JPS6262243A - Automatic blood specimen preparing apparatus - Google Patents

Abstract

PURPOSE:To automatically prepare a specimen from blood sampled and store it into a cassette, by housing a slide glass into a dyeing cage as centrifugally smeared with blood after dripped to prepare a specimen by dyeing and stored it into a cassette. CONSTITUTION:A blood parcelling/shifting section moves on a mobile table and blood is parcelled from a test tube of a test rack 11 to be dripped onto a slide glass 10 placed at a centrifugally smearing section 4. As a rotor rotates, the slide glass 10 centrifugally smeared with blood is stored into a specimen stacker; after being dried,it is shifted to a dyeing cage 51, which is passed through liquid tanks 70, 71 and 72, and then the specimen is drained 108, dried and dyed. After its dyeing, the specimen slide glass 10 is stored 7 into a cassette.

Description

[Detailed description of the invention]

<Field of Industrial Application> The present invention relates to an automatic blood specimen preparation device that automatically prepares blood specimens. <Summary of the Invention> The automatic blood specimen preparation device according to the present invention includes a slide glass supply unit that supplies a slide glass, and a centrifugal smearing unit that drips blood onto a slide glass supplied from the slide glass supply unit and centrifugally smears it. a staining section that creates a specimen by staining the slide glass smeared with blood by this centrifugal smearing section while stored in a staining basket, and a cassette storage section that stores the specimen created by this staining section in a cassette. This system automatically creates specimens of collected blood and stores them in all cassettes. Conventional technology> Preparing a specimen of collected blood

[2] The work of storing this in a cassette has conventionally been done entirely manually, as there is no device that can do this automatically. <Problems to be Solved by the Invention> For this reason, in the past, it required a great deal of effort to prepare specimens, and the specimens were often touched after being smeared with white liquid, which was not desirable from the standpoint of hygiene management. In addition, since the specimens are prepared manually, when storing the specimens in cassettes, there are times when the front and back of the specimens are mistaken, or errors occur in the order of the specimens. SUMMARY OF THE INVENTION In view of the above circumstances, an object of the present invention is to provide an automatic blood sample preparation device that can automatically prepare a sample from collected blood and store the sample in a cassette. Problem A, ft-Means for Solving> In order to solve the above problems, the automatic blood sample preparation device according to the present invention includes a slide glass supply unit that supplies slide glasses, and a slide glass supply unit that supplies slide glasses. a centrifugal smearing section that centrifugally smears blood dropped onto a slide glass; a staining section that stains the slide glass smeared with blood by the centrifugal smearing section while it is housed in a staining basket to prepare a specimen; The present invention is characterized in that it includes a cassette storage section that stores the specimen made by the staining section in a cassette. <Embodiment> FIG. 1 is a perspective view showing an embodiment of an automatic blood specimen preparation apparatus according to the present invention, and FIG. 2 is a plan view of the same embodiment. The automatic blood specimen preparation device shown in these figures automatically collects the blood in these test tubes and slides it by placing a test tube rack 1 containing a large number of test tubes containing collected blood into the device. A drop is placed on a glass, and then it is smeared, stained, dried, and stored in a cassette.As shown in Fig. 1, there is a slide glass supply section 1, a slide glass conveyance section 2, and a blood separation and transfer section. 3, a specimen transfer section 30,
The apparatus includes a centrifugal smearing section 4, a rotary conveyance section 5, a staining section 6, a cassette storage section 7, an operation section 8, and a display section 9. As shown in the main part side view m of FIG. The slide glass 10 housed in the slide glass case 20 is equipped with an extrusion unit 21 that pushes out the sample slide glasses 10 one by one from the bottom of the case. The slides are pushed out one by one to position a, and are fixed to one edge (this edge is bent downward) of the abbreviated moving table 35 of the slide glass transport section. A shaft 22 located above, a pair of suction pads 23 provided at the lower end of this shaft 22, and a tube 25 that communicates these suction pads 23 with a small pump (not shown) built in the device main body 27. It is comprised of an elevating pulley 24 that raises and lowers the shaft 22, and an L-shaped angle 13 that fixes the elevating pulley 24 to the edge of the moving table 35. When a drive unit (not shown) built in the device body 27 is turned on, the box body 26 is moved to the rails by a driving force transmission mechanism (not shown) located between the device body 27 and the box body 26. 2
8 (direction of arrow C)). In this case, the box body 26 is smoothly moved by the movable roller 12 at the lower end of the movable table 35 whose part (vertical portion) is fixed to the box body 26, and accordingly The attached slide glass transport section 2 and specimen transport section 30. The blood separation and transfer section 3 moves together with this. The blood fraction transfer unit 3 moves freely in the directions of arrows 1 and 3 in the shaft body 26 to separate all the blood from the test tubes in the test tube rack 11. The blood is dropped onto a slide glass 10 placed in a centrifugal smearing section 4, which will be described later.
7, a moving block to which this nozzle lifting motor 14 and the lifting pulley 37 are attached] 5, and this moving block 15 are indicated by an arrow ←
the wire 16 to be moved in the direction, and the moving block 15
and a drive mechanism (not shown) for moving in the direction of arrow C). The liquid dispersion transfer section 3 moves the movable block 15 within the range of the movable table 35 as indicated by the arrow (A), no matter where the movable table 35 is located.
The nozzle 36 is washed by moving the nozzle 36 up and down while moving the blood in the test tube in the centrifugal smear section 4 and dripping it onto the slide glass 10. The specimen transfer section 30 is for transporting a specimen slide glass (specimen) created in the centrifugal smearing section 4, which will be described later, to the next process (stock section), and includes a shaft 31 and a lower end of this shaft 31. one suction pad 32 provided in the main body 27, and a tube 3 that communicates this suction pad 32 with a small pump (shown in the diagram) built into the main body 27 of the device.
4, a lifting pulley 33 for lifting and lowering the shaft 31;
A shaft lifting motor 17 that drives this lifting pulley 33
, this shaft elevating motor 17 and the elevating pulley 33
and an angle 18 for fixing the movable table 35 to the other edge of the movable table 35. Since this specimen transfer section 30 is fixed to the moving table 35, the slide glass transfer section 2 can move the slide glass 1
When transporting the specimen 0, it moves together with the centrifugal smearing section 4 to transport the specimen made in the centrifugal smearing section 4 to the next step. The centrifugal smear unit 4 also includes a motor, a plurality of guide bins 42, and a lid 44 that closes the top of the rotor 40, as shown in FIG.
It is composed of: The slide glass supply section 11, the slide glass transport section 2, the blood separation and transfer section 3, the specimen transfer section 30, and the centrifugal smear section 4 operate as described below. First, the slide glass lO pushed out to position a is moved between a pair of suction pads 2 provided on the slide glass transport section 2.
3, its both ends are attracted and transferred in the direction of arrow (A). Then, it is set inside a guide bin 42 provided on the rotor 40 of the centrifugal smearing section 4. Next, the nozzle 36 is moved along the moving table 35 to the test tube rack 11, where it is lowered to disperse the blood in the test tubes, and then moved along the moving table 35 to the centrifugal smearing section. The blood is transported to 40 locations and dripped onto the surface of the slide glass 10 set on the rotor 40. Thereafter, the nozzle 36 opens the cleaning tank 43 (second
(see figure) and is cleaned, and blood adhering to this nozzle 36 is removed. On the other hand, when blood is dropped onto the surface of the slide glass 10, the lid 44 of the rotor 40 is closed, and then the rotor 40 is rotated to centrifugally smear the blood dropped onto the slide glass 10. Then, the specimen slide glass created by centrifugally smearing the blood dropped onto the slide glass IO is attached to its left end portion (see FIG.
In B), the left end) is suctioned and transferred to a specimen stacker (stock section) 45 shown in FIG. This operation is repeated until the specimen stacker 45 is stocked with a predetermined number of slide glasses. In this case, as shown in FIG. 2, the specimen stacker 45 is provided with a large number of grooves on opposing surfaces of both side walls, and the glass insertion side is formed wide so that the specimen slide glass can be easily inserted. . The specimen slide glasses stocked in the specimen stacker 45 are dried in a drying tube 46 provided at the top of the specimen stacker 45, and then pushed out by the shaft 31 as shown in FIG. 5
supplied to The rotary transport unit 5 includes a rotating plate 50, a staining basket 51 attached to the rotating plate 50, and a suppressing unit 52 that pushes out a specimen slide glass that has undergone a staining process, which will be described later, from the staining basket 51 to the cassette side. It is configured. In this case, the dyeing basket 51 has side plates 5 as shown in FIG.
1a, four pins 51b provided on the outer surface of the upper end side of these both side plates 51a, a groove 5]c provided at the center of the outer surface of the both side plates 51m, and four long plate-shaped pins 5]c provided between the both side plates 5]2L. It is configured to include a plurality of guide rods 51d provided on the angle 51e and two rods 51f for preventing the sample slide glass placed between the grooves 51o from falling. Further, the rotary plate 50 has a pair of guide blocks 54 having two guide rollers 53 that engage with the @51c.
and a pair of stoppers 55 corresponding to these guide blocks 54, by which the dyeing basket 51 is held on the rotary plate 50. Furthermore, as shown in FIG. On the back side of the rotating plate 50,
Large gears 56 are scattered around, and small gears 57 are fixed to the shaft of the motor (not shown) when the motor (not shown) is driven.
The rotary plate 50 is urged to rotate by the large front gear 56 that meshes with the small gear 57. Further, the pressing portion 52 is connected to the motor 58 of the motor 58.
A drive boo IJ59 attached to the drive pulley 59, a passive pulley 100 provided corresponding to the drive pulley 59, and a belt 60 hung between these pulleys 59 and 100.
A slide bearing 61 attached to this belt 60,
A slide shaft 63 into which this slide bearing 61 is inserted, a support bracket 62 that fixes both ends of this slide shaft 63 to the main body side, an extrusion block 64 that is fixed to the slide bearing 61, and the extrusion block 64 that is driven by the motor 58. The push-out block 64 is provided with two sensors 65 and 66 for detecting the position of the push-out block 64 when the push-out block 64 is driven. The rotary conveyance unit 5 configured in this manner operates as described below. First, when a certain number of sample slide glasses are stocked in the sample stacker 45, the shaft 31 pushes out the sample slide glasses and transfers them to the staining basket 51. Next, the rotary plate 50 rotates by 90 degrees and stops, and at this position the dyeing basket 51 is transferred to the dyeing section 6, which will be described later. When the dyeing process is finished in the dyeing section 6 and the dyeing basket 51 is returned to this position, the rotary plate 50 further rotates by 90 degrees and stops. Next, the motor 58 of the pressing unit 52 operates the push-out block 64 in the arrow e→force direction to push out the specimen slide glass from the staining basket 51 and transfer it to the cassette. Thereafter, the suppressing portion 52 returns to its original state, and the rotary plate 50 rotates 180 degrees in the opposite direction to return the dyeing basket 51 to its initial position. The staining section 6 also includes a liquid tank 70 containing a light staining solution and a liquid tank 71 containing a fixing dye solution, as shown in FIG.
, a liquid tank 72 containing water, a draining table 108, a stenna section 73 that sequentially transfers the dyeing basket 51 to each of the liquid tanks 70 to 72 and the draining table 108 for immersion, swinging, and draining; When the dyeing basket 51 is transferred to the draining table 108 and dried by the section 73, the dyeing basket 51 is provided with a drying cylinder 101 for quickly drying the dyeing basket 51. In this case, as shown in FIG. 6(A), an arm 75 is identified on the upper part of the stenna shaft 74 constituting a part 73 of the stena 12, and this arm 75 has the bin 51b of the staining basket 51. 'e A hook plate 76 is attached. Further, as shown in FIGS. 6(A) and 6(B), a slide bearing 77 is fixed to the lower part of the stena shaft 74, and a swing mechanism 78 is attached to the slide bearing 77.
The stenna shaft 74 is driven horizontally and vertically. The swing mechanism 78 includes two base plates 79, a motor 80 fixed to these base plates 79, and a motor 80.
an eccentric lever 81 fixed to the shaft of the eccentric lever 81; a lever 82 having one end rotatably fixed to the base plate 79; the lever 82 rotatably supports the lever 82; a cam floor 83 in contact with the lever 81, and sensors 91, 92, 9 for detecting the vertical position of the lever 82;
3.94. In this case, the sensor 91 detects whether the hook plate 76 has reached its upper limit. In addition, the sensors 92, 9
4 detects the upper and lower limits of the vertical movement of the dyeing basket 51 during the drying process during dyeing. In addition, sensors 93, 9
4 detects the upper and lower limits when the staining basket 51 is immersed in each of the liquid tanks 70 to 72 and is swung up and down, and the specimen slide glass in the staining basket 51 is uniformly distributed by this vertical swinging. dyed. Further, this swing mechanism 78 includes a slide bearing 77 connected to the lever 82 via a pin 84 formed at the tip of the lever 82 and engaging with a nine U-shaped notch;
A slide shaft 85 into which this slide bearing 77 is inserted and a fixing part 95 that fixes both ends of this slide shaft 85 are provided, and when the motor 80 is operated, the slide bearing 7
7 is configured to move up and down the stenna shaft 74. Furthermore, a slide shaft 86 and rollers 87 are formed at the lower part of the pace plate 79, and the base plate 79 is horizontally moved back and forth by a horizontal movement mechanism consisting of a motor 88, a drive pulley 89, and a transmission wire 90. It looks like this. This swing mechanism 78 is connected to the motor 80°88.
is driven to move the staining basket 5 containing specimen slide glasses mounted on the rotary conveyance section 5 into each of the liquid tanks 70 to 70.
72, it is conveyed to the draining table 108 and dyed there. Thereafter, the swing mechanism 78 returns the dyeing basket 51 after dyeing to the rotary conveyance section 5. Furthermore, as shown in FIG. 2, the cassette storage section 7 has a cassette supply line 102 where cassettes before storing specimen slide glasses are lined up, and a storage line 104 where nine cassettes containing specimen slide glasses f are lined up. A biasing unit 103 that biases the cassette on the cassette supply line 102 in the direction of the arrow) and a biasing unit 103 that pushes the cassette containing the specimen slide glass in the direction opposite to the direction of the arrow at the specimen slide glass storage position After pushing out the cassette at the beginning of the cassette row, which is urged by the urging section 103, the cassette at the head of the cassette row is urged in the direction of the arrow (E) and pushed out at the specimen slide glass storage position bt.
15-Y button 106. Further, the operation section 8 includes a numeric keypad and various operation keys, and each section of the apparatus is controlled by operating these various keys. The display section 9 is equipped with a CRT (cathode ray tube) and displays information on each section of the apparatus described above. <Effects of the Invention> As explained above, according to the present invention, a specimen can be automatically created from collected blood and stored in a cassette.

