JPS642576A - Dna fragment - Google Patents

Dna fragment

Info

Publication number
JPS642576A
JPS642576A JP62140586A JP14058687A JPS642576A JP S642576 A JPS642576 A JP S642576A JP 62140586 A JP62140586 A JP 62140586A JP 14058687 A JP14058687 A JP 14058687A JP S642576 A JPS642576 A JP S642576A
Authority
JP
Japan
Prior art keywords
rna
dna
hepatitis
mrna
specific antigen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62140586A
Other languages
Japanese (ja)
Other versions
JP2590885B2 (en
JPH012576A (en
Inventor
Kazunobu Takahashi
Tatsuro Shibui
Michiru Uchida
Rie Kurebayashi
Yutaka Teranishi
Shigetada Nakanishi
Naomi Kitamura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP62140586A priority Critical patent/JP2590885B2/en
Priority to US07/168,357 priority patent/US5032511A/en
Priority to EP88400790A priority patent/EP0293274B1/en
Priority to CN88101758A priority patent/CN1031717A/en
Priority to KR1019880003585A priority patent/KR880011342A/en
Priority to DE8888400790T priority patent/DE3864585D1/en
Publication of JPS642576A publication Critical patent/JPS642576A/en
Publication of JPH012576A publication Critical patent/JPH012576A/en
Application granted granted Critical
Publication of JP2590885B2 publication Critical patent/JP2590885B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE:To provide a DNA fragment containing a base sequence coding a non-A, non-B hepatitis specific antigen protein and useful for the mass- production of a non-A, non-B hepatitis specific antigen protein by a recombinant DNA technique. CONSTITUTION:A liver tissue of a human or chimpanzee affected with non-A, non-B hepatitis is homogenized in an aqueous solution of guanidium thiocyanate and whole RNA is separated as a precipitate by an equilibrium density gradient ultracentrifugation using cesium chloride. The separated whole RNA is purified by the extraction with phenol and the precipitation with ethanol. The RNA is further purified by oligo(dT)-cellulose column chromatography to separate a poly(A)-containing RNA, which is used as a raw material for mRNA. The objective DNA is determined from the mRNA through a cDNA library. The DNA is composed of a sequence of 1,333 bases.
JP62140586A 1987-03-31 1987-06-04 DNA fragment Expired - Lifetime JP2590885B2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP62140586A JP2590885B2 (en) 1987-03-31 1987-06-04 DNA fragment
US07/168,357 US5032511A (en) 1987-03-31 1988-03-15 DNA fragments coding for antigens specific to non-A non-B hepatitis, expression vectors containing said DNA fragments, transformants and process for producing said antigens
CN88101758A CN1031717A (en) 1987-03-31 1988-03-31 Be the dna fragmentation of non-A non-B hepatitis coding for antigens specific, contain the expression vector of this dna fragmentation, its transformant and this antigenic method of production
KR1019880003585A KR880011342A (en) 1987-03-31 1988-03-31 DNA fragments encoding non-A non-B hepatitis specific antigens, expression vectors containing the DNA fragments, transformants, and methods of preparing the antigens
EP88400790A EP0293274B1 (en) 1987-03-31 1988-03-31 Dna molecules encoding non-a, non-b hepatitis antigens, and their use in producing said antigens
DE8888400790T DE3864585D1 (en) 1987-03-31 1988-03-31 DNA MOLECULES, CODING FOR NON-A-NON-B-HEPATITIS ANTIGENS AND THEIR USE FOR THE PRODUCTION OF THESE ANTIGENS.

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP7831387 1987-03-31
JP62-78313 1987-03-31
JP62140586A JP2590885B2 (en) 1987-03-31 1987-06-04 DNA fragment

Publications (3)

Publication Number Publication Date
JPS642576A true JPS642576A (en) 1989-01-06
JPH012576A JPH012576A (en) 1989-01-06
JP2590885B2 JP2590885B2 (en) 1997-03-12

Family

ID=26419397

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62140586A Expired - Lifetime JP2590885B2 (en) 1987-03-31 1987-06-04 DNA fragment

Country Status (1)

Country Link
JP (1) JP2590885B2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007960A1 (en) * 1990-10-24 1992-05-14 Chugai Pharmaceutical Co., Ltd. Method of detecting non-a non-b hepatitis specific gene and reagent composition used therefor
JPH05506773A (en) * 1989-09-15 1993-10-07 国立予防衛生研究所長 Novel HCV isolates
KR100456284B1 (en) * 2002-07-09 2004-11-09 학교법인 인하학원 Rapid method for nucleic acid preparation from microorganism

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05506773A (en) * 1989-09-15 1993-10-07 国立予防衛生研究所長 Novel HCV isolates
WO1992007960A1 (en) * 1990-10-24 1992-05-14 Chugai Pharmaceutical Co., Ltd. Method of detecting non-a non-b hepatitis specific gene and reagent composition used therefor
KR100456284B1 (en) * 2002-07-09 2004-11-09 학교법인 인하학원 Rapid method for nucleic acid preparation from microorganism

Also Published As

Publication number Publication date
JP2590885B2 (en) 1997-03-12

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