JPS635702B2 - - Google Patents
Info
- Publication number
- JPS635702B2 JPS635702B2 JP57172627A JP17262782A JPS635702B2 JP S635702 B2 JPS635702 B2 JP S635702B2 JP 57172627 A JP57172627 A JP 57172627A JP 17262782 A JP17262782 A JP 17262782A JP S635702 B2 JPS635702 B2 JP S635702B2
- Authority
- JP
- Japan
- Prior art keywords
- voltage
- detector
- circuit
- signal
- input signal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000002218 isotachophoresis Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 238000001962 electrophoresis Methods 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 150000002500 ions Chemical class 0.000 description 15
- 239000000523 sample Substances 0.000 description 8
- 238000002955 isolation Methods 0.000 description 5
- 230000037230 mobility Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 230000010355 oscillation Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 230000003189 isokinetic effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Indication And Recording Devices For Special Purposes And Tariff Metering Devices (AREA)
Description
【発明の詳細な説明】
この発明は等速電気泳動装置に関する。さらに
詳しくは、この発明は信号電圧が低いリーデイン
グイオンやそれに近似の易動度をもつ試料イオン
の検出の分解能を向上することを目的とするもの
で、検出器からの信号電圧を、入力信号反転回路
で反転して加算減算回路に入力し、この回路内で
電圧−周波数変換器（Ｖ／Ｆコンバータ）の最大
入力電圧に反転した信号電圧を加算してリーデイ
ング電圧およびその近傍における試料イオンの電
圧を高精度で処理することができる等速電気泳動
装置に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an isotachophoresis device. More specifically, the present invention aims to improve the detection resolution of leading ions with low signal voltages and sample ions with similar mobilities, by converting the signal voltage from the detector into an inverted input signal. The inverted signal voltage is inverted in the circuit and input to the addition/subtraction circuit, and the inverted signal voltage is added to the maximum input voltage of the voltage-frequency converter (V/F converter) in this circuit to obtain the reading voltage and the voltage of the sample ion in the vicinity. The present invention relates to an isotachophoresis device that can process with high precision.
従来の等速電気泳動装置例えば第１図に示す１
ａでは、泳動管４ａの途中に設置された検出器６
ａからの電位勾配入力信号は、インピーダンス変
換器７ａを介して電圧−周波数コンバータ１０ａ
に入力され、同コンバータ１０ａからの出力をア
イソレーシヨン回路１１ａに入力したのちカウン
ター１２ａでパルスを測定して分析されていた。
この方法でターミナルイオン及び通常ターミナル
イオンの５〜20倍であるリーデイングイオンやそ
れに近似の易動度を持つ試料イオンの周波数につ
いて考える。電圧−周波数コンバータ１０ａの発
振周波数をアイソレーシヨン回路１１ａなどの関
係で約100KHz／10Vとし、ターミナルイオン及
びリーデイングイオンの信号電圧をそれぞれ10V
と0.5Vとしたとき、リーデイング電圧での周波
数fLはfL＝5KHzとなり、カウンターで10ｍsecご
とに読むことのできるパルス数は50ケと少なくな
るため、リーデイング電圧及びその近傍における
試料イオンの電圧のように低レベルの信号を高精
度で処理することができず、分解能が低いという
欠点があつた。 A conventional isotachophoresis apparatus, for example 1 shown in FIG.
In a, a detector 6 installed in the middle of the electrophoresis tube 4a
The potential gradient input signal from a is passed through an impedance converter 7a to a voltage-frequency converter 10a.
After inputting the output from the converter 10a to the isolation circuit 11a, the pulses were measured and analyzed by the counter 12a.
In this method, we consider the frequencies of terminal ions, leading ions whose mobility is 5 to 20 times that of normal terminal ions, and sample ions whose mobility is similar to that of terminal ions. The oscillation frequency of the voltage-frequency converter 10a is set to approximately 100KHz/10V due to the isolation circuit 11a, etc., and the signal voltages of the terminal ion and leading ion are each 10V.
and 0.5V, the frequency f L at the reading voltage is f L = 5KHz, and the number of pulses that can be read every 10 msec by the counter is as small as 50, so the reading voltage and the voltage of the sample ion in the vicinity It has the disadvantage of being unable to process low-level signals with high precision, such as low resolution.
この発明は上記欠点を解消することができる等
速電気泳動装置を提供するものである。 The present invention provides an isotachophoresis device that can eliminate the above-mentioned drawbacks.
この発明の実施例を第２図以下について詳述す
る。なお、これによつてこの発明が限定を受ける
ものではない。 An embodiment of the present invention will be described in detail with reference to FIG. 2 and subsequent figures. Note that this invention is not limited by this.
