JPS63302959A - Breaking processing apparatus - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to a crushing device for easily crushing or damaging suspended matter in a suspension or solid powdery particulate matter.

Conventional technology Conventionally, as a device for crushing or damaging animal and plant tissues and microbial cells to obtain useful substances inside the cells, there are hammer-set crushers and rotary crushers that apply mechanical energy such as impact and crushing to fine powder. Machines, ball mills, jet mills, etc. are used.

Problems to be Solved by the Invention However, since these conventional crushing and damaging methods involve heat generation due to mechanical energy, crushing load in the field of biotechnology that deals with animal and plant tissues, microbial cells, etc. It wasn't useful.

In addition, in the hammer set pulverizer or jet mill, the pulverized material is likely to scatter during pulverization.

In cases where biohazard measures such as genetic manipulation are required, there is a risk of biological contamination.

Means for Solving the Problems The present invention aims to minimize such biological contamination while damaging animal and plant tissues and microbial cells, and removing enzymes, antibiotics, peptides, or protein-based bioactive substances within the cells. The purpose is to provide a device that can facilitate the recovery of useful substances, and the purpose is to provide a device that includes a driving roller that rotates around a central axis and a driven roller that rotates around an eccentric rotating shaft. A roller and a means for pressing the driven roller against the driving roller in conjunction with the driven roller are provided in the casing, and a sample supply port is provided above the contact point between the driving roller and the driven roller. Achieved by breakage processing equipment.

Main Wall: In the device of the present invention, the driven roller rotates around an eccentric rotating shaft while being pressed against and constantly in contact with the driving roller, so that the contact point of both rollers constantly moves up and down during the rotation.

Therefore, the target animal and plant tissues and microorganisms suspended in a suspended liquid or solid powder granular materials containing animal and plant tissues and microorganisms are continuously caught between the driving roller and the driven roller,
Moreover, it is compressed between both rollers and crushed or damaged.

An example of undeveloped IJJ will be described with reference to the drawings.

In FIG. 1, l is a stainless steel box-shaped casing having an outlet on the side from which the collection container 20 can be taken out;
and 3 are a cylindrical driving roller and a driven roller made of stainless steel, respectively.

The drive Hl roller 2 has a central shaft 4 firmly fixed thereto.
It is equipped with The central shaft 4 is solidly concentric with the driving roller 2, and is connected to a driving motor 5 shown in FIG.
Therefore, the driving roller 2 is connected to the central shaft 4
Rotate around the center in the direction of the arrow (shown in Figure 1).

The driven roller 3 is provided with an eccentric shaft 7 that is eccentric from the center 6 of the rotation shaft 21 by a distance e and formed integrally with the rotation shaft 21. Further, the rotating shaft 21 is arranged so that its shaft center 6 is located below and closer to the center of the driving roller.

The eccentric shaft 7 is provided concentrically with respect to the driven roller 3 and is fixed to the driven roller 3.

As best shown in FIG. 2, the rotating shaft 21 is supported by bearings and is allowed to rotate freely.

Therefore, the driven roller 3 is moved at a distance 111e from its center 7.
The rotating shaft 21 rotates around the center 6 of the rotating shaft 21, which is eccentric by a certain amount.

As is clear from FIG. 3, the eccentric shaft 7 is connected to one end of the arm 8, and the pressing shaft 11 is slidably inserted into the opening lO provided at the other end of the arm 8. . The suppression axis 11 is stage al! The handle 14 becomes thicker in the middle of the casing 12 and extends to the outside of the casing 1, and a handle 14 is fixed to the end thereof. A side surface 13 of the pressing shaft 11 between the stepped portion 12 and the handle 14 is adapted to be screwed into a nut 22 fixed to the side surface of the casing l.

A spring 9 is inserted between the arm 8 and the stepped portion 12 of the pressing shaft 11, and the driven roller 3
The elasticity of the spring 9 keeps the driven roller 3 pressed against the driving roller 2 at all times, so that the crushing process can be carried out efficiently. be.

Further, an opening 17 is provided on the outer surface of the casing 1 on one side of the contact point 1 between the driving roller 2 and the driven roller 3.
6 can be inserted. The sample supply q 16 may be a dropping type droplet as shown in the figure, or the sample may be ejected near the contact point of the roller using the gas flow rate of an inert high-pressure gas such as nitrogen gas or CO2 gas. It is also possible to adopt a configuration in which an L stage such as an injection nozzle is installed.

Hereinafter, the operation of this embodiment will be explained using as an example a case in which a suspension of microorganisms in a suspension or a suspension of animal and plant tissue is used as a sample. Needless to say, the same applies to pulverization.

Supply test t' [1] Suspension drop port 16 to casing 11:lrl! A sample container 15 is placed on the upper surface of the casing 1 so as to be inserted into an opening 17 provided in the casing 1.

Note that the suspension container 15 has a valve handle 18 provided on the lid.
The amount of the suspension 19 dripping from the suspension drop port 16 can be adjusted by this.

Then, while dropping the suspension 19 from the suspension droplet 16, the drive motor 5 is turned on to rotate the drive roller 2. Then, since the driven roller 3 is always pressed against the driving roller 2 by the spring 9 provided on the pressing shaft 11, the rotation axis 21 is rotated around the center 6 of the rotating shaft 21, which is eccentric by a distance e from the center 7 of the driven roller 3. Rotate to .

In this way, since the driven roller 3 rotates around the eccentric point 6, the point of contact with the driving roller 2 moves up and down. Therefore, as the contact point moves, the dropped suspension 19 is caught between both rollers 2.3, and as the contact point moves, the suspended matter on both rollers 2.3 Collection container 2 that has been compressed and crushed and placed on the bottom inside the casing 1
It is recovered to 0.

It goes without saying that by adjusting the pressure between the rollers 2 and 3 using the handle 14, the degree of processing of the cells by the above-mentioned squeezing and pulverizing process can be adjusted depending on the type of suspended matter, ie, microbial cells, etc.

In addition to the configuration shown in the embodiment, there is one driven roller that rotates around the eccentric rotation axis in the device of the present invention.
In the devices shown in FIGS. 3 to 3, directly, without using the eccentric shaft 7,
The rotating shaft 21 may be fixed at a position eccentric from the center of the driving roller 3, or the rotating shaft 21 may be fixed to the casing 1 and the rotating shaft 21 may be eccentric from the center of the driven roller 3 and connected directly. However, it is also possible to configure the driven roller 3 to be able to rotate with respect to the rotating shaft 21. In other words, the presence of the driven roller allows the contact points of both rollers 2.3 to be vertically moved. Needless to say, it includes all configurations that perform the function of doing so.

Further, the cylindrical surfaces of the driving roller 2 and the driven roller 3 may be made smooth or rough, and either one of the driving roller 2' and the driven roller 3 having a circular cross section as shown in FIGS. 1 to 3, or Both oval cross section or involute, cycloid,
The driven roller 3 may be eccentrically rotated as a curved cross section using a trochoid curve, or may be made of other material, and the pressing rod 1 may be equipped with a spring 12.
1. The above-mentioned pressing means consisting of 1 may be replaced by other means such as hydraulic pressure, or when frozen particles of a suspension are used as a sample, a refrigerant may be introduced into the casing, or a cooling jacket may be provided on the casing, etc. It goes without saying that various modifications and alterations can be made within the scope of the idea of the invention.

Effects of the Invention As detailed above, the present invention provides an apparatus that can damage animal and plant tissues and microbial cells while minimizing biological contamination and facilitate the recovery of useful substances. be able to.

[Brief explanation of the drawing]

FIG. 1 is a schematic front view showing the internal structure of an apparatus according to the present invention, FIG. 2 is a schematic side view of the apparatus of FIG. -z′ sectional view. Also, in the drawing. l...Casing, 2...Drive roller, 3...
... Driven roller 4 ... Center shaft, 5 ... Drive motor, 6 ... Rotating shaft center, 7 ... Eccentric shaft,
8... Arm, 9... Spring, lO... Opening, 11... Press shaft, 12... Step part, 13...
... Threaded portion, 14... Handle, 15... Sample container, 16... Sample supply port, 17... Opening,
18... Valve handle, 19... Sample,
20... Recovery container, 21... Rotating shaft. Agent Masao Miyake and 1 other person Figure 1 Figure 2 Figure 2.

Claims (2)

[Claims] (1) A driving roller 2 that rotates around a central axis 4, a driven roller 3 that rotates around an eccentric rotating shaft 6, and a driving roller 2 that moves the driven roller 3 in conjunction with the driven roller 3. A breakage processing device comprising means 8 to 15 for pressing the casing, and further comprising a sample supply port 16 above the point of contact between the driving roller and the driven roller. (2) The apparatus according to claim 1, wherein the sample supply port 16 has a jetting means for jetting the specimen toward the contact point between the driving roller 2 and the driven roller 3.