JPS6342988A - Improved enzymatic pulping of bark fiber - Google Patents
Improved enzymatic pulping of bark fiberInfo
- Publication number
- JPS6342988A JPS6342988A JP18709886A JP18709886A JPS6342988A JP S6342988 A JPS6342988 A JP S6342988A JP 18709886 A JP18709886 A JP 18709886A JP 18709886 A JP18709886 A JP 18709886A JP S6342988 A JPS6342988 A JP S6342988A
- Authority
- JP
- Japan
- Prior art keywords
- xylan
- pectin
- degrading enzyme
- fibers
- pulping
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000835 fiber Substances 0.000 title claims description 44
- 238000004537 pulping Methods 0.000 title claims description 23
- 230000002255 enzymatic effect Effects 0.000 title claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 67
- 102000004190 Enzymes Human genes 0.000 claims description 67
- 238000000034 method Methods 0.000 claims description 35
- 229920001221 xylan Polymers 0.000 claims description 15
- 150000004823 xylans Chemical class 0.000 claims description 15
- 239000000428 dust Substances 0.000 claims description 13
- 230000000593 degrading effect Effects 0.000 claims description 7
- 229920001277 pectin Polymers 0.000 claims description 7
- 241000588698 Erwinia Species 0.000 claims description 6
- 239000001814 pectin Substances 0.000 claims description 6
- 235000010987 pectin Nutrition 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 230000000694 effects Effects 0.000 description 10
- 239000000126 substance Substances 0.000 description 10
- 239000000243 solution Substances 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000012670 alkaline solution Substances 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 2
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 2
- 241001265525 Edgeworthia chrysantha Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 108090000856 Lyases Proteins 0.000 description 2
- 102000004317 Lyases Human genes 0.000 description 2
- 240000000249 Morus alba Species 0.000 description 2
- 235000008708 Morus alba Nutrition 0.000 description 2
- 229920002230 Pectic acid Polymers 0.000 description 2
- 108010029182 Pectin lyase Proteins 0.000 description 2
- 241000588701 Pectobacterium carotovorum Species 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000001099 ammonium carbonate Substances 0.000 description 2
- 235000010216 calcium carbonate Nutrition 0.000 description 2
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 2
- 239000000920 calcium hydroxide Substances 0.000 description 2
- 235000011116 calcium hydroxide Nutrition 0.000 description 2
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 2
- 239000010318 polygalacturonic acid Substances 0.000 description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- 235000017550 sodium carbonate Nutrition 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 240000008564 Boehmeria nivea Species 0.000 description 1
- 240000006248 Broussonetia kazinoki Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- 240000000491 Corchorus aestuans Species 0.000 description 1
- 235000011777 Corchorus aestuans Nutrition 0.000 description 1
- 235000010862 Corchorus capsularis Nutrition 0.000 description 1
- 244000018436 Coriandrum sativum Species 0.000 description 1
- 235000002787 Coriandrum sativum Nutrition 0.000 description 1
- PQUCIEFHOVEZAU-UHFFFAOYSA-N Diammonium sulfite Chemical compound [NH4+].[NH4+].[O-]S([O-])=O PQUCIEFHOVEZAU-UHFFFAOYSA-N 0.000 description 1
- 240000009253 Morus australis Species 0.000 description 1
- 240000009257 Phormium tenax Species 0.000 description 1
- 235000000422 Phormium tenax Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 150000001340 alkali metals Chemical class 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 150000001342 alkaline earth metals Chemical class 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 235000012501 ammonium carbonate Nutrition 0.000 description 1
- 150000007514 bases Chemical class 0.000 description 1
- NKWPZUCBCARRDP-UHFFFAOYSA-L calcium bicarbonate Chemical compound [Ca+2].OC([O-])=O.OC([O-])=O NKWPZUCBCARRDP-UHFFFAOYSA-L 0.000 description 1
- 229910000020 calcium bicarbonate Inorganic materials 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- 235000012255 calcium oxide Nutrition 0.000 description 1
- GBAOBIBJACZTNA-UHFFFAOYSA-L calcium sulfite Chemical compound [Ca+2].[O-]S([O-])=O GBAOBIBJACZTNA-UHFFFAOYSA-L 0.000 description 1
- 235000010261 calcium sulphite Nutrition 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 235000005607 chanvre indien Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 108010038658 exo-1,4-beta-D-xylosidase Proteins 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 150000004679 hydroxides Chemical class 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- GSWAOPJLTADLTN-UHFFFAOYSA-N oxidanimine Chemical compound [O-][NH3+] GSWAOPJLTADLTN-UHFFFAOYSA-N 0.000 description 1
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical group O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000011736 potassium bicarbonate Substances 0.000 description 1
- 235000015497 potassium bicarbonate Nutrition 0.000 description 1
- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 235000011181 potassium carbonates Nutrition 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
- 229940086066 potassium hydrogencarbonate Drugs 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- BHZRJJOHZFYXTO-UHFFFAOYSA-L potassium sulfite Chemical compound [K+].[K+].[O-]S([O-])=O BHZRJJOHZFYXTO-UHFFFAOYSA-L 0.000 description 1
- 235000019252 potassium sulphite Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000009991 scouring Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Paper (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Abstract] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
産業上の利用分野
本発明はじん皮繊維の酵素パルプ化法の改良法に関する
ものである。さらに詳しくいえば、本発明は、じん皮繊
維の酵素パルプ化において、あらかじめキシラン分解酵
素を用いて該じん皮繊維に含まれるキシランを分解除去
することによって、次いで行われるペクチン質分解酵素
による該じん皮繊維のパルプ化を促進させる方法に関す
るものである。DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to an improved method for enzymatic pulping of dust fibers. More specifically, in the enzymatic pulping of dust fibers, the present invention involves decomposing and removing xylan contained in the dust fibers using a xylan-degrading enzyme in advance, and then decomposing the dust with a pectin-degrading enzyme. The present invention relates to a method for promoting pulping of skin fibers.
従来の技術
ペクチン質を多量に含有するミツマタやコウゾなどのし
ん皮繊維は、従来より和紙の原料として製紙産業におい
て広く用いられてきた。BACKGROUND OF THE INVENTION BACKGROUND OF THE INVENTION BACKGROUND OF THE INVENTION Traditionally, paper fibers such as Japanese mulberry and paper mulberry, which contain a large amount of pectin, have been widely used in the paper industry as raw materials for Japanese paper.
とのしん皮繊維には、ペクチン質がセルロースｔａｉ間
結合物質として存在しており、そのパルプ化は該ペクチ
ン質を除去することによって行われる。 。Pectin is present in the bark fibers as a binding substance between cellulose fibers, and pulping is performed by removing the pectin. .
じん皮繊維からパルプを製造する方法としては、化学的
処理による方法と、生化学的にしん皮繊維をパルプ化す
る、いわゆる発酵精練処理や酵素を用いて処理する方法
が知らｎている。前者の化学的処理方法としては、一般
に水酸１ヒナトリウム、炭酸す） ＩＪウム、生石灰又
は消石灰などの１ヒ学薬品を用いて、じん皮繊維を熟成
させてパルプ化する方法が用いられている。この熟成時
間は１時間〜−昼夜、通常数時間以内である。しかしな
がら、化学的処理方法は、このように処理時間が短いも
のの、排出液の汚濁が著しく、かつ収率が低いという欠
点がある。As methods for producing pulp from bark fibers, there are known methods such as chemical treatment, biochemical pulping of bark fibers, so-called fermentation scouring treatment, and treatment using enzymes. The former chemical treatment method generally involves ripening dust fibers and turning them into pulp using chemical chemicals such as monoarsenium hydroxide, sodium carbonate, quicklime, or slaked lime. There is. The aging time is from 1 hour to day and night, usually within several hours. However, although the chemical treatment method has such a short treatment time, it has the drawbacks of significant pollution of the effluent and low yield.
一方、後者の生ｆヒ学的にしん皮繊維をパルプ化する方
法は、排出液の汚濁が少なく、その上収率が高いものの
、−殻内に、化学的処理方法に比べて処理時間が日、週
、場合により月単位であるなど極めて長いという欠点ｔ
ｉしている。On the other hand, the latter method of biochemically pulping the bark fibers has less contamination of the effluent and a higher yield, but it requires less processing time than the chemical treatment method. The disadvantage is that it is extremely long, such as days, weeks, or even months.
i am doing
じん皮繊維の酵素パルプ化法は、ペクチン酸すアーゼヤ
ベクチンリアーゼなどのペクチン質分解酵素によって行
われるが、前記したような欠点を育しており、したがっ
てじん皮繊維のパルプ化における反応速度を高める次め
に、例えば該じん皮繊維中に酵素などが浸入しやすいよ
うに、じん皮繊維の物理的又は化学的状態を改良する方
法や、酵素活性が最高に発現しうる最適ｐＨによる処理
方法などが提案されている。Enzymatic pulping of dust fibers, which is carried out using pectin-degrading enzymes such as pectin acidase and pectin lyase, suffers from the drawbacks mentioned above and therefore reduces the reaction rate in the pulping of dust fibers. Next, for example, a method of improving the physical or chemical state of the dust fibers so that enzymes etc. can easily penetrate into the dust fibers, and a treatment method using an optimum pH that allows the enzyme activity to be expressed at its maximum. etc. have been proposed.
しかしながら、これらの方法においては、パルプ化の反
応速度はかなり向上するものの、結束繊維率、すなわち
繊維結合物質であるペクチン質の残存率を低減させるの
に限界があり、この残存率をいかにしてさらに低減させ
るかが重要な課題となっていた。However, although these methods considerably improve the reaction rate of pulping, there is a limit to reducing the binding fiber rate, that is, the residual rate of pectin, which is a fiber binding substance, and it is difficult to determine how to reduce this residual rate. An important issue was how to reduce it further.
発明が解決しようとする問題点
本発明の目的は、このような事情のもとで、じん皮繊維
の酵素パルス化において、ペクチン質分解酵素の働きを
高めることによシ、結束繊維率を従来の酵素パルプ化法
に比べて、さらに低減させる方法を提供することにある
。Problems to be Solved by the Invention Under these circumstances, the purpose of the present invention is to increase the activity of pectin-degrading enzymes in enzymatic pulsing of dust fibers, thereby reducing the binding fiber ratio to the conventional level. The object of the present invention is to provide a method for further reducing the amount of waste compared to the enzymatic pulping method.
問題点を解決するための手段
本発明者らは、ペクチン質分解酵素によるしん皮繊維の
パルプ化において、結束繊維率を低減させる方法につい
て鋭意研究を重ねた結果、該ペクチン質にはヘミセルロ
ースの１種であるキシランが、これまで提案されてきた
形態とは異なった形で結合していることを明らかにした
。そして該成分がペクチン質分解酵素の作用に対して悪
影響全与えることに着目し、これをあらかじめキシラン
分解酵素による処理で分解除去することにより、前記目
的全達成しうろことを見出し、この知見に基づいて本発
明を完成するに至った。Means for Solving the Problems The present inventors have conducted intensive research on a method for reducing the binding fiber ratio in pulping pulp fibers using a pectin-degrading enzyme. The researchers revealed that the xylan species binds in a different way than previously proposed. They focused on the fact that this component had a negative effect on the action of pectin-degrading enzymes, and found that by decomposing and removing it in advance by treatment with xylan-degrading enzymes, all of the above objectives could be achieved. As a result, the present invention was completed.
すなわち、本発明は、ペクチン質分解酵素を用いてじん
皮繊維をパルプ化するに当り、あらかじめキシラン分解
酵素により該繊維に含まれるキシラン成分を分解除去す
ることを特徴とする改良法を提供するものである。That is, the present invention provides an improved method characterized in that, when pulping bark fibers using a pectin-degrading enzyme, xylan components contained in the fibers are decomposed and removed in advance by a xylan-degrading enzyme. It is.
以下１本発明の詳細な説明する。Hereinafter, one aspect of the present invention will be explained in detail.
