JPS6410987A - Production of lysozyme - Google Patents

Production of lysozyme

Info

Publication number
JPS6410987A
JPS6410987A JP15749487A JP15749487A JPS6410987A JP S6410987 A JPS6410987 A JP S6410987A JP 15749487 A JP15749487 A JP 15749487A JP 15749487 A JP15749487 A JP 15749487A JP S6410987 A JPS6410987 A JP S6410987A
Authority
JP
Japan
Prior art keywords
dna
human lysozyme
lysozyme
prepared
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP15749487A
Other languages
Japanese (ja)
Inventor
Masakazu Kikuchi
Koji Yoshimura
Kazuo Nakahama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda Pharmaceutical Co Ltd
Original Assignee
Takeda Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd
Publication of JPS6410987A publication Critical patent/JPS6410987A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To obtain a large amount of an active human lysozyme by recombinant DNA method, by using DNA prepared by bonding DNA to code a specific signal peptide of to 5' end of DNA to code a human lysozyme. CONSTITUTION:DNA which has leucine at fourth of amino acid sequence of signal peptide of glair lysozyme replaced with phenylalanine, isoleucine at sixth with leucine and valine at eighth with alanine, (i) selects a codon having high use frequency in yeast, (ii) uses sequence of a yeast PGK gene in the upper stream of ATG to raise an amount of manifestation and (iii) determines nucleotide sequence to enable construction of hybrid signal and codes a signal sequence shown by the formula is prepared. The DNA is bonded to DNA which is chemically synthesized based on the amino acid sequence of human lysozyme and codes a human lysozyme having TaqI site in the vicinity of N end. A cell transformed by using the prepared DNA is cultivated and human lysozyme is used and accumulated in the culture mixture.
JP15749487A 1986-06-30 1987-06-26 Production of lysozyme Pending JPS6410987A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15180986 1986-06-30

Publications (1)

Publication Number Publication Date
JPS6410987A true JPS6410987A (en) 1989-01-13

Family

ID=15526781

Family Applications (2)

Application Number Title Priority Date Filing Date
JP15749387A Pending JPS6410986A (en) 1986-06-30 1987-06-26 Production of lysozyme
JP15749487A Pending JPS6410987A (en) 1986-06-30 1987-06-26 Production of lysozyme

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP15749387A Pending JPS6410986A (en) 1986-06-30 1987-06-26 Production of lysozyme

Country Status (1)

Country Link
JP (2) JPS6410986A (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0388834A (en) * 1989-05-31 1991-04-15 Japan Synthetic Rubber Co Ltd Thermoplastic polymer composition
JPH0735325Y2 (en) * 1990-02-23 1995-08-09 ヒロセ電機株式会社 Printed circuit board connector lock structure
FR2662304B1 (en) * 1990-05-21 1992-07-24 France Telecom METHOD OF MANUFACTURING AN INTEGRATED STRUCTURE OF LIGHT SENSOR GUIDE IN SEMICONDUCTOR MATERIAL.

Also Published As

Publication number Publication date
JPS6410986A (en) 1989-01-13

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