JPS6298253A - Liquid chromatograph - Google Patents
Liquid chromatographInfo
- Publication number
- JPS6298253A JPS6298253A JP23731685A JP23731685A JPS6298253A JP S6298253 A JPS6298253 A JP S6298253A JP 23731685 A JP23731685 A JP 23731685A JP 23731685 A JP23731685 A JP 23731685A JP S6298253 A JPS6298253 A JP S6298253A
- Authority
- JP
- Japan
- Prior art keywords
- column
- diameter
- inside diameter
- cylindrical column
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6004—Construction of the column end pieces
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Description
【発明の詳細な説明】
〔発明の利用分野〕
本発明は液体クロマトグラフに係り、特に改良された分
離カラムを備えた液体クロマトグラフに関する。DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to liquid chromatographs, and more particularly to liquid chromatographs with improved separation columns.
液体クロマトグラフの分離・検出能向上のために、固定
相を構成する充填剤の高性能化が進められ、各種充填剤
（吸着剤やイオン交換体等）の粒子が微細化（直径３μ
ｍ以下）されてきた。また、溶離液の使用量を小さくす
るために分離カラムの内径も小さくなる方向にある。こ
のようになってくると、充填剤の性能はカラムの構造、
特に、送液チューブとの接続部にできるデッドボリュー
ムの大きさに左右される。In order to improve the separation and detection capabilities of liquid chromatographs, the performance of the packing materials that make up the stationary phase has been improved, and the particles of various packing materials (adsorbents, ion exchangers, etc.) have become finer (3μ in diameter).
m or less). Furthermore, in order to reduce the amount of eluent used, the inner diameter of separation columns is also becoming smaller. When this happens, the performance of the packing material depends on the structure of the column.
In particular, it depends on the size of the dead volume created at the connection with the liquid feeding tube.
微粒子充填剤の性能を損なわない分離カラムの構造とし
て、特公昭５６−４４３７６号公報に示されたものが知
られている。しかし、充填剤がさらに微粒子化した場合
や分離カラム内径を微小化した場合に生じる、カラム自
身による試料成分バンド（同一成分よりなる分離層）の
拡がりよりもカラム外の拡がりの方が分離効率に支配的
であるという点については配慮されていない。As a structure of a separation column that does not impair the performance of the fine particle packing material, the structure shown in Japanese Patent Publication No. 44376/1983 is known. However, when the packing material becomes finer particles or when the inner diameter of the separation column is made smaller, the spread of the sample component band (separation layer made of the same components) outside the column is more likely to affect the separation efficiency than the spread of the sample component band (separation layer made of the same components) caused by the column itself. There is no consideration given to the fact that it is dominant.
本発明は微粒子（例えば直径３μｍ以下）充填剤を充填
するのに適するように１分離カラムの構造を改良し、分
離、検出性能の高精度化をはかることができる液体クロ
マトグラフを提供することにある。The present invention aims to provide a liquid chromatograph that improves the structure of a separation column so that it is suitable for filling with fine particles (for example, diameters of 3 μm or less), and that improves the accuracy of separation and detection performance. be.
本発明は、混合物試料の成分分離をする分離カラムを備
え、上記分離カラムが充填剤を収容した円筒状カラムと
、この円筒状カラムの両端付近にあって着脱可能な中空
ボルトを備えており、上記中空用ボルトは流体を通過さ
せ粒状物の通過を阻止する円板状物質および上記円筒状
カラムの入口側のほぼ中心に移動相液を導くための孔を
有するものである液体クロマトグラフに適用される。The present invention includes a separation column for separating components of a mixture sample, and the separation column includes a cylindrical column containing a packing material, and removable hollow bolts located near both ends of the cylindrical column, The above-mentioned hollow bolt is applicable to a liquid chromatograph, which has a disk-shaped substance that allows fluid to pass through but blocks the passage of particulate matter, and a hole that guides the mobile phase liquid to approximately the center of the inlet side of the cylindrical column. be done.
本発明では、上記孔の内径が送液チューブの外径より大
であり、上記円板状物質の実質的フィルタ部分の径が上
記送液チューブの内径以上であって上記円筒状カラムの
内径以下であることを特徴とする。In the present invention, the inner diameter of the hole is larger than the outer diameter of the liquid feeding tube, and the diameter of the substantial filter portion of the disk-shaped substance is greater than or equal to the inner diameter of the liquid feeding tube and less than or equal to the inner diameter of the cylindrical column. It is characterized by
以下、本発明の一実施例を第１図により説明する。微粒
子充填剤３は円筒状カラム４内にディスク５により保留
されている。中空ボルト２はサンプルを分離カラム中の
充填剤に導く送液チューブ１の外径と等しいかやや大き
い孔を有しており、送液チューブ１は直接ディスク５と
圧着した状態となっている。ディスク５はステンレス鋼
製の焼結フィルタあるいはガラス繊維、多孔性高分子膜
フィルタ等が用いられる。ディスク５は使用時には、円
筒状カラム４と中空ボルト２及び送液チューブ１の圧力
により圧縮され、フィルタ中の細孔がつぶされるため、
実質的なフィルタ部分の径ｄｆは送液チューブ１の内径
ｄｉと円筒状カラム４の内径ｄｃの間に存在することに
なる。また。An embodiment of the present invention will be described below with reference to FIG. The particulate filler 3 is retained in a cylindrical column 4 by a disk 5. The hollow bolt 2 has a hole that is equal to or slightly larger than the outer diameter of the liquid feeding tube 1 that leads the sample to the packing material in the separation column, and the liquid feeding tube 1 is directly pressed against the disk 5. As the disk 5, a sintered filter made of stainless steel, a glass fiber filter, a porous polymer membrane filter, or the like is used. During use, the disk 5 is compressed by the pressure of the cylindrical column 4, hollow bolt 2, and liquid feeding tube 1, and the pores in the filter are crushed.
The substantial diameter df of the filter portion is between the inner diameter di of the liquid feeding tube 1 and the inner diameter dc of the cylindrical column 4. Also.
円筒状カラム４の内径ｄｃは送液チューブ１の内径ｄｉ
の１．１〜１．５倍以内になるように選択される。以上
、説明したように、本実施例によればサンプルは、必要
最小限の面積のフィルタ部分を介して直接カラム充填剤
へ導かれるため、カラム外での試料成分のバンドの拡が
りを小さくできるという効果がある。さらに、送液チュ
ーブ１の内径と円筒状カラム４の内径の差が小さいため
送液チューブ１より流入したサンプルは渦流を起こすこ
となくカラム内を通過する。The inner diameter dc of the cylindrical column 4 is the inner diameter di of the liquid feeding tube 1.
is selected to be within 1.1 to 1.5 times. As explained above, according to this example, the sample is guided directly to the column packing material through the filter part with the minimum necessary area, so it is possible to reduce the band spread of sample components outside the column. effective. Further, since the difference between the inner diameter of the liquid feeding tube 1 and the inner diameter of the cylindrical column 4 is small, the sample flowing from the liquid feeding tube 1 passes through the column without causing a vortex.
そのため、本実施例によれば、試料成分のカラム内外で
の拡散を小さくできるため、液体クロマトグラフィーの
分離、検出の高性能化が達成できるという効果がある。Therefore, according to this embodiment, the diffusion of sample components inside and outside the column can be reduced, so that it is possible to achieve high performance separation and detection in liquid chromatography.
本発明によれば、試料成分のバンドの拡がりを小さくで
きるので、液体クロマトグラフィーの分離能及び検出能
の高度化が達成できる。According to the present invention, the spread of bands of sample components can be reduced, so that the separation ability and detection ability of liquid chromatography can be improved.
第１図は本発明の一実施例を示す分離カラムの断面図で
ある。FIG. 1 is a sectional view of a separation column showing one embodiment of the present invention.
Claims (1)
記分離カラムが充填剤を収容した円筒状カラムと、この
円筒状カラムの両端付近にあつて着脱可能な中空ボルト
を備えており、上記中空用ボルトは流体を通過させ粒状
物の通過を阻止する円板状物質および上記円筒状カラム
の入口側のほぼ中心に移動相液を導くための孔を有する
ものである液体クロマトグラフにおいて、上記孔の内径
が送液チューブの外径より大であり、上記円板状物質の
実質的フィルタ部分の径が上記送液チューブの内径以上
であつて上記円筒状カラムの内径以下であることを特徴
とする液体クロマトグラフ。 ２上記円筒状カラムの内径は、上記送液チューブの外径
以下であり、かつ、上記送液チューブの内径の１．１〜
１．５倍であることを特徴とする液体クロマトグラフ。[Scope of Claims] 1. A separation column for separating the components of a mixture sample, the separation column comprising a cylindrical column containing a packing material, and removable hollow bolts located near both ends of the cylindrical column. The hollow bolt has a disc-shaped substance that allows fluid to pass through and blocks particulate matter from passing through, and a hole that guides the mobile phase liquid to approximately the center of the inlet side of the cylindrical column. In the chromatograph, the inner diameter of the pore is larger than the outer diameter of the liquid feeding tube, and the diameter of the substantial filter portion of the disc-shaped substance is greater than or equal to the inner diameter of the liquid feeding tube and less than or equal to the inner diameter of the cylindrical column. A liquid chromatograph characterized by: 2. The inner diameter of the cylindrical column is less than or equal to the outer diameter of the liquid feeding tube, and is 1.1 to 1.2 times the inner diameter of the liquid feeding tube.
A liquid chromatograph characterized by a magnification of 1.5 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23731685A JPS6298253A (en) | 1985-10-25 | 1985-10-25 | Liquid chromatograph |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23731685A JPS6298253A (en) | 1985-10-25 | 1985-10-25 | Liquid chromatograph |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6298253A true JPS6298253A (en) | 1987-05-07 |
Family
ID=17013560
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP23731685A Pending JPS6298253A (en) | 1985-10-25 | 1985-10-25 | Liquid chromatograph |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6298253A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5527604A (en) * | 1993-08-06 | 1996-06-18 | Mitsubishi Denki Kabushiki Kaisha | Metal base board and electronic equipment using the same |
-
1985
- 1985-10-25 JP JP23731685A patent/JPS6298253A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5527604A (en) * | 1993-08-06 | 1996-06-18 | Mitsubishi Denki Kabushiki Kaisha | Metal base board and electronic equipment using the same |
| US5578367A (en) * | 1993-08-06 | 1996-11-26 | Mitsubishi Denki Kabushiki Kaisha | Metal base board and electronic equipment using the same |
| US5670241A (en) * | 1993-08-06 | 1997-09-23 | Mitsubishi Denki Kabushiki Kaisha | Metal base board and electronic equipment using the same |
| US5820972A (en) * | 1993-08-06 | 1998-10-13 | Mitsubishi Denki Kabushiki Kaisha | Metal base board and electronic equipment using the same |
| US5834101A (en) * | 1993-08-06 | 1998-11-10 | Mitsubishi Denki Kabushiki Kaisha | Metal base board and electronic equipment using the same |
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