第２図において、等速電気泳動装置１は、リー
デイング電極槽２と、ターミナル電極槽と、両電
極槽２，３を接続する試料注入室５及び検出器６
を備えた泳動管４と、検出器６からの検出信号の
インピーダンス変換器７、入力信号反転回路８、
加算減算回路９、電圧−周波数コンバータ１０、
アイソレーシヨン回路１１、カウンター１２から
なる信号処理回路１３と、両電極槽２，３の各電
極に電圧を与える泳動電源１４とから主として構
成されている。 In FIG. 2, the isotachophoresis apparatus 1 includes a leading electrode tank 2, a terminal electrode tank, a sample injection chamber 5 that connects both electrode tanks 2 and 3, and a detector 6.
an impedance converter 7 for the detection signal from the detector 6, an input signal inversion circuit 8,
addition/subtraction circuit 9, voltage-frequency converter 10,
It mainly consists of a signal processing circuit 13 consisting of an isolation circuit 11 and a counter 12, and a migration power supply 14 that applies voltage to each electrode of both electrode tanks 2 and 3.
信号回路１３のインピーダンス変換器７は検出
器６からのPGセル入力信号のインピーダンスを
変換する役目を果すもので、インピーダンス変換
器７からの出力電圧は、入力信号反転回路８に入
力されて出力電圧の正負の符号を反転される。入
力信号反転回路８で電圧値の符号を反転された出
力は、加算減算回路９に入力されて電圧−周波数
の最大入力電圧の値と加算される。リーデイング
イオンやそれに近似の易動度をもつ信号電圧が
0.5Vである場合について説明すれば、0.5Vの入
力信号は入力信号反転回路８で−0.5Vに反転さ
れ、加算減算回路９で最大入力電圧である10Vに
加算されて10V−0.5V＝9.5Vとなる。 The impedance converter 7 of the signal circuit 13 serves to convert the impedance of the PG cell input signal from the detector 6, and the output voltage from the impedance converter 7 is input to the input signal inverting circuit 8 to be converted into an output voltage. The sign of is reversed. The output whose sign of the voltage value has been inverted by the input signal inversion circuit 8 is input to the addition/subtraction circuit 9 and is added to the value of the maximum input voltage of voltage-frequency. The signal voltage with the leading ion or similar mobility is
To explain the case of 0.5V, the 0.5V input signal is inverted to -0.5V by the input signal inversion circuit 8, and added to the maximum input voltage of 10V by the addition/subtraction circuit 9, resulting in 10V - 0.5V = 9.5. It becomes V.
加算減算回路９からの出力信号は、この出力信
号に比例する周波数のパルスを発生する電圧−周
波数コンバータ１０に入力され、同コンバータ１
０からの出力信号はアイソレーシヨン回路１１に
入力したのちカウンター１２でパルスを測定して
分析される。この場合、分析を検出器６からの高
速信号に追随して真の値に近いところを読み取れ
るようにするには、カウンター１２でカウントす
る時間間隔を短くする必要があるが、この時間間
隔の短縮は、インピーダンス変換器７と電圧−周
波数コンバータ１０との間に入力信号反転回路８
と加算減算回路９とを直列に挿入したことにより
リーデイング電圧値近傍の値を持つ試料イオンを
高分解能で検出することができる。すなわち、リ
ーデイングイオンやそれに近似の易動度をもつ信
号電圧が0.5Vであるとき、加算減算回路９の出
力電圧は先に述べたように9.5Vとなり、電圧−
周波数コンバータ１０の発振周波数が約100K
Hz／10Vのとき、リーデイング電圧0.5Vは、電圧
−周波数コンバータ１０の入力信号としては、
9.5Vとなる。そのまま0.5Vを電圧−周波数コン
バータに入力したときは5KHzとなりカウンター
１２で10ｍsecごとにパルスを測定するとすれば、
そのパルス数はそれぞれ50ケであるが、9.5Vの
場合は950ケとなる。このパルス数の著しい増加
のため、リーデイング電圧及びその近傍における
試料イオンの電圧による信号を分解能を低下させ
ることなく高精度で処理することができる。 The output signal from the addition/subtraction circuit 9 is input to a voltage-frequency converter 10 that generates a pulse with a frequency proportional to this output signal.
The output signal from 0 is input to an isolation circuit 11, and then a counter 12 measures and analyzes the pulses. In this case, in order to enable the analysis to follow the high-speed signal from the detector 6 and read a value close to the true value, it is necessary to shorten the time interval at which the counter 12 counts. is an input signal inversion circuit 8 between the impedance converter 7 and the voltage-frequency converter 10.
By inserting the addition/subtraction circuit 9 in series, sample ions having values near the reading voltage value can be detected with high resolution. That is, when the signal voltage of a leading ion or a signal having a mobility similar to it is 0.5V, the output voltage of the addition/subtraction circuit 9 is 9.5V as described above, and the voltage -
The oscillation frequency of frequency converter 10 is approximately 100K
When the reading voltage is 0.5V at Hz/10V, the input signal of the voltage-frequency converter 10 is:
It becomes 9.5V. If 0.5V is directly input to the voltage-frequency converter, it will be 5KHz, and if the counter 12 measures pulses every 10msec, then
The number of pulses is 50 each, but in the case of 9.5V, it is 950. Due to this significant increase in the number of pulses, signals due to the reading voltage and the sample ion voltage in the vicinity thereof can be processed with high precision without reducing resolution.
