JPS63290572A - Medical material and treatment of wound using the same - Google Patents

Abstract

PURPOSE:To enhance the close adhesiveness to the tissue of a living body, by mixing stable fibers with gelatin or collagen and crosslinking the resulting mixture. CONSTITUTION:Staple fibers are mixed with gelatin or collagen and the resulting mixture is crosslinked to form a medical material. The jelly strength of gelatin or collagen is about 100 bloom or more and especially pref. about 150-300 bloom. The modification of gelatin or collagen is performed by acid or alkali treatment but the modification due to alkali treatment is pref. As the fiber, nylon, polyester, polyimide, cellulose, polyvinyl alcohol, acrylonitrile or a modified one thereof are used.

Description

Detailed Description of the Invention [Industrial Application Field] The present invention relates to a medical material, more specifically, a medical material having high adhesion to living tissues, which is made by mixing gelatin or collagen with short fibers and crosslinking the mixture. The present invention relates to methods for treating wounds using them.

[Prior Art] Gelatin and collagen have excellent biocompatibility and have been used as medical materials to be implanted in living bodies or as covering materials.

In addition, when using these, in order to maintain the flexibility and physical strength of gelatin or collagen, crosslinking is performed with aldehydes, isocyanates, glycidyl ethers, etc.
These crosslinked gelatins or crosslinked collagens are used in wound covering materials, artificial blood vessels, anti-adhesion membranes, hemostatic agents, artificial ureters, and the like. (For example, see Japanese Patent Publication Nos. 31-4644 and 40-9431.) [Problems to be Solved by the Invention] However, when these crosslinked gelatins or crosslinked collagens are used for the above purposes, especially as wound covering materials, The current situation is insufficient. This is because, although it is desirable for the material to have high adhesion to the wound surface in order to speed up the healing effect of the wound, it is difficult to say that the material has sufficient adhesion.

[Means for Solving the Problems] Therefore, as a result of intensive research to solve the problems in the prior art, the present inventors discovered a medical material with high IE adhesion to living tissue, and the present invention has been reached.

That is, according to the present invention, a medical material characterized in that it is made by mixing gelatin or collagen with short fibers and crosslinking the mixture,
and a method for treating a wound, which is characterized by using a medical material made by mixing gelatin or collagen with short ia male and crosslinking the mixture.

In the present invention, gelatin and collagen commonly used for medical purposes can be used as they are or after being modified. The gelatin and collagen preferably have a jelly strength of 100 Bloom or more, particularly 150 to 300 Bloom. Further, gelatin and collagen are denatured by acid treatment or alkali treatment, and denaturation by alkali treatment is preferred.

Further, specific examples of the fibers used in the present invention include nylon, polyester, polyimide, cellulose, polyvinyl alcohol, acrylonitrile, or modified products thereof, preferably nylon and polyester, or are limited to these. Alternatively, one or more of these, or a mixture thereof may be used.

The thickness of the fibers to be mixed with gelatin or collagen is not particularly limited, and is usually 0.5 μm to 70 μm, preferably 5 μm to 40 μm. When the fibers are mixed with gelatin or collagen, it is preferable that they are mixed as uniformly as possible, so the length of the fibers is usually 1-10 mm.
However, it is not limited to this range. The fibers may be straight, bent or twisted.

When gelatin or collagen is used as a medical material, it is crosslinked to improve its flexibility and strength. As the crosslinking agent, the details will be described later, or aldehydes, glycidyl ethers, isocyanates, etc. can be used.

Examples of the above aldehydes include formaldehyde, glyoxal, and glutaraldehyde.

Examples of glycidyl ethers include ethylene propylene glycol diglycidyl ether, glycerol polyglycidyl ether, diglycerol polyglycidyl ether, polyglycerol polyglycidyl ether, sorbitol polyglycidyl ether, and ethylene glycol diglycidyl ether. In order to obtain efficiently, ethylene glycol diglycidyl ether is preferable.

Isocyanates include difunctional ones such as hexamethylene diisocyanate, tetramethylene diisocyanate, o-tolidine isocyanate, tolylene diisocyanate, naphthylene-1,5-diisocyanate, 4,4'-diphenylmethane diisocyanate, and triphenylmethane-4 , 4', 4''-)-diisocyanate and other polyfunctional residues.

The mixing ratio of gelatin or collagen and fibers mixed therein is usually 100:0.01 to 100:lO (weight ratio), preferably 100:0.1 to 100:l (weight ratio)
It is.

When producing the medical material of the present invention in which gelatin or collagen is mixed with fibers and crosslinked, a foam may be prepared using, for example, sodium lauryl sulfate, coconut oil fatty acid collagen peptide, and its salts.

The medical material of the present invention, which is made by mixing gelatin or collagen with short fibers and crosslinking the mixture, and which has high adhesion to living tissue, can be produced, for example, as follows.

After leaving a mixture of gelatin or collagen and water at room temperature, the gelatin temperature is 30 to 70°C, preferably 30°C.
The gelatin or collagen is dissolved by gentle stirring at 5-45°C, and at 20-40°C for collagen. The short fibers, crosslinking agent and blowing agent are then added while still maintaining the same temperature, and the mixture is usually stirred and allowed to react.

The amount of crosslinking agent used varies depending on its type, and is usually 0.01 to 10 mm01e groups/g gelatin.

In addition, the blowing agent is usually o, oos ~ 10 to gelatin.
Use weight %.

There are no particular restrictions on the tank or stirring blade used for the reaction, but when a foaming agent is used, it is preferable to use a stirring blade with a large stirring effect to stir at high speed. Insufficient crosslinking has the disadvantage that it easily dissolves in blood and body fluids.

In particular, when used as a wound dressing, it is preferably a foam.

That is, a wound covering material can be obtained by injecting the gelatin foam prepared by high-speed stirring as described above into a molding machine according to a conventional method, freeze-drying it, and slicing it.

The gelatin or collagen produced in this manner by mixing short fibers and crosslinking has far superior adhesion to living tissue compared to crosslinked gelatin or collagen without short fibers, and it also has a wound healing effect. early.

The medical material of the present invention includes fibronectin, lysozyme,
Add and absorb pladiomycin, gatamycin, polymyxin, urokinase, TPA, SOD, etc.
Alternatively, these can be supported and used.

The medical material of the present invention can also be used as a composite consisting of a film-like or mesh-like synthetic polymer material layer. When used as a wound covering material, if it is not a composite, it can be used by applying it to the wound site by an appropriate method and covering the outer surface with gauze or the like.

The synthetic polymeric material used to form the composite has a moderate water vapor permeability and oxygen permeability, e.g.
Those with a moisture permeation rate in the range of m g/cm ”/hr can be used.It also has an infection prevention effect that prevents the invasion of bacteria from the outside, and also has an effect of preventing the outflow of body fluids. It is desirable that the

[Examples] Hereinafter, the present invention will be explained in more detail based on Examples.
It will be clear that the invention is not limited to these examples.

(Example 1) Preparation of gelatin 200 mM of distilled water was added to 30 g of gelatin, and the mixture was left at room temperature for 6 hours, then heated to 40° C. and stirred to dissolve. Then,
2.40 g of ethylene glycol diglycyl ether as a crosslinking agent, 1 gram of sodium lauryl sulfate as a blowing agent
．． 0g and thickness 30 pots, length 5mm Q) Add 0.10g of nylon fiber 1iiiI, 18000 r, p-
The mixture was stirred for 2 minutes at a rotational speed of m. The foamed viscous material with dispersed short fibers was quickly poured into a mold with dimensions of 50 mm x 50 mm x 5 mm and subsequently rapidly freeze-dried.

The resulting gelatin foam was sliced to a thickness of 0.4 mm. The resulting short fiber-dispersed gelatin foam was heated at 37°C for 2 hours.
Although it was immersed in physiological saline for 4 hours, no weight loss was observed, indicating that no elution occurred.

Evaluation of Wound Healing The IWister strain male rats (9 weeks old, body weight 30
After removing hair (0 to 350 g) from the back and anesthesia, a 2.5 cm x 5 cm wound was made on the back using a dermatome. The wound covering material of the example was applied to the wound site, and the wound surface was observed after 3.7.10.14.17.21, and changes in the wound covering material itself were observed.

The results are shown in Table 1.

Evaluation of wound healing effect (Part 2) A glass rod with a diameter of 2 cm at the tip was immersed in physiological saline at 80°C, and after the temperature of the glass rod itself had risen to 80°C, it was taken out and quickly injected into the above rats. 1 on the back where hair has been removed
It was pressed for 0 seconds. The blisters generated at the burn site were peeled off after 2 hours, a wound covering material was attached to the burn surface, and the same observations as above were made. The results are shown in Table 1.

(Example 2) A gelatin foam was produced in the same manner as in Example 1 except that 0.20 g of polyester fibers with a thickness of 10 g and a length of 10 mm were added instead of nylon. The resulting gelatin foam was
Although it was immersed in physiological saline at 7°C for 24 hours, no weight loss was observed, indicating that it did not elute. Using this polyester fiber-dispersed gelatin foam, the wound healing effect was evaluated in the same manner as in Example 1. The results are shown in Table 1.

(Comparative Example) Slices of gelatin foam were produced in the same manner as in Example 1 except that nylon fibers were not added. The wound healing effect of the sliced gelatin foam was evaluated in the same manner as in Example 1.

The results are shown in Table 1.

In addition, in Table 1, in order to compare the wound healing effects, in addition to the evaluation results of Examples and Comparative Examples, the results of natural healing of wounds are also listed.

(Hereinafter, blank space) [Effects of the Invention] As explained above, the medical material of the present invention has excellent adhesion to living tissues, so by treating wounds with it, it is possible to effectively heal wounds. It can be cured.

Claims (4)

[Claims] (1) A medical material characterized by being made by mixing gelatin or collagen with short fibers and crosslinking the mixture. (2) The medical material according to claim 1, wherein the short fibers are nylon or polyester. (3) The medical material according to claim 1, wherein the medical material is a wound covering material. (4) A wound treatment method characterized by using a medical material made by mixing gelatin or collagen with short fibers and crosslinking the mixture.