JPS6371186A - Cultivation method - Google Patents

Cultivation method

Info

Publication number
JPS6371186A
JPS6371186A JP21578486A JP21578486A JPS6371186A JP S6371186 A JPS6371186 A JP S6371186A JP 21578486 A JP21578486 A JP 21578486A JP 21578486 A JP21578486 A JP 21578486A JP S6371186 A JPS6371186 A JP S6371186A
Authority
JP
Japan
Prior art keywords
culture
culture medium
hollow fibers
hollow
passed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP21578486A
Other languages
Japanese (ja)
Other versions
JPH0439314B2 (en
Inventor
Kenji Kato
加藤 建児
Seiji Nomura
野村 聖次
Masakatsu Fujimoto
藤本 正勝
Kunihiko Too
東尾 邦彦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to JP21578486A priority Critical patent/JPS6371186A/en
Publication of JPS6371186A publication Critical patent/JPS6371186A/en
Publication of JPH0439314B2 publication Critical patent/JPH0439314B2/ja
Granted legal-status Critical Current

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  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PURPOSE:To efficiently cultivate a cultivation object assuming a specific form, by introducing the cultivation object into hollow pipes consisting of a membrane, impermeable to the cultivation object and permeable to a solute in a culture medium, hermetically sealing the above-mentioned pipes and entrapping the cultivation object. CONSTITUTION:A process system is kept in a germ-free state by using a thermal sterilization method with formaldehyde and steam and spawns are passed from a spawn tank through hollow fibers 5. A fresh culture medium is passed from a culture medium tank through the shell side of a hollow fiber module 1 to carry out cultivation to a given concentration. Fixed bolts 15 and head parts at both ends are aseptically removed from the module 1 and mounted in an inoculating pot 8. The hollow fibers 5 with a fixing plate 13 are passed from above through a fiber guide 6 and pushed down. A heating wire 7 as a heating source is moved in a direction crossing the hollow fibers 5 to cut and thermally fuse the hollow fibers 5 to a given length. The resultant entrapped material is then dropped into the inoculating pot 8 containing a previously introduced culture medium to keep microorganisms or cells in the entrapped body from inactivation.

Description

【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、培養方法に係り、特に糸状菌、放線菌、植物
組織等の培養の進行により繊維状あるいはひも状等のよ
うに特異な形態をとる培養対象を培養するのに好適な培
養方法に関するものである。
[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a culturing method, and in particular, the present invention relates to a method for culturing filamentous fungi, actinomycetes, plant tissues, etc., which, as a result of the progress of culturing, develop unique shapes such as fibrous or string-like shapes. The present invention relates to a culture method suitable for culturing a culture target that takes .

〔従来の技術〕[Conventional technology]

従来、糸状菌、放線菌、植物組織等の培養は、一時的な
微生物培養と同様に、通気および機械的な攪拌あるいは
通気と通気による攪拌によって行われている。
Conventionally, culturing of filamentous fungi, actinomycetes, plant tissue, etc., has been carried out by aeration and mechanical stirring, or by aeration and agitation by aeration, as in the case of temporary microbial culture.

しかし、この種の培養対象は、培養の進行によって繊維
状あるいはひも状等のように特異な形態をとるため、各
センサーの汚れによる応答遅れや液中の見掛は粘度の増
大による攪拌所要動力の増加等のハード的問題、更には
、機械的な攪拌による培養対象のせん断およびそれに伴
なう活性低下等のプロセス的問題を有している。
However, as the culture progresses, this type of culture target takes on a unique shape such as fibrous or string-like shapes, so there is a delay in response due to dirt on each sensor, and the power required for stirring due to the apparent increase in viscosity in the liquid. In addition, there are hardware problems such as an increase in the number of cells, as well as process problems such as shearing of the culture target due to mechanical stirring and an accompanying decrease in activity.

そこで、このような問題に対して、例えば、特開昭54
−117085号公報に記載のような、  ・溶餐液中
に微生物の生育場所として多孔質物質を存在させる技術
が提案されている。
Therefore, in order to solve such problems, for example,
A technique has been proposed, as described in Japanese Patent No. 117085: - A technique is proposed in which a porous substance is made to exist in a solution as a breeding place for microorganisms.

〔発明が解決しようとする問題点〕[Problem that the invention seeks to solve]

上記従来技術では、多孔質物質の孔径によって培養対象
が限定されてしまうこと、培養液中の菌体濃度を上げに
くいこと、菌体が多孔質物質よりはくHして培養液中に
浮遊するのを防止できないこと等の問題を有しており、
上記のような培養対象について依然として高効率な培養
を行い得ないといった問題がある。
In the above-mentioned conventional technology, the culture target is limited by the pore size of the porous material, it is difficult to increase the concentration of bacterial cells in the culture solution, and the bacterial cells are expelled from the porous material and float in the culture solution. There are problems such as the inability to prevent
There is still a problem that highly efficient culture of the above-mentioned culture targets cannot be performed.

本発明の目的は、培養の進行により繊維状あるいはひも
状等のように特異な形態をとる培養対象について高効率
の培養を行うことができる培養方法を提供することにあ
る。
An object of the present invention is to provide a culture method that can perform highly efficient culture of a culture target that takes a unique shape such as a fibrous or string shape as the culture progresses.

〔問題点を解決するための手段〕[Means for solving problems]

上記目的は、培養方法を、培養対象を該培養対象が透過
不可、かつ、培地中の溶質が透過可能な膜からなる中空
管内に導入する工程と、該中空管の端部を封止して前記
培養対象を包括化する工程とを有する方法とすることに
より、達成される。
The above purpose is to provide a culture method including a step of introducing a culture target into a hollow tube made of a membrane through which the culture target cannot pass through and a solute in the medium can pass through, and sealing the end of the hollow tube. This is achieved by providing a method that includes the step of enclosing the culture target.

〔作  用〕[For production]

培養対象が透過不可、かつ、培地中の溶質が透過可能な
膜からなる中空管内に培養対象を導入し。
A culture target is introduced into a hollow tube made of a membrane that is impermeable to the culture target and permeable to solutes in the medium.

その後、中空管の端部な封止して培養対象を包括化する
。これにより、培養対象が、培養の進行により繊維状あ
るいはひも状等のように特異な形態をとる培養対象であ
っても、見かけ上、通常形態の培養対象と同等に扱え培
養が行える。
Thereafter, the end of the hollow tube is sealed to enclose the culture target. As a result, even if the culture target takes on a unique shape such as a fibrous or string-like shape as the culture progresses, it can be treated and cultured in the same way as a culture target with a normal shape.

〔実 施 例〕〔Example〕

以下、本発明の一実施例を第1図〜第3図により、具体
的に説明するが、本発明はこれにより、何ら限定される
ものではない。
Hereinafter, one embodiment of the present invention will be described in detail with reference to FIGS. 1 to 3, but the present invention is not limited thereto in any way.

第1図に、本発明による前培養段階の一実施例の概略を
、第2図に、前培養から本培養への移行操作を、また第
3図に本実施例に使用するホローファイバーモジュール
の構成概略なおのおの示す。
Fig. 1 shows an outline of one embodiment of the pre-culture stage according to the present invention, Fig. 2 shows the transition operation from pre-culture to main culture, and Fig. 3 shows the hollow fiber module used in this example. The outline of each structure is shown below.

本装置は、主として、ホローフ1イバーモジュール!、
培地槽29種菌膜3.植菌ボット8より構成されており
、第1図に示すフローにて、ホローファイバー5内で目
的の微生物または細胞を前培養後、第2図に示す操作に
よって、ホローファイバー5を適当な長さに切断し、微
生物または細胞の包括体として、植菌ポット8内に保持
する。なお、ホローファイバー5は、目的の微生物また
は細胞が透過不可で、かつ、培地中の溶質が透過可能な
膜、例えば、セルロース膜で形成されている。
This device is mainly used for the Hollow 1 Eve module! ,
Culture medium tank 29 seeds bacterial membrane 3. It consists of an inoculation bot 8, and after pre-cultivating the desired microorganisms or cells in the hollow fiber 5 according to the flow shown in Fig. 1, the hollow fiber 5 is grown to an appropriate length by the operation shown in Fig. 2. The microorganisms or cells are then cut into pieces and held in an inoculation pot 8 as a microorganism or cell enclosing body. The hollow fiber 5 is formed of a membrane, such as a cellulose membrane, which is impermeable to the target microorganism or cell and permeable to solutes in the culture medium.

以下、本実施例による操作の概略を説明する。An outline of the operation according to this embodiment will be explained below.

前培養時には、第1図に示すフローにて培養が行われる
。すなわち、まず種菌槽3を経由して、ホルムアルデヒ
ド(HCHO)を、ホローファイバーモジュール1に通
液し、これを循環させることによって、モジュールl内
を滅菌し、さらに、モジュール以外のプロセス系な蒸気
で加熱滅菌して、プロセス系を全て無菌状態とする。次
に、種菌(SEED)を種菌槽3から、ホローファイバ
ー5内に通液する。ファイバー5内が満液状態になった
ら、出口側のバルブを閉とし、バッファーで配管および
種菌槽3内を洗浄して、全ての種菌が、ホローファイバ
ー5内に保持されるようにする。この状態で、培地槽2
より、新鮮培地をホローファイバーモジュールlのシェ
ル側に通液し、ファイバー5内の微生物または細胞に、
培地を供給し、この微生物または細胞が、所定の濃度に
達するまで培養する。
At the time of pre-culturing, culturing is performed according to the flow shown in FIG. That is, first, formaldehyde (HCHO) is passed through the hollow fiber module 1 via the seed tank 3, and by circulating this, the inside of the module 1 is sterilized, and further, the inside of the module 1 is sterilized with process steam other than the module. Heat sterilize to make all process systems sterile. Next, the seed culture (SEED) is passed from the seed culture tank 3 into the hollow fiber 5. When the inside of the fiber 5 is filled with liquid, the valve on the outlet side is closed, and the piping and the inside of the seed tank 3 are washed with a buffer so that all the seed bacteria are retained in the hollow fiber 5. In this state, medium tank 2
Then, a fresh medium is passed through the shell side of the hollow fiber module 1, and the microorganisms or cells inside the fiber 5 are
A medium is supplied and the microorganisms or cells are cultured until they reach a predetermined concentration.

前培養終了後は、第2図に示す操作にて、前培養した微
生物または細胞を包括化する。すなわち。
After the preculture is completed, the precultured microorganisms or cells are incorporated by the operation shown in FIG. Namely.

第3図に示すような構造を有するホローファイバーモジ
ュールlから、無菌の雰囲気下にて、固定ボルト15お
よび両端のヘッド部を取外して第2図に示すように、植
菌ポット8に装着する。上部より、ホローファイバー5
を、ファイバー固定板13ごとファイバーガイド6を通
して押下げ、第2図に示す発熱線7にクロム線、白金線
等)をホローファイバー5を横切る方向に移動させて所
定の長さに切断、熱溶着し、得られた包括体を植菌ポッ
ト8内に落′し込む。植菌ポット8内には、あらかじめ
、調製法の培地またはバッファーを入れておき、得られ
た包括体内の微生物または細胞が失活しないよう保持す
る。
The fixing bolts 15 and the head portions at both ends are removed from the hollow fiber module l having the structure shown in FIG. 3 in a sterile atmosphere, and the module is attached to the inoculation pot 8 as shown in FIG. 2. From the top, hollow fiber 5
is pushed down together with the fiber fixing plate 13 through the fiber guide 6, and a chrome wire, platinum wire, etc.) is moved in a direction across the hollow fiber 5 to the heating wire 7 shown in FIG. Then, the obtained enclosing body is dropped into the inoculation pot 8. The culture medium or buffer of the preparation method is placed in advance in the inoculation pot 8, and the microorganisms or cells in the obtained inclusion body are maintained so as not to be inactivated.

以上1本実施例では目的とする微生物又は細胞なホロー
ファイバーで包括し、該包括体を単位として培養するよ
うにしているため、通常形態をとる他の培養対象と見か
け上、同等に扱うことができ通常の培養装置によっても
高効率な培養を行うことができる。
In this example, the target microorganism or cell is enclosed in a hollow fiber, and the enclosing body is cultured as a unit, so that it can be treated in the same manner as other culture objects that take normal forms. Highly efficient culture can be performed even with ordinary culture equipment.

また、本実施例によれば、微生物または細胞を膜の内部
で、新鮮培地な与えながら、前培養し、そのまま、同一
の条件で本培養を行うことができるため、微生物または
細胞の活性を低下させることな(、培養を継続すること
ができる。
Furthermore, according to this example, microorganisms or cells can be precultured inside the membrane while being fed with fresh medium, and then main culture can be carried out under the same conditions, thereby reducing the activity of microorganisms or cells. (The culture can be continued.

なお、上記実施例の他に、前培養後の培養対象なホロー
ファイバー内に導入した後に上記のようにして封止し包
括性を得るようにしてもよい。
In addition to the above-mentioned embodiments, the fiber may be introduced into the hollow fiber to be cultured after pre-culture and then sealed as described above to obtain comprehensiveness.

