JPS6315161A - Blood sampling tube for blood test - Google Patents
Blood sampling tube for blood testInfo
- Publication number
- JPS6315161A JPS6315161A JP15914486A JP15914486A JPS6315161A JP S6315161 A JPS6315161 A JP S6315161A JP 15914486 A JP15914486 A JP 15914486A JP 15914486 A JP15914486 A JP 15914486A JP S6315161 A JPS6315161 A JP S6315161A
- Authority
- JP
- Japan
- Prior art keywords
- blood
- acid
- test
- edta
- pref
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000009534 blood test Methods 0.000 title claims description 15
- 238000010241 blood sampling Methods 0.000 title abstract description 4
- 210000004369 blood Anatomy 0.000 claims abstract description 62
- 239000008280 blood Substances 0.000 claims abstract description 62
- 239000003146 anticoagulant agent Substances 0.000 claims abstract description 10
- 229940127219 anticoagulant drug Drugs 0.000 claims description 8
- 229940079593 drug Drugs 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
- 150000007522 mineralic acids Chemical class 0.000 claims description 5
- 150000007524 organic acids Chemical class 0.000 claims description 5
- 230000002378 acidificating effect Effects 0.000 claims description 4
- 230000002429 anti-coagulating effect Effects 0.000 claims description 2
- 238000010979 pH adjustment Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 11
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract description 9
- 239000002253 acid Substances 0.000 abstract description 7
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 abstract description 6
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 abstract description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 abstract description 4
- QLBHNVFOQLIYTH-UHFFFAOYSA-L dipotassium;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [K+].[K+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O QLBHNVFOQLIYTH-UHFFFAOYSA-L 0.000 abstract description 4
- UEZVMMHDMIWARA-UHFFFAOYSA-N Metaphosphoric acid Chemical compound OP(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-N 0.000 abstract description 2
- 229960005070 ascorbic acid Drugs 0.000 abstract description 2
- 235000010323 ascorbic acid Nutrition 0.000 abstract description 2
- 239000011668 ascorbic acid Substances 0.000 abstract description 2
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 abstract description 2
- 239000011976 maleic acid Substances 0.000 abstract description 2
- 239000001509 sodium citrate Substances 0.000 abstract description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 abstract description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 abstract description 2
- 229940127090 anticoagulant agent Drugs 0.000 abstract 2
- 230000004520 agglutination Effects 0.000 abstract 1
- 230000001455 anti-clotting effect Effects 0.000 abstract 1
- 239000013043 chemical agent Substances 0.000 abstract 1
- JZBRFIUYUGTUGG-UHFFFAOYSA-J tetrapotassium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [K+].[K+].[K+].[K+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O JZBRFIUYUGTUGG-UHFFFAOYSA-J 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 15
- 238000004820 blood count Methods 0.000 description 9
- 210000001772 blood platelet Anatomy 0.000 description 9
- 210000003743 erythrocyte Anatomy 0.000 description 8
- 210000000265 leukocyte Anatomy 0.000 description 7
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical class OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 206010018910 Haemolysis Diseases 0.000 description 2
- 230000023555 blood coagulation Effects 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- -1 TA-3K are added Chemical class 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 206010043554 thrombocytopenia Diseases 0.000 description 1
Abstract
Description
【発明の詳細な説明】
[発明の背景]
(技術分野)
この発明は血液中の赤血球、白血球および血小板の数を
正確に測定できるようにした血液検査用採血管に関する
。DETAILED DESCRIPTION OF THE INVENTION [Background of the Invention] (Technical Field) The present invention relates to a blood test blood collection tube that allows accurate measurement of the number of red blood cells, white blood cells, and platelets in blood.
