JPS6347661A - Seal jig of analytical slide - Google Patents

Abstract

PURPOSE:To reduce the variation of a measured value, by forming at least a part of the surface of a seal jig with a non-adsorbable polymer. CONSTITUTION:When an analytical slide carrier 10 is turned inside out, the bottom part (the upper side surface shown by a drawing) and one side part are opened as shown by the drawing. The almost central part of the inside surface of the carrier 10 becomes the surface part corresponding to the liquid specimen imparted surface of a multiplayer analytical element. A non-adsorbable polymer has low surface energy and hardly generates adsorption and absorption physically and chemically with respect to the reactive gas generated from an analytical slide. Therefore, by providing the non-adsorbable polymer so as to cover the surface part as shown by an oblique line part 11, the adsorption of the reactive gas generated in the analytical element by a seal jig can be reduced and the variation in a measured value can be reduced.

Description

[Detailed description of the invention] [Field of invention] The present invention relates to a closure for analytical slides.

More specifically, the present invention provides a seal having a function of reducing evaporation of a liquid sample from a multilayer analytical element during an incubation step during a clinical chemistry test operation in which a specific component in a liquid sample is quantitatively analyzed using an analytical slide. This relates to the improvement of ingredients.

[Background of the Invention] In current medical care, importance is placed on clinical chemistry tests that perform quantitative analysis of specific components in biological fluids in order to perform accurate diagnosis and appropriate treatment. A spectroscopic measurement method can be cited as a measurement principle frequently used in this clinical chemistry test.

Spectroscopic measurement methods are based on specific components to be measured in a sample (
This method uses the principle of colorimetric photometry of the absorbance or change in absorbance of the analyte (analyte) or the product of the analyte produced as a result of a chemical reaction including an enzymatic reaction. If the analyte or its product itself does not develop color or change color, an appropriate color-forming reagent that couples with the chemical reaction of the analyte to form a color can also be used.

In conventional clinical chemistry tests, analysis using the above measurement principle has been carried out by an analysis method called a wet method. For example, the wet method is carried out by the following operations, taking measurement using an enzyme reaction as an example.

A liquid sample such as plasma containing an analyte (or its diluted solution) and an enzyme solution are placed in a cell, thoroughly mixed, and then placed in an incubator to cause an enzyme reaction. The incubator in this wet method consists of a bath filled with water and a heating source to maintain the bath at a predetermined temperature, for example, about 37°C. This is done by leaving it for 10 minutes. After this incubation, light of a predetermined wavelength is emitted from one side of the cell, for example near ultraviolet region (wavelength=19
0-400nm) or visible region light (wavelength: 400-400nm)
800 nm), the light transmitted through the cell and the solution is photoelectrically converted by a photodetector, and the analyte is quantitatively analyzed from its absorbance.

However, this method using cells or test tubes requires a large amount of liquid sample, is cumbersome to handle, is difficult to perform simple and quick measurements, and measures a large number of test liquids continuously. There are problems such as the fact that it is difficult to do so. In order to deal with such problems, a dry analysis method using a multilayer analytical element has been proposed as an alternative to the above-mentioned wet method, and this dry method has already been put into practical use in some areas.

A multilayer analytical element generally consists of a transparent sheet-like plastic support with a reaction layer containing a reagent that reacts directly or indirectly with an analyte to cause color formation, discoloration, etc. Various types of multilayer analysis elements based on this basic configuration are already known.

Multilayer analytical elements are typically used in the form of analytical slides housed in thin plastic frames with openings on both the top and bottom sides. Examples of such analysis slides include Utility Model Application No. 56-142454 and Japanese Patent Application Publication No. 57
-63452, an integrated multilayer analytical element in which a transparent support, a reagent layer, and a porous development layer are sequentially arranged, and an aperture for colorimetric photometry in the center (in the shape of a circular hole). It consists of a lower frame with an opening (part) formed in the center and an upper frame with an opening formed in the center for applying a liquid sample, and an integrated multilayer analysis element is housed between the lower frame and the upper frame, An example of this is a structure in which two are welded and joined.

In general, an analysis operation of a liquid sample using an analysis slide as described above involves depositing the liquid sample onto a multilayer analytical element through an opening in the upper frame, or spreading the liquid sample within the analytical element at a temperature of 37°C, for example. After incubating for 1 minute to allow sufficient color development, light is projected onto the color development part of the analytical element through the hole in the lower frame, and the light reflected from the reaction layer is measured colorimetrically to perform quantitative analysis of specific components. It is implemented using a method.

Analytical operations for liquid samples using analytical slides.

This can be done automatically by using an analytical device that enables accurate analytical measurements and simplification of measurement operations. Such analyzers include a spotting site for spotting a liquid sample onto an analytical slide, and a spotting site for heating and holding the analytical slide on which the liquid sample has been spotted at a constant temperature to allow the color reaction of the analyte to proceed. It is equipped with a device (incubator) to carry out the analysis, and a photometric device to optically detect the color reaction within the analysis slide. This incubator is a device for incubating one analysis slide at an appropriate temperature and time depending on the reaction system to be used.

As mentioned above, the analytical slide has an opening for depositing a liquid sample on the multilayer analytical element, but there is a problem that the water in the liquid sample deposited on the analytical element evaporates, especially during the incubation process. There is. In addition, when using a reaction system in which a reactive gas such as ammonia or carbon dioxide is generated by the reaction between an analyte and a reagent within one analytical element, and a color reaction is caused using this reactive gas as a reaction component. In this case, a part of the generated gas passes through the porous spreading layer and is released to the outside through the above-mentioned openings, and does not contribute to the desired color reaction, so the obtained analytical value is lower than the true value. There is a problem with the value.

Therefore, the purpose of reducing the evaporation of the liquid sample, making the heating of the analysis slide more efficient and uniformizing the heating conditions, and depending on the reaction system used, the release of the generated gas to the outside. For various purposes, including the purpose of preventing analyte slides, the incubation step may be carried out with the analyte slides contained and sealed in an analyte slide carrier. The analysis slide carrier is a carrier that moves together with the analysis slide while accommodating and sealing the analysis slide during the incubation process. An example of an analytical slide carrier is one in the form of a housing that is opened at the part facing the lower surface (support side surface) of a multilayer analytical element, as described in Japanese Patent Application Laid-Open No. 58-21566. Can be mentioned.

The present inventor investigated a quantitative analysis method for components in a liquid sample using an analytical slide and an analytical slide carrier, and found that a reactive gas such as ammonia is generated in the incubation step, and the gas causes a color reaction. It has been found that the use of known analytical slide carriers for the sealing of analytical slides utilizing reaction systems that involve catalytic reactions causes serious problems that affect measurement accuracy.

That is, a significant portion of the reactive gas generated by the chemical reaction of the analyte within the analytical element housed in the analytical slide does not contribute to the color reaction and is transferred to the internal surface of the analytical slide carrier, particularly of the analytical element. It was found that there was a problem with adsorption on the internal surface facing the liquid sample spotting surface. After the analytical slide stored therein has been used for a cycle of analytical operations, the analytical slide carrier is used to eject the analytical slide, then newly accommodate and seal an unused analytical slide, and then store the analytical slide. It is used repeatedly to seal during the incubation process. Therefore, as mentioned above, in the first incubation step, the inner surface of the analysis slide carrier adsorbs the reactive gas, reducing the color reaction.
Therefore, the measured value tends to be lower than the true value, whereas in the second and subsequent incubation steps, the consumption of reactive gas due to adsorption to the inner surface of the carrier is significantly reduced, or the reactive gas is Either the gas no longer adsorbs to the inner surface of the carrier, or the reactive gas adsorbed to the inner surface of the carrier desorbs and contributes to the color reaction of the analytical slide, making the measured value higher than the true value. There is a tendency for various errors to occur.

That is, even with known analytical slide closures such as analytical slide carriers, variations in the measurements obtained cannot be sufficiently prevented.

