JPS6319308Y2 - - Google Patents

Description

[Detailed explanation of the invention] (a) Industrial application field This invention relates to an apparatus for DNA synthesis and further RNA synthesis, and in particular relates to the water absorption mechanism of the condensation solvent.

(b) Prior art DNA synthesis consists of masking, deprotection, drying, and condensation reactions. Among these, the condensation reaction, which can be said to be the main reaction of DNA synthesis, is significantly inhibited by water. As the reaction scale becomes smaller, trace amounts of water become a problem. On the other hand, genetic engineering requires
Since the amount of DNA is very small and the nucleic acid reagents for synthesis are expensive, the reaction scale tends to become smaller and smaller. Currently, the condensation solvent is replaced with a new one every time one synthesis is completed, or Molecular Sieve is added to the liquid, but the former method is complicated to operate, and the latter method is difficult because the fine particles of Molecular Sieve are added to the solution. It can enter the flow path and damage solenoid valves, etc.

(c) Purpose This invention was developed in view of the above circumstances, and it is possible to avoid mixing water absorbing agents into the solvent and eliminate the need to replace the condensation solvent even during multiple synthesis steps.
The aim is to provide a device for synthesizing DNA, etc.

(d) Configuration In this invention, a water absorption column is incorporated into a tube extending from a condensation solvent container.
Its more detailed configuration includes a plurality of reagent containers for DNA synthesis, a condensing agent container, various reagent containers, a condensation solvent container, and a synthesis reaction container. A DNA apparatus characterized in that a water absorption column filled with a water absorption agent is incorporated into a condensation solvent extraction tube extending from a condensation solvent container in a space defined by an internal filter material.
As shown in the iso-synthesizer.

(e) Examples Examples of this invention will be described below in detail with reference to the drawings, but this invention is not limited to the following examples.

Figure 1 shows the overall configuration of this device for synthesizing DNA, etc.

Reference numeral 1 denotes an adjustment container, in which a DNA synthesis reagent container 2 and a condensing agent container 3, in which a powdered DNA synthesis reagent and a condensing agent are stored, and a condensing agent container 3 are vertically integrated. 4 is a synthesis reaction vessel in which a support is housed during the reaction. 5 is a masking reagent container; 6;
7 is a solvent container for washing and drying. 8 is a condensation solvent container, and a water absorption column 10 is installed in a condensation solvent extraction tube 9 extending from the condensation solvent container 8 to the adjustment container 1.

FIG. 1 is a schematic diagram; for example, there are actually many more adjustment containers 1, and illustrations of syringe pumps, valves, etc. are also omitted.

Briefly describing the DNA synthesis process, first, the synthesis reaction vessel 4 and the support therein are washed and dried using the solvent from the solvent vessels 6 and 7 for washing and drying. Next, the solvent is brought from the condensation solvent container 8 to one adjustment container 1, and the solvent
A DNA synthesis reagent and a condensing agent are mixed, and the resulting solution reaches the synthesis reaction vessel 4, where DNA synthesis is performed. Next, further washing, masking with a masking reagent, and washing are performed to complete the first step of DNA synthesis. Then, the flow path from the condensation solvent container 8 to the synthesis reaction container 4 is switched to yet another adjustment container 1, and the same synthesis process as described above is performed.

Below is the water absorption column 1, which is the characteristic configuration of this invention.
The configuration of the 0 portion will be explained with reference to FIGS. 2 and 3.

The water absorption column 10 is configured such that a water absorption agent 12 is provided within a column body 11. Column body 1
1 is constructed by screwing the end of a column holder 11b into a column holder 11a, and connection ports 14, 14 of tubes 13, 13 are provided at eccentric positions and central positions of the end faces of each column holder 11a, 11b. It is being The water-absorbing agent 12 is provided in the center of a cylindrical space 15 in the column body 11, and is filled and fixed in a cylindrical filter material 16 in the axial direction of the water-absorbing column 10. By positioning the water-absorbing agent 12 in this way, the water-absorbing agent 12 faces the connection port 14 on the solvent suction side, so that the solvent always passes through the water-absorbing agent 12 while reaching the connection port 14 on the discharge side. Water will definitely be taken away.

The filter material 16 is fixed in the column body 11 by pressing both ends with column holders 11a and 11b. That is, when the column holder 11b is screwed into the column holder 11a, it is fixed, and before the fixation, the water absorbing agent 12 is filled into the filter material 16.

As the water absorbing agent 12, fine particles of molecular sieve or silica gel are used, and as the filter material 16, a mouth paper, a plastic filter, a cloth filter, or a glass filter is used. Now, water absorbing agent 12
When using molecular sieve fine particles of 5 microns in size, a filter material 16 with a gap size of 1 to 2 microns is used. Such a filter material 16 does not allow the water absorbing agent 12 to pass through it, but allows the solvent to pass through it.

In the apparatus for synthesizing DNA, etc., shown in FIG. The condensation reaction takes place without being inhibited by moisture in the solvent.

In the embodiment shown in FIG. 1 above, DNA
We used an adjustment container that integrated a synthetic reagent container and a condensing agent container. The upper part of the filter 17 is used as a condensing agent container 3, and the lower part above the filter 17 is used as a DNA synthesis reagent container 2. Then, a solvent flows in from above, and a solution in which a synthetic reagent such as DNA and a condensing agent are mixed in a solvent is sent out from below.

Furthermore, in the embodiment shown in FIG. 1 above,
The condensation solvent was used to mix the DNA synthesis reagent and the condensation agent using an adjustment container, but if the DNA synthesis reagent and condensation agent are both already combined in the solvent, It is conceivable to use it as a diluting solvent for the condensation solvent, and in that case, the condensation solvent container and the water absorption column are naturally placed at appropriate positions.

That is, the condensation solvent referred to in this invention refers to a solvent that is present during condensation, and it does not matter whether its presence is positive or negative.

Furthermore, it is conceivable that the condensation solvent of the embodiment shown in FIG. 1 may be used for washing and drying purposes.

(f) Effects This idea is structured as described above, so
According to this invention, water in the condensation solvent is automatically removed by passing through the water absorption column. Therefore, using the same condensation solvent for a long time,
Even if water were to enter the condensation solvent, there would be no problem, and the same condensation solvent could be used continuously for a long time. Furthermore, since the water-absorbing agent is filled in the space defined by the filter material, the water-absorbing agent does not mix into the solvent.

[Brief explanation of the drawing]

Figure 1 is a schematic diagram of the overall configuration of an embodiment of the DNA synthesis apparatus of this invention, Figure 2 is a sectional view of an embodiment of the condensation solvent container and water absorption column of the DNA synthesis apparatus, and Figure 3 is an embodiment of the water absorption column. The cross-sectional view, FIG. 4, is a longitudinal cross-sectional view of the adjustment container. 2... DNA synthesis reagent container, 3... Condensing agent container, 4... Synthesis reaction container, 8... Condensation solvent container, 9... Drawer tube, 10... Water absorption column, 12... Water absorption agent.

Claims (1)

[Scope of utility model registration request] In a DNA synthesis apparatus that is equipped with a plurality of DNA synthesis reagent containers, a condensation agent container, various reagent containers, a condensation solvent container, and a synthesis reaction container, and in which DNA or the like is synthesized in the synthesis reaction container, the condensation solvent 1. An apparatus for synthesizing DNA, etc., characterized in that a water absorption column filled with a water absorption agent is incorporated into a condensation solvent draw-out tube extending from a container, in a space defined by an internal filter material.