(先行技術および問題点)
血液検査において血球数検査がおこなわれるが、血球数
検査は血液中に含まれる赤血球、白血球および血小板の
数を測定するためのものであり、最も頻繁に実施されて
いる検査の一つである。この検査を実施するに際し、適
当な血液抗凝固剤を用いて血液の凝固や溶血を防ぐ必要
がある。さらに、この検査は血液像検査と合わせておこ
なわれることが多いので、使用する血液抗凝固剤は血液
像を変化させないものであることが望ましい。(Prior Art and Problems) A blood cell count test is performed in a blood test, and the blood cell count test is the most frequently performed test that measures the number of red blood cells, white blood cells, and platelets contained in the blood. This is one of the inspections. When performing this test, it is necessary to use an appropriate blood anticoagulant to prevent blood coagulation and hemolysis. Furthermore, since this test is often performed in conjunction with a blood image test, it is desirable that the blood anticoagulant used does not change the blood image.
そのため、従来の血球数検査をおこなうための採血管に
はEDTA−2Na 、 EDTA −2K 、 ED
TA−3K等のEDTA塩が添加されており、赤血球数
、白血球数および血液像検査においては良好な成績が得
られている。Therefore, blood collection tubes for conventional blood cell count tests include EDTA-2Na, EDTA-2K, and EDTA-2Na.
EDTA salts such as TA-3K are added, and good results have been obtained in red blood cell counts, white blood cell counts, and blood image tests.
しかし、血小板数検査に関しては必ずしも正確な測定が
なされていないことが見い出された。す々わち、従来の
血球数検査のための採血管には上述の如きEDTA塩を
単に血液の凝固を防ぐことのみを主眼として添加してい
るにすぎず、そのため血小板の凝集を充分に抑えること
ができ危かったためである。したがって、血小板数が経
時的に減少してしまい、検査値に誤差が生じていた。However, it has been found that platelet count tests are not always accurate. In other words, the above-mentioned EDTA salt is simply added to blood collection tubes for blood cell count tests with the main purpose of preventing blood coagulation, and therefore platelet aggregation is sufficiently suppressed. This was because it was dangerous. Therefore, the platelet count decreased over time, causing errors in test values.
[発明の目的]
この発明安置前は赤血球数、白血球数および血液像等の
他の検査項目に何んら影響を及ぼさずに、経時的な血小
板凝集を抑え、これにより血小板数の正確な測定を可能
にする血液検査用採血管を提供することを目的とする。[Purpose of the invention] This invention suppresses platelet aggregation over time without affecting other test items such as red blood cell count, white blood cell count, and blood image, and thereby enables accurate measurement of platelet count. The purpose of the present invention is to provide blood test blood collection tubes that enable blood testing.
すなわち、この発明は有底管体と、この管体内に封入さ
れた薬品とを具備してなる血液検査用採血管において、
該薬品が血液抗凝固作用と採血した血液の戸を5.0な
いし7.0、好ましくは6.0ないし7.Olよシ好ま
しくは6.5ないし7.0にする作用を有することを特
徴とする血液検査用採血管を提供するものである。That is, the present invention provides a blood test blood collection tube comprising a bottomed tube and a drug sealed in the tube.
The drug has a blood anticoagulant effect and a blood sampling rate of 5.0 to 7.0, preferably 6.0 to 7.0. The purpose of the present invention is to provide a blood sampling tube for blood testing, which is characterized by having the effect of adjusting the OL to preferably 6.5 to 7.0.
さらに、この発明は上記範囲のpHの調整を酸性の血液
抗凝固剤の使用によっておこなう具体的態様、および有
機酸又は無機酸の添加によっておこなう具体的態様を提
供するものである。Furthermore, the present invention provides a specific embodiment in which the pH within the above range is adjusted by using an acidic blood anticoagulant, and a specific embodiment in which the adjustment is carried out by the addition of an organic or inorganic acid.
[発明の詳細な説明] 以下、この発明を図示の実施例を参照して説明する。[Detailed description of the invention] The present invention will be described below with reference to illustrated embodiments.
第1図はこの発明の一実施例に係わる血液検査用採血管
の断面を示すものであって、有底管体I(5ゴ管)の底
部に血液抗凝固剤および有機酸(又は無機酸)からなる
試薬2が粉末状で封入されている。FIG. 1 shows a cross section of a blood test blood collection tube according to an embodiment of the present invention, in which a blood anticoagulant and an organic acid (or inorganic acid ) is enclosed in powder form.
この採血管内に血液を所定量採血し、血液を試薬2と混
和した場合、血液は試薬中の酸の割合により−が自然に
5.0〜7.0、好ましくは6.0〜7.0、より好ま
しくは6.5〜7.0となるようになっている。血液の
−がこの範囲に調整されることKより、血小板の凝集を
実質的に防止することが可能となる。血液のpHを5以
下とした場合は溶血が起シ、赤血球数の正確な測定が困
難となり、逆に−が7を超えたときは血小板の凝集が起
り正確な血小板数の測定が不可能となる
・この場合の使用可能々血液抗凝固剤の例としては従来
使用されているものと同様のもの、たとえば、クエン酸
ナトリウム、EDTA −2Na、EDTA−2K 。When a predetermined amount of blood is collected into this blood collection tube and the blood is mixed with reagent 2, the blood will naturally have a - value of 5.0 to 7.0, preferably 6.0 to 7.0, depending on the proportion of acid in the reagent. , more preferably 6.5 to 7.0. By adjusting the - of blood to within this range, platelet aggregation can be substantially prevented. If the pH of the blood is less than 5, hemolysis occurs, making it difficult to accurately measure the number of red blood cells.On the other hand, if the pH of the blood exceeds 7, platelet aggregation occurs, making it impossible to accurately measure the number of platelets. Examples of blood anticoagulants that can be used in this case are the same as those conventionally used, such as sodium citrate, EDTA-2Na, and EDTA-2K.
EDTA−3に、その他のEDTA塩を用いることがで
きる。Other EDTA salts can be used with EDTA-3.
有機酸としてはクエン酸、マロン酸、マレイン酸、アス
コルビン酸等を使用することができる。As the organic acid, citric acid, malonic acid, maleic acid, ascorbic acid, etc. can be used.
無機酸としては硫酸、メタリン酸等を使用することがで
きる。As the inorganic acid, sulfuric acid, metaphosphoric acid, etc. can be used.
なお、第1図の例においては試薬2中に有機酸、又は無
機酸を用いる態様について説明したが、これらの酸を全
く用いずに血液抗凝固剤として酸性のものを選び、採取
した血液のpHを上記範囲内に調整することもできる。In addition, in the example shown in Figure 1, an embodiment in which an organic acid or an inorganic acid is used in the reagent 2 was explained. The pH can also be adjusted within the above range.
このような酸性血液抗凝固剤の例としてはEDTA −
2Na 、 EDTA −2Kがある。Examples of such acidic blood anticoagulants include EDTA-
There are 2Na and EDTA-2K.
試薬の形態については特に制限はなく、第1図に示した
粉体のほか、第2図にて参照符号3で示す如き顆粒状の
もの、あるいは第3図に参照符号4で示す如き液状のも
のであってもよい。なお、第2図および第3図に示す血
球数検査用採血管は試薬の形態についてのみ第1図の例
と異なるものであり、その他九ついては第1図の例と同
一である。There are no particular restrictions on the form of the reagent, and in addition to the powder shown in Figure 1, granular forms as shown by reference numeral 3 in Fig. 2, or liquid forms as shown by reference numeral 4 in Fig. 3 are also available. It may be something. The blood cell count blood collection tube shown in FIGS. 2 and 3 differs from the example shown in FIG. 1 only in the form of the reagent, and the other nine points are the same as the example shown in FIG.
なお、試薬2.3.4の封入位置は上記例の如く管体1
底部に限らず任意の場所に配置させてもよい。Note that the reagent 2.3.4 is sealed in tube body 1 as in the above example.
It may be placed not only at the bottom but also at any desired location.
このような血液検査用採血管は通常、開ロ部ゴム役等で
封止し、真空採血管の形態にして用いられる。しかし、
もちろんそれ以外の形態で使用してもよい。Such blood test blood collection tubes are usually sealed with a rubber closure or the like and used in the form of a vacuum blood collection tube. but,
Of course, it may be used in other forms.
実施例
内径10mの円筒状採血管に血液を採取し、直ちに血液
1m当りEDTA −2Na 5 q、クエン酸5■を
添加し混和した。これら薬剤添加前の血液の−は7.4
であり、添加後は5,2となった。ついで赤血球数、白
血球数および血小板数の検査を、混和直後、混和2時間
後、混和6時間後についておこなった。その結果を下記
浅に示す。この表から明らかな如く、これら血球数の経
時的変化は見られず、経時的な血小板数の減少、すなわ
ち凝集は認められなかっ之。Example Blood was collected into a cylindrical blood collection tube with an inner diameter of 10 m, and immediately 5 q of EDTA-2Na and 5 q of citric acid were added and mixed per 1 m of blood. -7.4 of blood before addition of these drugs
After addition, it became 5.2. Next, the number of red blood cells, white blood cells, and platelets were tested immediately after mixing, 2 hours after mixing, and 6 hours after mixing. The results are shown below. As is clear from this table, no change in the number of blood cells over time was observed, and no decrease in the number of platelets over time, that is, no aggregation was observed.
また、血液像検査についても同時におこなったが、何ん
らの影響も認められなかった。A blood image test was also performed at the same time, but no effects were observed.
比較例
比較のため、現在市販されている血液検査用採血管(E
DTA−2Kを血液1プ当、91.2rI9封入されて
いる真空採血管)を用いて、上記実施例と同一条件下で
赤血球数、白血球数、血小板数の検査をおこなった。な
お、試薬添加前の血液はpH7,4、添加後の−は7.
2であった。その結果を同じく下記表に示す。Comparative Example For comparison, blood test blood collection tubes (E
The number of red blood cells, white blood cells, and platelets were tested under the same conditions as in the above example using one volume of DTA-2K blood and a vacuum blood collection tube filled with 91.2rI9. The pH of the blood before addition of the reagent was 7.4, and the pH of the blood after addition was 7.4.
It was 2. The results are also shown in the table below.
[発明の具体的効果]
以上詳述したように、この発明に係わる血液検査用採血
管によれば、採取した血液の声を特定範囲に調整するこ
とにより経時的な血小板の凝集を抑えることができるの
で、赤血球数、白血球数とともに、血小板数の測定をよ
り正確におこなうことが可能となる。[Specific Effects of the Invention] As detailed above, according to the blood test blood collection tube of the present invention, platelet aggregation over time can be suppressed by adjusting the volume of the collected blood to a specific range. This makes it possible to more accurately measure the number of platelets as well as the number of red blood cells and white blood cells.
さらに血液抗凝固剤として酸性のものを使用すれば、酸
の添加が不要となるため、試薬の調製が簡単となり、従
来市販されている採血管と同程度のコストで製造するこ
ともできる。Furthermore, if an acidic blood anticoagulant is used, there is no need to add an acid, which simplifies the preparation of the reagent, and it can be manufactured at a cost comparable to that of conventionally commercially available blood collection tubes.
第1図は本発明に係わる血液検査用採血管の断面図、第
2図および第3図は他の実施iHJ K係わる血液検査
用採血管の断面図である。
図中、!・・・有底管体、2,3.4・・・試薬。
出願人代理人 弁理士 鈴 江 武 彦第1図 第
2図 第3図FIG. 1 is a cross-sectional view of a blood test blood collection tube according to the present invention, and FIGS. 2 and 3 are cross-sectional views of blood test blood collection tubes according to other embodiments. In the diagram! ...bottomed tube, 2,3.4...reagent. Applicant's representative Patent attorney Takehiko Suzue Figure 1 Figure 2 Figure 3
Claims (3)
してなる血液検査用採血管において、該薬品が血液抗凝
固作用と、採取した血液のpHを5.0ないし7.0に
する作用を有することを特徴とする血液検査用採血管。(1) In a blood test blood collection tube comprising a bottomed tube and a drug sealed in the tube, the drug has a blood anticoagulant effect and increases the pH of the collected blood from 5.0 to 7. 1. A blood test blood collection tube characterized by having an action of reducing the amount of blood to .0.
、それにより上記範囲のpHの調整作用を有する特許請
求の範囲第1項記載の血液検査用採血管。(2) The blood test blood collection tube according to claim 1, wherein the drug is a blood anticoagulant and is acidic, thereby having a pH adjustment effect within the above range.
酸又は無機酸を含有するものである特許請求の範囲第1
項記載の血液検査用採血管。(3) Claim 1, wherein the chemical contains an organic acid or an inorganic acid that has a pH adjusting effect within the above range.
Blood test blood collection tube as described in Section 1.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15914486A JPS6315161A (en) | 1986-07-07 | 1986-07-07 | Blood sampling tube for blood test |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15914486A JPS6315161A (en) | 1986-07-07 | 1986-07-07 | Blood sampling tube for blood test |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6315161A true JPS6315161A (en) | 1988-01-22 |
Family
ID=15687213
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP15914486A Pending JPS6315161A (en) | 1986-07-07 | 1986-07-07 | Blood sampling tube for blood test |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6315161A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0575736A3 (en) * | 1992-06-11 | 1994-05-18 | Becton Dickinson Co | Anticoagulant solution |
WO2009115608A2 (en) * | 2008-03-20 | 2009-09-24 | Iti Scotland Limited | Uses of reagents in sample collection and cartridge systems |
CN103323296A (en) * | 2012-03-23 | 2013-09-25 | 南京柯伦迪检测技术有限公司 | Anticoagulant agent match use method for blood platelet function detection |
JP2014228467A (en) * | 2013-05-24 | 2014-12-08 | 栗田工業株式会社 | Anionic polymer concentration measuring method and device |
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JPS5661651A (en) * | 1979-10-09 | 1981-05-27 | Abbott Lab | Method of and apparatus for sampling blood |
JPS62242854A (en) * | 1986-04-15 | 1987-10-23 | Kyoto Ikagaku Kenkyusho:Kk | Method for inhibiting agglutination of platelet of blood |
JPS62276460A (en) * | 1986-05-23 | 1987-12-01 | Kyoto Ikagaku Kenkyusho:Kk | Method for fractionating and measuring blood platelet by activation type |
-
1986
- 1986-07-07 JP JP15914486A patent/JPS6315161A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5661651A (en) * | 1979-10-09 | 1981-05-27 | Abbott Lab | Method of and apparatus for sampling blood |
JPS62242854A (en) * | 1986-04-15 | 1987-10-23 | Kyoto Ikagaku Kenkyusho:Kk | Method for inhibiting agglutination of platelet of blood |
JPS62276460A (en) * | 1986-05-23 | 1987-12-01 | Kyoto Ikagaku Kenkyusho:Kk | Method for fractionating and measuring blood platelet by activation type |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0575736A3 (en) * | 1992-06-11 | 1994-05-18 | Becton Dickinson Co | Anticoagulant solution |
JPH0720122A (en) * | 1992-06-11 | 1995-01-24 | Becton Dickinson & Co | Coagulation preventing solution, separating device using solution thereof and separating method |
US5494590A (en) * | 1992-06-11 | 1996-02-27 | Becton Dickinson | Method of using anticoagulant solution in blood separation |
US5667963A (en) * | 1992-06-11 | 1997-09-16 | Becton Dickinson And Company | Anticoagulant solution for use in blood chemistry-related techniques and apparatus |
WO2009115608A2 (en) * | 2008-03-20 | 2009-09-24 | Iti Scotland Limited | Uses of reagents in sample collection and cartridge systems |
WO2009115608A3 (en) * | 2008-03-20 | 2009-12-03 | Iti Scotland Limited | Uses of reagents in sample collection and cartridge systems |
US8669110B2 (en) | 2008-03-20 | 2014-03-11 | Prabhjyot Dehal | Uses of reagents in sample collection and cartridge systems |
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