第４図はこの発明の要部の具体的な回路構成の
一例を示したものであるが、検出器６からの入力
信号が負の場合も、負の信号を正の信号に変換す
る反転回路を別に設ければ、入力信号が正の場合
と同様に取扱うことができる。 FIG. 4 shows an example of a specific circuit configuration of the main part of the present invention, and even when the input signal from the detector 6 is negative, an inverting circuit is used to convert the negative signal into a positive signal. If it is provided separately, it can be handled in the same way as when the input signal is positive.
なお、上記実施例は検出器６を電位勾配検出器
としてその信号について説明したが、検出器６は
電位勾配検出器に限定されるものではなく熱検出
器または導電率検出器であつてもよい。 Note that in the above embodiment, the signal was explained using the detector 6 as a potential gradient detector, but the detector 6 is not limited to a potential gradient detector, and may be a heat detector or a conductivity detector. .
以上説明したことから明らかなように、この発
明は検出器からの入力信号を入力信号反転回路で
信号の符号を変え、加算減算回路で電圧−周波数
の最大入力電圧の値に加算することによつて、リ
ーデイング電圧での周波数を大きくし、カウンタ
ーでの同一測定時間に対するパルスを多くしたも
のであるから、PGセルからの高速の信号に追随
できるだけでなく、リーデイング電解液や試料液
からの小信号をも十分に検出して分解能を低下さ
せることなく高精度で処理することができるすぐ
れた作用効果を奏する。 As is clear from the above explanation, the present invention changes the sign of the input signal from the detector using an input signal inversion circuit, and adds it to the maximum input voltage value of voltage-frequency using an addition/subtraction circuit. Therefore, since the frequency of the reading voltage is increased and the number of pulses is increased for the same measurement time on the counter, it is possible to not only follow high-speed signals from the PG cell, but also to track small signals from the reading electrolyte and sample solution. It has an excellent effect of being able to sufficiently detect and process with high precision without reducing resolution.
第１図は従来の一実施例を示す機能説明図、第
２図はこの発明の一実施例を示す機能説明図、第
３図は検出器として電位勾配検出器を用いた場合
の電気泳動分析図、第４図はこの発明の要部を具
体的に示した回路構成説明図である。
１……等速電気泳動装置、２……リーデイング
電極槽、３……ターミナル電極槽、４……泳動
管、５……試料注入室、６……検出器、７……イ
ンピーダンス変換器、８……入力信号反転回路、
９……加算減算回路、１０……電圧−周波数コン
バータ、１１……アイソレーシヨン回路、１２…
…カウンター。
Fig. 1 is a functional explanatory diagram showing one conventional embodiment, Fig. 2 is a functional explanatory diagram showing one embodiment of the present invention, and Fig. 3 is an electrophoretic analysis when a potential gradient detector is used as a detector. FIG. 4 is an explanatory diagram of a circuit configuration specifically showing the main part of the present invention. DESCRIPTION OF SYMBOLS 1... Isokinetic electrophoresis device, 2... Reading electrode tank, 3... Terminal electrode tank, 4... Electrophoresis tube, 5... Sample injection chamber, 6... Detector, 7... Impedance converter, 8 ...Input signal inversion circuit,
9... Addition/subtraction circuit, 10... Voltage-frequency converter, 11... Isolation circuit, 12...
…counter.
Claims (1)
と、両電極槽を接続し試料注入室及び検出器を設
置した泳動管と、検出器からの検出信号を処理す
る信号処理回路とを備え、更にこの信号処理回路
が、検出器からの検出入力信号を反転する入力信
号反転回路とこの反転回路からの出力信号を電圧
−周波数の最大入力電圧の値に加算する加算減算
回路と、電圧−周波数コンバータと、カウンター
とをこの順で備えたことを特徴とする等速電気泳
動装置。1 Equipped with a terminal electrode tank, a leading electrode tank, an electrophoresis tube that connects both electrode tanks and is equipped with a sample injection chamber and a detector, and a signal processing circuit that processes the detection signal from the detector, and further includes a signal processing circuit that processes the detection signal from the detector. The circuit includes an input signal inversion circuit that inverts a detected input signal from the detector, an addition/subtraction circuit that adds the output signal from the inversion circuit to a maximum input voltage value of voltage-frequency, a voltage-frequency converter, and a counter. An isotachophoresis apparatus characterized by comprising: and in this order.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57172627A JPS5961769A (en) | 1982-09-30 | 1982-09-30 | Uniform velocity electrophoretic apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57172627A JPS5961769A (en) | 1982-09-30 | 1982-09-30 | Uniform velocity electrophoretic apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5961769A JPS5961769A (en) | 1984-04-09 |
| JPS635702B2 true JPS635702B2 (en) | 1988-02-04 |
Family
ID=15945380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57172627A Granted JPS5961769A (en) | 1982-09-30 | 1982-09-30 | Uniform velocity electrophoretic apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5961769A (en) |
-
1982
- 1982-09-30 JP JP57172627A patent/JPS5961769A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5961769A (en) | 1984-04-09 |
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