JPS6385347A - Sampling device - Google Patents
Sampling deviceInfo
- Publication number
- JPS6385347A JPS6385347A JP61232149A JP23214986A JPS6385347A JP S6385347 A JPS6385347 A JP S6385347A JP 61232149 A JP61232149 A JP 61232149A JP 23214986 A JP23214986 A JP 23214986A JP S6385347 A JPS6385347 A JP S6385347A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- sampling unit
- pipe
- electrolyte
- capillary tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005070 sampling Methods 0.000 title claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 3
- 239000011358 absorbing material Substances 0.000 claims description 4
- 239000000523 sample Substances 0.000 abstract description 16
- 238000001962 electrophoresis Methods 0.000 abstract description 10
- 239000003792 electrolyte Substances 0.000 abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 7
- 239000012488 sample solution Substances 0.000 abstract description 2
- 230000005012 migration Effects 0.000 abstract 3
- 238000013508 migration Methods 0.000 abstract 3
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 6
- 239000008151 electrolyte solution Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
Description
【発明の詳細な説明】
（イ）産業上の利用分野
本発明は、電気泳動におけるサンプリングユニットに関
する。DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention relates to a sampling unit in electrophoresis.
（ロ）従来技術
従来電気泳動において、サンプルを定量的に泳動用ゲル
に添加するためには、先ずマイクロシリンジを使ってサ
ンプル定量分取し、これをろ紙に保持させたシミ解液を
満たした溝に注入したシして添加していた。(b) Conventional technology In conventional electrophoresis, in order to quantitatively add a sample to a running gel, a microsyringe is first used to quantitatively separate the sample, which is then filled with a stain-resolving solution held on filter paper. It was added by injecting it into the groove.
ｅ勺発明が解決しようとする問題点
従来法では、サンプＡ／ｌ−ゲルに添加する際にろ紙に
保持させたシ、電解液で満ちた溝に注入しているので、
いたずらにサンプリング領域を拡大したシ、サンプルが
断続的にゲルに入って行くためにストリークがでるなど
の問題があった。Problems that the invention aims to solve In the conventional method, when adding electrolyte to sump A/L-gel, it was held on filter paper and injected into a groove filled with electrolyte.
There were problems such as unnecessarily expanding the sampling area and streaks appearing as the sample entered the gel intermittently.
本考案は、サンプル定量分取と泳動用ゲルの限定領域へ
のアプライ機能を併せもった構造のサンプリングユニッ
トを提供することを目的とする。The purpose of the present invention is to provide a sampling unit having a structure that combines the functions of quantitatively separating a sample and applying a gel for electrophoresis to a limited area.
に）問題点を解決するための手段
発明は、管内にピストンロッドを収容したサンプリング
ユニットにおいて、管材質を多孔質吸水体で形成すると
ともに、管のロッド側に対向する一端にキャピラリを嵌
合させたことを特徴とする。2) Means for Solving the Problems The invention is a sampling unit in which a piston rod is housed in a tube, in which the tube material is made of a porous water absorbing material, and a capillary is fitted into one end of the tube opposite to the rod side. It is characterized by:
（ホ））作用
本発明は１毛管現象又は界面張力を利用してサップ／ｌ
’を定量保持し、泳動用ゲルと電解液の界面にサンプ／
Ｌ／ヲ添加する。(e)) Effect The present invention utilizes capillarity or interfacial tension to
' is held in a quantitative manner and sampled at the interface between the electrophoresis gel and the electrolyte.
Add L/wo.
（へ）実施例 本発明の実施例を図面に基づいて説明する。(f) Example Embodiments of the present invention will be described based on the drawings.
第１図（ａ）は１本考案のサンプリングユニットＳを用
いてサンプリングするときの図、第１図（ｂ）は泳動用
ゲルへサンプルを添加するときの図である。FIG. 1(a) is a diagram when sampling is performed using the sampling unit S of the present invention, and FIG. 1(b) is a diagram when a sample is added to the electrophoresis gel.
第１図（ａ）においてｌがキャピラリで０−リング３を
介して多孔質吸水体（例えば、セルボール、サンファイ
ン入Ｑ）の管４の内側に嵌合されている。なお６が樹脂
性のピストンロッドである。またキャピラリ１の長さは
、泳動に必要なサンプル量を保持するように調節されて
いる。In FIG. 1(a), a capillary 1 is fitted through an O-ring 3 to the inside of a tube 4 of a porous water absorbing material (for example, Cell Ball, Sunfine-containing Q). Note that 6 is a resin piston rod. Further, the length of the capillary 1 is adjusted so as to hold the amount of sample necessary for electrophoresis.
