JPS6270755A - Method for examining concentration of solvent for liquid chromatography - Google Patents

Method for examining concentration of solvent for liquid chromatography

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Publication number
JPS6270755A
JPS6270755A JP21176085A JP21176085A JPS6270755A JP S6270755 A JPS6270755 A JP S6270755A JP 21176085 A JP21176085 A JP 21176085A JP 21176085 A JP21176085 A JP 21176085A JP S6270755 A JPS6270755 A JP S6270755A
Authority
JP
Japan
Prior art keywords
solvent
concentration
examined
liquid chromatography
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP21176085A
Other languages
Japanese (ja)
Other versions
JPH0715461B2 (en
Inventor
Atsuji Kitazawa
北澤 厚治
Tetsuya Kawazoe
川添 鉄也
Nobuhiro Oda
信博 織田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kurita Water Industries Ltd
Original Assignee
Kurita Water Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kurita Water Industries Ltd filed Critical Kurita Water Industries Ltd
Priority to JP21176085A priority Critical patent/JPH0715461B2/en
Publication of JPS6270755A publication Critical patent/JPS6270755A/en
Publication of JPH0715461B2 publication Critical patent/JPH0715461B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

PURPOSE:To accurately calculate the concn. of a solvent to be examined, by calculating a calibration curve of solvent concn. and a holding time with respect to a solvent having the same component as the solvent to be examined but known concn. different from that of said solvent to be examined and comparing the same with the holding time of the solvent to be examined. CONSTITUTION:For example, a water-methanol mixture is used as a solvent to be examined. At first, solvents of which the methanol concns. are different over several stages are prepared and used as the mobile phase of liquid chromatography and a sample is injected therein to measure the hold times of said solvents. Then, the calibration curves between solvent concns. and teh holding times are calculated. Further, the sample is injected in the water- methanol solvent mixture being the solvent to be examined and the holding time of said solvent is measured by the liquid chromatography and the holding time of the solvent to be examined is compared with the calibration curves to calculate concn. Because the concn. is calculated from the holding time of the solvent to be examined by the calibration curve of each solvent having known concn., the concn. can be rapidly calculated with high accuracy.

Description

【発明の詳細な説明】 本発明は液体クロマトグラフィー用溶媒の濃度検定方法
に係り、特に液体クロマトグラフィーの移動層として用
いられる溶媒の濃度を、正確、迅速かつ容易に検定する
方法に関する。
DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for testing the concentration of a solvent for liquid chromatography, and more particularly to a method for accurately, quickly and easily testing the concentration of a solvent used as a moving layer in liquid chromatography.

[従来の技術] 液体クロマトグラフィー(以下rLCJと略称すること
がある。)は、可溶性物質を分離・精製する目的で用い
られ、製薬、食品、精密化学等の分野において工業的規
模での分離・精製に応用されている。LCによる分離・
精製方法は吸着物質を充填したカラムの一端からサンプ
ル、展掴液、溶離液を導入し、目的成分を他の成分又は
不純物から展開分離し、カラム他端からの流出液の一部
を検出しながら目的成分と他の成分又は不純物とを順次
回収するものである。
[Prior Art] Liquid chromatography (hereinafter sometimes abbreviated as rLCJ) is used for the purpose of separating and purifying soluble substances, and is used for separation and purification on an industrial scale in fields such as pharmaceuticals, foods, and fine chemistry. Applied to refining. Separation by LC
The purification method involves introducing the sample, developer, and eluent from one end of a column filled with an adsorbent, developing and separating the target component from other components or impurities, and detecting a portion of the effluent from the other end of the column. At the same time, the target component and other components or impurities are sequentially recovered.

LCにおいて、展開液又は溶離液として、即ちLCの移
動層として用いられる溶媒にはメタノール、エタノール
等のアルコール溶液があるが、LC用溶媒はその溶媒濃
度によりサンプルの保持+I輻11!1    Tin
  +  Iζ −相 コ1−1− 目 ^h直」ケへ
 本 しφ   h ニー 込 番ね − 脳るまでの
時間、は大きな影響を受ける。このため、LCによる分
離・精製において、用いる溶媒の濃度を予め正確に把握
しないで分離・精製を行なうと、サンプルの保持時間に
誤差が生じ、高い回収率で分離舎精製を行なうことがで
きない。
In LC, alcohol solutions such as methanol and ethanol are available as solvents used as developing solutions or eluents, that is, as moving layers in LC, but LC solvents have a tendency to retain the sample +I range 11!1 depending on the solvent concentration.
+ Iζ -phase KO1-1- 目 ^h 正” けへ し φ h knee くんね - The time it takes for the brain to become conceivable is greatly affected. For this reason, in separation and purification by LC, if the concentration of the solvent used is not accurately known in advance, an error will occur in the retention time of the sample, making it impossible to perform separation laboratory purification with a high recovery rate.

従来、一般に溶媒の濃度を検定する方法としては、ガス
クロマトグラフィー法、比重測定、粘度測定あるいはU
V測定による方法、更に水の濃度測定にはカールフィッ
シャー法が提案されている。
Conventionally, methods for verifying the concentration of solvents generally include gas chromatography, specific gravity measurement, viscosity measurement, or U.S.
In addition to the V measurement method, the Karl Fischer method has been proposed for measuring the concentration of water.

[発明が解決しようとする問題点] しかるに、ガスクロマトグラフィー法は、■ カラムの
エージングに、約半日かかるため、分析準備に時間がか
かる。
[Problems to be Solved by the Invention] However, in the gas chromatography method, (1) It takes about half a day to age the column, so it takes time to prepare for analysis.

@ 既知濃度のサンプルで検量線を作る場合、ピーク面
積、あるいはピーク高さを用いるため、サンプルの打込
み量の誤差が精度を左右することとなり、正確性に欠け
る。
@ When creating a calibration curve using samples with known concentrations, the peak area or peak height is used, so the accuracy is affected by the error in the sample loading amount, resulting in a lack of accuracy.

等の欠点がある。また、比重測定、粘度測定あるいはU
 V K11l定による方法は精度面で問題がある。
There are drawbacks such as. In addition, specific gravity measurement, viscosity measurement or U
The method using VK11l has a problem in terms of accuracy.

更にカールフィッシャー法は、水分の絶対量を測定する
方法であるため、サンプルの調整、打込み量により誤差
が生じ易く、精度良くΔ11定することが困難である上
に、分析時間が長いという問題がある。
Furthermore, since the Karl Fischer method is a method for measuring the absolute amount of water, it is prone to errors due to sample preparation and loading amount, making it difficult to determine Δ11 with high accuracy, and the analysis time is long. be.

このように、従来の溶媒濃度の検定方法は、いずれも分
析時間、分析操作、精度等で問題を有するが、LCによ
る工業レベルでの分離・精製においては、特に大量の溶
媒を扱い、しかも溶媒濃度の正確な微調整が要求される
ことから、LC用溶媒の濃度を正確、迅速かつ容易に検
出することができる方法の出現が強く望まれていた。
As described above, all of the conventional solvent concentration verification methods have problems in analysis time, analysis operations, accuracy, etc., but in industrial-level separation and purification using LC, especially large amounts of solvent are handled, and Since precise fine-tuning of the concentration is required, there has been a strong desire for a method that can accurately, quickly, and easily detect the concentration of an LC solvent.

[問題点を解決するための手段] 本発明は上記従来の問題点を解決し、LC用溶媒の濃度
を正確、迅速かつ容易に測定するべくなされたものであ
って、 液体クロマトグラフィー用溶媒の濃度を検定する方法に
おいて、 ■ 被検定溶媒と同一成分の、既知の異なる濃度の溶媒
の各々を移動層とする液体クロマトグラフィーにサンプ
ルを打ち込んで、その保持時間を測定し、これによって
溶媒濃度と保持時間との検量線を求める工程と、 (勉 被検定溶媒を移動層とする液体クロマトグラフィ
ーにサンプルを打ち込んで、その保持時間を測定する工
程と、 を有し、該工程[2]の測定結果と工程[1]の検量線
とから被検定溶媒の濃度を求めることを特徴とする液体
クロマトグラフィー用溶媒の濃度検定方法、を要旨とす
るものである。
[Means for Solving the Problems] The present invention has been made to solve the above-mentioned conventional problems and to accurately, quickly and easily measure the concentration of a solvent for LC. In the method of verifying concentration, ■ A sample is loaded into a liquid chromatography system using a moving phase of each solvent of known different concentration, which has the same components as the test solvent, and its retention time is measured, thereby determining the solvent concentration. The measurement in step [2] includes a step of calculating a calibration curve with the retention time, and a step of injecting the sample into liquid chromatography using the test solvent as a moving phase and measuring the retention time. The gist of the present invention is a method for testing the concentration of a solvent for liquid chromatography, which is characterized in that the concentration of the solvent to be tested is determined from the results and the calibration curve of step [1].

以下に本発明について詳細に説明する。The present invention will be explained in detail below.

まず本発明の[1]の工程について説明する。First, the step [1] of the present invention will be explained.

一般に、LC用溶媒としてはへキサン、ベンゼン、クロ
ロホルム、エーテル、アルコール、水あるいはこれらの
混合溶媒、例えば水−メタノール系混合溶媒が用いられ
るが、[1]の工程においては、まずこのような被検定
溶媒と同一成分の、濃度の異なる溶媒(以下、「標準溶
媒」ということがある。)を複数種、調整する。標準溶
媒は、被電間隔、例えば5重量%おき程度、で多種類作
成するのが好ましい。
Generally, as a solvent for LC, hexane, benzene, chloroform, ether, alcohol, water, or a mixed solvent thereof, such as a water-methanol mixed solvent, is used. Prepare multiple types of solvents (hereinafter sometimes referred to as "standard solvents") that have the same components as the test solvent but have different concentrations. It is preferable to prepare a wide variety of standard solvents at electrification intervals, for example, at intervals of about 5% by weight.

次いで、調整された各標準溶媒を移動層とするLCを作
成する。この場合、カラム形状は特に制限はない、また
、LCの固定相としても特に制限はなく、用いるサンプ
ル、溶媒等に応じて適宜選定されるが、例えばアルミナ
、シリカ、マグネシア、粘土、セルロース、炭素、ポー
ラスポリマ等が挙げられる。
Next, an LC using each of the prepared standard solvents as a moving phase is created. In this case, there are no particular restrictions on the column shape, and there are no particular restrictions on the LC stationary phase, which may be selected appropriately depending on the sample, solvent, etc. used, but examples include alumina, silica, magnesia, clay, cellulose, carbon, etc. , porous polymer, etc.

次に、これらのLCに、それぞれサンプルを打ち込む、
用いるサンプルには特に制限はないが、被検定溶媒との
相性で保持時間を正確に測定することが可能なもの、即
ち、LC流出液中のサンプルのピークが鋭角的で鮮明に
現れるものを用いるのが好ましい。
Next, input the samples into each of these LCs.
There are no particular restrictions on the sample to be used, but one should be one that is compatible with the test solvent and allows for accurate measurement of retention time, that is, one in which the peak of the sample in the LC effluent appears sharp and clear. is preferable.

そして、測定された保持時間と標準溶媒の濃度とから、
溶媒濃度と保持時間の2軸からなる座標に検量線を作成
する。
Then, from the measured retention time and the concentration of the standard solvent,
A calibration curve is created on coordinates consisting of two axes: solvent concentration and retention time.

次に工程■について説明する。Next, step (2) will be explained.

するLCを作成する。この場合、LCの型式や固定相の
種類、大きさは工程■で作成したLCと同一のものとす
る0次いでこのLCに工程■で用いたものと同一のサン
プルを打ち込んで、その保持時間を測定する。
Create an LC. In this case, the model of LC, type of stationary phase, and size should be the same as the LC prepared in step ①.Next, load the same sample used in step ① into this LC, and hold it for a long time. Measure.

そしてこの保持時間の測定値と工程■で得られた検量線
とを対照することにより、被検定溶媒の濃度を求めるこ
とができる。
By comparing the measured value of this retention time with the calibration curve obtained in step (2), the concentration of the test solvent can be determined.

[作用] 一般に、LCにおいて、移動層の溶媒濃度のわずかな変
化は、サンプルの保持時間に顕著な影響をチえる。即ち
、LCにおけるサンプルと固定相との結合は、殆ど疎水
性相互作用によるため、サンプルの疎水度が高い程、サ
ンプルは固定相と強く結合し、保持時間は長くなり、ま
た、移動層の疎水度が高い程、即ち、LC用溶媒の疎水
性成分の濃度が高い程、サンプルは溶媒により流出され
易くなり、保持時間は短くなる。
[Effect] Generally, in LC, a slight change in the solvent concentration of the moving phase has a significant effect on the retention time of the sample. In other words, the binding between the sample and the stationary phase in LC is mostly due to hydrophobic interactions. The higher the concentration, that is, the higher the concentration of the hydrophobic component of the LC solvent, the easier the sample will be washed out by the solvent, and the retention time will be shorter.

例えば、固定相としてC6を充填したカラムを用い、水
−メタノール系溶媒の移動層で、ビフェニルをサンプル
としてカラムに負荷した場合、溶媒のメタノールの濃度
が高いと、ビフェニルの保1、シ蒔l7JJは短くなり
、メタノールの濃度が低いと、ビフェニルの保持時間は
長くなる。
For example, when using a column packed with C6 as the stationary phase and loading biphenyl as a sample onto the column with a mobile phase of water-methanol solvent, if the concentration of methanol in the solvent is high, the retention of biphenyl will decrease. becomes shorter, and at lower methanol concentrations, the retention time of biphenyl becomes longer.

本発明は、このように溶媒の濃度に応じてサンプルの保
持時間が変動する現象を利用して、被検定溶媒に適した
LC及びサンプルを選定してLC分析を行ない、サンプ
ルの保持時間と溶媒の濃度との間で検量線を作成し、こ
の検量線から被検定溶媒により測定された保持時間に対
応する濃度を求めるものであり、被検定溶媒の濃度を極
めて容易に知ることができる。
The present invention makes use of the phenomenon that the retention time of a sample varies depending on the concentration of the solvent, selects an LC and sample suitable for the solvent to be tested, performs LC analysis, and calculates the retention time of the sample and the solvent. A calibration curve is created between the concentration of the test solvent and the concentration corresponding to the retention time measured by the test solvent is determined from this calibration curve, and the concentration of the test solvent can be determined very easily.

[実施例] 以下、実施例について説明する。[Example] Examples will be described below.

実施例1 水−メタノール系2液混合溶媒の濃度の検定実験を行な
った。
Example 1 An experiment was conducted to verify the concentration of a two-part water-methanol mixed solvent.

なお、液体クロマトグラフィー装置としては、−島津製
作所製LC4A (オートサンプラー付)分析カラム(
6mmφ、長さ50mm)を用い、固定相としてCa充
填材を充填した。さらに、恒温槽として東京理化器械■
UA−100を用いた。また、標準溶媒として、メタノ
ール濃度が67.5重量%、70重量%、72.5重量
%のメタノール水溶液をiffし、サンプルとしてはウ
ラシルとビフェニルをメタノールに溶解したものを用い
た。
The liquid chromatography device used is - Shimadzu LC4A (with autosampler) analytical column (
6 mm in diameter and 50 mm in length) and filled with Ca filler as a stationary phase. In addition, Tokyo Rika Kikai ■ can be used as a constant temperature bath.
UA-100 was used. Furthermore, methanol aqueous solutions with methanol concentrations of 67.5% by weight, 70% by weight, and 72.5% by weight were used as standard solvents, and samples in which uracil and biphenyl were dissolved in methanol were used.

〈検量線の作成〉 まず、35℃の一定温度のもとに、カラムに67.5重
量%メタノール水溶液をパージした後、この水溶液を移
動層としてサンプルを打ち込み、展開し、サンプルの保
持時間を求めた。この場合、ウラシルはC8に保持され
ないので、ウラシルの溶出時11Dをto、ビフェニル
の溶出時間をt、3とすると、ビフェニルの保持時間t
Rはt R= t B  t U で求められる。
<Creating a calibration curve> First, at a constant temperature of 35°C, after purging the column with a 67.5% methanol aqueous solution, the sample was injected and developed using this aqueous solution as the moving phase, and the retention time of the sample was determined. I asked for it. In this case, uracil is not retained at C8, so if uracil elution time 11D is to and biphenyl elution time t and 3, then biphenyl retention time t
R is determined by t R = t B t U .

同様の操作を70重贋%メタノール水溶液及び72.5
重量%メタノール水溶液についても行な1、%    
 L  h M  3− t(F  Iv  七J  
1+Z  l−/’  −1−Il、  s−ノロ シ
紅吐  口1taを求めた。
Similar operation was carried out with 70% methanol aqueous solution and 72.5%
It was also carried out for a methanol aqueous solution of 1% by weight.
L h M 3-t (F Iv 7J
1+Zl-/'-1-Il, s-sorghum 1ta was determined.

測定されたtRとメタノール濃度との関係をプロットし
て第1図のような検量線を得た。
The relationship between the measured tR and methanol concentration was plotted to obtain a calibration curve as shown in FIG.

〈被検出溶媒の濃度検定〉 高純度メタノール及び水から濃度69.4重量%のメタ
ノール水溶液を作成した。
<Concentration test of detected solvent> A methanol aqueous solution with a concentration of 69.4% by weight was prepared from high purity methanol and water.

