JPS6253756A - Rotor for centrifugal separator - Google Patents

Abstract

PURPOSE:To obtain a rotor capable of preventing damage and capable of separating small specimen particles, by providing a through-hole capable of permitting the passage of a liquid only from above to the axis of a rotor core in the up-and-down direction to allow a cooling fluid through said through-hole. CONSTITUTION:A specimen 16 is flowed in the ring shaped cavity in a rotor body from below through a passage 28 in such a state that a rotor is stopped. At this time, the penetration of a liquid into a through hole 24 is prevented by a spherical float 26. The end part of a liquid passing hole 19 is closed and the rotor body 1 is rotated. When arrangement is generated to a centrifugal force direction at about 3,000rpm, a buffer solution innoxious to the specimen 16 is flowed from above. The buffer solution flows down through the through- hole 24 to be discharged while pushing away the spherical float 26. By this method, operation can be performed while a seal or a bearing is cooled. A high-density extruding liquid 30 is injected from below in a rotary state and the specimen in the rotor body 1 is recovered. By fractionating and taking in the discharged liquid in parts, the specimen can be separated.

Description

[Detailed description of the invention] [Field of application of the invention] The present invention relates to a rotor for a centrifuge.

[Background of the invention]

A conventional centrifuge will be explained with reference to FIGS. 4 and 5. Figure 4 is a schematic structural diagram of the entire general drive system, Figure 5 (a) is a sectional view of a conventional centrifugal rotor, and ← is 0).
−■ is a cross-sectional view of the part viewed from the arrow. In Fig. 4, 1 is the rotor body, 2 is the rotor core, 3 is the shaft fixing nut, and 4 is the rotor core.
．． 5 is a hollow upper and lower shaft, 6 is an upper plate, 7 is a CX upper plate, 8 is a bearing, 9 is a drive unit...Using, a sword is a drive unit stator, and 11 is a drive unit rotor. n is a fixed seal, B is a fixed seal holding part,
M is the vibrating line, which is shaped like an arrow so that the sample can go in and out, and is the outer diameter part of the open core. The rotor body 1 is disposed vertically in the axial center direction, is fixed at the top and bottom to an upper shaft 4 and a lower shaft 5, and is rotationally driven by a drive unit connected to the upper shaft 4. .

The ends of both the upper and lower shafts 4.5 are in contact with fixed seals β for loading and unloading the sample.

In FIG. 5, numeral 1 denotes a rotor inner diameter portion, and numeral 18 denotes a cover, which is connected to both end surfaces of the rotor body 1 in the vertical direction via a liquid sealing mechanism. b is a cover liquid flow hole, 21 is a liquid flow path, and 2 is a sector. At the upper and lower ends of the rotor core 2, liquid flow passages 4 are provided which communicate with the stones on the outer diameter of the core, and during centrifugation, one serves as a sample introduction passage and the other serves as a passage for discharging the supernatant after separation. In such a conventional rotor and centrifugal separator, when centrifuging is performed while rotating at high speed, heat is generated in the stationary seal n section and the bearing 8 section. The higher the rotational speed and the lower the sample flow rate, the higher the exothermic temperature becomes. General K
The smaller the particles of the sample to be separated, the higher the rotational speed or the lower the sample flow rate, which may cause the sample to lose its activity or cause damage to the stationary seal 2 or bearing 8 due to the heat generation. . In addition, since the structure of the rotor body 1 is such that the sample enters and passes through a hollow cavity and comes out, if the centrifugation conditions for the sample are not sufficient, particles separated within the rotor body 1 may However, it may be mixed into the supernatant and discharged.

- [Object of the Invention] The present invention was made in view of the above situation, and its purpose is to provide a rotor for a centrifuge bar that can prevent damage to a centrifuge and separate small sample particles. be.

[Summary of the invention]

The rotor holder for a centrifugal separation frame of the present invention has a cylindrical rotor body and a cover liquid flow hole connected to the connecting portions of both vertical end surfaces of the rotor body through a liquid sealing mechanism in the axial center direction, as described above. A cover in which a liquid flow passage is formed between the end faces of the rotor core disposed within the rotor body, and an annular cavity that communicates with the cover liquid flow hole through the liquid flow passage between the inner periphery of the rotor body. In addition, vane-like sectors that divide the annular cavity into a plurality of parts circumferentially are provided protruding from the outer periphery and are fixed to the rotor core built into the rotor body and to the upper and lower covers, respectively, and the sample to be separated is inside the rotor core. The rotor core is provided with hollow upper and lower shafts that are rotated by a drive unit, and a check valve part that is opened in the fine axis direction of the rotor core and that prevents the liquid from flowing upward at the lower end thereof. A through-hole is provided. That is, through holes are provided in the vertical direction of the axial center of the rotor core through which liquid can flow only from above, so that cooling can be performed by allowing cooling fluid to flow through the rotor core.

[Embodiments of the invention]

Hereinafter, the rotor for a centrifugal separation bar according to the present invention will be described as an example, and the same parts as the conventional one will be denoted by the same reference numerals, and the explanation of the structure of the same parts will be omitted.
Figure 1 (A) is a vertical sectional view of the rotor, ←) is a 1-1 arrow sectional view of (A), and Figure 2 is a sectional view of the rotor.
FIG. 2 is a perspective view of the rotor core shown in FIG. In the figure, a through hole 24 is opened in the rotor core 2 in the axial direction, and a check valve part 5 is provided at the lower end side of the large through hole, and a spherical float Z is provided in the check valve part. In the case of upward flow from the liquid, the check valve is formed such that the spherical float 26 comes into contact with the tapered portion of the check valve to prevent the flow of liquid. The spherical float 26 is hollow and is formed to float lighter than the liquid, and is pushed away when liquid flows in from above, allowing the liquid to flow. In addition, at the lower end of the rotor core 2, a plurality of liquid flow passage rows are provided in the lower cover B, which contact the inner peripheral part of the lower cover and communicate with the lower side of the check valve part 6 and the lower cover liquid distribution large blade. There are two core lower flange n formed on the outer periphery in contact with the circumferential surface. An annular cavity is formed on the upper surface of the lower core flange 4 and is partitioned by sectors formed between the inner circumference of the rotor body l and the outer circumference of the rotor core 2.

Figure 3 is an explanatory diagram of the centrifugation process. The liquid is injected from the lower side of the rotor body 1 through the liquid flow passageway leading to the inner diameter part 1 of the rotor and flows into the annular cavity inside the rotor body 1.At this time, the spherical float 3 of the check valve part 5 moves into the check valve tapered part. 29 and prevents liquid from entering the through hole.Furthermore, the air inside the rotor body 1 is discharged from the upper end as shown by arrow E.

After injecting the liquid, the ends of the upper and lower cover liquid distribution blades are closed and the rotor body 1 is driven to rotate. When the rotor body 1 is driven to rotate, the liquid in the rotor body 1, which was arranged in the direction of gravity when stopped, changes direction and is arranged in the direction of centrifugal force, as shown in 6:I)K. This arrangement ends at around 3000 rFm. Thereafter, as shown in (c), a buffer solution or distilled water that is not harmful to the sample 16 is flowed from above the rotor body l as shown by the arrow A. The buffer solution or distilled water flows down through the through hole, displacing the spherical float, and is discharged as shown by arrow B, without affecting the sample 1, low-high pressure density liquid J, and 22 in the annular cavity inside the rotor. In this way, a cooling liquid of buffer solution or distilled water is passed through the fixed seal 2,
In particular, when the bearing 8 etc. are accelerated while being operated while being cooled to a predetermined rotational speed at which sample r can be separated,
A centrifugal force acts on the particles in the sample 1, and the particles move to a low-high density liquid m%n having the same density as their own rmR, and separation is performed. After that, the speed is reduced to 300071) m.

In this 5ooo rpm state, inject the extrusion liquid (material) with a higher density than the high density liquid 4 injected into the rotor body 1 from below as indicated by the arrow OK in 0) to fill the sample inside the rotor body 1. Collect. The extruded liquid (9) is blocked by the check valve part and does not flow into the through hole, but is injected into the outer circumferential side of the annular space in the rotor inner diameter part 1 through the liquid flow passage hole, as shown in (2). Extrusion liquid (capital) is placed on the outer periphery of the annular cavity.
is located, and the liquid in the rotor annular cavity is pushed out and discharged from the upper liquid flow passage in order of weight. The liquid to be separated can be recovered by subjecting this discharged liquid to JIK fractionation.

In this way, the rotor for centrifugal separation bars of this embodiment has a through hole in the axial center of the rotor core that has a check valve formed to prevent flow from below to above, so that buffer solution or distilled water can be Since the sample inside the rotor can be cooled by flowing from the top to the bottom, it is possible to prevent the temperature from rising in various parts such as fixed seals and bearings, prevent damage to the centrifuge, and separate small sample particles. Incidentally, the ability to separate particles of a sample is determined by centrifugal force x time, and it is effective because temperature rise is prevented with respect to high speed rotation and time, respectively. In addition, an annular cavity is formed on the upper surface of the core lower flange so that the liquid entering the annular cavity flows from the outer peripheral side of the annular cavity, so the supernatant is separated within the annular cavity and the particles are mixed with the supernatant and discharged. never be done.

〔Effect of the invention〕

As described above, the rotor for a centrifugal separation frame of the present invention has the effect of preventing damage to the centrifuge and separating small sample particles.

[Brief explanation of the drawing]

FIG. 1 shows an embodiment of the rotor for centrifugal separation bars of the present invention,
(A) is a longitudinal sectional view, ←) is a sectional view taken along the I-I arrow in 0), Figure 2 is a perspective view of the rotor core in Figure 1, Figure 3 is 0), ←)
, e→, ni) is an explanatory diagram of each operation from injection of liquid such as sample into the rotor to discharge after separation in Figure 1, Figure @4 is a schematic structural diagram of the entire drive system of a general centrifuge, Figure 5 shows a conventional rotor for centrifugal separation bars, 0) is a vertical cross-sectional view, ←)
0) is a sectional view taken along the line v-■. 10. rotor body, 2. ,o-! Core, 4, Upper shaft, 50. Lower shaft, 1. . Cover, blade 1. Cover liquid flow large, 21.28. , liquid flow path, phantom, sector, through hole, 5, check valve part, 1
0. Core lower flange.

Claims (1)

[Scope of Claims] 1. A cylindrical rotor body, and a rotor body that is connected to connection portions on both vertical end surfaces of the rotor body through liquid sealing mechanisms and has a cover liquid flow hole in the axial center direction. a cover in which a liquid flow passage is formed between end faces of the rotor core disposed therein, and an annular cavity that is communicated with the cover liquid flow hole through the liquid flow passage between the inner periphery of the rotor body; Further, blade-shaped sectors that divide the annular cavity into a plurality of parts in the same circumferential direction are provided protruding from the outer periphery and are fixed to the rotor core built into the rotor body and the upper and lower covers, respectively, and the sample to be separated is circulated inside. and a hollow upper and lower shaft rotatably driven by a drive unit, the rotor core is provided with a check valve part that is opened in the axial direction at the center of the shaft and is disposed at the lower end to prevent liquid from flowing upward. A rotor for a centrifuge, characterized in that a through hole is provided. 2. The annular cavity has an outer periphery fitted to the inner periphery of the lower cover, and the liquid flow passage communicating with the lower side of the check valve and the lower cover liquid circulation hole is connected to the inner periphery of the lower cover. The rotor for a centrifugal separator according to claim 1, wherein the rotor is formed on the upper surface of the core lower flange formed on the outer circumferential portion thereof in contact with the upper surface of the core lower flange.