[Brief explanation of the drawing]

FIG. 1 is a perspective view showing one embodiment of an automatic blood sample preparation device according to the present invention, FIG. 2 is a plan view of the same embodiment, and FIG. 3 (A
) is a side view of the main parts of the slide glass supply section, blood separation transfer section, and slide glass transfer section in the same embodiment, and Fig. 3 (
B) is a view taken in the X direction of FIG. 3(A), FIG. 4 is a front view of the rotary conveyance unit in the same embodiment, and FIG. 5 is a perspective view of the dyeing power, color, and rotary plate in the same embodiment. , FIG. 6(A) is a front view of the main part of the dyeing section in the same example, and FIG. 6(B) is a
It is a partial arrow directional view of figure (A) in the y direction. ]... Slide glass supply section, 2... Slide glass transport section, 3... Blood separation and transfer section, 4... Centrifugal smear section, 5... Rotating transfer section, 6... Staining section , 7... Cassette storage section, 8... Operation section, 30... Specimen transfer section.

Claims (1)

[Claims] a slide glass supply unit that supplies a slide glass; a centrifugal smear unit that drips blood onto a slide glass supplied from the slide glass supply unit and centrifugally smears it;
A staining section that creates a specimen by staining a slide glass smeared with blood by this centrifugal smearing section while stored in a staining basket, and a cassette storage that stores the specimen made by this staining section in a cassette. An automatic blood specimen preparation device comprising: