JPS63291572A - Culture of microorganism - Google Patents
Culture of microorganismInfo
- Publication number
- JPS63291572A JPS63291572A JP12369887A JP12369887A JPS63291572A JP S63291572 A JPS63291572 A JP S63291572A JP 12369887 A JP12369887 A JP 12369887A JP 12369887 A JP12369887 A JP 12369887A JP S63291572 A JPS63291572 A JP S63291572A
- Authority
- JP
- Japan
- Prior art keywords
- carbon dioxide
- microorganisms
- dioxide gas
- microbial
- alkaline solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 18
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 24
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 24
- 238000010521 absorption reaction Methods 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 230000000813 microbial effect Effects 0.000 claims abstract description 8
- 239000012670 alkaline solution Substances 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims abstract description 6
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims abstract description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 4
- 235000015097 nutrients Nutrition 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 11
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 3
- 238000012136 culture method Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 16
- 238000000855 fermentation Methods 0.000 description 12
- 230000004151 fermentation Effects 0.000 description 12
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 6
- 239000001630 malic acid Substances 0.000 description 6
- 235000011090 malic acid Nutrition 0.000 description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 241000222480 Schizophyllum Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000019992 sake Nutrition 0.000 description 1
- 235000020083 shōchū Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000015041 whisky Nutrition 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、大気中に放出されている炭酸ガスを微生物に
取り込ませ、より有効な物質に転換することを目的とす
るものである。DETAILED DESCRIPTION OF THE INVENTION The object of the present invention is to allow microorganisms to take in carbon dioxide gas released into the atmosphere and convert it into a more effective substance.
ビール・清酒・焼酎・ウィスキー・ワイン等の醸造その
池、微生物が関与する各種製造工程、燃焼・反応等の微
生物が関与しない工程から発生する炭酸ガスは、ごく一
部利用されているのみであり、殆ど利用されないで、大
気中に放出されているのが現状であり、しげんの有効利
用、大気圏の空気組成の変化の防止等の面からも、炭酸
ガスの利用が望まれるところである。Only a small portion of the carbon dioxide gas generated from the brewing of beer, sake, shochu, whiskey, wine, etc., various manufacturing processes involving microorganisms, and processes that do not involve microorganisms such as combustion and reactions is used. Currently, carbon dioxide is hardly used and is released into the atmosphere, and the use of carbon dioxide is desired from the viewpoint of effective use of carbon dioxide and prevention of changes in the air composition of the atmosphere.
微生物が関与する工程、微生物が関与しない工程と微生
物が関与する工程との混合系から発生する炭酸ガスをふ
くむガスを、アルカリ水溶液中（例えば、水酸化ナトリ
ウム・水酸化カリウム・水酸化カルシウム等）に導き吸
収させ、炭酸塩溶液を得る。Gas containing carbon dioxide generated from a process involving microorganisms, a process not involving microorganisms, and a process involving microorganisms in an alkaline aqueous solution (e.g., sodium hydroxide, potassium hydroxide, calcium hydroxide, etc.) to obtain a carbonate solution.
炭酸ガスを吸収した吸収液を、そのままあるいは、適当
に希釈したのも、炭酸塩利用可能な微生物１こ、炭酸源
あるいは炭素源とし一乙池の栄養源と混合あるいは単独
で、供給あるいは添加し、微生物を増殖あるいは微生物
を介して得られる菌体あるいは生成物を利用するもので
ある。The absorption liquid that has absorbed carbon dioxide gas can be supplied or added as it is or diluted appropriately, or mixed or alone with one microorganism that can utilize carbonate, or as a carbon source or a nutrient source of Ichioto Pond. This method utilizes microbial cells or products obtained by growing microorganisms or through microorganisms.
（実施例１）
炭酸ガスボンベから炭酸ガスを図−１に示す装置に導き
２％水酸化ナトリウム溶液に５００ｍ１／ｍｉｎで通気
吸収させ、ｐＨｌｏ、１５の吸収液を得た。(Example 1) Carbon dioxide gas was introduced from a carbon dioxide gas cylinder into the apparatus shown in Figure 1, and was aerated and absorbed into a 2% sodium hydroxide solution at a rate of 500 ml/min to obtain an absorption liquid with a pHlo of 15.
図−２に示す三相流動層タイプの連続発酵槽に、スエヒ
ロタケ（学名Ｓｃｈｉｚｏｐｈｙｌｌｕｍ Ｃｏｍｍｕ
ｎｅ ＩＦＯ４９２８）を固定化し、装置下部より吸収
液を含む培地を、１日に２５０ＭＬづつ連続的に供給す
ると共に、通気を行い、ｐｉ！ ４ 、５〜６．５でリ
ンゴ酸発酵を行わせた。In the three-phase fluidized bed type continuous fermentation tank shown in Figure 2, Schizophyllum commu (scientific name: Schizophyllum commu) was grown.
ne IFO4928) was immobilized, a culture medium containing an absorption liquid was continuously supplied from the bottom of the device at a rate of 250 ML per day, and aeration was performed. 4. Malic acid fermentation was carried out at 5 to 6.5.
溢流してきた発酵液中に含まれるリンゴ酸の濃度は表−
１のとおりである。The concentration of malic acid contained in the overflowing fermentation liquid is shown in the table below.
As per 1.
（実施例２）
白米１２００ｋｇを仕込んだ清酒の発酵タンクより発生
する炭酸ガスを含むガスを図−１に示す装置に導き、８
％水酸化す） ＋７ウム溶液に５００ｍ１／ｍｉｎで通
気吸収させ、ａｌｌｌＯ，５０の吸収液を得た。(Example 2) Gas containing carbon dioxide generated from a sake fermentation tank containing 1200 kg of polished rice was introduced into the apparatus shown in Figure 1, and 8
% hydroxide) was aerated and absorbed at a rate of 500 ml/min to obtain an absorption liquid of allO.50.
図−２に示す三相流動層タイプの連続発酵層に、固定化
したスエヒロタケ （学名５ｃｈｉｚｏｐｈｙｌ ｔｕ
ｒｎＣｏｍ＋ｏｕｎｅ ＩＦＯ４９２８）をいれ、装置
下部より４倍に希釈した吸収液を含む培地を、１日に２
５０ＭＬづつ連続的に供給すると共に、通気を行い、ｐ
Ｈ４，５〜６．５でリンゴ酸発酵を行わせた。Chizophyl tu immobilized in a three-phase fluidized bed type continuous fermentation bed shown in Figure 2.
rnCom+one IFO4928) and add the medium containing the absorption liquid diluted 4 times from the bottom of the device twice a day.
Continuously supply 50 ML at a time, aerate, and p
Malic acid fermentation was carried out at H4.5-6.5.
溢流してきた発酵液中に生成されてきたリンゴ酸の濃度
は、表−２のとおりである。The concentration of malic acid produced in the overflowing fermentation liquor is shown in Table 2.
この発酵液中のリンゴ酸をイオン交換樹脂により回収精
製した結果は、表−３のとおりである。Table 3 shows the results of recovering and purifying malic acid in this fermentation liquid using an ion exchange resin.
ｐｉ−１ｆ’ｅｓｕｌｔｓ ｏｆ ＣＯｚ−ｆｉｘｉｎ
ｇ ｆｅｒｍ＠ｎｔａｔｉｏｎ ｗｉｔｈ Ｃｏｗａｂ
ｓｏｒｂ＠ｄ ５ｏｌｕｔｉｏｎ （ｆｒｏｒａＣｏｔ
−Ｂｏｍｂ）表−２Ｒｅ５ｕｌｔｓ ｏｆ Ｃｏ２
−ずｉｘｉｎｇ ｆｅｒｍｅｎｔａｔｌｏｎ ｗｉ
ｔｈ ＣＯ２−。pi-1f'esults of COz-fixin
g ferm@ntation with Cowab
sorb@d 5solution (floraCot
-Bomb) Table-2Re5ults of Co2
-zuixing fermentatlon wi
th CO2-.
ａｂ＠ｏｒｂｅｄ ＠ｏｌｕｔｉｏｎ （ｆｒｏｍ Ｓ
ａｋｅ−Ｍｏｒｏｍｉ）４ 図の簡単な説明
図−１
炭酸ガスを吸収させるための装置で、３つの吸収槽を持
ち、第１、第２槽は、ラシヒリングを充填した吸収槽で
吸収効率を高めるものである。ab@orbed @solution (from S
ake-Moromi) 4 Simple explanatory diagram of figure-1 A device for absorbing carbon dioxide gas, which has three absorption tanks, the first and second tanks are filled with Raschig rings to increase absorption efficiency. It is.
炭酸ガスを含む気体が、コンプレッサーを通って第１吸
収槽に導かれ、上部からアルカリ液が供給され、向流接
触により炭酸ガスが吸収される。Gas containing carbon dioxide gas is led to the first absorption tank through a compressor, an alkaline solution is supplied from the upper part, and carbon dioxide gas is absorbed by countercurrent contact.
炭酸ガスを吸収したアルカリ液と未吸収の炭酸ガスを含
む気体は、第２吸収槽でさらに炭酸ガスを吸収し、吸収
液とガスが第３槽に送られ、さらに吸収したのちに、貯
槽に入る。吸収されなかったガスは貯槽上部より放出さ
れる。吸収液はまた第１吸収槽に再循環させ、目的とす
る量の炭酸ガスを吸収するまで何度でも循環させ、炭酸
ガス吸収液を得るものである。The alkaline liquid that has absorbed carbon dioxide gas and the gas containing unabsorbed carbon dioxide further absorb carbon dioxide gas in the second absorption tank, and the absorption liquid and gas are sent to the third tank, and after further absorption, are transferred to the storage tank. enter. Unabsorbed gas is released from the top of the storage tank. The absorption liquid is also recirculated to the first absorption tank, and the circulation is repeated as many times as necessary until a desired amount of carbon dioxide is absorbed, thereby obtaining a carbon dioxide absorption liquid.
図−２
リンゴ酸発酵を連続的にさせるだめの３層からなる装置
で、内部には固定化菌体をいれ、装置下部から炭酸ガス
吸収液を含む培地を、連続的に供給すると共に空気を吹
込む。発酵中のｐＨを一定にフントロールするため、第
２層でｐＨメーターによりｐＨを測定し、第３層でｐＨ
が一定になるように、酸あるいはアルカリを添加してい
る。また、発酵中に発泡することもも考えられるので、
消泡剤を適宜添加する。また、発酵槽内の溶存酸素量も
測定が出来るように溶存酸素計が装着しである。Figure 2: A device consisting of three layers that allows malic acid fermentation to occur continuously.The inside contains immobilized bacterial cells, and a medium containing a carbon dioxide absorption solution is continuously supplied from the bottom of the device, while air is also pumped out. Infuse. In order to maintain a constant pH during fermentation, the pH is measured using a pH meter in the second layer, and the pH is measured in the third layer.
An acid or alkali is added to keep the amount constant. Also, foaming may occur during fermentation, so
Add antifoaming agent as appropriate. Additionally, a dissolved oxygen meter is installed so that the amount of dissolved oxygen in the fermenter can be measured.
上部よりオーバーフローしてきた発酵液を生成物として
得るものである。The fermentation liquid that overflows from the top is obtained as a product.
Claims (4)
物が関与しない系との混合系から発生する炭酸ガスを、
アルカリ性の溶液に通気吸収させ、炭酸塩溶液を得る方
法(1) Carbon dioxide gas generated from a system involving microorganisms, or a mixed system of a system involving microorganisms and a system not involving microorganisms,
Method of obtaining carbonate solution by aeration and absorption in alkaline solution
源、あるいは連続式培養法の培地として、他の栄養源等
と混合、あるいは単独に供給する方法(2) A method in which the absorption liquid obtained by the method in (1) is mixed with other nutrients, etc., or supplied alone as a carbon source for microorganisms or as a medium for continuous culture.
菌体、あるいは分散した菌体いずれも含むものである。(3) The form of microorganisms obtained by the method (2) above includes both immobilized microbial cells and dispersed microbial cells.
て得られた菌体、あるいは菌体の生産物を利用する方法(4) A method of using bacterial cells or bacterial cell products obtained by culturing them by methods (1), (2), and (3) above.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12369887A JPS63291572A (en) | 1987-05-22 | 1987-05-22 | Culture of microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12369887A JPS63291572A (en) | 1987-05-22 | 1987-05-22 | Culture of microorganism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS63291572A true JPS63291572A (en) | 1988-11-29 |
Family
ID=14867122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12369887A Pending JPS63291572A (en) | 1987-05-22 | 1987-05-22 | Culture of microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63291572A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2004074191A1 (en) * | 2003-02-21 | 2006-06-01 | 栗田工業株式会社 | Ammonia nitrogen-containing water treatment method |
-
1987
- 1987-05-22 JP JP12369887A patent/JPS63291572A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| J.BREW.SOC.JAPAN=1985 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2004074191A1 (en) * | 2003-02-21 | 2006-06-01 | 栗田工業株式会社 | Ammonia nitrogen-containing water treatment method |
| JP4735256B2 (en) * | 2003-02-21 | 2011-07-27 | 栗田工業株式会社 | Ammonia nitrogen-containing water treatment method |
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