JPS63157975A - Production of cell of lactic bacteria - Google Patents
Production of cell of lactic bacteriaInfo
- Publication number
- JPS63157975A JPS63157975A JP30115786A JP30115786A JPS63157975A JP S63157975 A JPS63157975 A JP S63157975A JP 30115786 A JP30115786 A JP 30115786A JP 30115786 A JP30115786 A JP 30115786A JP S63157975 A JPS63157975 A JP S63157975A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- lactic acid
- acid bacteria
- culture
- waste
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 51
- 238000004519 manufacturing process Methods 0.000 title claims description 5
- 239000002699 waste material Substances 0.000 claims abstract description 24
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 230000001580 bacterial effect Effects 0.000 claims abstract description 8
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 7
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 84
- 239000004310 lactic acid Substances 0.000 claims description 42
- 235000014655 lactic acid Nutrition 0.000 claims description 42
- 238000012258 culturing Methods 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 7
- 239000008103 glucose Substances 0.000 abstract description 7
- 238000004065 wastewater treatment Methods 0.000 abstract description 4
- 241000500332 Tetragenococcus halophilus Species 0.000 abstract description 3
- 210000004748 cultured cell Anatomy 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 27
- 239000001963 growth medium Substances 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000012141 concentrate Substances 0.000 description 6
- 235000013555 soy sauce Nutrition 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000012136 culture method Methods 0.000 description 3
- GNBVPFITFYNRCN-UHFFFAOYSA-M sodium thioglycolate Chemical compound [Na+].[O-]C(=O)CS GNBVPFITFYNRCN-UHFFFAOYSA-M 0.000 description 3
- 229940046307 sodium thioglycolate Drugs 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- 241000192132 Leuconostoc Species 0.000 description 2
- 241000192001 Pediococcus Species 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000194031 Enterococcus faecium Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 235000013958 Lactobacillus casei Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000007221 ypg medium Substances 0.000 description 1
Abstract
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、乳酸菌４体の製造法に関する。[Detailed description of the invention] [Industrial application field] The present invention relates to a method for producing four lactic acid bacteria.
従来、醤油の工業的生産に際しては、醤油諸株に、種菌
として有用醤油乳酸菌、酵母を添加して製品醤油の品質
向上を計っている。しかし、各工場で乳酸菌を培養する
ことは、培養設備上極めて困難であるため、集中的に乳
酸菌の培養を行ない、得られた培養物を濃縮して運搬効
率を高めたのち、工場に配布されているのが実情である
。Conventionally, in the industrial production of soy sauce, useful soy sauce lactic acid bacteria and yeast are added as seed bacteria to soy sauce strains in order to improve the quality of the product soy sauce. However, it is extremely difficult to cultivate lactic acid bacteria at each factory due to the cultivation equipment, so we cultivate lactic acid bacteria intensively and concentrate the resulting culture to increase transportation efficiency before distributing it to factories. The reality is that
しかしながら、その際、必然的に多量の廃培地が生成さ
れるのであるが、そのままでは、乳酸菌の培地として再
利用することは不可能であった。However, in this case, a large amount of waste medium is inevitably produced, but it has been impossible to reuse it as a medium for lactic acid bacteria.
そして、廃培地は、極めてＢ、Ｏ，Ｄ、の高い有機性の
ものである。このため、そのままでは廃棄できす、廃水
処理が設されて廃棄されるのであるが、その廃水の処理
設備の建造及び運転のためには著しく資金が必要となる
等の欠点があった。The waste culture medium is extremely organic with high B, O, and D contents. For this reason, it cannot be disposed of as it is, or it can be disposed of by installing wastewater treatment facilities, but it has drawbacks such as the significant amount of funds required to construct and operate the wastewater treatment equipment.
そこで、本発明者等は、何の用途もなく廃棄されている
乳酸菌廃培地の用途について種々検討した結果、乳酸菌
を液体培地に培養し、培養物より菌体な除去あるいは濃
縮して生成される廃培地に、乳酸菌の資化可能な炭素源
を添加し、かつ、乳酸菌の生育可能なｐＨに調整さえす
れば、乳酸菌の培地として再び利用することができるこ
と等を見出し、本発明は、その知見に基づいて完成され
たものである。Therefore, as a result of various studies on the uses of lactic acid bacteria waste culture medium that is discarded without any use, the present inventors cultivated lactic acid bacteria in a liquid medium, and removed or concentrated the bacterial cells from the culture. It has been discovered that a waste medium can be reused as a medium for lactic acid bacteria by adding a carbon source that can be assimilated by lactic acid bacteria and adjusting the pH to a level at which lactic acid bacteria can grow, and the present invention is based on that knowledge. It was completed based on.
すなわち本発明は、乳酸菌を液体培地に培養し、培養物
より菌体を除去あるいは濃縮して生成される廃培地に、
乳酸菌の資化可能な炭素源を添加し、かつ、乳酸菌の生
育可能なｐＨに調整したものを培地として再利用し、乳
酸菌を培養することを特徴とする乳酸菌４体の製造法で
ある。That is, the present invention cultivates lactic acid bacteria in a liquid medium, and removes or concentrates bacterial cells from the culture to produce a waste medium,
This is a method for producing four lactic acid bacteria, which is characterized by adding a carbon source that can be assimilated by lactic acid bacteria and adjusting the pH to a level that allows lactic acid bacteria to grow, and reusing it as a medium to culture lactic acid bacteria.
以下、本発明について詳細に説明する。The present invention will be explained in detail below.
先ず、本発明において用いられる乳酸菌としては、如何
なるものでもよく、例えば、ペディオコッカス属、スト
レプトコツカス属、ラクトバチルス属、ロイコノストッ
ク属等に属する菌株が挙げられ、その具体例としては、
ペディオコッカス・ハロフィルスＮｏ、１５４７ （Ｆ
ＥＲＭ ＢＰ−８１９） 、ストレプトコッカス・ファ
エシウム（ＡＴＣＣ８０４３）、ラクトバチルス・カゼ
イ （ＡＴＣＣ７４６９） 、ロイコノストック・メツ
センチロイデス （ＩＦＯ３４２６） 等であり、それ
らのうち、耐塩性乳酸菌であるペディオコッカス・ハロ
フィルスＮｏ、１５４７ （ＦＥＲＭ乳酸菌の液体培
地としては、例えば、酵母エキス０．３％ （Ｗ／Ｖ）
、ポリペプトン１％ （Ｗ／Ｖ）、グルコース１％ （
Ｗ／Ｖ）、リン酸二カリウム０６５％（Ｗ／Ｖ）及びチ
オグリコール酸ナトリウム０．１％（Ｗ／Ｖ）からなる
ＹＰＧ培地、肉エキス１％（Ｗ／Ｖ）、ポリペプトン１
％（Ｗ／Ｖ） 、酵母エキス１％（Ｗ／Ｖ）、グルコー
ス１％（Ｗ／Ｖ）、及びチオグリコール酸ナトリウム０
．１％（Ｗ／Ｖ）からなるＭＶＰ培地等が挙げられる。First, any lactic acid bacteria may be used in the present invention, and examples thereof include strains belonging to the genus Pediococcus, genus Streptococcus, genus Lactobacillus, genus Leuconostoc, etc. Specific examples thereof include:
Pediococcus halophilus No. 1547 (F
ERM BP-819), Streptococcus faecium (ATCC8043), Lactobacillus casei (ATCC7469), Leuconostoc metsucentiloides (IFO3426), etc. Among them, Pediococcus halophilus, which is a salt-tolerant lactic acid bacterium, No. 1547 (As a liquid medium for FERM lactic acid bacteria, for example, yeast extract 0.3% (W/V)
, polypeptone 1% (W/V), glucose 1% (
W/V), YPG medium consisting of dipotassium phosphate 065% (W/V) and sodium thioglycolate 0.1% (W/V), meat extract 1% (W/V), polypeptone 1
% (W/V), yeast extract 1% (W/V), glucose 1% (W/V), and sodium thioglycolate 0
．． Examples include MVP medium containing 1% (W/V).
なお、上記乳酸菌のうちペディオコッカス・・・ロフイ
ルス歯、１５４７ （ＦＥＲＭ ＢＰ−８１９）は、
耐塩性乳酸菌であるため、該菌の培養に際しては、ＹＰ
Ｇ培地、ＭＶＰ培地に、更に、食塩を例えば、５〜１５
％（Ｗ／Ｖ）程度添加した培地が用いられる。In addition, among the lactic acid bacteria mentioned above, Pediococcus...Lofilus tooth, 1547 (FERM BP-819) is
Since it is a salt-tolerant lactic acid bacterium, when culturing this bacterium, YP
G medium and MVP medium are further added with, for example, 5 to 15 ml of salt.
% (W/V) is used.
そして、前記乳酸菌を、このような液体培地に、例えば
温度２５〜３５°Ｃ１好ましくは３０°Ｃ前後で、初発
ｐＨ４〜９、好ましくは、７前後で、１２〜１２０時間
程度培養し、培養物を得る。Then, the lactic acid bacteria are cultured in such a liquid medium at a temperature of 25 to 35°C, preferably around 30°C, and an initial pH of 4 to 9, preferably around 7, for about 12 to 120 hours. get.
また、培養方法としては、例えば、静置培養法が好適で
ある。Moreover, as a culture method, for example, a static culture method is suitable.
次いで、このようにして得た培養物より、乳酸菌４体を
除去あるいは濃縮して菌体もしくはその濃縮物及び廃培
地を得る。Next, from the culture thus obtained, four lactic acid bacteria are removed or concentrated to obtain bacterial cells or a concentrate thereof and a waste medium.
乳酸菌４体を除去あるいは濃縮する方法としては、如何
なる方法でもよく、例えば、ペリコンカセット、プロス
タツク（何れもミリボア社製、限外濾過機）を用いて乳
酸菌４体を除去あるいは濃縮する方法、また、遠心分離
機を用いて、５．００Ｏｒ、ｐ、ｍ、以上、好ましくは
８．００Ｏｒ、ｐ、ｍ、前後で１０分間以上、好ましく
はｌＯ〜２０分間程度遠心分離処理する方法等が挙げら
れる。Any method may be used to remove or concentrate the four lactic acid bacteria; for example, a method of removing or concentrating the four lactic acid bacteria using a Pellicon cassette or Prostack (both manufactured by Millibore, ultrafilter); Examples include a method of centrifuging using a centrifuge at 5.00 Or, p, m or more, preferably around 8.00 Or, p, m, for 10 minutes or more, preferably about 10 to 20 minutes.
そして、本願発明においては、乳酸菌４体の製造に際し
生成される廃培地に、乳酸菌の資化可能な炭素源を添加
し、かつ、廃培地のｐＨを、乳酸菌の生育可能なｐＨ１
例えば、４〜９、好ましくは７程度に調整したものを培
地として再利用し、乳酸菌を上述したと同様の培養方法
により培養して培養物を得、これから上述の方法により
菌体な除去あるいは濃縮して乳酸菌４体もしくはその濃
縮物を得るのである。In the present invention, a carbon source that can be assimilated by lactic acid bacteria is added to the waste medium produced during the production of four lactic acid bacteria, and the pH of the waste medium is adjusted to 1, which is a pH at which lactic acid bacteria can grow.
For example, reuse the culture medium adjusted to 4 to 9, preferably about 7, and culture lactic acid bacteria using the same culture method as described above to obtain a culture, and then remove or concentrate the bacterial cells using the method described above. Then, four lactic acid bacteria or their concentrates are obtained.
乳酸菌の資化可能な炭素源としては、例えば、グルコー
ス、フラクトース、アラビノース、ラクトース、クエン
酸等が挙げられ、それらのうちグルコースは、好適であ
り、該炭素源は、単独にあるいは組み合わせて用いるこ
とができ、また、廃培地への添加濃度は、例えば、０．
５〜１．５％（Ｗ／Ｖ）、好ましくは、１％（Ｗ／Ｖ）
程度である。Examples of carbon sources that can be assimilated by lactic acid bacteria include glucose, fructose, arabinose, lactose, citric acid, etc. Among them, glucose is preferred, and the carbon sources can be used alone or in combination. can be added, and the concentration added to the waste medium is, for example, 0.
5-1.5% (W/V), preferably 1% (W/V)
That's about it.
また、廃培地のｐＨ調整剤としては、アルカリであれば
如何なるものでもよく、例えば、水酸化ナトリウム、水
酸化カリウム、炭酸水素ナトリウム等のものが挙げられ
る。Further, as the pH adjusting agent for the waste culture medium, any alkaline agent may be used, and examples thereof include sodium hydroxide, potassium hydroxide, sodium hydrogen carbonate, and the like.
なお、再生した廃培地を再利用して乳酸菌を培養する場
合、最初に用いた乳酸菌と同一の菌株を用いる場合には
、再生した廃培地の殺菌処理は不必要であるが、異なる
乳酸菌４株を用いる場合には、常法により再生した廃培
地の殺菌処理を行なうことが必要である。Note that when reusing the recycled waste medium to culture lactic acid bacteria, sterilization of the recycled waste medium is not necessary if the same strain as the lactic acid bacteria originally used is used, but four different strains of lactic acid bacteria are used. When using a medium, it is necessary to sterilize the recycled waste culture medium using a conventional method.
そして、廃培地は、上述した廃培地の再生処理と培養を
繰り返すことにより、乳酸菌の生育が困難となるまで培
地として再利用することが可能であるが、通常は、数回
程度再利用することができる。The waste medium can be reused as a medium until it becomes difficult for lactic acid bacteria to grow by repeating the waste medium regeneration treatment and culturing described above, but normally it is only reused several times. I can do it.
以上の如く、本発明によれば、従来例の用途もなく廃棄
されていた乳酸菌の廃培地を、簡易な操作により、培地
として再利用することができ、また、廃培地として生成
される量も従来法に比して極めて減少し、その廃水処理
コストも著しく低減されるため、本発明は、産業上極め
て有利である。As described above, according to the present invention, the waste culture medium of lactic acid bacteria, which was previously discarded without any use, can be reused as a culture medium through simple operations, and the amount produced as waste culture medium can also be reduced. The present invention is industrially extremely advantageous because the wastewater treatment cost is significantly reduced compared to conventional methods.
以下、実施例を挙げて本発明をさらに具体的に説明する
。Hereinafter, the present invention will be explained in more detail with reference to Examples.
実施例
肉エキス１％ （Ｗ／Ｖ）、ポリペプトン１％（Ｗ／Ｖ
）、酵母エキス１％（Ｗ／Ｖ）、グルコース１％ （Ｗ
／Ｖ） 、チオグリコール酸ナトリウム０．１％（Ｗ／
Ｖ）及び食塩１５％（Ｗ／ｖ）ノ組成からなるＭＶＰ培
地（ｐＨ７，２） ５ ｔｔを、常法により殺菌しタモ
のに、ヘティオコツカス・ハロフィルス翫１５４７
（ＦＥＲＭ ＢＰ−８１９）を接種し、温度３０°Ｃで
７２時間静置培養し、培養液５７Ｉを得た。Example meat extract 1% (W/V), polypeptone 1% (W/V
), yeast extract 1% (W/V), glucose 1% (W
/V), sodium thioglycolate 0.1% (W/
MVP medium (pH 7,2) 5 tt consisting of V) and 15% (W/v) salt was sterilized by a conventional method, and Hetiococcus halophilus 1547
(FERM BP-819) was inoculated and statically cultured at a temperature of 30°C for 72 hours to obtain culture solution 57I.
次いで、該培養液を、高速遠心分離機（日立製作所・株
・製、５ＣＲ２ＯＢ型）を用いて、温度４°Ｃに保持し
つつ８．００Ｏｒ、ｐ、ｍ、で１０分間処理し、湿潤菌
体１０ ｇ及び上清的５４を廃培地として得た。Next, the culture solution was treated at 8.00 Or, p, m for 10 minutes using a high-speed centrifuge (manufactured by Hitachi, Ltd., model 5CR2OB) while maintaining the temperature at 4°C, to remove the wet bacteria. 10 g of the body and 54 g of the supernatant were obtained as waste medium.
なお、得られた湿潤菌体は、乳酸発酵用種菌として醤油
諸株に添加した。The obtained wet bacterial cells were added to various soy sauce strains as starter bacteria for lactic acid fermentation.
次いで、このようにして得られた廃培地５７！にグルコ
ースを１％ （Ｗ／Ｖ）となる如く添加し、更に、廃培
地のｐＨを３Ｎの水酸化ナトリウム水溶液を用いて７．
０に調整したものを培地として用い、これに、上記乳酸
菌を接種して上述と同一の条件下に培養を行ない、更に
、上述の分離処理を行なう操作を２回繰り返すことによ
り、２回目に、９ｇ１３回目に８Ｉの湿潤菌体を得た。Next, the waste culture medium obtained in this way 57! Glucose was added to the solution at a concentration of 1% (W/V), and the pH of the waste medium was adjusted to 7.5% using a 3N aqueous sodium hydroxide solution.
Using the medium adjusted to 0 as a medium, inoculating it with the lactic acid bacteria and culturing it under the same conditions as above, and repeating the above separation process twice, the second time, At the 13th time of 9g, wet bacterial cells of 8I were obtained.
Claims (1)
去あるいは濃縮して生成される廃培地に、乳酸菌の資化
可能な炭素源を添加し、かつ、乳酸菌の生育可能なｐＨ
に調整したものを培地として再利用し、乳酸菌を培養す
ることを特徴とする乳酸菌々体の製造法。(1) A carbon source that can be assimilated by lactic acid bacteria is added to the waste medium produced by culturing lactic acid bacteria in a liquid medium and removing or concentrating bacterial cells from the culture, and the pH is adjusted to a level that allows lactic acid bacteria to grow.
1. A method for producing lactic acid bacteria cells, which comprises reusing the adjusted medium as a medium and culturing lactic acid bacteria.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30115786A JPS63157975A (en) | 1986-12-19 | 1986-12-19 | Production of cell of lactic bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30115786A JPS63157975A (en) | 1986-12-19 | 1986-12-19 | Production of cell of lactic bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS63157975A true JPS63157975A (en) | 1988-06-30 |
Family
ID=17893467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP30115786A Pending JPS63157975A (en) | 1986-12-19 | 1986-12-19 | Production of cell of lactic bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63157975A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5847485A (en) * | 1981-08-18 | 1983-03-19 | デ−・エル・エム・ドクトル・ミユラ−・アクチエンゲゼルシャフト | Method and apparatus for culturing microorganism |
-
1986
- 1986-12-19 JP JP30115786A patent/JPS63157975A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5847485A (en) * | 1981-08-18 | 1983-03-19 | デ−・エル・エム・ドクトル・ミユラ−・アクチエンゲゼルシャフト | Method and apparatus for culturing microorganism |
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