JPS6437300A - Determination of pyruvic acid and reagent therefor - Google Patents
Determination of pyruvic acid and reagent thereforInfo
- Publication number
- JPS6437300A JPS6437300A JP62194076A JP19407687A JPS6437300A JP S6437300 A JPS6437300 A JP S6437300A JP 62194076 A JP62194076 A JP 62194076A JP 19407687 A JP19407687 A JP 19407687A JP S6437300 A JPS6437300 A JP S6437300A
- Authority
- JP
- Japan
- Prior art keywords
- pyruvic acid
- electron acceptor
- acid
- determination
- formazan dye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To easily determine pyruvic acid with a stable dry chemistry reagent, by using a novel enzyme having high thermal stability and capable of converting pyruvic acid into acetylphosphoric acid, CO2 and a reduced-type electron acceptor in the presence of phosphoric acid and an electron acceptor. CONSTITUTION:A specimen containing pyruvic acid is added with (A) phosphoric acid, (B) an electron acceptor (e.g. phenazine methosulfate), (C) a formazan dye (e.g. nitrotetrazolium blue) and (D) a novel pyruvic acid dehydrogenase catalyzing the reaction of formula and stable at pH 6.3 and 50 deg.C for 15min, developing the color of the produced reduced-type electron acceptor with the formazan dye, etc., and the extinction of the reaction system is determined to determine the amount of pyruvic acid in the specimen.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62194076A JPH0677520B2 (en) | 1987-08-03 | 1987-08-03 | Method for measuring pyruvic acid and its reagent |
DE19883820404 DE3820404A1 (en) | 1987-06-15 | 1988-06-15 | Pyruvate dehydrogenase and its use in an analytical method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62194076A JPH0677520B2 (en) | 1987-08-03 | 1987-08-03 | Method for measuring pyruvic acid and its reagent |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6437300A true JPS6437300A (en) | 1989-02-07 |
JPH0677520B2 JPH0677520B2 (en) | 1994-10-05 |
Family
ID=16318558
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62194076A Expired - Fee Related JPH0677520B2 (en) | 1987-06-15 | 1987-08-03 | Method for measuring pyruvic acid and its reagent |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH0677520B2 (en) |
-
1987
- 1987-08-03 JP JP62194076A patent/JPH0677520B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JPH0677520B2 (en) | 1994-10-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
LAPS | Cancellation because of no payment of annual fees |