検量線作成時の温度と同一のもとでこの水溶液を移動層
として用いて、同様にLCカラムを作成し、サンプルを
打ち込んで展開し、ビフェニルの保持時間tRを求めた
ところ、tll=1.8分であった。
Using this aqueous solution as a mobile phase at the same temperature as when creating the calibration curve, an LC column was created in the same way, a sample was loaded and developed, and the retention time tR of biphenyl was determined; tll = 1. It was 8 minutes.

第1図の検量線により、保持時間1.8分に相当する溶
媒濃度を補間したところ、交点からメタノール濃度は6
9 、4 i量%であることが求められた。
Using the calibration curve in Figure 1, we interpolated the solvent concentration corresponding to a retention time of 1.8 minutes, and found that the methanol concentration was 6 from the intersection point.
It was determined that the content was 9.4%.

この結果、本発明の方法によれば、被検定溶媒の濃度を
正確、迅速かつ容易に求められることが明らかである。
As a result, it is clear that according to the method of the present invention, the concentration of the test solvent can be determined accurately, quickly, and easily.

[発明の効果] 以上詳述した通り、未発明のLC…浣b9の9F!!麻
検定方法によれば、LC用溶媒の濃度を正確、迅速かつ
容易に求めることができる。
[Effect of the invention] As detailed above, the uninvented LC...9F of Kasuri b9! ! According to the hemp assay method, the concentration of the LC solvent can be determined accurately, quickly, and easily.

このため、本発明方法を採用することにより、測定時間
の短縮が図れ、またサンプルの打ち込み等に高度な技術
や厳密性を要しないため、測定操作の簡易化を図ること
ができる。更に、ガスクロマトグラフィー装置等のLC
装置以外の装置を必要としないことから、設備費等の軽
減が可能となりコスト面でも極めて有利である。
Therefore, by employing the method of the present invention, the measurement time can be shortened, and the measurement operation can be simplified because high technique and precision are not required for sample implantation, etc. Furthermore, LC such as gas chromatography equipment
Since no equipment other than the device is required, it is possible to reduce equipment costs and is extremely advantageous in terms of cost.

【図面の簡単な説明】[Brief explanation of drawings]

第1図は実施例1で得られた検量線を示すグラフである
。 代 理 人   弁理士   重 野  剛第1図 メタノール濃度(!重%) 手続補正書 昭和60年11月25日 特許庁長官殿              Vバ1、l
 415件の表示 昭和60年#許m第211760号 2 発IJJの名称 液体クロマトグラフィー用溶媒の濃度検定方法3 補正
をする者 4¥件との関係  特許出願人 名 称    (106)栗田工業株式会社4 代理人 住 所  東京都港区赤坂4丁目8番19号〒107 
 赤坂表町ビル502号 6 補正の対象 明細書の特許請求の範囲の欄及び発明の詳細な説明の欄
。 7 補正の内容 (1)  特許請求の範囲を別紙のとおりに訂正する。 (2) 明細書第2頁第3行及び第17行、同第4頁最
ド行、同第5頁第4行、同第6頁第3行及び最下行、同
第7頁第10行、第15行及び最下行、同第9頁第11
行並びに同第10頁第8行に「移動層ノとあるのを、そ
れぞれr移動相、1と訂正する。 以  上 別紙 特許請求の範囲 (])  液体クロマトグラフィー用溶媒の濃度を検定
する方法において、 ■ 被検定溶媒と同一成分の、既知の異なる濃度の溶媒
の各々をl&Jとする液体クロマトグラフィーにサンプ
ルを打ち込んで、その保持時間を測定し、これによって
溶媒濃度と保持時間との検量線を求める工程と、 ■ 被検定溶媒をW肱」とする液体クロマトグラフィー
にサンプルを打ち込んで、その保持時間を測定する工程
と、 を有し、該工程[2]の測定結果と工程[1]の検量線
とから被検定溶媒の濃度を求めることを特徴とする液体
クロマトグラフィー溶媒奴の濃度検定方法。
FIG. 1 is a graph showing the calibration curve obtained in Example 1. Agent Patent Attorney Tsuyoshi Shigeno Figure 1 Methanol concentration (!% by weight) Procedural amendment November 25, 1985 To the Commissioner of the Japan Patent Office Vba1, l
Display of 415 items 1985 #Permit No. 211760 2 Name of IJJ Concentration verification method of solvent for liquid chromatography 3 Person making amendment 4 Relationship with item Patent applicant name Name (106) Kurita Water Industries, Ltd. 4 Agent address: 4-8-19 Akasaka, Minato-ku, Tokyo 107
Akasaka Omotemachi Building No. 502 No. 6 Claims column and Detailed Description of the Invention column of the specification subject to amendment. 7. Contents of amendment (1) The scope of claims is corrected as shown in the attached sheet. (2) Lines 3 and 17 of page 2 of the specification, line 4 of page 4, line 4 of page 5, line 3 and bottom of page 6 of the specification, line 10 of page 7 of the specification , line 15 and bottom line, page 9, number 11
line and page 10, line 8 of the same page, "mobile phase" is corrected to read "r mobile phase" and "1, respectively." The above attached claims (]) Method for assaying the concentration of a solvent for liquid chromatography In this case, ■ Inject the sample into a liquid chromatography system using L&J as each solvent with the same component as the test solvent and at a known different concentration, measure the retention time, and use this to create a calibration curve between solvent concentration and retention time. (1) Injecting the sample into a liquid chromatography system using the solvent to be tested and measuring its retention time; A method for testing the concentration of a liquid chromatography solvent, characterized by determining the concentration of the solvent to be tested from a calibration curve.

Claims (1)

【特許請求の範囲】[Claims] (1)液体クロマトグラフィー用溶媒の濃度を検定する
方法において、 [1]被検定溶媒と同一成分の、既知の異なる濃度の溶
媒の各々を移動層とする液体クロマトグラフィーにサン
プルを打ち込んで、その保持時間を測定し、これによっ
て溶媒濃度と保持時間との検量線を求める工程と、 [2]被検定溶媒を移動層とする液体クロマトグラフィ
ーにサンプルを打ち込んで、その保持時間を測定する工
程と、 を有し、該工程[2]の測定結果と工程[1]の検量線
とから被検定溶媒の濃度を求めることを特徴とする液体
クロマトグラフィー用溶媒の濃度検定方法。
(1) In a method for verifying the concentration of a liquid chromatography solvent, [1] A sample is introduced into a liquid chromatography system in which each of the solvents having the same components as the test solvent and having different known concentrations is used as a moving phase. A step of measuring the retention time and thereby obtaining a calibration curve between solvent concentration and retention time; [2] A step of injecting the sample into liquid chromatography using the test solvent as a moving phase and measuring the retention time. , A method for testing the concentration of a solvent for liquid chromatography, characterized in that the concentration of the solvent to be tested is determined from the measurement results of step [2] and the calibration curve of step [1].
JP21176085A 1985-09-25 1985-09-25 Liquid Chromatography Solvent Concentration Assay Method Expired - Lifetime JPH0715461B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP21176085A JPH0715461B2 (en) 1985-09-25 1985-09-25 Liquid Chromatography Solvent Concentration Assay Method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP21176085A JPH0715461B2 (en) 1985-09-25 1985-09-25 Liquid Chromatography Solvent Concentration Assay Method

Publications (2)

Publication Number Publication Date
JPS6270755A true JPS6270755A (en) 1987-04-01
JPH0715461B2 JPH0715461B2 (en) 1995-02-22

Family

ID=16611122

Family Applications (1)

Application Number Title Priority Date Filing Date
JP21176085A Expired - Lifetime JPH0715461B2 (en) 1985-09-25 1985-09-25 Liquid Chromatography Solvent Concentration Assay Method

Country Status (1)

Country Link
JP (1) JPH0715461B2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH067708U (en) * 1992-06-30 1994-02-01 トノクラ医科工業株式会社 Cardiac / thoracic ratio measuring device
JP2008292378A (en) * 2007-05-25 2008-12-04 Yamazen Corp Anti-phase liquid chromatograph control device, anti-phase liquid chromatography execution method, and anti-phase liquid chromatograph control program
JP2010153276A (en) * 2008-12-26 2010-07-08 Hitachi Ltd Concentration detecting system for fuel cell and fuel cell power generation system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH067708U (en) * 1992-06-30 1994-02-01 トノクラ医科工業株式会社 Cardiac / thoracic ratio measuring device
JP2008292378A (en) * 2007-05-25 2008-12-04 Yamazen Corp Anti-phase liquid chromatograph control device, anti-phase liquid chromatography execution method, and anti-phase liquid chromatograph control program
JP2010153276A (en) * 2008-12-26 2010-07-08 Hitachi Ltd Concentration detecting system for fuel cell and fuel cell power generation system

Also Published As

Publication number Publication date
JPH0715461B2 (en) 1995-02-22

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