JPS6416581A - Frozen animal cell and production thereof - Google Patents
Frozen animal cell and production thereofInfo
- Publication number
- JPS6416581A JPS6416581A JP62171772A JP17177287A JPS6416581A JP S6416581 A JPS6416581 A JP S6416581A JP 62171772 A JP62171772 A JP 62171772A JP 17177287 A JP17177287 A JP 17177287A JP S6416581 A JPS6416581 A JP S6416581A
- Authority
- JP
- Japan
- Prior art keywords
- cell
- culture
- freezing
- substrate
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE:To obtain the titled cell preservable over a long period, without causing the loss of culture tools accompanied by the dissemination of cells after freezing and thawing, by culturing an anchorage-dependent cell on a culture substrate, exchanging the culture medium with a freezing medium and immediately freezing the cell together with the substrate. CONSTITUTION:An anchorage-dependent cell is cultured on a culture substrate, the culture medium is exchanged to a freezing medium and the cell is immediately frozen together with the culture substrate to obtain the objective cell. Preferably, the culture of the cell is carried out until the cell density reaches a confluent state and the surface of the culture substrate is coated with an adhesion- promoting substance (e.g. gelatin) prior to the dissemination of the cell.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62171772A JPS6416581A (en) | 1987-07-09 | 1987-07-09 | Frozen animal cell and production thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62171772A JPS6416581A (en) | 1987-07-09 | 1987-07-09 | Frozen animal cell and production thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6416581A true JPS6416581A (en) | 1989-01-20 |
JPH0577389B2 JPH0577389B2 (en) | 1993-10-26 |
Family
ID=15929394
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62171772A Granted JPS6416581A (en) | 1987-07-09 | 1987-07-09 | Frozen animal cell and production thereof |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6416581A (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0433436U (en) * | 1990-07-17 | 1992-03-18 | ||
JP2008212062A (en) * | 2007-03-05 | 2008-09-18 | Niwa Masami | Method for freeze-preserving cell and method for culturing cell |
WO2009050965A1 (en) * | 2007-10-19 | 2009-04-23 | Japan As Represented By General Director Of Agency Of National Center For Child Health And Development | Support for immobilizing frozen cells, tool for primary hepatocyte culture and method of producing tool for primary hepatocyte culture |
US8742091B2 (en) | 2001-06-20 | 2014-06-03 | Dainippon Sumitomo Pharma Co., Ltd. | Method of promoting nucleic acid transfer |
JP2015500810A (en) * | 2011-11-30 | 2015-01-08 | アドバンスド セル テクノロジー、インコーポレイテッド | Mesenchymal stromal cells and related applications |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20200205399A1 (en) * | 2017-07-31 | 2020-07-02 | Two Cells Co., Ltd. | Composition for cryopreservation, method for producing cryopreserved material, cell preparation, method for producing cell preparation, and kit for cryopreservation |
WO2020067434A1 (en) * | 2018-09-27 | 2020-04-02 | テルモ株式会社 | Method for cryopreserving cells |
-
1987
- 1987-07-09 JP JP62171772A patent/JPS6416581A/en active Granted
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0433436U (en) * | 1990-07-17 | 1992-03-18 | ||
US8742091B2 (en) | 2001-06-20 | 2014-06-03 | Dainippon Sumitomo Pharma Co., Ltd. | Method of promoting nucleic acid transfer |
JP2008212062A (en) * | 2007-03-05 | 2008-09-18 | Niwa Masami | Method for freeze-preserving cell and method for culturing cell |
WO2009050965A1 (en) * | 2007-10-19 | 2009-04-23 | Japan As Represented By General Director Of Agency Of National Center For Child Health And Development | Support for immobilizing frozen cells, tool for primary hepatocyte culture and method of producing tool for primary hepatocyte culture |
JP2015500810A (en) * | 2011-11-30 | 2015-01-08 | アドバンスド セル テクノロジー、インコーポレイテッド | Mesenchymal stromal cells and related applications |
Also Published As
Publication number | Publication date |
---|---|
JPH0577389B2 (en) | 1993-10-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |