JPS6335412Y2 - - Google Patents

Description

[Detailed description of the invention] This invention is based on the paraffin embedding method for the preparation of microscopic specimens for histopathological examination of collected microscopic tissue pieces such as organ biopsy materials and organ puncture materials. This method ensures and facilitates fixation, dehydration, dealcoholization, paraffin infiltration and embedding from the time of collection of the sample material, and also ensures safety in transporting and transporting the sample material.

For example, in endoscopic examinations of the gastrointestinal tract, the target organ lesion and, if necessary, contiguous organs are observed. Usually, endoscopic findings are recorded in the medical record book. Conventionally, biopsy materials are collected from each necessary location and used for histopathological diagnosis. In some methods, the tissue is adsorbed onto dry filter paper and immediately immersed in formalin fixative, or one container is used for each microscopic tissue piece to ensure identification of the site from which it is collected. Generally, medical institutions perform endoscopic examinations on a large number of patients on the same examination day, which inevitably results in a large amount of test material, which is extremely difficult to process. It has become complicated and requires great care and skill to handle. Furthermore, choosing an easy processing method may result in storing a large number of test materials in the same container.
They are often attached side by side on the same filter paper,
During transportation or transportation, the test material becomes loose or falls off the filter paper, making the sampling site unclear, making it impossible to obtain microscopic findings by sampling site, which had been expected with the utmost care.

Next, when preparing for paraffin embedding, methods such as using commercially available metal mesh baskets or cloth bags or wrapping in gauze are used to prevent tissue fragments from flowing out. In order to ensure that the findings are consistent, it is necessary to have containers that correspond to each of the test materials. Therefore, at the stage of embedding in paraffin, it is very difficult to take out the samples again and rearrange them by patient and sampling site in order, which requires a lot of effort and time. In order to avoid this complication, we use automatic embedding equipment or expose it to excessive dehydration, dealcoholization, and high temperatures without distinguishing it from large tissue pieces such as other surgical materials. Microscopic tissue fragments are subject to significant shrinkage and hardening, which obstructs microscopic examination.

This device not only allows the test material to be safely carried and transported just by performing the operations at the time of collection, but also allows the entire process of embedding in paraffin to proceed without the need to transfer it to another container. At the same time, it is easy to process a large number of patients and a large amount of test material all at once, and it is possible to always obtain microscopic findings that match the sampling site, and to make histopathological diagnosis even easier. It is possible to prepare beautiful microscopic specimens without any damage, and eliminates the need for large quantities of containers and utensils, which eliminates the traditional troubles such as complicated and long preparation work, and the fact that the part from which the sample material to be examined is collected is unclear. We aim to provide something that will eliminate these issues all at once.

An example of its implementation will be described with reference to the drawings. The cage A has a bottom plate 1 with a through hole 6 and a lid 2 with a window 7 that can be opened and closed with a hinge 8. A filter paper 3, a container 4 having a storage chamber 9, and a water passage piece 5 are removably stacked one on top of the other in order, the lid is closed, and the locking piece 10 locks and fixes the container.

The bottom plate 1 is made of a metal material that is corrosion resistant to formalin, a hard or semi-hard synthetic resin such as polyethylene, or a material that is insoluble or poorly soluble in alcohol, acetone, xylene, etc.
A through hole 6 is bored in the center, reinforcing strips are formed along the side edges, fixed frame pieces 11 are erected around the periphery, and the filter paper 3 and container 4 are stacked between the frame pieces 11. Hinge 8 on the proximal end to prevent rattling.
The penetrating shaft bearing 12 is erected, and if necessary, a fork-shaped or arbitrary-shaped push-down protrusion 13 is provided at the distal end, and a protrusion 15 with a suspension hole 14 is provided at the proximal end. Form a protrusion.

The lid body 2 is made of the same material or homogeneous material as the bottom plate 1, and has an appropriate number of windows 7 of arbitrary shape such as round, square, polygon, etc., and has a tip that is fixed to the bottom plate 1. An opening/closing piece 10 which also serves as an elastic member is made to hang down so as to protrude toward the back side of the bottom plate 1, and a frame piece 16 is formed on both sides, and a bearing 12' is provided on the lower surface of the base end side.
An opening/closing piece 18 with a small hole 17 is erected on the top surface, and symbols, characters, etc. can be written on the surface of the opening/closing piece 18 as required.

The filter paper 3 can be attached with a material to be collected and tested, which is well known in this technical field, and when immersed in a processing solution, the processing solution will penetrate through it, and the patient's name and necessary information regarding the material to be tested will be written on the back side. is written with a writing instrument, placed between the frame pieces 11 erected around the bottom plate 1, and viewed through the window 7.

The container 4 is placed on top of the filter paper 3, and like the bottom plate 1 and the lid 2, it is partitioned with metal, synthetic resin, or other material that is corrosion-resistant, insoluble, or poorly soluble in the processing liquid. A suitable number of penetrating containment chambers 9 are provided by drilling holes in the wall itself, and numbers and symbols corresponding to the containment chambers 9 are engraved near the specimens collected inside the containment chambers 9. The microscopic tissue pieces of the material are sequentially accommodated, and the water passage piece 5 is further stacked on the top surface of the container 4, and the lid is closed.

The water-permeable piece 5 is made of a woven or knitted cloth such as gauze, a piece of stainless steel mesh, or a flat plate with micropores, which has so-called water-permeable properties through which the treatment liquid can penetrate, and is coated over the container containing the test material. urge

The present invention has the above-mentioned configuration, and to explain its usage condition, it is used in preparation for collection of extremely small tissue pieces of organs. A processing solution such as physiological saline or formalin fixative is injected into the container, and with the lid open, the filter paper 3 and container 4 with necessary information written on them are placed on the bottom plate 1, and the frame piece 11 is used to hold them. It is fixed and immersed in the treatment solution.

Thereafter, the collected microscopic tissue pieces of the test material are sequentially accommodated in the accommodation chamber of the container. After the necessary collection is completed, cover the container 4 with the water passage piece 5, gently lift the holder A, close the lid 2, and elastically lock and fix it to the bottom plate 1 with the locking piece 10. . At this time, water passage piece 5
is in close contact with the upper surface of the container 4 without being displaced laterally by the frame piece 16 of the lid 2. After these steps, the tissue piece is fixed in formalin by immersing it in a sufficient amount of a processing solution such as formalin fixative. The formalin fixative solution sufficiently penetrates into the tissue pieces in the storage chamber 9 of the container 4 through the window 7 of the lid 2, the water passage piece 5, the through hole 6 of the bottom plate 1, and the filter paper 3. At this stage, carrying and transporting to a laboratory etc. becomes optional, and all operations necessary for subsequent embedding in paraffin, including washing with water, can be accomplished by simply grabbing and moving holder A with tweezers. I can do it.
Infiltration of paraffin into microscopic tissue pieces is sufficient by embedding them one after another in paraffin that has previously been melted and kept warm. When the sample material of the extremely small tissue piece is to be taken out at the stage where dealcoholization is completed, the holder A is taken out and the bottom plate 1 is placed on a table with the tip side floating by the locking piece 10.
After that, when the push-down protrusion 13 provided on the bottom plate 1 is pushed down with tweezers or the like, the lid 2 is lifted up using the locking piece 10 as a fulcrum, the locking is released, and the opening/closing piece 11 is lowered to raise the lid 2 and open the lid. do.

Thus, the tiny tissue pieces can be freely removed, completing the entire operation from collection to paraffin embedding.

In this invention, a holder is constructed by attaching a windowed lid body to a bottom plate with a through hole so that it can be opened and closed, and a container having a filter paper and a storage chamber and a water passage piece can be attached and detached in sequence to the holder. It is fixed from the time of collecting the test material because it is stacked on top of the
Dehydration, dealcoholization, paraffin infiltration and embedding can be carried out reliably and easily, and test materials can be carried and transferred safely. Furthermore, even if there are a large number of test materials, they can be easily processed without being mixed up, and the test materials will not become loose or fall off from the filter paper during transportation or transportation. Furthermore, when embedding in paraffin, there is no need to rearrange or transfer the sampled test materials again, and the time and complexity can be omitted and they can be easily processed using an automatic embedding device, allowing for the desired results. This objective can be easily achieved.

[Brief explanation of the drawing]

The drawings show one embodiment of the container of the present invention, and Fig. 1 is a front view, Fig. 2 is a plan view, Fig. 3 is a bottom view, and Figs. 4 and 5 are a right side view and a left side view. figure,
FIG. 6 is an exploded perspective view, and FIG. 7 is an explanatory view with the lid opened, with a portion cut away. In the figure, A is a holder, 1 is a bottom plate, 2 is a lid, 3 is a filter paper, 4 is a container, 5 is a water passage piece, 6 is a through hole, 7 is a window, and 9 is a storage chamber.

Claims (1)

[Scope of utility model registration request] A holder is constructed by attaching a windowed lid body to a bottom plate with a through hole so that it can be opened and closed, and a container having a filter paper and a storage chamber, and a water passage piece are successively stacked on the holder in a detachable manner. Container for extremely small tissue pieces.