JPS63137671A - Petri dish for holding medium - Google Patents

Abstract

PURPOSE:To enable the automatic smearing of a specimen on a plurality of different media in a Petri dish, by detachably placing a partition plate in the Petri dish. CONSTITUTION:The objective Petri dish for holding a medium is composed of a dish 11 and a cover 12 and has a structure to enable the attaching and detaching of a partition plate 14 for dividing the inside of the dish 11 into plural sections. In the pouring of medium into the dish 11, the partition plate 14 is attached to the dish and each section is filled with a different kind of medium. The partition plate 14 is removed after the solidification of the media. With the partition plate 14 removed from the dish, a specimen can be spirally smeared on the media by rotating the dish 11.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention relates to a culture medium containing petri dish containing a medium (for example, an agar medium, etc.) for culturing microorganisms in testing (separation, identification, etc.) of microorganisms. The present invention relates to a culture medium-accommodating Petri dish in which different types of culture media can be prepared as a flat fractionated culture medium in a Petri dish.

BACKGROUND OF THE INVENTION A conventional petri dish for storing a general culture medium consists of a Petri dish, for example, about 93 mm in diameter, which houses an agar medium or the like for culturing microorganisms, and a lid that covers an opening on the top surface of the Petri dish. Then, a prescribed amount of a solution of agar powder dissolved in hot water was injected into the Petri dish, and the solution was cooled and solidified to prepare a culture medium. In order to culture microorganisms using the amended medium, a desired amount of a sample is taken using a platinum loop or the like and smeared on the surface of the medium. However, in this case, in order to smear the above-mentioned sample onto different types of media, it was necessary to prepare different media for each Petri dish. Therefore, a large number of petri dishes are required, and a large number of petri dishes must be smeared with the sample one by one, which is time consuming and costly.

To deal with this, conventionally, as shown in FIGS. 20 and 21, a partition wall 2 is erected inside the Petri dish 1 to divide the space for accommodating the culture medium into a plurality of compartments 1, for example, two compartments. A different type of culture medium 3a is provided in each compartment divided by this partition wall 2.
, 3b is injected into a culture medium containing petri dish called a fractionation petri dish. In addition, the second
In FIG. 0, reference numeral 4 is a lid that covers the top opening 5 of the Petri dish 1. In this case, since a plurality of different culture media can be prepared in - number of petri dishes, the number of petri dishes can be reduced.

Problems to be Solved by the Invention However, in such a conventional medium-accommodating Petri dish, the partition wall 2 is fixedly erected inside the Petri dish 1, and the surface of the partition wall 2 is fixed at the boundary between the respective mediums 3a and 3b. Since it protrudes further upward, manually insert the above medium 3a.
, 3b, but when attempting to mechanize this smearing operation, the partition wall 2 becomes an obstacle. Therefore, it is not only difficult to automate the smearing of samples on a plurality of heterogeneous media 3a and 3b in one Petri dish 1, but also difficult to put this into practical use.

In addition, since a partition wall 2 protrudes between the medium 3a and 3b, a certain amount of sample taken with a platinum loop etc. is transferred to each medium 3a and 3b (sometimes the medium is divided into three or more types). ) could not be smeared continuously in one stroke. Therefore, heterogeneous media 3a, 3
It was not possible to average the samples and smear them on b, making it difficult to uniformly test the heterogeneous media 3a and 3b within a certain area.

Therefore, an object of the present invention is to provide a culture medium containing petri dish that can solve such problems.

Means for Solving the Problems The means of the present invention for solving the above-mentioned problems is a medium accommodating medium comprising a Petri dish containing a medium for culturing microorganisms and a lid covering the top opening of the Petri dish. In the petri dish, a partition plate is removably incorporated inside the Petri dish to divide the space for accommodating the culture medium into a plurality of compartments so that the injected culture medium does not mix. A plate is attached to the inside of the Petri dish, and the partition plate is removed after the injected medium has solidified.

Embodiments Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is a central cross-sectional view showing a culture medium containing Petri dish according to the present invention, and FIG. 2 is a plan view showing the Petri dish. This medium-accommodating petri dish accommodates a medium suitable for each microorganism or in accordance with the purpose in order to culture the target microorganism, and is composed of a Petri dish 11 and a lid 12.

The Petri dish 11 accommodates a medium for culturing microorganisms therein, and is formed into a circular dish shape, for example, with a diameter of about 91 mm, with an outer peripheral edge 13 standing upright around the periphery. It is said to be made of glass or plastic. Here, inside this Petri dish 11, a partition plate 14 is removably incorporated. This partition plate 14 divides the space for accommodating the medium into a plurality of compartments, for example, two compartments, so that the medium injected therein does not mix, and is installed in a linear manner. That is, on the inner bottom surface of the Petri dish 11, as shown in FIG. 2, the holes extend along the diameter direction, and as shown in FIG. Two supporting members 15.15 are provided, and in the gap between these two supporting members 15.15, a thin plate-shaped partition plate 14 extending in the diametrical direction of the Petri dish 11 and at a height slightly lower than its outer peripheral edge 13 is provided. are removably mated. Note that the support member 1
5.15, as shown in FIG.
, 15' are provided.

The means for preventing this mixing is the mixing preventing piece 15'.
, 15', for example, the outer peripheral edge 13 of the Petri dish 11
A vertical groove may be formed in the partition plate 14, and the partition plate 14 may be made slightly elongated, and both ends of the partition plate 14 may be fitted into the groove. Alternatively, flexible The contact piece may be extended and provided, and this contact piece may be brought into close contact with the inner peripheral surface of the outer peripheral edge 13. Further, the partition plate 14 is
As will be described later, a release agent such as silicone is applied to the surface of the medium to facilitate removal after solidification. When injecting the culture medium into the Petri dish 11, the partition plate 14 is fitted and attached to the gap between the support members 15 and 15, and after the injected culture medium has solidified, the partition plate 14 is designed to be removed.

The outer peripheral edge 13 of the Petri dish 11 is covered with a lid 12. This lid 12 is similar to the Petri dish 11.
It covers the top opening 16 and has a circular shape that is slightly larger than the outer diameter of the Petri dish 11.A downward skirt part 17 is provided around it, and like the Petri dish 11 described above, it is made of glass or It is said to be made of plastic.

Next, the use of the culture medium containing petri dish configured as described above will be explained. First, as shown in FIGS. 1 and 2, a support member 15.
A partition plate 14 is fitted and attached to the gap between 15 and 15, and the interior of the Petri dish 11 is divided into, for example, two sections by the partition plate 14. At this time, the gap between the supporting members 15 and 15 may be filled with a mixing prevention agent such as silicone grease. Next, a different type of culture medium in the form of a solution of, for example, agar powder dissolved in hot water is injected into each of the areas A and B divided by the partition plate 14 using a nozzle or the like. At this time, each area A.

The heterogeneous culture medium in the form of a solution injected into the Petri dish 11 is transported by the supporting member 15.15 at the bottom of the Petri dish 11, by the partition plate 14 at the middle and upper part of the Petri dish 11, and by the partition plate 14 at the middle and upper part of the Petri dish 11. Both ends are separated by mixing prevention means such as mixing prevention pieces 15', 15' of the support member 15 to prevent mixing. Next, after a prescribed amount of different types of culture medium has been injected in this manner, the top opening 16 of the Petri dish 11 is covered with a lid 12, as shown in FIG. Then, after a predetermined period of time has elapsed, the medium in the form of a top solution solidifies.

Next, once the medium has solidified in this way, open the lid 12 in the direction of arrow C, as shown in FIG.

Grasp the top end of the partition plate 14 and pull it in the direction of the arrow.
The partition plate 14 is removed from the support member 15.15. In addition, at this time, the above l! 12 can be opened by vacuum suction using a robot hand or the like, and the partition plate 14 can also be removed by gripping the chuck part of the robot hand or the like. As a result, as shown in FIG. 5, a groove 18 is formed at the boundary, and different types of culture media 19a and 19b divided into four sections by the groove 18 are placed in one Petri dish 11.
・Prepared as a plate-shaped fractionated medium. Therefore, unlike in the past, there is no protrusion such as the partition wall 2 (see FIGS. 20 and 21) at the boundary between different types of media, so as shown in FIG. While rotating the platinum loop with the sample attached to the medium 19a,
19b and move it in the radial direction,
The samples can be serially spread in a spiral onto different types of media 19a, 19b, respectively.

7 and 8 are a central cross-sectional view and a plan view showing a modified example of the structure in which the partition plate is incorporated. In this modification, a support member 15 is provided on the inner bottom surface of the Petri dish 11, extending along the diameter direction as shown in FIG. 8, and protruding from the bottom surface at an appropriate height as shown in FIG. and a partition plate 14'
The partition plate 14' is formed into a somewhat thick plate shape, and has a bifurcated portion 20.20 on the bottom and both end surfaces thereof having a groove into which the support member 15 can fit.
The support member 15 is removably assembled by fitting the groove between 0 and 0 into the single support member 15 described above. As shown in FIG. 8, mixing prevention pieces 15' are provided at both ends of the support member 15 with an appropriate width and extend up to the height of the outer peripheral edge 13.

In this case as well, the partition plate 14' is similar to that shown in FIG.
It can be removed by pulling in the direction of the arrow.

FIGS. 9 and 10 are a central cross-sectional view and a plan view showing other modified examples of the structure in which the partition plate is incorporated. This variant has an inner bottom surface of the Petri dish 11 that extends diametrically as shown in FIG.

As shown in FIG. 9, a base member 21 is provided that protrudes from the bottom surface at an appropriate height, and a thin film-like material that can be torn by applying external force between the base member 21 and the partition plate 14' located above the base member 21 is provided. By connecting with a connecting piece 22, it is installed in a detachable manner.

As shown in FIG. 10, mixing prevention pieces 21' are provided at both ends of the base member 21 with an appropriate width and rising up to the height of the outer peripheral edge 13. In this case as well, as shown in FIG. 4, by pulling the partition plate 14' in the direction of the arrow, the connection piece 22 is torn and the partition plate
' can be removed. Note that the connecting piece 22 may be directly connected to the bottom surface of the Petri dish 11 and the inner circumferential surface of the outer peripheral edge 13 without providing the base member 21 and the mixing prevention piece 21'.

Although not shown in the drawings, the structure for incorporating the partition plate into the Petri dish 11 is not limited to the above structure, but may be an appropriate combination of the embodiments shown in FIGS. 1, 7, and 9 above. There may be.

FIG. 11 is a central cross-sectional view showing a second embodiment of the present invention. In this embodiment, an engaging part 23 for removal is provided at the upper end of the partition plate 14 incorporated into the Petri dish 11, and
On the back side of the lid 12, there is an engagement holding portion that engages with the engagement portion 23 of the partition plate 14 at a portion that matches the engagement portion 23 of the partition plate 14 when the lid 12 is placed on the Petri dish 11. 24. As shown in FIG. 12, the engaging portion 23 has rectangular engaging holes 2 at the upper ends of both sides of the partition plate 14, for example.
It is formed by drilling 5. In addition, the engagement holding portion 24
As shown in FIGS. 11 and 13, for example, an L-shaped hanging member is attached downward to a location corresponding to the engagement hole 25.25 of the partition plate 14 on the back surface of n12, and the lower end thereof is attached. The horizontal tongue pieces are formed so as to face each other inwardly so as to be able to fit into the above-mentioned engagement holes 25, 25, respectively.

In this embodiment, as in the first embodiment described above,
First, the partition plate 14 is fitted into the gap between the supporting members 15 and 15 provided on the Petri dish 11, and then the different species in the form of a solution are placed in the respective areas A and B divided by the partition plate 14. In this state, as shown in FIG. 13, cover 12 is placed on the top surface of Petri dish 11.
cover.

At this time, initially, the lid 12 is placed on the lid 12 with its position shifted so that the engagement holding parts 24, 24 of the lid 12 do not fit into the engagement holes 25, 25 of the partition plate 14. After a predetermined period of time has elapsed and the solution-like medium has solidified, the lid 12 is rotated in a certain direction, for example, clockwise, so that the engaging holding portion 24 of the lid 12 is rotated as shown in FIG. .24 into the engagement holes 25, 25 of the engagement portions 23, 23 of the partition plate 14, as shown in FIG.
Open like. Then, since the engagement holding part 24.24 on the back side of n12 and the engagement part 23.23 of the partition plate 14 are fitted together as described above, the partition plate 14 is opened at the same time as the opening operation in the direction of the arrow F. can be removed from the support member 15.15. Therefore, in this case, only the action of opening the lid 12 is sufficient, and a special action of removing the partition plate 14 can be made unnecessary. Note that the rotation and opening of the lid 12 at this time can be performed by vacuum suction using a robot hand or the like. In addition, in Fig. 11, the first
Although shown as being applied to the embodiment shown in the figure,
The invention is not limited to this, and can be similarly applied to the modification shown in FIG. 7 or FIG. 9.

FIG. 15 is a central cross-sectional view showing a third embodiment of the present invention, and FIG. 16 is a plan view thereof. In this embodiment, a support member 1 provided on the Petri dish 11 is provided on the back side of the lid 12'.
A partition plate 14 that extends and fits into the gap between 5 and 15 is integrally provided, and the lid 12' includes the partition plate
Culture medium inlets 26 and 27 are bored at appropriate positions corresponding to the two areas A and B divided by 4, and a lid 12' is placed on the top surface of the Petri dish 11, and the lower end of the partition plate 14 is opened. is fitted into the gap between the support members 15 and 15, and the two are assembled in advance. In this embodiment, first, as shown in FIG. 15, a solution is injected into the respective regions A and B divided by the partition plate 14 from the culture medium inlet 26, 27 formed in the lid 12'. Arrow G shows a different type of culture medium using a nozzle, etc.

Inject as shown in H. Next, when a specified amount of different types of culture medium is injected in this way, as shown in Figure 17,
Culture medium inlet 26 and 27 are each sealed with sealing tape 28.2
Seal with 9 or a blind stopper.

Then, after a predetermined period of time has passed, the solution-like medium solidifies. When smearing the sample in this condition,
As shown in FIG. 18, when the lid 12' is opened in the direction of the arrow, since the partition plate 14 is integrally provided on the back side of the lid 12', the opening movement in the direction of the arrow is performed. At the same time, the partition plate 14 can be removed from the support member 15.15.

Therefore, in this case as well, it is only necessary to open the lid 12', and there is no need for a special operation to remove the partition plate 14. Note that the opening of the lid 12' at this time is as follows:
This can be carried out by vacuum suction using a robot hand or the like.

In the above description, the space for accommodating the culture medium in the Petri dish 11 is divided into three sections by the partition plate 14, but the present invention is not limited to this.
As shown in FIG. 9, the partition plate 14 may be formed into a trident shape to have three sections, or may have four or more sections. Further, although the Petri dish 11 and the lid 12 are illustrated as circular, they are not limited to this, and may have a rectangular or other shape, for example.

Effects of the Invention Since the present invention is configured as described above, the partition plate 14 is attached to the inside of the Petri dish 11 when a medium is injected into the Petri dish 11, and after the injected medium is solidified, the partition plate 14 is attached to the inside of the Petri dish 11. By removing the plate 14, different types of culture media 19a and 19b can be prepared in one Petri dish 11 as flat fractionated media. Therefore, unlike the conventional example shown in FIG. 20, there is no protrusion such as the partition wall 2 at the boundary between the different types of culture media 3a and 3b, and for example, as shown in FIG.
By rotating the Petri dish 11 in the direction of arrow E, samples can be continuously smeared in a spiral manner onto different types of media 19a and 19b, respectively. From this, it is possible to automate the smearing of samples on a plurality of heterogeneous media in one Petri dish 11 and save labor.

In addition, since it is possible to smear a certain amount of sample taken with a platinum loop etc. onto each of multiple different types of media in a single stroke, the sample can be averaged and smeared onto each of the multiple different types of media. It is possible to uniformly test different types of culture media within a certain area.

[Brief explanation of the drawing]

Fig. 1 is a central cross-sectional view showing a petri dish containing a medium according to the present invention, Fig. 2 is a plan view showing the petri dish, and Fig. 3 is a cross-sectional view showing a state in which a medium is poured into the petri dish and the lid is closed. Fig. 4 is a cross-sectional explanatory diagram showing the state in which the lid is opened and the partition plate is removed, Fig. 5 is a cross-sectional explanatory diagram showing the state in which a flat plate-shaped fractionated medium is prepared, and Fig. 6 is a cross-sectional explanatory diagram showing the state in which a flat plate-shaped fractionated medium is prepared. FIGS. 7 and 8 are central cross-sectional views and plan views showing modified examples of the structure in which the partition plate is incorporated, and FIGS. 9 and 10 are views of the partition plate. 11 is a central cross-sectional view and a plan view showing another modified example of the built-in structure; FIG. 11 is a central cross-sectional view showing the second embodiment of the present invention; FIG. 12 is a front view showing the partition plate; 13 and 14 are cross-sectional explanatory diagrams showing the usage state of the second embodiment, FIG. 15 is a central cross-sectional view showing the third embodiment, FIG. 16 is a plan view thereof, and FIG. 17 and FIG. Fig. 18 is a cross-sectional explanatory diagram showing the usage state of the third embodiment, Fig. 19 is a plan view showing a modification of the division of the Petri dish into multiple compartments, and Fig. 20 is a central cross-sectional view showing a conventional medium-accommodating Petri dish. 21 is a plan view showing the Petri dish. 11... Petri dish, 12, 12'... Lid, 14
゜14', 14'...Partition plate, 15...Supporting member, 16...Top opening, 18...Concave groove at boundary. 19a, 19b... fractionated heterogeneous medium, 23...
・Engagement part, 24...Engagement holding part, 26.27...
- Culture medium inlet, 28, 29... sealing tape. Applicant Elmex Co., Ltd. Sogo Biomedical Research Institute Co., Ltd. Figure 2 Figure 3 II Figure 8 Figure 10 Figure I9 Figure 11 Figure 13 Figure 14 Section fm

Claims (2)

[Claims] (1) In a medium-accommodating Petri dish consisting of a Petri dish that houses a medium for culturing microorganisms and a lid that covers the top opening of the Petri dish, a space for housing the medium is injected into the inside of the Petri dish. A partition plate that divides the culture medium into a plurality of compartments is removably incorporated to prevent mixing of the culture medium, and when the culture medium is injected into the Petri dish, the partition plate is attached to the inside of the Petri dish, and the injected culture medium is solidified. A petri dish containing a culture medium, characterized in that the partition plate can be removed afterwards. (2) The upper end of the partition plate to be incorporated into the Petri dish is provided with an engaging part for removal, and the engaging part of the partition plate is attached to the back side of the lid when the lid is placed on the Petri dish. Claim 1, characterized in that an engagement holding portion that engages with the engagement portion of the partition plate is provided at a portion that matches the partition plate, and the partition plate is removed by opening the lid. The culture medium-accommodating petri dish according to item 1.