OBJECTS OF THE INVENTION A first object of the present invention is to provide an improved analytical slide closure.

A second object of the present invention is to provide a sealing device for an analytical slide that has a reduced adsorption force for reactive gases generated by chemical reactions of analytes in multilayer analytical elements housed in the analytical slide. It is in.

A third object of the invention is to provide a closure for analysis slides that allows for a reduction in fluctuations in measured values.

[Summary of the Invention] The invention is based on applying a liquid sample to a multilayer analytical element in the form of an analytical slide housed in a frame having openings on both upper and lower sides, and incubating the liquid sample to generate liquid within the multilayer analytical element. It has the function of reducing evaporation of the liquid sample from the multilayer analysis element in the above-mentioned incubation step of the liquid sample analysis method consisting of detecting the reaction of the liquid sample, and substantially covers the liquid sample application surface of the multilayer analysis element. a sealing device disposed adjacent to an analytical slide, characterized in that at least a portion of the surface of the sealing device facing the liquid sample application surface of the multilayer analytical element is formed of a non-adsorbent polymer. on the sealant of the analysis slide.

[Effects of the Invention] The non-adsorptive polymer used in the sealing tool for the analytical slide of the present invention has a small surface energy and is unlikely to physically adsorb or absorb reactive gases generated from the analytical slide. Furthermore, since the non-adsorptive polymer is a chemically stable substance, it does not chemically adsorb or absorb the above-mentioned reactive gas. Therefore, the sealing device for the analysis slide of the present invention hardly adsorbs the reactive gas generated by the chemical reaction of the analyte in the multilayer analysis element housed in the analysis slide, so that the above-mentioned measured value It is extremely effective in reducing fluctuations in

In addition, the non-adsorptive polymer also has the effect of improving the slippage between the analytical slide and the sealing device for the analytical slide, which is advantageous in analytical operations, particularly in automatic analysis using an analytical device. Furthermore, since the non-adsorptive polymer is a chemically stable substance as described above, it does not have an adverse effect on the reagents contained in the multilayer analytical element and its layer structure.

Therefore, the sealing device for analysis slides of the present invention poses no problem when used for analysis slides that do not generate reactive gases, and is advantageous in terms of analysis operations.

Furthermore, since non-adsorbent polymers are widely used materials, the present invention can be easily implemented.

DETAILED DESCRIPTION OF THE INVENTION The analytical slide closures of the present invention specifically encompass a variety of configurations and functions.

An example is one in which the closure moves together with the analysis slide during the incubation step and is in the form of a housing that is open at the part facing the lower surface of the multilayer analysis element. Specific examples include the "carrier" used in the incubator described in JP-A No. 58-21566;
The combination of the "holding body" and the upper cover 1 used in the incubator described in JP-A No. 8-21567, and the "slide holding member" used in the analyzer described in JP-A-53-81292, etc. Another example is one in which one closure is configured to accommodate and seal one analysis slide so that each closure is moved within the analyzer.

In addition, there is also one in which the sealing device moves together with the analysis slide during the incubation step, is in the form of a sheet, and is adapted to seal the top opening of the frame that accommodates the analysis slide. A specific example is the moisture prevention evaporation cover 1 used in the liquid sample chemical analysis cartridge described in Japanese Utility Model Publication No. 10620/1983, which is provided on the top surface of the analysis slide so that it can be opened and closed. I can list things. As stated in the above publication, some of these closures include:
A closure having one end or one side of the frame that is openable and closably fixed to one end or one side of the frame containing the analysis slide, and a side wall having grooves on at least two opposing sides, along the grooves. A closure is included that can be slid in and out.

Furthermore, as another example of a sealing device that moves together with the analysis slide during the incubation process, there is a "sealing guide" used in a constant temperature holding device for chemical analysis described in Japanese Utility Model Application Publication No. 57-647. Some belts, such as the belt 1, are configured in a belt shape and have a sealing effect.

Further, as the sealing device, a sealing device that moves relative to the analysis slide during the incubation step,
For example, the "conveying member" used in the analysis device described in JP-A No. 56-77746 has a disc-shaped structure, and has an insertion part for a plurality of analysis slides around it. Some allow analytical operations to be performed simultaneously.

Another example of a sealing device that does not move relative to the analysis slide is the upper part of the storage chamber that can accommodate the analysis slide from the side, as described in Japanese Patent Application No. 61-122990. An example of this is the vertically movable slide presser plate 1 attached to the analyzer.In this analyzer, a number of horizontally fixed storage chambers also serve as an incubator, and the photometry section is placed relative to the storage chamber. It is designed to measure light by moving the slide plate.
The portion corresponding to the opening portion of the slide frame may form a shallow recess.

That is, the sealing device of the present invention applies a liquid sample to a multilayer analytical element in the form of an analytical slide housed in a frame having openings on both the upper and lower sides, and incubates the liquid sample so that the liquid sample is transferred into the multilayer analytical element. It has a function of reducing evaporation of the liquid sample from the multilayer analysis element in the above-mentioned incubation step of the liquid sample analysis method consisting of detecting the reaction that has occurred, and substantially covers the liquid sample application surface of the multilayer analysis element. There are no other specific limitations as long as the closure is placed adjacent to the analysis slide in a similar manner. Note that, as is clear from the above description, "sealing" in the present invention does not necessarily mean that the analysis slide is completely isolated from the outside air, but rather refers to the evaporation of the liquid sample from the multilayer analysis element during the incubation process. It also includes a degree of sealing that can be reduced compared to evaporation when the slide remains in place.

However, the closure to which the present invention is applied is in the form of a housing that is opened at the portion facing the lower surface of the multilayer analytical element, as shown in the attached FIGS. 1, 2, and 3. Preferably, the carrier is a carrier for storing and transporting analysis slides.

The closure device of the present invention can achieve the objects of the present invention if at least a portion of the closure surface facing the liquid sample application surface of the multilayer analysis element is formed of a non-adsorptive polymer. Alternatively, it is preferable that substantially the entire surface of the sealing device corresponding to the liquid sample applying surface of the multilayer analytical element be formed of a non-adsorbent polymer.

There are no particular limitations on the non-adsorptive polymer that can be used in the present invention, but for example, polyolefin polymers such as polyethylene and polypropylene, and polyester polymers such as polyethylene terephthalate, butylene terephthalate, and polyethylene/polybutylene terephthalate are particularly advantageously used. can. These non-adsorptive polymers can be manufactured by referring to materials such as the Polymer Materials Handbook (Polymer Science Society of Japan), but products with various properties are already commercially available from many manufacturing companies.
These commercially available products can be used.

Various methods can be used to form the surface portion of the analytical slide closure with the non-adsorbent polymer.

For example, manufacturing a closure by molding a non-adsorbent polymer, applying a non-adsorbent polymer solution to a metal closure;
Applying a melt of the non-adsorbent polymer to the closure, drying and applying a layer of non-adsorbent polymer, cooling and applying a layer of non-adsorbent polymer, sealing with a tape or film made of the non-adsorbent polymer. Methods such as coating the surface of the fastener with a layer containing a non-adsorbable polymer are used.

An example of applying a non-adsorbent polymer to the closure of the present invention will now be described using the analytical slide carrier shown in FIGS. 1, 2 and 3 as an example.

In Figures 1 and 2, the analysis slide carrier (
The slide (made of metal) is indicated by 10 and is configured to receive an analysis slide 20 as shown in FIG. , FIG. 2 shows the analysis slide carrier shown in FIG. 1 turned over so that the interior is visible. The carrier is open at the bottom (lower side in FIG. 1 and upper side in FIG. 2) and on one side. This configuration is
Streamlined incubation process and uniform heat application,
This is effective in increasing the efficiency of loading and unloading analysis slides into the carrier. In addition, an opening 12 (aperture for checking the analysis slide) provided in a part of the lid part 13 of the carrier (the upper surface in FIG. 1 and the lower surface in FIG. It has a function that facilitates the detection of 2o.

In the analytical slide carrier shown in FIGS. 1 and 2, approximately the center of the inner surface of the carrier in FIG. Therefore, it is effective to attach a non-adsorptive polymer to cover the corresponding surface area, as shown at 11 (shaded area) in FIG. 2.

Furthermore, as in the analytical slide carrier shown in FIG. 3, the non-adsorbent polymer can also be applied to other surfaces of the inner surface of the carrier, which is also a preferred embodiment of the present invention. The carrier 30, analysis slide confirmation opening 32, and lid part 33 in FIG. 3 are the same as those in FIG. 1 and 2, respectively.
Carrier lO1 analysis slide confirmation opening 12 in the figure
and the lid portion 13, respectively. In the analytical slide carrier shown in FIG. 3, a non-adsorbent polymer is applied such that the entire inner surface of the carrier is coated with the non-adsorbent polymer, as shown at 31 (shaded area) in FIG. It is attached to.

Furthermore, it is also possible to apply a non-adsorbent polymer to the entire surface of the carrier. In this case, in addition to the effects of the present invention described above, the carriers also have the effect of improving the slippage between them, which is further advantageous in terms of analysis operations.

Alternatively, the entire carrier can be formed from a non-adsorbent polymer.

Incidentally, as in the above-mentioned known example, the analysis slide sealing device can be provided in an analysis device used when performing automatic analysis using an analysis slide. In this case, the position where the non-adsorbent polymer is attached to the sealing device may be considered in the same manner. That is, by applying a single layer of non-adsorptive polymer to at least a portion of the surface of the sealing device that corresponds to the liquid sample application position of the multilayer analytical element within the analytical slide, preferably to the entire corresponding surface portion, the analytical element It is possible to reduce adsorption of reactive gas generated within the sealing device to the sealing device.

There are several examples of multilayer analysis elements that generate reactive gases during analysis operations, but specifically, ``Ammonia or ammonia-generating substrate analysis element'' described in JP-A-58-77661 is a Body type multilayer analysis material J and “integrated analysis element” described in Japanese Patent Publication No. 5B-19062
can be mentioned. Especially when the generated gas is ammonia, i.e., when it is an analyte, ammonia, or an ammonia-producing substrate, ammonia is a gas that is easily adsorbed, and in general, ammonia is an important analyte in the analysis of biological fluids. 1 from the point of
The analytical slide closure of the present invention is particularly effective. The term "ammonia-producing substrate" refers to a compound or compound group that produces ammonia by itself by reacting with a specific reagent, or that produces ammonia via multiple reaction systems. Specific examples thereof include urea and creatinine.

Next, the present invention will be specifically explained with reference to Examples, but it goes without saying that the present invention is limited to these examples. The analysis slide used in Example 1 contained an integrated multilayer analysis element prepared as described below.

[Creation of integrated multilayer analytical element for urea nitrogen analysis] Transparent polyethylene terephthalate (PET) film (thickness 1
80 JLm) on top of which a color-forming indicator layer (dry layer thickness 10
pm) was applied and dried. Next, a water-repellent membrane filter (product name: Fuji Microfilter FM500, manufactured by Fuji Photo Film Co., Ltd.)
A hexane solution of water-repellent silicone resin with a thickness of 140 μm, a porosity of 75%, and an average pore diameter of 5 pm was inseminated and dried to give water repellency. A single layer of barrier was formed by adhering to the material (having a 100% carbonaceous material).

Then, on top of the barrier layer, a reaction layer (dry layer thickness 20 m),
A light shielding layer N (dry layer thickness: 51 Lm) and an adhesive layer were sequentially applied and dried.

Furthermore, the surface coated with each of the above layers in a dry state is swollen with water, and a cloth (Cotton Broad No. 100) is pressure laminated and adhered as a developing layer on top of it to form an integrated multilayer for blood urea nitrogen measurement. Created analysis elements.

