JPS6274280A - Cultivation of alga of genus spirulina - Google Patents

Abstract

PURPOSE:To obtain a blue-green alga of the genus Spirulina in a large amount using a readily preparable culture medium, by cultivating the blue-green alga in the culture medium containing a hot water extract of fowl droppings as a nutrient source. CONSTITUTION:A blue-green alga of the genus Spirulina, e.g. Spirulina platensis, Spirulina maxima, Spirulina subsalsa, etc., is cultivated in a readily preparable culture medium containing a hot water extract of fowl droppings as a nutrient source. Sodium hydrogencarbonate is, as necessary, added to the culture medium. The amount of the algal bodies which can be obtained in the culture medium containing both the hot water extract of fowl droppings and the sodium hydrogencarbonate is equal to or more than that obtained in a conventional complicated synthetic culture medium.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a novel method for culturing blue-green algae of the genus Spirulina.

[Conventional technology]

Blue-green algae of the genus Spirulina contain large amounts of protein, β-carotene, vitamins, etc. and have a good nutritional balance, so they are used as raw materials for foods, feeds, and the like. Furthermore, it is expected to be used as an oxygen supply source, food, etc. for future space stations.
Research is being conducted for this purpose.

Conventionally, blue-green algae of the genus Spirulina are
As described in 1999, Imori Publishing, p. 299, it was cultured using a complex 811 synthetic medium containing a large number of inorganic salts. However, since such a medium contains many components, its preparation is complicated and time-consuming;
Cost of culture medium J) It was relatively high. Therefore, it has been desired to develop a medium suitable for industrially cultivating large amounts of Spirulina blue-green algae and a culture method using the medium.

[Problems to be Solved by the Invention] The present invention aims to provide a method for culturing Spirulina cyanobacteria using a medium that can be prepared by a simple method using inexpensive raw materials.

[Means for solving problems]

The above problem can be solved by the method of the present invention, which is characterized in that Spirulina sp. resolved.

The chicken manure used in this invention may be raw chicken manure obtained directly from a poultry farm, or may be dried chicken manure obtained by drying it by heating or the like. When using raw chicken manure, it is preferable to loosen it in a QQ shape in an extraction medium during extraction, and in the case of dried chicken manure, it is preferable to crush it once before the extraction operation.

Preference is given to using hot water as extraction medium.

The ratio of chicken manure and extraction medium in the extraction operation is not particularly limited, but when dry chicken manure is used, it is preferably 0.5 g to 300 g of dry chicken manure to the extraction medium 11,
When using raw chicken manure, it is preferable to use an amount corresponding to the above dry weight for the extraction medium 11. If the concentration of chicken manure at the time of extraction is too low, a large amount of extract liquid must be processed, large extraction equipment is required, and a large amount of heat is required, which is uneconomical. Furthermore, if the concentration of chicken manure during extraction is too high, the extraction efficiency will decrease.

The extraction temperature may range from 70°C to 130°C, and 90°C
A range of from 100°C to 100°C is preferred, and a temperature at or near boiling temperature is most preferred. The extraction time varies depending on the extraction temperature, but is preferably 1 to 5 hours, particularly preferably 2 to 3 hours. If you want to shorten the extraction time, increase the extraction temperature to a high temperature, e.g.
The temperature may be 0° C., and this operation can be performed in a pressure-resistant container.

After extraction, the extraction mixture is separated into solid matter and extract liquid by conventional methods such as centrifugation and filtration. The temperature during separation is not particularly limited.

The extract in the extract obtained as described above) can be used as a medium as it is if the agricultural content is appropriate, or it can be used as a medium after adding sodium bicarbonate to it. I can do it.