本発明方法において、原料として用いるしん皮繊維は、
ペクトセルロース系繊維であって、例えばガンピ、ミッ
マタ、コウゾ、桑などのじん皮、ペンダ、ラミー、ジュ
ート、ニュージランド麻などのいわゆる軟質系麻類のよ
うな繊維間のこう着物質が主としてペクチン質である植
物繊維である。In the method of the present invention, the shinpi fiber used as a raw material is
It is a pectocellulose fiber, and the adhesion substance between the fibers is mainly pectic, such as bark of gampi, mimmata, mulberry, mulberry, so-called soft hemp such as penda, ramie, jute, and New Zealand hemp. It is a certain plant fiber.
本発明方法において用いるペクチン質分解酵素としては
、アルカリ領域に酵素活性の至適ｐＨｅもつペクチン酸
リアーゼやペクチンリアーゼが挙げられる。これらの酵
素はエルウィニア（Ｅｒｗｉ −ｎｉａ ）属の細菌、
例えばエルウィニア・カロトボラ（ｃａｒｏｔｏｖｏｒ
ａ ）、クリサンテマイ（Ｏｈｒｙｓａ−ｎｔｈｅｍｉ
）、アミロポラ（ａｍｙｌｏｖｏｒａ ）、ヘルビコ
ラ（ｈｅｒｂｉｃｏｌａ ）、デイゾルベンス（ｄｉｓ
ｓｏ−１ｖｅｎｓ ）などによって産生される。Examples of the pectin-degrading enzyme used in the method of the present invention include pectic acid lyase and pectin lyase, which have an optimal pH for enzymatic activity in the alkaline region. These enzymes are produced by bacteria of the genus Erwi-nia,
For example, Erwinia carotovora
a), Ohrysa-nthemi
), amylovora, herbicola, dis
so-1vens).
一方、キシラン分解酵素については種々の植物組織から
得られるものや、黒カビのアスペルギルス・ニガー（Ａ
ｓｐｅｒｇｉｌｌｕｓ ｎｉｇｅｒ ）などによって産
生されるものが知られており、さらに、前記のエルウィ
ニア属の細菌はペクチン質分解酵素のほかに、キシラン
分解酵素を同時に産生ずることも知られている。On the other hand, xylan-degrading enzymes are obtained from various plant tissues and the black mold Aspergillus niger (A
Spergillus niger), and the bacteria of the genus Erwinia are also known to simultaneously produce xylan-degrading enzymes in addition to pectin-degrading enzymes.
本発明方法を実施するには、まず原料のしん皮繊維をあ
らかじめ水又は希アルカリ水溶液中に浸せきして膨潤状
態にしておくことが好ましい。この希アルカリ水溶液に
用いる塩基性化合物としては、アルカリ金属、アルカリ
土類金属又はアンモニウムの水酸化物やそれらの弱酸塩
があり、具体的には水酸化ナトリウム、水酸化カリウム
、水酸化カルシウム、水酸化アンモニウム、炭酸ナトリ
ラム、炭酸カリウム、炭酸カルシウム、炭酸アンモニウ
ム、炭酸水素ナトリウム、炭酸水素カリウム、炭酸水素
カルシウム、炭酸水素アンモニウム、亜硫酸ナトリウム
、亜硫酸カリウム、亜硫酸カルシウム、亜硫酸アンモニ
ウムなどが挙げられる。In order to carry out the method of the present invention, it is preferable to first soak the raw material shinpi fiber in water or a dilute aqueous alkaline solution in advance to bring it into a swollen state. The basic compounds used in this dilute alkaline aqueous solution include hydroxides of alkali metals, alkaline earth metals, or ammonium, and their weak acid salts, and specifically, sodium hydroxide, potassium hydroxide, calcium hydroxide, hydroxide Examples include ammonium oxide, sodium carbonate, potassium carbonate, calcium carbonate, ammonium carbonate, sodium hydrogen carbonate, potassium hydrogen carbonate, calcium hydrogen carbonate, ammonium hydrogen carbonate, sodium sulfite, potassium sulfite, calcium sulfite, ammonium sulfite, and the like.
また、希アルカリ水溶液の濃度は数重量係程度が好まし
い。もちろんそれより高い濃度でも効果はあるが、経済
的に不利である。さらに、水又は希アルカリ水溶液の量
については、じん皮繊維が万遍なく浸せきされる程度以
上であれば特に制限はなく、また浸せき温度は通常室温
でよいが、必要に応じて加熱してもよく、浸せき時間は
室温の場合、１時間以上数日間が望ましい。Further, the concentration of the dilute alkali aqueous solution is preferably about several weight coefficients. Of course, higher concentrations are also effective, but are economically disadvantageous. Furthermore, there is no particular restriction on the amount of water or dilute aqueous alkaline solution as long as the dust fibers are evenly immersed, and the immersion temperature is usually room temperature, but it may be heated if necessary. Generally, the soaking time is desirably 1 hour or more and several days at room temperature.
次に、このように水又は希アルカリ水溶液中に浸せきし
、膨潤状態にされたしん皮繊維に、まずキシラン分解酵
素を作用させてキシラン成分を分解除去したのち、ペク
チン質分解酵素全作用させて該しん皮鷹維をパルプ化す
るが、この際２種の方法を用いることができる。すなわ
ち、第１の方法はキシラン分解酵素及びペクチン質分解
酵素をそれぞれ単独で用いる方法であり、第２の方法は
エルウィニア属細菌から得られるキシラン分解酵素及び
ペクチン質分解酵素を含む粗酵素液を用いる方法である
。Next, the bark fibers, which have been immersed in water or a dilute aqueous alkaline solution to become swollen, are first treated with a xylan-degrading enzyme to decompose and remove the xylan component, and then a pectin-degrading enzyme is fully acted on. The shinpi taka fiber is pulped, and two methods can be used at this time. That is, the first method uses a xylan-degrading enzyme and a pectin-degrading enzyme alone, and the second method uses a crude enzyme solution containing a xylan-degrading enzyme and a pectin-degrading enzyme obtained from Erwinia bacteria. It's a method.
第１の方法については、前記のようにして水又は希アル
カリ水溶液中に浸せきして膨潤状態にされたしん皮繊維
に、まずキシラン分解酵素を加えて処理し、キシラン成
分を選択的かつ効率的に分解除去する。この際、キシラ
ン分解酵素は中性ｐＨ領域で酵素活性を有するので、該
処理は中性ｐＨ近辺で行うことが望ましい。処理温度は
通常？５〜３０℃の範囲で選ばれ、また処理時間は３〜
６時間程度で十分である。次にこの処理液にペクチン質
分解酵素を添加してパルプ化を行う。該ペクチン質分解
酵素は酵素活性がアルカリｐＨ領域にあるので、ｐＨ’
ｉ９．０〜９．５ の範囲に調整して処理を行うこと
が重要である。他の処理条件はキシラン分解酵素の場合
と同様である。In the first method, the shinpi fibers that have been soaked in water or a dilute alkaline aqueous solution to become swollen as described above are treated by adding a xylan-degrading enzyme to selectively and efficiently remove xylan components. Decompose and remove. At this time, since the xylan degrading enzyme has enzymatic activity in the neutral pH region, it is desirable to carry out the treatment at around neutral pH. What is the normal processing temperature? The temperature is selected from 5 to 30℃, and the processing time is from 3 to 30℃.
About 6 hours is sufficient. Next, a pectin-degrading enzyme is added to this treatment liquid to perform pulping. The enzyme activity of the pectin-degrading enzyme is in the alkaline pH region, so the pH'
It is important to perform processing by adjusting the i to a range of 9.0 to 9.5. Other treatment conditions are the same as in the case of xylan degrading enzyme.
第２の方法は該膨潤状態のしん皮繊維に、エルウィニア
属細菌から得られる粗酵素液を加えて処理を行う方法で
ある。この粗酵素液にはキシラン分解酵素及びペクチン
質分解酵素が含まれており、前者のキシラン分解酵素は
中ｉｐＨ領域で、後者のペクチン質分解酵素はアルカリ
ｐＨ領域で酵素活性を発揮するので、まず中性で処理を
行って、キシラン分解酵素によるキシラン成分の分解除
去を行う。この際の処理温度及び処理時間は、第１の方
法におけるキシラン分解酵素による処理の場合と同様で
ある。次に、ｐＨ’ｉ９．０〜９，５の範囲に調整する
のみで（新たに酵素は加えない）ペクチン質分解酵素に
よるパルプ化処理過程に移行する。この際の処理温度及
び処理時間も第１の方法ておけるペクチン質分解酵素に
よる処理の場合と同様である。The second method is to treat the swollen coriander fibers by adding a crude enzyme solution obtained from bacteria belonging to the genus Erwinia. This crude enzyme solution contains xylan-degrading enzyme and pectin-degrading enzyme, and the former xylan-degrading enzyme exhibits enzymatic activity in the medium IP range, and the latter pectin-degrading enzyme exhibits enzymatic activity in the alkaline pH range. Treatment is carried out in neutral conditions, and xylan components are decomposed and removed by xylan-degrading enzymes. The treatment temperature and treatment time at this time are the same as in the case of treatment with xylan degrading enzyme in the first method. Next, by simply adjusting the pH'i to a range of 9.0 to 9.5 (no new enzymes are added), the pulping process using a pectin-degrading enzyme is started. The treatment temperature and treatment time at this time are also the same as in the case of treatment with a pectin-degrading enzyme in the first method.
発明の効果
本発明方法によると、まずキシラン分解酵素によって、
ペクチン質分解酵素によるしん皮繊維のパルプ化に悪影
響を与える該繊維中のキシラン成分を分解除去し、次い
でペクチン質分解酵素を作用させてパルプ化を行うので
、従来の酵素パルプ化法に比べて、反応効率が高く、結
束繊維率を著しく低減することができ、得られ次パルプ
からは地合いの良好な物理特性に優れた和紙が形成され
る。Effects of the Invention According to the method of the present invention, first, xylan degrading enzyme
The xylan component in the fibers that has an adverse effect on pulping by pectin-degrading enzymes is decomposed and removed, and then the pectin-degrading enzymes are applied to pulp the fibers, which is more effective than the conventional enzymatic pulping method. The reaction efficiency is high, the binding fiber ratio can be significantly reduced, and the resulting pulp forms Japanese paper with good texture and excellent physical properties.
実施例 次に実施例により本発明をさらに詳細に説明する。Example Next, the present invention will be explained in more detail with reference to Examples.
実施例１
０．１重量係水酸化ナトリウム水溶液中に一夜浸せきし
たミツマタじん皮（総乾量として２０２）を水洗したの
ち、酵素量として３５単位の黒カビ起源のキシラン分解
酵素（β−キシロシダーゼ）と共に回転振とう条件下で
、ｐＨ６，０、温度３０℃において３時間処理した。次
いでエルウィニア・カロトボラＦＥＲＭ Ｐ−７５７６
株起源のペクチン質分解酵素（ペクチン酸リアーゼ活性
として８００単位）を添加し、ｐＨ１９，５に調整した
のち、回転振とり条件下で、３０℃において３時間処理
を行った。Example 1 Mitsumata bark (total dry weight: 202) soaked overnight in a 0.1 weight aqueous sodium hydroxide solution was washed with water, and then mixed with xylan-degrading enzyme (β-xylosidase) originating from black mold, with an enzyme amount of 35 units. It was treated under rotary shaking conditions at pH 6.0 and temperature 30° C. for 3 hours. Then Erwinia carotovora FERM P-7576
A strain-derived pectin-degrading enzyme (800 units of pectic acid lyase activity) was added and the pH was adjusted to 19.5, followed by treatment at 30° C. for 3 hours under rotating shaking conditions.
パルプ化終了後、反応系から繊維全ろ取し、結束愼維率
を測定し、ろ液については反応中繊維より遊離した成分
の全Ｍ機炭素量全定量し、これらをパルプ化反応進行度
の示標とした。ここでは結束繊維率が低いほど、また遊
離した繊維成分量が多いほど、パルプ化の進行度が高い
ことになる。After the completion of pulping, all the fibers are filtered from the reaction system, the binding fiber ratio is measured, and the total amount of carbon in the components liberated from the fibers during the reaction is quantified for the filtrate. It was used as an indicator. Here, the lower the binding fiber ratio and the greater the amount of free fiber components, the higher the progress of pulping.
また、対照としてペクチン質分解酵素のみによる６時間
の１段階処理（従来の酵素法に相当）を行った。両法の
効果の比較結果を別表に示す。In addition, as a control, a 6-hour one-step treatment (corresponding to a conventional enzyme method) using only a pectin-degrading enzyme was performed. The results of comparing the effects of both methods are shown in the attached table.
実施例２
実施例１における黒カビ起源のキシラン分解酵素の代り
に、エルウィニア属細菌起源の粗酵素液に含まれている
キシラン分解酵素（活性として３５単位）ｔ−粗酵素液
の状態で用いた。なお、該粗酵素液にはキシラン分解酵
素とともに８００単位のペクチン質分解酵素が含まれて
いる。Example 2 In place of the xylan-degrading enzyme originating from black mold in Example 1, xylan-degrading enzyme (35 units in terms of activity) contained in the crude enzyme solution originating from bacteria of the genus Erwinia was used in the form of a t-crude enzyme solution. The crude enzyme solution contained 800 units of pectin-degrading enzyme as well as xylan-degrading enzyme.
まず、キシラン分解酵素が作用しうるｐＨ６，５の０．
１Ｍリン酸カリウム緩衝液中で、実施例１と同様にして
、３時間キシランの分解処理全行つ念のち、アンモニア
水にて反応系のｐＨｙ＆：、９．５に調整し、今度は該
粗酵素液中のペクチン質分解酵素が活性を示すことので
きる条件で、さらに３時間処理を行った。この方法によ
るパルプ化の進行度を別表に示す。なお、対照は実施例
１と同様であるＯ
実施例３
実施例２とほぼ同じ手法の２段階法を、総覧量１４に２
のミツマタじん皮を用いたペンチ処理に適用した。なお
、各段階における処理はそれぞれ１２時間行った。First, at a pH of 6.5, at which xylan degrading enzymes can act.
After carrying out the complete decomposition treatment of xylan in 1M potassium phosphate buffer for 3 hours in the same manner as in Example 1, the pH of the reaction system was adjusted to 9.5 with aqueous ammonia, and this time the crude The treatment was continued for an additional 3 hours under conditions that allowed the pectin-degrading enzyme in the enzyme solution to exhibit activity. The progress of pulping by this method is shown in the attached table. The control was the same as in Example 1. Example 3 The two-step method, which is almost the same as in Example 2, was applied to a total volume of 14.
It was applied to pliers treatment using Mitsumata bark. In addition, the treatment at each stage was performed for 12 hours.
また、対照としてペクチン質分解酵素のみによる２４時
間の１段階処理を行った。これらの結果を別表に示す。In addition, as a control, a 24-hour one-step treatment using only a pectin-degrading enzyme was performed. These results are shown in the attached table.
Claims (1)
するに当り、あらかじめキシラン分解酵素により該繊維
に含まれるキシラン成分を分解除去することを特徴とす
るじん皮繊維の酵素パルプ化法。 ２ キシラン分解酵素及びペクチン質分解酵素として、
エルウイニア（Ｅｒｗｉｎｉａ）属細菌から得られる前
記両酵素を含む粗酵素液を使用する特許請求の範囲第１
項記載の酵素パルプ化法。[Claims] 1. An enzyme for dust fibers, which is characterized in that, when pulping dust fibers using a pectin-degrading enzyme, xylan components contained in the fibers are decomposed and removed by a xylan-degrading enzyme in advance. Pulping method. 2 As xylan degrading enzyme and pectin degrading enzyme,
Claim 1, which uses a crude enzyme solution containing both of the enzymes obtained from bacteria of the genus Erwinia.
The enzymatic pulping method described in Section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18709886A JPS6342988A (en) | 1986-08-08 | 1986-08-08 | Improved enzymatic pulping of bark fiber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18709886A JPS6342988A (en) | 1986-08-08 | 1986-08-08 | Improved enzymatic pulping of bark fiber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6342988A true JPS6342988A (en) | 1988-02-24 |
Family
ID=16200069
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP18709886A Pending JPS6342988A (en) | 1986-08-08 | 1986-08-08 | Improved enzymatic pulping of bark fiber |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6342988A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5103883A (en) * | 1989-12-27 | 1992-04-14 | Kone Oy | Method for the debarking of logs |
| EP2261359A1 (en) | 1998-06-10 | 2010-12-15 | Novozymes A/S | Mannanases |
| WO2014100018A1 (en) | 2012-12-19 | 2014-06-26 | Danisco Us Inc. | Novel mannanase, compositions and methods of use thereof |
| WO2017079751A1 (en) | 2015-11-05 | 2017-05-11 | Danisco Us Inc | Paenibacillus sp. mannanases |
| WO2017079756A1 (en) | 2015-11-05 | 2017-05-11 | Danisco Us Inc | Paenibacillus and bacillus spp. mannanases |
| WO2017106676A1 (en) | 2015-12-18 | 2017-06-22 | Danisco Us Inc | Polypeptides with endoglucanase activity and uses thereof |
| CN109183485A (en) * | 2018-08-06 | 2019-01-11 | 华南理工大学 | The fully chlorine-free bleaching method and application that a kind of china picture-character paper is starched with bark |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5328701A (en) * | 1976-08-30 | 1978-03-17 | Kogyo Gijutsuin | Process for making paper pulp from libriform fiber |
| JPS54147202A (en) * | 1978-05-11 | 1979-11-17 | Kogyo Gijutsuin | Production of defibrilated fiber |
| JPS58144192A (en) * | 1982-02-22 | 1983-08-27 | 工業技術院長 | Pulping of bast fiber |
-
1986
- 1986-08-08 JP JP18709886A patent/JPS6342988A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5328701A (en) * | 1976-08-30 | 1978-03-17 | Kogyo Gijutsuin | Process for making paper pulp from libriform fiber |
| JPS54147202A (en) * | 1978-05-11 | 1979-11-17 | Kogyo Gijutsuin | Production of defibrilated fiber |
| JPS58144192A (en) * | 1982-02-22 | 1983-08-27 | 工業技術院長 | Pulping of bast fiber |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5103883A (en) * | 1989-12-27 | 1992-04-14 | Kone Oy | Method for the debarking of logs |
| EP2261359A1 (en) | 1998-06-10 | 2010-12-15 | Novozymes A/S | Mannanases |
| EP2284272A1 (en) | 1998-06-10 | 2011-02-16 | Novozymes A/S | Mannanases |
| EP2287318A1 (en) | 1998-06-10 | 2011-02-23 | Novozymes A/S | Mannanases |
| WO2014100018A1 (en) | 2012-12-19 | 2014-06-26 | Danisco Us Inc. | Novel mannanase, compositions and methods of use thereof |
| WO2017079751A1 (en) | 2015-11-05 | 2017-05-11 | Danisco Us Inc | Paenibacillus sp. mannanases |
| WO2017079756A1 (en) | 2015-11-05 | 2017-05-11 | Danisco Us Inc | Paenibacillus and bacillus spp. mannanases |
| WO2017106676A1 (en) | 2015-12-18 | 2017-06-22 | Danisco Us Inc | Polypeptides with endoglucanase activity and uses thereof |
| WO2018112123A1 (en) | 2016-12-15 | 2018-06-21 | Danisco Us Inc. | Polypeptides with endoglucanase activity and uses thereof |
| CN109183485A (en) * | 2018-08-06 | 2019-01-11 | 华南理工大学 | The fully chlorine-free bleaching method and application that a kind of china picture-character paper is starched with bark |
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