The compositions and preparation methods of the coating liquids used to form the indicator layer, reaction layer, light shielding layer, and adhesive layer are shown below. The coating solution for each layer was applied to the dry layer thickness described above.

Indicator layer coating liquid Bromocresol Clean 60 mg Polyvinyl acetate/acrylic acid ester copolymer latex Solids content 50%, pH 4.4) 5 g 3.3-Dimethylglutaric acid 20 mg Water
2m vertical fork ILL Yuki gelatin 10g water
100m p-nonylphenoxy polyglycidol 0.3g urease
0.8g Ethylenediaminetetraha/tetrasodium salt 0.4g A coating solution having the above composition was adjusted to p) (8) using disodium orthophilic acid and sodium hydroxide.

1 Watarujage IL Yugata Titanium Dioxide Fine Powder 4g Gelatin
4gP-nonylphenoxypolyglycitol 0.15g water 40m
Gelatin 2.5g water
50m or p-nonylphenoxy polyglycidol 0.15g [Example 1] A multilayer analytical element for urea nitrogen analysis prepared by the above method was housed in a plastic frame having a circular opening in the center of the upper and lower surfaces. Then, a urea nitrogen analysis slide having the form shown at 20 in FIG. 1 was prepared.

An analysis slide carrier (made of aluminum, surface anodized) of the shape shown by 10 in Figs. 1 and 2,
A polyethylene film (thickness: 50 mm
JLm) was attached using double-sided adhesive tape as shown by 11 (hatched area) in FIG.

Using the analysis slide carrier to which this polyethylene film was adhered and the urea nitrogen analysis slide, automatic analysis of urea nitrogen was repeatedly performed on the reprinted control serum, Monitorol ■. This automatic analysis operation was carried out using a Fuji Drychem System automatic analyzer (Fuji Photo Film Co., Ltd.) equipped with an incubator described in the above-mentioned Japanese Patent Application Laid-Open No. 58-21566. Incubation was performed at 37°C for 6 minutes.

[Example 2] An analysis slide carrier was manufactured in the same manner as in Example 1 except that a polyethylene terephthalate film was used instead of the polyethylene film, and urea nitrogen measurements were performed using the same urea nitrogen analysis slide.

[Comparative Example 1] Urea nitrogen was measured in the same manner as in Example 1 using the same carrier as in Example 1 with the alumite processed surface exposed without attaching a polyethylene film. The measurement results of Comparative Example 2 and Comparative Example 1 are shown in Table 1 below.

Table 1 Urea nitrogen measurement values (sample: Monitorol-E-X) 1st 2nd 3rd Example 1 14.9 15.0 15.0 Example 2
14.9 14.9 15.0 Comparative Example 1 14.1
15.0 15.0 As is clear from Table 1, when the carriers of Examples 1 and 2 were used, substantially the same values were repeatedly obtained, indicating that the reproducibility of the measured values was high. . On the other hand, when the carrier of Comparative Example 1 is used, the first measured value is clearly lower than the second and subsequent measured values. Therefore, it is clear that the first measurement value of Comparative Example 1 was affected by the adsorption of ammonia gas onto the carrier surface.

[Example 3] The integrated multilayer analytical element for urea nitrogen analysis used in Examples 1 and 2 was made in the same manner as in Example 1, except that the following changes (1 and 2) were made. Created analysis slides.

1) The coloring reagent contained in the coloring indicator layer was changed from bromcresol green to bromphenol blue.

2) Urease was removed from the reaction layer, and the pH of the coating solution was changed to 10.0 to increase sensitivity.

Using the thus obtained ammonia analysis slide and the analysis slide carrier used in Example 1, Example 1
After conducting urea nitrogen analysis in the same manner as above, the ammonia gas adsorbed and remaining in the carrier was measured.

[Example 4] Ammonia gas adsorbed and remaining in the carrier was measured in the same manner as in Example 3, except that the same analysis slide carrier used in Example 2 was used.

[Comparative Example 2] The inside of the carrier was prepared in the same manner as in Example 3, except that a carrier was used in which the alumite (aluminum oxide) surface of the same multilayer analysis element as in Comparative Example 1 was exposed in the portion corresponding to the sample spotting surface. The residual ammonia gas adsorbed on the was measured.

The measurement results of Example 2 and Comparative Example 2 are shown in Table 2.

[Comparative Example 3] A polyvinyl chloride film (thickness: 190 JLm) was used instead of the polyethylene film, and the sample was adsorbed and remained in the carrier in the same manner as in Example 3, except that the same analysis slide carrier as used in Example 3 was used. Ammonia gas was measured.

Table 2 with margins below Surface material Residual ammonia amount Example 3 Polyethylene 0.0013 kg Example 4 PET O.0009 kg Comparative example 2
Aluminum oxide 0.3gg Comparative Example 3 Polyvinyl chloride 0.0056pg As is clear from Table 2,
This shows that there is almost no residual ammonia gas in the carriers of Examples 3 and 4. On the other hand, in the carrier of Comparative Example 2, it was confirmed that a large amount of ammonia gas remained due to adsorption to the carrier surface, and in the carrier of Comparative Example 3, it was confirmed that a considerable amount of ammonia gas remained due to adsorption to the carrier surface. Ta.

[Brief explanation of the drawing]

FIGS. 1 and 2 are perspective views schematically showing the structure of an analysis slide carrier, which is an example of the sealing device of the present invention. FIG. 3 is a perspective view schematically showing the structure of an analysis slide carrier which is another example of the sealing device of the present invention. 10.30: Carrier 20: Analysis slide 11.31: Non-adsorbent polymer layer 21: Spotting opening 12.32: Analysis slide confirmation opening 13.33: Lid

Claims (1)

[Claims] 1. A liquid sample is applied to a multilayer analytical element in the form of an analytical slide housed in a frame having openings on both upper and lower sides, and the reaction generated within the multilayer analytical element is evaluated by incubating the liquid sample. The analysis slide has the function of reducing evaporation of the liquid sample from the multilayer analysis element in the above-mentioned incubation step of the liquid sample analysis method comprising detecting the liquid sample so as to substantially cover the liquid sample application surface of the multilayer analysis element. An analysis slide, characterized in that the sealing device is disposed adjacent to the multilayer analytical element, and at least a portion of the surface of the sealing device facing the liquid sample applying surface of the multilayer analytical element is formed of a non-adsorbent polymer. closures. 2. The closure device according to claim 1, wherein the closure device moves together with the analysis slide during the incubation step. 3. The closure device according to claim 2, wherein the closure device is in the form of a housing that is opened at a portion facing the lower surface of the multilayer analysis element. 4. The sealing device according to claim 2, wherein the sealing device has a sheet shape and is capable of sealing the upper opening of the frame. 5. The seal according to claim 3, wherein the sealing device is made of a metal material, and a non-adsorptive polymer is arranged in a layer on the surface of the metal material. Stop. 6. The closure device according to claim 3, wherein the closure device is made of a non-adsorptive polymer. 7. The closure device according to claim 1, wherein the closure device moves relative to the analysis slide during the incubation step. 8. The sealing device according to any one of claims 1 to 7, wherein the non-adsorptive polymer is a polyolefin polymer. 9. The sealing device according to claim 8, wherein the non-adsorptive polyolefin polymer is polyethylene or polypropylene. 10. Claims 1 to 7, characterized in that the non-adsorptive polymer is a polyester polymer.
A sealing device described in any of the following paragraphs. 11. The above polyester polymers include polyethylene terephthalate, butylene terephthalate, polyethylene terephthalate,
The sealing device according to claim 10, characterized in that it is made of polybutylene terephthalate. 12. The closure device according to any one of claims 1 to 11, wherein the multilayer analytical element is for measuring ammonia or an ammonia-producing substrate. 13. Claims 1 to 1, characterized in that substantially the entire surface of the sealing device corresponding to the liquid sample applying surface of the multilayer analysis element is formed of a non-adsorbent polymer.
The sealing device described in any of Item 1.