In addition, if the extract 4% in the above-mentioned extract is high, after diluting this extract with water to an appropriate 4%, or after further adding sodium bicarbonate to this diluted solution, is used as a medium. Furthermore, the above-mentioned extract can be dried by a conventional method such as freeze drying or spray drying and stored as a solid, and if necessary, this dried product can be dissolved in water or sodium bicarbonate can be added to this solution. A culture medium can be prepared by

The amount or concentration of chicken manure extract in the culture medium is conveniently defined by the amount of dried chicken manure from which the extract is derived. When defined in this way, 0 dried chicken manure per 14 medium
．． Culture medium containing extract derived from 1 to Log, preferably 2 to 4 g, is 3jl F. When adding sodium bicarbonate to the culture medium, the amount added may be appropriately selected within the range of about 30 g/β or less, and about 20 g/j! ~10g/
It is preferable to add in a range of 1. The pH in the medium is 7~
12, preferably in the range of 8.5 to 10.5. The medium prepared as described above usually has a pH within this range, but if the pH is too low it can be adjusted to an appropriate level, for example by adding sodium bicarbonate, and if the pH is too high it can be adjusted to an appropriate pH by passing carbon dioxide. Can be adjusted to the range.

Examples of Spirulina algae that can be cultured by the method of the present invention include Spirulina platensis (Sp
irulina platensis), Spirulina maxima, Spirulina subsalsa (Spirulina 5ubs)
alsa), etc. The culture temperature generally ranges from 20°C to 50°C, preferably from 25°C to 45°C. The optimal culture temperature varies depending on the type of Spirulina, for example, approximately 30°C for Spirulina platensis.
For Spirulina Subsalsa, approximately 40°C is optimal. In culturing, it is necessary to irradiate light, and it is preferable to use natural light (sunlight) as this light, but it is also possible to use an artificial light source such as, for example, a fluorescent lamp. The intensity of light irradiation varies from lXIO3 to lXIO3 on the culture solution surface.
Lux is preferably 7X10' to 1.5X10
It is more preferable to use 'lux'.

When the intensity of light irradiation is high, it is desirable to minimize the death of Spirulina by thoroughly stirring (↑).When irradiating light using an artificial light source, the irradiation time should be set for 10 to 14 hours a day, so that the natural Intermittent irradiation may be performed with a cycle close to that of light, or continuous irradiation may be performed.

Next, the method of the present invention will be explained in more detail with reference to Examples.

200 ml of distilled water was added to 30 g of commercially available dried chicken manure, boiled for 3 hours, and filtered through Toyo Filter Giran 5A to obtain an extract containing a chicken manure extract.

Next, a medium having the following composition was prepared using this extract.

(Medium C is only distilled water) Furthermore, for comparison, a known synthetic medium with the following composition (
SOT medium) was prepared.

Na1lCO31,680m g KzllPOn 50m gNa
NO:+250m gK2SO
t 100m gNaC1210
0mg MgSO4-782020mg CaC(!z ・211.0
4m gFeSO4・7820
1m gNazlEDTA・2tlzO8
m g8, Friday night+＊'
Q, l m (!1 distilled water 9
9.9mJ (*) A S metal mixture HffB (h 286m gMn
SO4・IHzo 250m gZnS
Oa ・7Hz0 22.2m gCII
SO4-5Hz8 7.9m gNaJo
042.1 mg distilled water 100rr+7! 300 m 7 of the above four types of culture media! Each was placed in a 1,000 m2 L-shaped culture tube, inoculated with 0.06 g of Spirulina sublarsa seed algae, and cultured for 12 days at 37° C. under artificial lighting of 17,000 lux. The algal bodies were collected from the culture solution by filtration, dried in a vacuum dryer at 56°C for 24 hours, and the weight of the dried algal bodies was measured.The results were as follows.

A 0.69 B O,25 C0,06 As described above, Spirulina algae can be cultured using the medium of the present invention, which can be prepared very simply, and in particular, the medium containing both chicken manure extract and sodium bicarbonate. When using the culture medium of the present invention, it is possible to obtain the same amount of algae as or more than when culturing in a conventional complex synthetic medium.

Claims (1)

[Claims] 1. A method for culturing blue-green algae of the genus Spirulina, which comprises culturing the blue-green algae of the genus Spirulina in a medium containing a hot water extract of chicken manure as a nutrient source and optionally further containing sodium bicarbonate.