〔発明の効果〕〔Effect of the invention〕

本発明によれば、培養対象が、培養の進行により繊維状
あるいはひも状等のように特異な形態をとる培養対象で
あっても、通常形態ンとる他の培養対象と見かけ上、同
等に扱え培養できるので、高効率な培養を行うことがで
きるという効果がある。
According to the present invention, even if a culture target takes on a unique shape such as a fibrous or string-like shape as the culture progresses, it can be treated in the same way as other culture targets that take a normal shape. Since it can be cultured, it has the effect of being able to perform highly efficient culture.

【図面の簡単な説明】[Brief explanation of the drawing]

第1図は、本発明の一実施例の前培養装置のフロー図、
第2図は、第1図のホローファイバーモジュールからの
包括体化装置の外観図、第3図は、第1図のホローファ
イバーモジュールの縦断面口である。 1・・・・・・ホローファイバーモジュール、2・・・
・・・培tl[,3・・・・−・種lli槽、5・・・
・・・ホローファイバー、′;f2図
FIG. 1 is a flow diagram of a preculture device according to an embodiment of the present invention;
FIG. 2 is an external view of the enclosing device from the hollow fiber module of FIG. 1, and FIG. 3 is a vertical cross-sectional view of the hollow fiber module of FIG. 1. 1...Hollow fiber module, 2...
...Culture tl[,3...--Seed lli tank,5...
...Hollow fiber, ′; f2 diagram

Claims (1)

【特許請求の範囲】 1、培養対象を該培養対象が透過不可、かつ、培地中の
溶質が透過可能な膜からなる中空管内に導入する工程と
、該中空管の端部を封止して前記培養対象を包括化する
工程とを有することを特徴とする培養方法。 2、前記中空管の端部を熱溶着して封止する特許請求の
範囲第1項記載の培養方法。
[Scope of Claims] 1. A step of introducing a culture target into a hollow tube made of a membrane through which the culture target cannot permeate and a solute in the medium can permeate, and sealing the end of the hollow tube. A culture method comprising the step of enclosing the culture target. 2. The culture method according to claim 1, wherein the end of the hollow tube is sealed by heat welding.
JP21578486A 1986-09-16 1986-09-16 Cultivation method Granted JPS6371186A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP21578486A JPS6371186A (en) 1986-09-16 1986-09-16 Cultivation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP21578486A JPS6371186A (en) 1986-09-16 1986-09-16 Cultivation method

Publications (2)

Publication Number Publication Date
JPS6371186A true JPS6371186A (en) 1988-03-31
JPH0439314B2 JPH0439314B2 (en) 1992-06-29

Family

ID=16678173

Family Applications (1)

Application Number Title Priority Date Filing Date
JP21578486A Granted JPS6371186A (en) 1986-09-16 1986-09-16 Cultivation method

Country Status (1)

Country Link
JP (1) JPS6371186A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004081157A (en) * 2002-08-28 2004-03-18 Electric Power Dev Co Ltd Method for culturing photosynthetic microorganism and device for the same
DE102007010866A1 (en) 2007-03-02 2008-09-04 Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e.V. -Hans-Knöll-Institut- Device for immersing microcultivation and investigation of growth, morphology, and metabolism of microorganisms or cells, comprises tension-free unidimensional element and nutrient for growth of microorganisms or cells
US20130199977A1 (en) * 2010-10-15 2013-08-08 Snu R & Db Foundation Container with biofilm formation-inhibiting microorganisms immobilized therein and membrane water treatment apparatus using the same
US20160030890A1 (en) * 2010-10-15 2016-02-04 Snu R&Db Foundation Container with biofilm formation-inhibiting microorganisms immobilized therein and membrane water treatment apparatus using the same

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004081157A (en) * 2002-08-28 2004-03-18 Electric Power Dev Co Ltd Method for culturing photosynthetic microorganism and device for the same
DE102007010866A1 (en) 2007-03-02 2008-09-04 Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e.V. -Hans-Knöll-Institut- Device for immersing microcultivation and investigation of growth, morphology, and metabolism of microorganisms or cells, comprises tension-free unidimensional element and nutrient for growth of microorganisms or cells
US20130199977A1 (en) * 2010-10-15 2013-08-08 Snu R & Db Foundation Container with biofilm formation-inhibiting microorganisms immobilized therein and membrane water treatment apparatus using the same
JP2013540443A (en) * 2010-10-15 2013-11-07 ソウル大学校産学協力団 Biofilm formation-inhibiting microorganism immobilization container and separation membrane water treatment apparatus using the same
US20160030890A1 (en) * 2010-10-15 2016-02-04 Snu R&Db Foundation Container with biofilm formation-inhibiting microorganisms immobilized therein and membrane water treatment apparatus using the same

Also Published As

Publication number Publication date
JPH0439314B2 (en) 1992-06-29

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