JPS6279838A - Shaker used for tissue culture of vegetables and animals - Google Patents

Abstract

PURPOSE:To obtain the titled shaker which permits imposition of many culture vessels by supporting a culture vessel imposing plate which is supported on an eccentric shaft and is not provided with vessel fixing means between said shaft and a base plate by flexible supports and horizontally rotating the plate by the rotation of the eccentric shaft. CONSTITUTION:A bed plate 2 is extended on a machine frame 1 to constitute the base plate. A bearing 5, a revolving shaft 6 and the eccentric shaft 7 are provided on the plate 2 and are integrally constituted. The culture vessel imposing plate 8 is loosely fitted to the top end of the shaft 7 to transmit the rotation of the shaft 6. The plate 8 is a flat plate and fixing means to fix the culture vessels are not provided thereon. Supporting rods 12 are provided on the rear side of the four corners. The rods 12 have the flexibility enough to curvilinearly rotate with the rotation of the shaft 6. The eccentric shaft 7 makes the circular motion having a radius around the eccentric shaft if the shaft 6 is rotated. The plate 8 makes horizontal motion in synchronization with the circular motion. The adjacent culture vessels do not, therefore, collide against each other.

Description

[Detailed Description of the Invention] (Industrial Application Field) This invention relates to a rotary shaking culture machine suitable for culturing plant or animal tissue, and its purpose is to reduce the area of the same culture vessel mounting table. The present invention provides a shaking device that can accommodate a larger number of culture vessels.

(Prior art) Conventionally, when culturing microorganisms, plants, animal tissues, etc. aerobically, an aerated agitation culture device, a test sound shaking culture machine,
Various types of equipment are used, such as a reciprocating shaking culture machine, a single-rotation shaking culture machine, and these machines are designed to appropriately supply oxygen depending on the purpose of each culture. Among these, the rotary shaking culture machine is for example
As described in No. 4, a culture container is fixed to an eccentric rotating body rotated by a motor, and is made to move along a direction guided by a guide rod.

When explanting or animal tissue culture is performed using the above-mentioned shaking machine, vigorous shaking may damage the cells, so please do not exceed 120 rpm.
Rotational speeds around pm are often used, and in order to cope with the shaking, the culture container is fixed with a fixture provided on the imaging plate to ensure a predetermined amplitude and prevent it from moving.

(Problems to be solved by the invention) As mentioned above, culture vessels are always fixed on the shaking plate with fixtures, but installing the fixtures occupies a considerable area on the shaking plate. , reduce the number of culture vessels placed. For this reason, when testing using the same shaking machine, it is necessary to limit the test plan or to conduct the test in several batches in order to increase the test density. Decrease test accuracy or increase experimental error. Also, the installation of fixtures has the disadvantage of making the damage machine expensive.

(Means for Solving the Problems) As a result of intensive research to solve the above problems, the inventor installed an eccentric shaft on the rotating shaft of a single-rotation shaker, and used it for culture without fixing devices. Place the container mounting plate rotatably,
A culture vessel can be placed on the entire surface, and at each corner of the culture vessel mounting plate, a support having appropriate flexibility to rotate in response to the rotation of the eccentric shaft is provided between the plate and the base. The problem was solved by making the culture container mounting plate move horizontally and circularly by supporting it and rotating the eccentric shaft so that the culture container can obtain a predetermined shaking effect even without a fixing device.

(Function) In the shaker of the present invention, when the rotating shaft is driven by the motor, the eccentric shaft above the rotating shaft also rotates, and this rotation rotates the culture vessel mounting plate. At this time, each corner of the culture vessel mounting plate is supported by a linear support having appropriate flexibility, such as a hard steel wire SWB, so that the support is bent in accordance with the rotation of the eccentric shaft. Therefore, the entire culture container mounting plate moves in the same circular motion, resulting in horizontal movement without vertical movement. This circular motion uniformly moves the medium in the culture container and supplies oxygen without damaging the culture.

(Example) An example of implementation will be described below with reference to the drawings. In the figure, reference numeral 1 denotes a machine frame, which is made of steel such as molded steel and has a rectangular shape.A floor plate 2 is stretched over the frame, and the frame as a whole constitutes a base. A vertical motor 3 is fixed to one side of the floor plate 2, and a variable speed reducer 4 is provided on the motor 3 so that the rotation speed can be adjusted to a desired speed. A bearing 5 is fixed at the center of the floor plate 2 with an upward opening, and the rotation 41] 6 is rotatably supported in the vertical direction by the bearing 5. The upper end of this rotating shaft 6 is connected to an eccentric shaft 7 at an eccentric position as shown in FIG.
The structure is formed integrally. The upper end of the eccentric @7 is loosely fitted into a bearing 9 fixed to the central part of the back side of the culture container mounting plate 8, so that the rotation of the one-rotation shaft 6 is transmitted via the eccentric shaft 7.

Further, the culture container mounting plate 8 is flat, and there are no culture container fixing devices provided thereon as in the past, and there are four corners on the back side of the culture container mounting plate 8 in order to keep it parallel to the floor plate 2 and horizontal. A support rod fixture 10 is provided, and a support rod 12 is provided between this and support rod fixtures 11 provided at the four corners of the corresponding floorboard 2. The support rod 12 is a wire or rod made of, for example, hard steel wire, and has sufficient flexibility to bend and rotate its upper end in response to the rotation of the eccentric shaft 7. Further, a pulley 13 is provided on the rotating shaft 6 side for driving the rotation +11]6 by the motor 3, and a pulley IJ-14 is provided on the speed reducer 4 side, and a belt 15 is stretched between them. As a result, due to the rotation @6, the eccentric 11117 performs a circular motion with the eccentric width as a radius, and in synchronization with this circular motion, the culture container mounting plate 8 and the support rods 12 at the four corners. ...The upper end performs horizontal circular motion. At this time, if the support rod 12 is too flexible, it will swing on its own and will not synchronize with the rotation of the eccentric shaft 7, so the culture container mounting plate 8 will swing up and down, and the flask 16 will be placed on it. However, it cannot be fixed without a fixing device, and the stirring effect differs for each flask 16, making it impossible to supply oxygen under constant conditions. If the flexibility of the support rod 12 is too low, the four corners will be fixed and smooth circular movement will not be possible.

Therefore, the material, thickness, and length of the support rod 12 are 9 rotations.

It is necessary to calculate and determine the buckling point of the flexible body sufficient to cause a circular motion corresponding to the rotation of the mounting plate, considering the weight placed on the mounting plate, etc.

Now, to explain the above device using a design example, 1 machine frame 1 has 12
A frame of 1200 ma x 900 ma was made of a 0 m x 6.5 ma x 6 ms mouth type steel, and a steel floor plate 2 was placed on top of it. The speed reducer 4 is variable from 12 to 120 rpm, and the rotating shaft 6 has a diameter of 25 mx, an eccentric shaft diameter of 4 □, and the culture vessel mounting plate 8 is made of steel plate with a diameter of 1200 m JX9.
The support rod 12 is made of hard steel wire SWB with a diameter of 5.
The length was 300 mm. 391 50CC flasks were placed on this device, and cymbidium proliferators were stored at 22°C for 2 hours.
Shaking culture was carried out at 100 to 12 rpm for 4 hours, but no collision occurred between adjacent flasks, and shaking culture could be carried out with stable horizontal rotational motion.

(Effects) This invention uses a 9-rotation shaking culture machine to move the culture container mounting plate in a horizontal circular motion, and by simply placing the culture container on top of the plate, culture can be performed without causing any collision between the culture containers. The entire surface of the plate can be used effectively, and 50% more culture vessels can be placed than in conventional devices. In addition, the support rod is inexpensive and can significantly reduce manufacturing costs.The outer structure is simple, so there are fewer failures, and there are no fixing devices to place or remove the culture container on the mounting plate. It has many advantages, such as being simple and easy to work with, and is particularly useful for tissue culture of plants and animals, as it provides adequate oxygen supply without damaging tissues.

[Brief explanation of drawings]

FIG. 1 is a side view of an example of the invention, FIG. 2 is a partially cutaway plan view, and FIG. 3 is a side view of the rotating shaft. 2... Floor plate 3... Motor 6... Rotating shaft 7
...Eccentric shaft 8...Culture container mounting plate 12...Support rod

Claims (1)

[Claims] An eccentric shaft is integrally provided on the upper part of the rotating shaft, and a plate-shaped culture container mounting plate without a container fixing device is rotatably supported at the upper end of the eccentric shaft, and each corner of the culture container mounting plate is is used for tissue culture of plants and animals, characterized in that a support having appropriate flexibility is provided between the base and the base, and the culture vessel mounting plate is horizontally rotated by rotation of an eccentric shaft. Shaking machine.