JPS63140960A - Detection of object of cell classifying device - Google Patents

Abstract

PURPOSE:To obviate erroneous detection and mis-detection by obtaining the absorbancy waveform by the other single color which is largely different in the absorbancy of only the object to be detected from other cells and dirt in the addition to the absorbancy waveform by the single color and obtaining a difference therebetween. CONSTITUTION:A blood specimen smeared and dyed on slide glass is measured by a photoelectric conversion array mounted to a microscope. The absorbancy waveform (b) by the specific single color at D-D' is first obtd. at this time. Another absorbancy waveform (c) is then obtd. by the other single color which is largely different in the absorbancy of only the object A to be detected from the other cell B and dirt C in comparison with the absorbancy waveform (b). The difference between these two waveform signals is determined and the absorbancy waveform (d) is obtd. The waveform (d) is sliced at a level F-F' and is binary-coded. The influence of the cells and dirt except the object to be detected is thereby eliminated and the erroneous detection and mis-detection are obviated.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a method for detecting an object in a cell sorting device, and in particular, to eliminate false detection and detection errors of objects in an automatic blood image classification device in the field of hematology. The present invention relates to an object detection method for a cell sorting device suitable for.

[Prior art]

Conventionally, in image measurement that involves inputting microscopic images, there has been no example of detecting an object using a plurality of single color information, and detection was performed using only single color information.

In addition, for white blood cell detection '3A,
There is one proposed in Publication No. 94.

[Problem that the invention seeks to solve]

The above-mentioned conventional technology is not a good method because it may cause problems such as false detection or detection errors. This will be explained with reference to FIG. What is obtained in the microscopic field is an example of a blood specimen smeared and stained on a slide glass, and ■ indicates the color in the specimen for the m-color that best represents the characteristics of blood cells in DD' of ■. This is an absorbance waveform. In this example, when trying to detect white blood cells A, slide E-E' is located between red blood cells B and a wide area of white blood cells A, and both are separated to detect white blood cells A.

However, since dust C is unavoidably scattered within the microscope field of view during smearing or staining, dust C is also detected in this example. As a means to eliminate this inconvenience, attention is paid to the area of the garbage C and white blood cells A binarized by the slicer E-E', and the size thereof is determined. In the conventional method, it can be said that the slicer E-E' must be within a wide range of white blood cells A and red blood cells B. Both absorbance waveform @ and slider E-E'
Since there are optical and electrical fluctuation factors, it is extremely difficult to always stably and accurately separate and detect white blood cells A from red blood cells B and waste C using this method. In FIG. 3, the slider E-E' and the absorbance waveform @ vary relative to each other.

When the slicer E-E' reaches the level where it can slice red blood cells B, it means that it has also detected red blood cell B cells that are not the detection target, which causes the inconvenience that the processing capacity of the device is significantly reduced, and vice versa. When E-E' reaches a level that slices a narrow portion of the white blood cells A, it becomes difficult to separate the white blood cells A and the waste C. In some cases, this method has the disadvantage of not characteristically detecting small white blood cells. In either case, this is a fatal drawback for the blood image classification device.

An object of the present invention is to provide an object detection method for a cell classification device that can eliminate false detection and detection errors of detection target cells.

[Means for solving problems]

As shown in Figure 2, the purpose of the above is to compare the conventional absorbance waveform ■ with only a single color and the absorbance waveform This is achieved by obtaining an absorbance waveform O which is the difference between the absorbance waveform obtained from one color and detecting it by converting it into a binary value using a slider FF'.

[Effect]

The absorbance waveform using only a single color is the absorbance waveform that best captures the characteristics of blood cells.In addition, compared to this absorbance waveform, there is another single-color absorbance waveform in which only the detection target has a significantly different absorbance from other cells and dirt. Since the absorbance waveform obtained from the above is obtained and the difference between these two absorbance waveforms is determined, white blood cells, which are the detection target, are clearly extracted because red blood cells and dust are almost completely removed. , This was made into 2ft with a slider and the above 2.
Even slight electrical and optical fluctuations in absorbance waveforms will not cause false detection.

〔Example〕

Hereinafter, one embodiment of the object detection method of the present invention will be described in detail with reference to FIG.

FIG. 1 is an explanatory diagram of the device configuration for explaining one embodiment of the target object detection method of the cell classification device of the present invention. In Figure 2, the rivet head of the microscope G is equipped with a color television camera 1 for capturing microscope images, a semiconductor photoelectric conversion array J for obtaining the absorbance waveform @ shown in Figure 2, and a semiconductor photoelectric conversion array for obtaining the absorbance waveform ○. Array K is the attachment. The output of the semiconductor photoelectric conversion array J is sent to the differential amplifier circuit section, and the output of the f-conductor photoelectric conversion array J is sent to the slicer M.
It is also sent to the semiconductor optical conversion array J in the slicer M.
The slice level corresponding to the span variation of the output is determined. In the differential amplifier circuit section, semiconductor photoelectric conversion array J
, to create the optical intensity waveform plane I, and then take the difference between the two signals to make the absorbance waveform O 1! Toru. Also,
The absorbance waveform (1) is sliced by the output FF' of the slicer M, and the final output is a binary detection signal.

The stage control section N acts on the stage drive section P, and
X, Y, Z stage R on which 14fi book S is placed x,
Control in the y and z directions. When the final output binarized by the slider M determines that the object to be detected has been detected, the stage control unit N moves the object to be detected in the XY direction of the XYZ stage R to the center of the color television camera I. The two directions are controlled in the in-focus direction based on commands from the automatic focus detection section T.

According to this embodiment, the object to be detected can be detected without false detection, which prevents data errors caused by erroneously detecting specific cells, and also eliminates the detection of foreign substances such as dust or other cells, making processing easier. You can speed up the process.

〔Effect of the invention〕

As explained above, according to the present invention, the influence of cells and dust other than those to be detected can be almost eliminated.

This has the effect of enabling accurate and stable object detection without false detection or detection errors.

[Brief explanation of the drawing]

FIG. 1 is a device i! for explaining an embodiment of the object detection method of the cell sorting device of the present invention. ! FIG. 2 is an explanatory diagram of an embodiment of the present invention, and FIG. 3 is an explanatory diagram of a prior art. G...Microscope, Engineering...Color television camera, J, K
...Semiconductor photoelectric conversion array, L...Differential amplifier circuit,
M...slizer, T...automatic focus detection unit, N...stage control unit, P...stage drive unit, R...XY
Z stage, S...Cell specimen.

Claims (1)

[Claims] 1. In a system that performs cell classification by inputting microscopic images, when detecting a detection target based on multiple single color information, the conventional absorbance waveform using only a single color and the detection target based on the absorbance waveform are used. A cell sorting device characterized by obtaining the difference between absorbance waveforms obtained from other single colors in which only objects have absorbances that are significantly different from other cells and dirt, and detecting the difference by converting it into a binary value using a slider. object detection method.