この構造において、サンプリングユニットＳを第１図の
ようにサンプル溶液７中に浸漬すると毛管現象によりサ
ンプルはキャピラリ１に保持される。２が保持され友サ
ンプ／Ｌ’を示す。サンプ／Ｉ／ｌ−保持した後、泳動
用ゲルに添加する。In this structure, when the sampling unit S is immersed in the sample solution 7 as shown in FIG. 1, the sample is held in the capillary 1 by capillary action. 2 is retained to indicate friend sump/L'. Sample/I/l - After retention, add to running gel.
この時の状態図が第１図（ｂ）である。The state diagram at this time is shown in FIG. 1(b).
第】図（ｂ）で８が泳動用ゲル、９が電解液、１０が電
極である。図のようにサンプリングユニットＳｆ、電解
液９に浸漬すると多孔質吸水体４全通して電解液が５の
部分に浸入し、サンプ／Ｌ／２は泳動用ゲ／Ｌ／８と電
解液９の境界に存在し、しかも電場の中におかれること
になる。この状態で！ＦＭ１０に電位をかけるとサンプ
ル２は泳動ゲ／Ｌ’８中を泳動して行く。In Figure (b), 8 is a gel for electrophoresis, 9 is an electrolytic solution, and 10 is an electrode. As shown in the figure, when the sampling unit Sf is immersed in the electrolytic solution 9, the electrolytic solution passes through the entire porous water absorbent body 4 and enters the part 5, and the sump/L/2 is connected to the electrophoretic gel/L/8 and the electrolytic solution 9. It exists at the boundary and is placed in an electric field. In this condition! When a potential is applied to the FM10, the sample 2 migrates through the electrophoresis gel/L'8.
（ト）効果
本発明によればサンプルを分取したままアプライでき、
しかも、サンプルがキャピラリで囲まれているので電解
液中でも拡散しない。(g) Effects According to the present invention, the sample can be applied as it is separated,
Moreover, since the sample is surrounded by a capillary, it does not diffuse even in the electrolyte.
第１図（ａ）が本発明のサンプリングユニットを用いて
サンプリングするときの図、第１図（ｂ）がサンプ／Ｉ
／ヲ泳動用ゲ〃に添加する図である。
ｌ・・・キャピヲ９ ４ ・・・多孔質吸水体第
（シ）
１日FIG. 1(a) is a diagram of sampling using the sampling unit of the present invention, and FIG. 1(b) is a diagram of sampling using the sampling unit of the present invention.
/ is a diagram showing how to add it to the electrophoresis gel. l... Capio 9 4... Porous water absorbing material No. 1 (shi) 1 day
Claims (1)
ットにおいて、管材質を多孔質吸水体で形成するととも
に、管のロッド側に対向する一端にキャピラリを嵌合さ
せたことを特徴とするサンプリングユニット。1. A sampling unit in which a piston rod is accommodated in a tube, characterized in that the tube material is made of a porous water-absorbing material, and a capillary is fitted to one end of the tube facing the rod side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61232149A JPH0758281B2 (en) | 1986-09-29 | 1986-09-29 | Sampling unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61232149A JPH0758281B2 (en) | 1986-09-29 | 1986-09-29 | Sampling unit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6385347A true JPS6385347A (en) | 1988-04-15 |
| JPH0758281B2 JPH0758281B2 (en) | 1995-06-21 |
Family
ID=16934761
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61232149A Expired - Fee Related JPH0758281B2 (en) | 1986-09-29 | 1986-09-29 | Sampling unit |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0758281B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998009159A1 (en) * | 1996-08-29 | 1998-03-05 | The Regents Of The University Of California | Micro injector sample delivery system for charged molecules |
-
1986
- 1986-09-29 JP JP61232149A patent/JPH0758281B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998009159A1 (en) * | 1996-08-29 | 1998-03-05 | The Regents Of The University Of California | Micro injector sample delivery system for charged molecules |
| US5980713A (en) * | 1996-08-29 | 1999-11-09 | The Regents Of The University Of California | Micro injector sample delivery system for charged molecules |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0758281B2 (en) | 1995-06-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |