JPS63212853A - Electrophoretic portionwise sampling apparatus - Google Patents
Electrophoretic portionwise sampling apparatusInfo
- Publication number
- JPS63212853A JPS63212853A JP62046109A JP4610987A JPS63212853A JP S63212853 A JPS63212853 A JP S63212853A JP 62046109 A JP62046109 A JP 62046109A JP 4610987 A JP4610987 A JP 4610987A JP S63212853 A JPS63212853 A JP S63212853A
- Authority
- JP
- Japan
- Prior art keywords
- migration
- pipe
- electrophoresis
- specimen
- take
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005070 sampling Methods 0.000 title abstract description 3
- 238000001962 electrophoresis Methods 0.000 claims abstract description 25
- 238000000605 extraction Methods 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 7
- 230000005012 migration Effects 0.000 abstract description 24
- 238000013508 migration Methods 0.000 abstract description 24
- 230000003287 optical effect Effects 0.000 abstract description 10
- 238000005194 fractionation Methods 0.000 abstract description 6
- 239000003480 eluent Substances 0.000 abstract 7
- 239000000523 sample Substances 0.000 description 24
- 238000001514 detection method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000008151 electrolyte solution Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000000945 filler Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 101100204059 Caenorhabditis elegans trap-2 gene Proteins 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は蛋白質などの生体試料を電気泳動を用いて分離
し、分取する装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to an apparatus for separating and fractionating biological samples such as proteins using electrophoresis.
（従来の技術） 第３図に自由電気泳動を用いた分取装置を示す。(Conventional technology) Figure 3 shows a fractionation apparatus using free electrophoresis.
２は泳動部であり、例えば、２枚のガラス板の０．５ｍ
ｍ程度の隙間に電解液が充填されたものである。泳動部
２は垂直方向に設置され、一方の端辺には＋側サイド電
極４が設けられ、他方の端辺には一側サイド電極６が設
けられている。2 is an electrophoresis section, for example, a 0.5m section of two glass plates.
An electrolytic solution is filled into a gap of about 1.5 m. The migration section 2 is installed in a vertical direction, and a positive side electrode 4 is provided on one end side, and a one side side electrode 6 is provided on the other end side.
試料８は平板泳動部２の上部の中央部に導入される。試
料８が下方に移動中、両サイド電極４゜６により電場が
かけられ、試料８は矢印で示されるように下方に移動し
つつ左右に分離される。このように試料８が分子量と帯
電に応じて分離され、泳動部２の下端からトラップ１２
−１〜１２−ｎに分取される。The sample 8 is introduced into the upper center of the plate migration section 2 . While the sample 8 is moving downward, an electric field is applied by both side electrodes 4.about.6, and the sample 8 is separated into left and right sides while moving downward as shown by the arrows. In this way, the sample 8 is separated according to its molecular weight and charge, and the sample 8 is separated from the lower end of the electrophoresis section 2 to the trap 12.
-1 to 12-n.
（発明が解決しようとする問題点）
第３図に示される分取装置では、泳動部２内で対流によ
る拡散が発生する。対流を防止することは困難であり、
そのため分離能が低くなる。(Problems to be Solved by the Invention) In the preparative separation apparatus shown in FIG. 3, diffusion due to convection occurs within the electrophoresis section 2. It is difficult to prevent convection,
Therefore, the resolution becomes low.
また、第３図の装置では方法論的に多検体の試料を同時
に分画することは困難である。Furthermore, with the apparatus shown in FIG. 3, it is methodologically difficult to fractionate multiple samples at the same time.
本発明は多検体の同時分画を可能とし、また分離能も向
上した分取装置を提供することを目的とするものである
。An object of the present invention is to provide a preparative fractionation device that enables simultaneous fractionation of multiple samples and has improved separation performance.
（問題点を解決するための手段）
本発明では、電気泳動用泳動管を複数本設け、各泳動管
の他端には流出液供給口、分離された試料を流出液とと
もに取り出す取出し口及び分取機構を設け、各取出し口
を通過する試料を検出器により検出する。(Means for solving the problem) In the present invention, a plurality of electrophoresis tubes are provided, and the other end of each tube is provided with an effluent supply port, a take-out port for taking out the separated sample together with the effluent, and a separation port. A sampling mechanism is provided, and a detector detects the sample passing through each extraction port.
（実施例）
第１図は一実施例を概略的に表わしたものであ、す、第
２図は１個の泳動管についての構成を示したものである
。(Example) FIG. 1 schematically shows an example, and FIG. 2 shows the configuration of one electrophoresis tube.
複数の泳動管１４が支持部材１６によって円筒状に配列
されている。泳動管１４は内径が数十ｍｍ程度のガラス
管にポリアクリルアミド・ゲル又はしよ糖などを充填し
たものである。泳動管１４の上端は電解液に浸され、−
側電極が設けられている。A plurality of migration tubes 14 are arranged in a cylindrical shape by a support member 16. The electrophoresis tube 14 is a glass tube with an inner diameter of about several tens of mm filled with polyacrylamide gel, sucrose, or the like. The upper end of the migration tube 14 is immersed in the electrolyte, -
A side electrode is provided.
泳動管１４の下端には流出液供給管１８が設けられてお
り、流出液の流れによって泳動管１４での試料の分離が
乱されないように、泳動管１４と流出液供給管１８の間
にフィルタ２０が設けられている。フィルタ２ｏの材質
としてはセラミックやポリ４フツ化エチレンなどのよう
に試料が吸着しない絶縁物が使用される。An effluent supply pipe 18 is provided at the lower end of the migration tube 14, and a filter is installed between the migration tube 14 and the effluent supply pipe 18 so that separation of the sample in the migration tube 14 is not disturbed by the flow of the effluent. 20 are provided. The filter 2o is made of an insulating material that does not attract the sample, such as ceramic or polytetrafluoroethylene.
フィルタ２０の近傍で流出液供給管１８側には試料を流
出液とともに取り出す取出し管２２が設けられている。In the vicinity of the filter 20 and on the side of the effluent supply pipe 18, there is provided a take-out pipe 22 for taking out the sample together with the effluent.
取出し管２２は三方切換えバルブ２４に接続され、三方
切換えバルブ２４の一方の出口はトラップ２６に接続さ
れ、他方の出口はドレインへ接続されている。The outlet pipe 22 is connected to a three-way switching valve 24, one outlet of which is connected to a trap 26, and the other outlet is connected to a drain.
また、フィルタ２０の近傍で流出液供給管１８側には＋
側電極が設けられている。Further, near the filter 20, on the effluent supply pipe 18 side, +
A side electrode is provided.
泳動管１４と三方切換えバルブ２４の間の取出し管２２
は透明ガラスや透明樹脂で構成され、取出し管２２には
試料が通過したことを検出する検出器が設けられている
。検出器の例としては、光吸収により検出するために光
源２８と光センサ３０とが取出し管２２を挟んで対向し
て設けられている。Output tube 22 between the migration tube 14 and the three-way switching valve 24
is made of transparent glass or transparent resin, and the extraction tube 22 is provided with a detector for detecting that the sample has passed. As an example of the detector, a light source 28 and an optical sensor 30 are provided facing each other with the extraction tube 22 in between for detection by light absorption.
３２は光センサ３０の検出信号を入力するデータ処理回
路、３４はバルブ開閉指令回路であり、バルブ開閉指令
回路３４はデータ処理回路３２からの信号によって三方
切換えバルブ２４をトラップ２６側又はドレイン側に切
り換える。32 is a data processing circuit that inputs the detection signal of the optical sensor 30; 34 is a valve opening/closing command circuit; the valve opening/closing command circuit 34 switches the three-way switching valve 24 to the trap 26 side or the drain side according to the signal from the data processing circuit 32; Switch.
泳動管１４は円筒状に複数本並べられているが、各泳動
管１４に流出液供給管１８と取出し管２２゜三方切換え
バルブ２４、光源２８及び光センサ３０が設けられてい
る。A plurality of migration tubes 14 are arranged in a cylindrical shape, and each migration tube 14 is provided with an effluent supply pipe 18, a take-out pipe 22, a three-way switching valve 24, a light source 28, and a light sensor 30.
流出液としては分離されて流出してきた試料成分が沈澱
しない電解質液が使用される。As the effluent, an electrolyte solution is used in which separated sample components do not precipitate.
次に、本実施例の動作について説明する。Next, the operation of this embodiment will be explained.
各泳動管１４の上端にそれぞれ試料を注入する。A sample is injected into the upper end of each electrophoresis tube 14, respectively.
泳動管１４の上端の一側電極と下端の＋側電極によって
泳動管１４に泳動電圧を印加し、試料を電気泳動させる
。試料は第２図にａ、ｂとして示されるように電気泳動
により分離しながら移動する。An electrophoresis voltage is applied to the electrophoresis tube 14 by one side electrode at the upper end of the electrophoresis tube 14 and a + side electrode at the lower end, and the sample is electrophoresed. The sample moves while being separated by electrophoresis as shown as a and b in FIG.
流出液供給管１８から流出液を流し、取出し管２２を経
て三方切換えバルブ２４からドレインへ排出しておく。The effluent is passed through the effluent supply pipe 18, passed through the take-out pipe 22, and discharged from the three-way switching valve 24 to the drain.
試料成分ａがフィルタ２０を通って流出液中に流入する
と、その試料成分ａが取出し管２２を通過する際光セン
サ３０によって検出される。バルブ開閉指令回路３４は
データ処理回路３２から試料を検出した信号を入力し、
その信号に基づいて三方切換えバルブ２４をトラップ２
６側に切り換え、試料ａをトラップ２６に分取する。When the sample component a flows into the effluent through the filter 20, the sample component a is detected by the optical sensor 30 as it passes through the extraction tube 22. The valve opening/closing command circuit 34 inputs the sample detection signal from the data processing circuit 32,
Based on the signal, the three-way switching valve 24 is set to trap 2.
6 side, and sample a is collected into the trap 26.
次に試料すがフィルタ２０を通って流出液に流入してき
たときは、同様にして別のトラップ２６に分取する。Next, when the sample flows into the effluent through the filter 20, it is similarly fractionated into another trap 26.
実施例では各泳動管１４について光源２８．光センサ３
０．データ処理回路３２及びバルブ開閉指令回路３４を
それぞれ１組ずつ設けているが、光源２８．光センサ３
０、データ処理回路３２及びバルブ開閉指令回路３４を
泳動管１４が配列されている円筒の軸を中心として公転
できるように支持すれば、複数の泳動管１４について１
組の光源２８．光センサ３０、データ処理回路３２及び
バルブ開閉指令回路３４ですむようになる。In the embodiment, each migration tube 14 has a light source 28 . Optical sensor 3
0. Although one set each of a data processing circuit 32 and a valve opening/closing command circuit 34 are provided, the light source 28. Optical sensor 3
0. If the data processing circuit 32 and the valve opening/closing command circuit 34 are supported so that they can revolve around the axis of the cylinder in which the migration tubes 14 are arranged, 1 for a plurality of migration tubes 14.
Set of light sources 28. The optical sensor 30, the data processing circuit 32, and the valve opening/closing command circuit 34 are now sufficient.
また、光源２８、センサ３０、データ処理回路３２及び
バルブ開閉指令回路３４を固定しておき、逆に泳動管１
４を円筒の軸を中心として公転可能に支持すれば、複数
の泳動管１４について１組の光源２８．光センサ３０、
データ処理回路３，２及びバルブ開閉指令回路３４です
むようになる。In addition, the light source 28, sensor 30, data processing circuit 32, and valve opening/closing command circuit 34 are fixed, and the migration tube 1
4 is supported so as to be able to revolve around the axis of the cylinder, one set of light sources 28 . optical sensor 30,
The data processing circuits 3 and 2 and the valve opening/closing command circuit 34 are now sufficient.
実施例では泳動管１４にポリアクリルアミド・ゲルなど
の充填物を充填しているので、対流が少なくなり、泳動
管１４の内径を太くすることができ、多量の試料を分取
することができる。しかし、泳動管１４の内径を例えば
０．５〜１ｍｍ程度に細くすれば、充填物を充填しなく
ても電解液だけで電気泳動を起させることができる。In the embodiment, since the electrophoresis tube 14 is filled with a filler such as polyacrylamide gel, convection is reduced, the inner diameter of the electrophoresis tube 14 can be increased, and a large amount of sample can be separated. However, if the inner diameter of the migration tube 14 is reduced to, for example, about 0.5 to 1 mm, electrophoresis can be caused using only the electrolytic solution without filling it with a filler.
取出し管２２を通過する試料成分を検出する手段として
は、実施例のように吸光度を測定する検出器に限らず、
例えば導電率や蛍光などを測定する検出器とすることも
できる。The means for detecting sample components passing through the extraction tube 22 is not limited to a detector that measures absorbance as in the embodiment.
For example, it can also be used as a detector that measures conductivity, fluorescence, or the like.
（発明の効果）
本発明では、電気泳動用泳動管を複数本設け、各泳動管
の他端には流出液供給口、分離された試料を流出液とと
もに取り出す取出し口及び分取機構を設け、各取出し口
を通過する試料を検出器により検出するようにしたので
、多検体試料を分離能よく同時に分取することができる
ようになる。(Effects of the Invention) In the present invention, a plurality of electrophoresis tubes are provided, and the other end of each tube is provided with an effluent supply port, an extraction port for taking out the separated sample together with the effluent, and a fractionation mechanism. Since the sample passing through each outlet is detected by the detector, multiple samples can be collected simultaneously with good resolution.
特に、多量の試料を必要とするバイオテクノロジーの分
野においては有効である。It is particularly effective in the field of biotechnology, which requires a large amount of samples.
第１図は一実施例を示す概略図、第２図は１本の泳動管
についての構成を示す概略図、第３図は従来の分取装置
を示す概略図である。
１４・・・・・・泳動管、
１８・・・・・・流出液供給管、
２２・・・・・・取出し管、
２４・・・・・・三方切換えバルブ、
２８・・・・・・光源、
３０・・・・・・光センサ、
３２・・・・・・データ処理回路、
３４・・・・・・バルブ開閉指令回路。FIG. 1 is a schematic diagram showing one embodiment, FIG. 2 is a schematic diagram showing the configuration of one electrophoresis tube, and FIG. 3 is a schematic diagram showing a conventional fractionation apparatus. 14...Migration tube, 18...Effluent supply pipe, 22...Takeout pipe, 24...Three-way switching valve, 28... Light source, 30... Optical sensor, 32... Data processing circuit, 34... Valve opening/closing command circuit.
Claims (2)
される泳動管が複数本設けられ、各泳動管の他端には流
出液供給口、分離された試料を流出液とともに取り出す
取出し口及び分取機構が設けられ、各取出し口を通過す
る試料を検出する検出器が設けられている電気泳動分取
装置。(1) A plurality of electrophoresis tubes are provided between which an electrophoresis voltage is applied between both ends and a sample is injected into one end, and at the other end of each electrophoresis tube there is an effluent supply port and an extraction port for taking out the separated sample along with the effluent. An electrophoretic separation device that is provided with a port and a separation mechanism, and is provided with a detector that detects a sample passing through each removal port.
出器は１組だけ設けられ、泳動管が配置された円筒又は
前記検出器が前記円筒の軸を中心として回転する特許請
求の範囲第１項に記載の電気泳動分取装置。(2) A plurality of the electrophoresis tubes are arranged in a cylindrical shape, only one set of the detectors is provided, and the cylinder in which the electrophoresis tubes are arranged or the detector rotates around the axis of the cylinder. The electrophoretic separation device according to scope 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62046109A JPH0789111B2 (en) | 1987-02-28 | 1987-02-28 | Electrophoretic separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62046109A JPH0789111B2 (en) | 1987-02-28 | 1987-02-28 | Electrophoretic separation device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63212853A true JPS63212853A (en) | 1988-09-05 |
| JPH0789111B2 JPH0789111B2 (en) | 1995-09-27 |
Family
ID=12737823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62046109A Expired - Fee Related JPH0789111B2 (en) | 1987-02-28 | 1987-02-28 | Electrophoretic separation device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0789111B2 (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3533933A (en) * | 1967-03-31 | 1970-10-13 | Hannig Kurt | Process and device for the isolation of fractions of a substance mixture electrophoretically separated in a carrier gel |
| JPS4727896U (en) * | 1971-04-15 | 1972-11-29 | ||
| JPS5945559U (en) * | 1982-09-20 | 1984-03-26 | アト−株式会社 | Variable migration distance type migration device |
-
1987
- 1987-02-28 JP JP62046109A patent/JPH0789111B2/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3533933A (en) * | 1967-03-31 | 1970-10-13 | Hannig Kurt | Process and device for the isolation of fractions of a substance mixture electrophoretically separated in a carrier gel |
| JPS4727896U (en) * | 1971-04-15 | 1972-11-29 | ||
| JPS5945559U (en) * | 1982-09-20 | 1984-03-26 | アト−株式会社 | Variable migration distance type migration device |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0789111B2 (en) | 1995-09-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |