JPS6315817Y2 - - Google Patents

Description

[Detailed Description of the Invention] The present invention relates to an improvement in a container for filling a so-called support gel used in an immunological testing method called a one-way immunodiffusion method.

One-way immunodiffusion method is a simple method to measure the amount of antigen or antibody contained in a test sample, and it forms a gel containing a constant concentration of the antibody or antigen corresponding to the antigen or antibody in a thin plate shape. Then, a small diameter hole is made in the gel, a test sample is injected into the hole, and the test sample is injected into the hole and incubated under appropriate conditions for a certain period of time to cause an antigen-antibody reaction. The diameter of the (sedimentation ring) is measured and the amount of antigen or antibody in the test sample is determined from this. That is, when the concentration of antibody or antigen in the gel is constant and the thickness of the gel is constant, the amount of antigen or antibody contained in the test sample is proportional to the area surrounded by the sedimentation ring. That's what I do. In this method, since the reaction part is thin and flat, the amount of antigen or antibody in the test sample is very sensitively reflected in the ring diameter, and is highly quantitative. Normally, it is necessary to read the size of the settling ring to the nearest 0.1 mm, so a magnifying glass with a scale is used for this purpose.

The gel flat plate used for the above testing method is formed by filling a container with a flat bottom with gel to a certain thickness. This container should be sufficiently large compared to the size of the sedimentation ring, and a large number of test sample injection holes should be placed at sufficient intervals so that the rings do not overlap on one side of the gel plate. Usually, a test sample is injected into each of the injection holes and incubated at the same time. At this time, record the number of the test sample injected into each injection hole and make it correspond to the ring diameter measured after incubation. However, since the ring diameter is measured using a magnifying glass with a scale, the field of view during measurement is extremely narrow, so the measurer cannot immediately confirm which injection hole the ring diameter under measurement relates to, and sometimes It is possible to make a mistake in the correspondence between the test sample number and the measurement ring diameter.

In view of the above-mentioned current situation, the present invention is designed to accurately and easily correspond to the test sample number and the measuring ring diameter even when testing is performed with a large number of test sample injection holes. The purpose of this is to provide a container for filling an improved support gel, and due to its structure, a code with a shape smaller than the diameter of the test sample injection hole drilled in the gel in the above test is placed on the bottom. It is characterized in that a plurality of them are provided at appropriate intervals.

Hereinafter, the present invention will be further explained with reference to the drawings.

FIG. 1 is a plan view of the container of the present invention, and FIG. 2 is a sectional view thereof. The container of this invention has a flat bottom part 1.
It is a dish-shaped container having a shape and size, etc., and is not particularly limited. The material of the container is acrylic.
Transparent or translucent synthetic resins such as polyethylene, polypropylene, polystyrene, polycarbonate, etc. are preferred. A plurality of numerals 2 are attached to the bottom 1 of the container at appropriate intervals. Although the reference numeral 2 is a number in FIG. 1, alphabets, katakana, etc., combinations thereof, and other appropriate codes may be used. The size of each code 2 is smaller than the diameter of the test sample injection hole (usually 2.5 to 3.0 mm) drilled in the gel during testing, for example, 1.5 to 3.0 mm.
Set to 2.0mm. Further, the intervals between the symbols 2 are set to such an extent that the sedimentation rings formed on the gel do not overlap during the inspection, for example, 10 mm or more. The method of applying the code 2 to the container bottom 1 is not particularly limited, and the second
It may be formed in a concavo-convex shape on the inner or outer surface of the container as shown in 2' or 2'' in the figure, or it may be formed by printing or pasting a sticker on the inner or outer surface of the container.Of course, 2' in FIG. It may be formed inside the container bottom 1 as shown in FIG.

When using the container of the present invention as described above, as in the past, apply a thin layer of gel A on the bottom 1 of the container, for example.
Fill the gel into a flat plate with a uniform thickness of 1.0 to 2.0 mm, make a test sample injection hole a at the position corresponding to code 2 in this gel, and insert the test sample into the injection hole a according to the usual method. Inject B. Here, the correspondence between the code 2 and the test sample number injected into the injection hole a of that code is recorded. Next, when incubation is performed in the same manner as before, the circular portions of the flat gel A having each injection hole a at the center react, and a sedimentation ring r appears. Therefore, it is possible to read the diameter of each ring r using a magnifying glass with a scale, and at the same time read the code 2 at the position of the injection hole a in the center, ensuring that the measured ring diameter corresponds to the code 2 to which the ring belongs. can be recorded.

As described above, according to the container of the present invention, even when testing a large number of test samples at the same time in the unified immunodiffusion method, it is possible to match the test sample and the measuring ring diameter extremely easily and accurately. Never make a mistake.

Incidentally, the present invention also includes a container having a plurality of small gel filling portions 3 as shown in cross-section in FIG.

[Brief explanation of the drawing]

FIG. 1 is a plan view of the container of the present invention, and FIGS. 2 and 3 are longitudinal sectional views of the container of the present invention. 1... Container, 2, 2', 2'', 2... Code, 3
... Gel filling part, A ... Support gel, B ... Test sample, a ... Test sample injection hole, r ... Sedimentation ring.

Claims (1)

[Scope of utility model registration request] In a container for filling the support gel used in the one-way immunodiffusion method, multiple codes with a shape smaller than the diameter of the test sample injection hole drilled into the gel in the test are given at appropriate intervals on the bottom. A container for filling a support gel, characterized in that: