JPS6339869B2 - - Google Patents
Info
- Publication number
- JPS6339869B2 JPS6339869B2 JP55086332A JP8633280A JPS6339869B2 JP S6339869 B2 JPS6339869 B2 JP S6339869B2 JP 55086332 A JP55086332 A JP 55086332A JP 8633280 A JP8633280 A JP 8633280A JP S6339869 B2 JPS6339869 B2 JP S6339869B2
- Authority
- JP
- Japan
- Prior art keywords
- specimen
- smearing
- capillary tube
- sample
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000002360 preparation method Methods 0.000 claims description 22
- 238000012546 transfer Methods 0.000 claims description 9
- 239000003595 mist Substances 0.000 claims description 8
- 210000004369 blood Anatomy 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 210000002700 urine Anatomy 0.000 claims description 2
- 230000032258 transport Effects 0.000 claims 1
- 238000000034 method Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 239000012491 analyte Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Details Or Accessories Of Spraying Plant Or Apparatus (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】
本発明は、プレパラート上に液体状の被検体を
塗沫する塗沫装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a smearing device for smearing a liquid specimen onto a preparation.
臨床検査などのように多量の計測を常時行なう
必要のある分野では処理能力の高い自動分析装置
の出現が望まれている。これに応えて、一部では
血液像自動分析装置なるものも出現し実用化され
ている。しかし、このような周知の装置も含め、
処理の一層の高速化を実現化するためには、標本
作成の高速化が大きな鍵となつている。 In fields such as clinical testing, where large quantities of measurements need to be taken at all times, the emergence of automatic analyzers with high throughput is desired. In response to this demand, automatic blood image analyzers have also been developed and put into practical use. However, including such well-known devices,
In order to realize even faster processing, speeding up specimen creation is a key factor.
従来の標本作成法は主としてスピン法とウエツ
ジ法であつた。スピン法は、プレパラートに被検
体を１滴落し、これを高速で回転させて被検体を
プレパラート上に薄く塗布させるものであり、他
方のウエツジ法は、被検体をプレパラート上に１
滴落し、その上に他のガラス板を滑らせながら載
置するなどしてプレパラート上に塗抹して標本を
作成するものである。 Conventional specimen preparation methods have mainly been the spin method and the wedge method. In the spin method, one drop of the specimen is placed on a preparation and the drop is rotated at high speed to coat the specimen thinly on the preparation.In the wedge method, one drop of the specimen is placed on the preparation.
A specimen is created by dropping a drop, placing another glass plate on top of it, and smearing it onto a preparation.
しかしながら、いずれの方法によつても１つの
プレパラート上には１つの被検体しか塗抹でき
ず、更に塗抹の自動化の場合には複雑で精密な機
構部を必要とし、また自動化したものであつても
被検体を一様に薄く塗抹することは容易ではなく
時間もかかるという問題があつた。 However, with either method, only one specimen can be smeared on one preparation, and in the case of automated smearing, a complex and precise mechanism is required. There was a problem in that it was not easy to smear the subject uniformly and thinly, and it took time.
本発明は、このような点に鑑みてなされたもの
で、簡単な構成で高速処理の可能なスプレー式の
塗沫装置を提供することを目的とする。 The present invention has been made in view of these points, and an object of the present invention is to provide a spray-type smearing device that has a simple configuration and is capable of high-speed processing.
本発明の他の目的は、均一標本すなわち被検体
中の細胞成分（例えば血球）などが均一に分散し
た塗沫標本の得られる塗沫装置を提供することに
ある。 Another object of the present invention is to provide a smear device capable of obtaining a uniform specimen, that is, a smear in which cell components (eg, blood cells) in a subject are uniformly dispersed.
本発明の更に他の目的は、１枚のプレパラート
上に複数個の塗沫が可能な塗沫装置を提供するこ
とにある。 Still another object of the present invention is to provide a smearing device capable of smearing a plurality of smears onto a single preparation.
以下図面を用いて本発明を詳細に説明する。第
１図は本発明に係る塗沫装置の一実施例を示す要
部構成図である。第１図において、１は移送ベル
ト、２は被検体の塗沫されるプレパラート、３は
マスク、４はサンプル管、７は空気ノズルであ
る。 The present invention will be explained in detail below using the drawings. FIG. 1 is a diagram showing the configuration of essential parts of an embodiment of a smearing device according to the present invention. In FIG. 1, 1 is a transfer belt, 2 is a preparation onto which a subject is smeared, 3 is a mask, 4 is a sample tube, and 7 is an air nozzle.
移送ベルト１は適宜の駆動手段（図示せず）に
よつて間欠駆動されるように構成されたもので、
このベルト上にプレパラート２を載置して所定距
離だけ移動するようになつている。サンプル管４
は血液、尿などの被検体を導くもので、サンプル
管内部に設けられた毛細管５は毛細管現象で被検
体を含んでいる。このサンプル管４としては、注
射針、細いパイプあるいはフエルト、フアイバー
などを結束して内部に埋設してなるパイプなどを
使用することができる。なお、サンプル管４は移
送ベルト１の上部にほぼ平行して着脱自在に取り
付けられていて、被検体交換に際してはサンプル
管ごと交換できるようになつている。 The transfer belt 1 is configured to be driven intermittently by a suitable driving means (not shown).
The preparation 2 is placed on this belt and moved by a predetermined distance. sample tube 4
is for introducing a sample such as blood or urine, and the capillary tube 5 provided inside the sample tube contains the sample through capillary action. As the sample tube 4, a syringe needle, a thin pipe, or a pipe formed by bundling felt, fiber, etc. and embedding it inside can be used. The sample tube 4 is detachably attached almost parallel to the upper part of the transfer belt 1, so that the entire sample tube can be replaced when replacing the sample.
空気ノズル７はパイプ状に形成され、サンプル
管の毛細管５とほぼ直角方向でその先端部が互い
に隣接するように配設され、ガスボンベあるいは
コンプレツサーなどから空気を送り込んでノズル
先端から圧縮空気をサンプル管４の先端部６に向
けて噴射できるように配設されている。また、空
気ノズル７は、被検体ミストの付着厚さを常に一
定にするために圧縮空気の強さ（圧力）及び噴射
時間が前記駆動手段により制御されるようになつ
ている。このときスプレーの原理で毛細管５内の
被検体が霧状になつてプレパラート上に付着す
る。マスク３は中央部に貫通孔を有する板で構成
され、プレパラートの塗沫部範囲を限定すると共
に周辺飛散を防止するためのもので、プレパラー
ト上に付着した被検体ミストには接触することが
ないような離間間隔をもつて着脱可能にプレパラ
ート上に取り付けられている。 The air nozzle 7 is formed into a pipe shape, and is disposed in a direction substantially perpendicular to the capillary tube 5 of the sample tube so that its tips are adjacent to each other. Air is sent from a gas cylinder or a compressor, and compressed air is supplied from the tip of the nozzle to the sample tube. It is arranged so that it can inject toward the tip part 6 of 4. Further, the air nozzle 7 is configured such that the strength (pressure) and the jetting time of the compressed air are controlled by the driving means in order to keep the adhesion thickness of the object mist constant at all times. At this time, the subject within the capillary tube 5 becomes atomized and adheres to the preparation due to the principle of spraying. The mask 3 is composed of a plate with a through hole in the center, and is used to limit the range of the smeared part of the preparation and to prevent scattering around the area, so that it does not come into contact with the subject mist adhering to the preparation. They are removably attached to the slides with a spacing of
このような構成において、空気ノズル７を駆動
して圧縮空気を噴射させプレパラート上面に被検
体ミストを一定量付着させる。その後空気の噴射
を止めて、移送ベルト１を駆動し所定量だけ移動
してプレパラートを次の塗沫位置まで移送する。
次いで、再び空気ノズル７を駆動し、圧縮空気を
噴射させ上述と同様の被検体の塗沫を行なう。 In such a configuration, the air nozzle 7 is driven to inject compressed air to deposit a certain amount of subject mist on the upper surface of the preparation. Thereafter, the air injection is stopped, and the transfer belt 1 is driven to move by a predetermined amount to transfer the preparation to the next smearing position.
Next, the air nozzle 7 is driven again to inject compressed air to smear the subject as described above.
このように空気ノズル７と移送ベルトを交互に
間欠的に駆動することにより塗沫標本２ａを連続
的に作成することができる。なお、他の被検体と
交換する場合は、噴霧時以前に適宜の手段（図示
せず）によつてサンプル管４を交換配置する。 By alternately and intermittently driving the air nozzle 7 and the transfer belt in this manner, the smear specimen 2a can be continuously created. In addition, when replacing the sample tube 4 with another sample, the sample tube 4 is replaced by appropriate means (not shown) before spraying.
第２図は本発明の他の実施例を示す構成図で、
サンプル管と空気ノズルを一体化した点を除いて
は第１図装置と同じである。第２図において、噴
霧ノズル２１は、中央部に毛細管２２を有し、こ
の毛細管２２を取り囲むように外管２３との間に
空気路２４を形成してなるもので、これによれば
空気路２４の先端部２５より圧縮空気を噴射する
ことにより毛細管２２に含まれた被検体を霧状に
してプレパラートに付着させることができる。 FIG. 2 is a configuration diagram showing another embodiment of the present invention,
This device is the same as the device shown in FIG. 1 except that the sample tube and air nozzle are integrated. In FIG. 2, the spray nozzle 21 has a capillary tube 22 in the center, and an air passage 24 is formed between the capillary tube 22 and an outer tube 23 so as to surround the capillary tube 22. By injecting compressed air from the tip 25 of the capillary tube 24, the subject contained in the capillary tube 22 can be atomized and adhered to the preparation.
なお、実施例では、サンプル管と空気ノズルあ
るいは噴霧ノズルを固定とし移送ベルトを駆動し
てプレパラートを移動するようにしたが、これに
限らず、逆にプレパラートを固定しサンプル管と
空気ノズルあるいは噴霧ノズルを移動するように
構成してもよい。 In the example, the sample tube and the air nozzle or spray nozzle are fixed and the transfer belt is driven to move the preparation. The nozzle may be configured to move.
また、これら２つの移動動作を適宜に組合せて
行ない得る構成としてもよい。 Further, a configuration may be adopted in which these two movement operations can be performed in an appropriate combination.
以上の説明から明らかなように、本発明のスプ
レー式の塗沫装置は次のような特徴及び効果があ
る。 As is clear from the above description, the spray-type smearing device of the present invention has the following features and effects.
(1) 構成が簡単で、塗沫動作が短時間であること
から、安価で高速の塗沫装置を実現することが
できる。(1) Since the configuration is simple and the smearing operation is short, an inexpensive and high-speed smearing device can be realized.
(2) スプレー式で被検体ミストを作り、これをプ
レパラート上に塗沫するので、塗沫量や分布が
均一な標本を容易に作成することができる。(2) Since the subject mist is created using a spray method and smeared onto the preparation, it is possible to easily prepare specimens with uniform smear amount and distribution.
(3) スプレー式の塗沫のため、被検体の細胞組織
を押し潰し破壊することなくプレパラート上に
塗沫することができる。(3) Since it is a spray-type smear, it can be smeared onto the preparation without crushing and destroying the cell tissue of the subject.
(4) マスクを使用して塗沫するため、１枚のプレ
パラートに複数個の塗沫標本を作ることができ
る。(4) Since the smear is applied using a mask, multiple smear specimens can be made on one slide.
(5) 毛細管を利用しているので、サンプル管端面
からの被検体のぼた落ちがない。(5) Since a capillary tube is used, there is no dripping of the sample from the end of the sample tube.
(6) サンプル管に被検体を採集する場合、毛細管
端面を被検体に接触させるだけで、後は毛細管
現象により自動的に被検体を吸い込むのため、
採集作業の簡易化及び高能率化が図れる。(6) When collecting a analyte into a sample tube, all you have to do is bring the end of the capillary tube into contact with the analyte, and the analyte is automatically sucked in by capillary action.
Collection work can be simplified and highly efficient.
(7) サンプル管と空気ノズルを複数組配設するこ
ともでき、これによれば同一プレパラート上に
それぞれ異なる被検体を同時に塗沫することが
でき、更に被検体の塗沫作業の高速化を図るこ
とができる。(7) Multiple sets of sample tubes and air nozzles can be arranged, which allows different specimens to be smeared on the same preparation at the same time, further increasing the speed of smearing the specimen. can be achieved.
(8) 被検体の塗沫作業だけに限らず、塗沫作業に
続いて同様のスプレー方式で試薬を塗沫し染色
することもできる。(8) In addition to smearing the specimen, it is also possible to stain the specimen by smearing it with a reagent following the smearing process using a similar spray method.
第１図は本発明に係る塗沫装置の一実施例を示
す要部構成図、第２図は本発明の他の実施例を示
す要部構成図である。
１…移送ベルト、２…プレパラート、３…マス
ク、４…サンプル管、５，２２…毛細管、７…空
気ノズル。
FIG. 1 is a block diagram of main parts showing one embodiment of a smearing device according to the present invention, and FIG. 2 is a block diagram of main parts showing another embodiment of the present invention. 1... Transfer belt, 2... Preparation plate, 3... Mask, 4... Sample tube, 5, 22... Capillary tube, 7... Air nozzle.
Claims (1)
だ毛細管の先端に圧縮空気を噴射し被検体を霧状
に噴出させる空気ノズルと、毛細管より噴射され
る被検体ミストを付着させるためのプレパラート
を載置しこれを間欠的に移送する移送ベルトを備
え、プレパラート上に被検体を噴霧により塗沫し
て被検体標本を作成する塗沫装置において、 プレパラート上に付着した被検体ミストに接触
しない程度の離間間隔をもつてプレパラート上に
着脱可能に取り付けられ、１つまたは複数個の貫
通孔を有し、毛細管より噴射される被検体ミスト
のプレパラート上の塗沫範囲を限定するために使
用されるマスクと、 前記毛細管より被検体を噴射させる際、前記空
気ノズルに与える圧縮空気を所定の強さで一定時
間のあいだ行うように制御する駆動手段と を具備し、プレパラート上に、所定の塗沫範囲に
一定の塗沫厚さで均一に塗沫された被検体標本が
作成されるようにしたことを特徴とする塗沫装
置。[Scope of Claims] 1. An air nozzle that injects compressed air to the tip of a capillary tube containing a sample such as blood or urine by capillary action to eject the sample in the form of a mist, and a sample mist sprayed from the capillary tube. A smear device is equipped with a transfer belt that places a preparation for attachment and transports it intermittently, and creates a specimen specimen by spraying the specimen onto the specimen. It is removably attached to the specimen at a distance that does not touch the specimen mist, has one or more through holes, and limits the area where the specimen mist sprayed from the capillary tube is smeared onto the specimen. and a driving means for controlling compressed air to be applied to the air nozzle at a predetermined strength for a predetermined period of time when the subject is injected from the capillary tube, The smearing device is characterized in that a specimen specimen is prepared by uniformly smearing a smear in a predetermined smearing range with a constant smear thickness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8633280A JPS5712347A (en) | 1980-06-25 | 1980-06-25 | Smearing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8633280A JPS5712347A (en) | 1980-06-25 | 1980-06-25 | Smearing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5712347A JPS5712347A (en) | 1982-01-22 |
| JPS6339869B2 true JPS6339869B2 (en) | 1988-08-08 |
Family
ID=13883876
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8633280A Granted JPS5712347A (en) | 1980-06-25 | 1980-06-25 | Smearing device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5712347A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106988921A (en) * | 2016-01-21 | 2017-07-28 | 丰田自动车株式会社 | The manufacture method of cylinder head |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3948215A4 (en) * | 2019-04-05 | 2022-12-28 | ASP Health Inc. | Consumable components in fluidic sample dispensing systems and methods |
-
1980
- 1980-06-25 JP JP8633280A patent/JPS5712347A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106988921A (en) * | 2016-01-21 | 2017-07-28 | 丰田自动车株式会社 | The manufacture method of cylinder head |
| CN106988921B (en) * | 2016-01-21 | 2019-10-25 | 丰田自动车株式会社 | The manufacturing method of cylinder head |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5712347A (en) | 1982-01-22 |
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