JPS644488B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention is a toothpaste containing a preventive agent against gingivitis, alveolar pyorrhea, etc. More specifically, the present invention relates to a toothpaste in which alkyl betaine is further added to a toothpaste containing amylase enzyme. Amylase enzyme is an enzyme that hydrolyzes α-1,4 glucose bonds in polysaccharides that have three or more D-glucose units connected by α-1,4 bonds, and is widely distributed in the animal and plant kingdoms. It is also produced. Amylase enzyme has starch-decomposing and anti-inflammatory effects, and is used as a digestive agent and other pharmaceuticals. In the field of dental materials, it is useful as a drug for preventing, inhibiting the progression of, and treating oral diseases, particularly gingivitis and alveolar pyorrhea. However, when amylase enzyme is added to a commonly used toothpaste, the enzyme loses its activity when stored at relatively high temperatures for a long period of time, making it ineffective as a toothpaste for preventing gingivitis and alveolar pyorrhea. The present inventor has conducted various studies to prevent inactivation and stabilize the amylase enzyme when it is added to toothpaste, and has discovered that the desired purpose can be achieved by adding alkyl betaine. Ta. Therefore, an object of the present invention is to provide a toothpaste that contains amylase enzyme and stabilizes the activity of amylase enzyme for a long period of time in a toothpaste that prevents gingivitis, alveolar pyorrhea, etc. Toothpaste in the present invention refers to toothpaste, moisturizing toothpaste, cream for gum pine surge, etc.
Commonly used abrasives, wetting agents, binders, foaming agents, etc. are used. For example, abrasives include dibasic calcium phosphate for calcium carbonate toothpaste, insoluble sodium metaphosphate, calcium pyrophosphate, silicic anhydride, aluminum hydroxide, etc. Wetting agents include glycerin, propylene glycol, polyethylene glycol, sorbitol, etc. As the agent, methylcellulose, sodium carboxymethylcellulose, sodium alginate, carrageenan, etc. are used, and as the blowing agent, sodium lauryl sulfate, sodium lauroyl sarcosine, polyoxyethylene sorbitan, polyoxyethylene nonyl phenyl ether, etc. are used. The toothpaste of the present invention may also contain various other medicinal agents such as aluminum allantoinate, glycyrrhizinate, sodium chloride, chlorhexidine, azulene, and hinokitiol. The alkyl betaine used in the present invention has the general formula
A compound represented by (1) or (2).

【formula】

[In the formula, R ₁ is an alkyl group or alkenyl group having about 10 to about 18 carbon atoms, R ₂ and R ₃ are alkyl groups having 1 to 3 carbon atoms, m is 1 to 10, and X is a hydrogen atom, -CH ₂ COOM (M is a cation derived from Na, K, NH ₄ or alkanolamine) or -(CH ₂ CH ₂ O) nH (n is 1 to 10), and the above m and n are n+m≦10 is an integer that satisfies the following. ]

Examples of the compound represented by the general formula (1) include dimethyllaurylbetaine, dimethyloleylbetaine, dimethylmyristylbetaine, dimethylstearylbetaine, methylethyllaurylbetaine, methyl n-propyllaurylbetaine, and the like. Further, the compound represented by the general formula (2) is selected from ethoxylated alkyl betaines represented by the following general formula. (Symbols in the formula are as described above) The amount of alkylbetaine added varies depending on the type and each formulation, such as the type and amount of abrasive, binder, wetting agent, etc., but is preferably about 0.1
~about 20% (by weight), more preferably about 1 to about 5%
(weight) amount. Next, the toothpaste obtained according to the present invention has stabilized amylase enzyme and is effective in preventing gingivitis, alveolar pyorrhea, etc. Experimental examples are shown below. (Amylase enzyme analysis method) Disperse 10g of toothpaste in 500ml, and add 1ml of the supernatant.
Add 10ml of 2% starch solution and react at 40° for 6 minutes. After reaction, add 0.01%I ₂ -0.02% to 0.5ml of this solution.
After adding 5 ml of KI-5% HCl solution and allowing the mixture to stand at 20° for 9 minutes, the absorbance at 660 nm was measured using a spectrophotometer to determine the enzyme residual rate in the toothpaste.

[Table] The unit of 1 mg of amylase enzyme used in the present invention is
250 units, and the toothpaste used in the table contains 5mg of amylase enzyme in 1g of toothpaste.

[Table] Table 1 shows the residual rate of amylase enzyme in the case of dimethyl lauryl betaine, and Table 2 shows the residual rate of amylase enzyme in other alkyl betaines measured by the same method after 60 days at 40°C. Examples of the present invention are shown below. Example 1 Calcium pyrophosphate 45.0g Silicic anhydride 2.5 Glycerin 15 Sorbit (70% aqueous solution) 10 Carboxymethylcellulose 1 Fragrance 1 Sodium saccharin 0.1 Methylparaben 0.2 Dimethyllaurylbetaine 2.5 Amylase enzyme 0.5 (125000 units) Dilute to 100g with water. Example 2 Sodium chloride 10.0g Dibasic calcium phosphate 35.5 Glycerin 18.5 Propylene glycol 2.5 Sodium alginate 0.5 Carrageenan 0.6 Alkylolamide 2.0 Fragrance 1.0 Methylparaben 0.2 Sodium saccharin 0.1 Dimethylstearyl betaine 5.0 Amylase enzyme 0.5 (125000 units) Water The total amount is 100g shall be. Example 3 Calcium carbonate 80.0 g Glycerin 5.0 Sodium lauryl sulfate 1.0 Sodium saccharin 0.1 Flavor 1.0 Ethoxylated lauryl betaine 1.0 Amylase enzyme 0.2 (50,000 units) Make the total amount to 100 g with water. Example 4 Aluminum hydroxide 5.0g Dibasic calcium phosphate 80.0 Sodium lauroylsarcosine 0.5 Sodium saccharin 0.1 Flavor 1.0 Dimethylmyristylbetaine 5.0 Amylase enzyme 0.5 (125,000 units) Dilute to 100g with water. Example 5 Calcium pyrophosphate 30.0g Silicic anhydride 1.0 Sodium carboxymethylulose 1.0 Glycerin 10.0 Fragrance 1.0 Sodium saccharin 0.1 Methylparaben 0.1 Dimethyllauryl betaine 20.0 Amylase enzyme 0.5 (125000 units) Dilute to 100g with water. Example 6 Dibasic calcium phosphate 40.0g Anhydrous silicic acid 5.0 Carboxymethylcellulose 0.7 Methylcellulose 0.3 Glycerin 10.0 Polyethylene glycol 400 5.0 Sorbit (70% aqueous solution) 10.0 Sodium lauryl sulfate 1.5 Fragrance 1.0 Methylparaben 0.2 Dimethylmyristylbetaine 0.1 Amylase enzyme 0.5 (125000 units) water The total amount is 100g.

Claims (1)

[Scope of Claims] 1. A toothpaste containing an amylase enzyme, further comprising an alkyl betaine represented by the general formula (1) or (2). [Formula] or [Formula] [In the formula, R ₁ is an alkyl group or alkenyl group having about 10 to about 18 carbon atoms, R ₂ and R ₃ are alkyl groups having 1 to 3 carbon atoms, m is 1 to 10, and X is a hydrogen atom, _-CH2COOM (M is a cation derived from Na, K, _NH4 or _alkanolamine ) or -( _CH2CH2O )nH (n is 1 to 10), and the above m and n is an integer satisfying n+m≦10]