid. The introduction efficiency of a polymeric compound can be improved by this process.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP20606387A JPS6447382A (en) | 1987-08-19 | 1987-08-19 | Method for introducing polymeric compound into cell |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP20606387A JPS6447382A (en) | 1987-08-19 | 1987-08-19 | Method for introducing polymeric compound into cell |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6447382A true JPS6447382A (en) | 1989-02-21 |
Family
ID=16517234
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP20606387A Pending JPS6447382A (en) | 1987-08-19 | 1987-08-19 | Method for introducing polymeric compound into cell |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6447382A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20200102469A (en) * | 2018-03-09 | 2020-08-31 | 프로디자인 소닉스, 인크. | Acoustic treatment for transfection and transduction |
-
1987
- 1987-08-19 JP JP20606387A patent/JPS6447382A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20200102469A (en) * | 2018-03-09 | 2020-08-31 | 프로디자인 소닉스, 인크. | Acoustic treatment for transfection and transduction |
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