JPS62502840A - Apparatus and method for dividing plant seed material - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] For dividing plant seed material Apparatus and method The present invention relates to the improvement of micro-propagation of plants, and in particular to the division of plant seed material. TECHNICAL FIELD The present invention relates to a method and apparatus for.

Technology background The benefits of micropropagation are well known.

(i) It is convenient for disease control with consequent improvement in plant quality. Provide an effective method. Being free of disease is an imposition in promising export markets such as the Middle East. has become an increasingly important characteristic in relation to quarantine requirements.

(ii) Micropropagation provides a rapid method of propagation. Furthermore, it is dramatic Besides the general importance of providing the means to achieve volume growth, there are also specific benefits. bring points.

Even manual micropropagation methods can produce large amounts of new varieties on a commercial scale. can be achieved in approximately 50% of the time required using conventional methods. Wear.

Equipment that quickly increases the number of new plants, even if they are easily propagated by cuttings. can be done. Even varieties like vines are important.

(iii) Micropropagation methods allow more productive use of space. secondary Culture leads to high utilization of experimental space. Also, greenhouses and plant “hardening” It is also desirable to use the designated space more efficiently. ``Here, do fix'' means seedlings, etc. Gradually expose to cold air to make it stronger, or - firmly root cuttings, etc. Say something. ) When supplying plants to seasonal markets, rapid propagation of each variety is achieved. If possible, leave free space in storage for a long period of time until the number of plants of all varieties increases. There is no need to leave it behind. Operation is based on temperature, light cycle, nutritional balance, etc. It can be continued at any time of the year under controlled conditions.

In addition, to achieve high production volume in time with seasonal demand, the growth rate is adjusted at one time. Storing the medium or lowering the temperature can be used to slow down the process.

To apply the micropropagation method to a specific variety, plant seed material and culture medium (medium) are required. It is necessary to confirm a good relationship between culture conditions and culture conditions. Micropropagation standard The range of plant varieties for which methods have been established is rapidly expanding, resulting in commercial The number of fields in which it can be applied is increasing. Whether in Australia or abroad, The number of seed propagators involved in black propagation has increased significantly over the past six years. this Two main methods have been adopted for this purpose.

The first method is to integrate the cultivation facility into an existing nursery and establish a huge nursery. This is a method to The second method is to establish a culture facility and use the plants in the culture medium to grow other seedlings. This is a method of selling to breeders. At first it was a slow movement, but today Demand from seed propagators and growers for micropropagated plants is rapidly increasing. It's a big deal.

The operating procedure for micropropagation by organ culture is briefly described below.

(i) Early cultivation! 1: Collect new shoots from a specific plant, sterilize the surface, and culture with FA bacteria. Place it on the ground.

(ii) Buds develop from these sprouts, and those buds are secondary cultured to increase the number of sprouts. (SM).

(iii) Buds rapidly grow on the SM and produce numerous clonal sprouts. number of buds In order to increase the number of seeds and maintain the seeds, these buds are subcultured every 3 to 4 weeks to produce new seeds. Creating new SM one after another.

Gv) Some of these buds are subcultured in Petri dishes or similar containers. Root the seeds and use as rooting medium (RM).

Plants obtained in this way are usually collected by stacking the containers containing them. Will be shipped. The contractor who receives the plant removes the plant from the container under sterile conditions and places it under high humidity. ``fixing'' the plant within the temperature environment so that it can withstand outside air conditions.

Secondary culture is usually performed in a transfer room. In other words, it is removed using sterile forceps in the transfer room. The buds are removed from the container using a sterile scalpel, and the buds are usually roughly divided into small buds using a sterile scalpel. Divide into small pieces or individual buds and place on new medium. Sufficient amount of whole meristem Pieces cut so as not to contain the cyst will grow slowly or not at all. Not long. a plant that contains few or no fully differentiated whole cells often do not reproduce as true varieties or as clonal plants.

The biggest cost in the above process is transferring buds from one medium to another. This is the time and effort required for sequential secondary culture. The cost of secondary culture is the same as all other It is at least three times the cost of work. The reason for this is an example of secondary culture of wood. can be explained for. For example, one engineer's daily transfer work The maximum number of operations is s, ooo times. Even if this work is done with the naked eye, Whether done under a microscope, there are similar production efficiency constraints. Furthermore, in the culture laboratory Since sterilization control is often inadequate, losses due to contamination can be substantial. There is a risk of Separation of buds/planting between the planting section, the storage section and the "fixed" section. Cleaning, preparation, control and movement of containers and transfer to “fixation” media also requires labor. It is intensive work.

Therefore, Australian and international efforts to meet the demand for plants through micro-propagation. The seed aquaculture industry's capabilities are limited by the manual techniques currently used in mike field propagation. The cost of partitioning and planting work and the risk of harmful creation even by the most skilled workers. This is difficult due to the fact that there is a considerable limit to the number of tasks that can be carried out per day without It is highly restricted.

SUMMARY OF THE INVENTION It is therefore an object of the present invention to overcome or at least alleviate the above-mentioned disadvantages. It is an object of the present invention to provide a method, method and device for controlling various types of slopes.

8 manifestations of invention Accordingly, in one aspect, the present invention provides for plant species having optically detectable structures. A method of dividing wood according to laws related to the structure of the plant seed material, the method comprising: Scan the material to create an image signal representing its structure, and from the image signal, according to the above law. A method comprising determining a splitting site and providing a splitting signal indicative of the splitting site. It is in.

Preferably, the method of the invention further comprises: The process of processing the image signals and determining the coordinates corresponding to a particular selected part of the structure. and determining the divided portion from the coordinates corresponding to the specific portion. include.

The image signal represents a two-tone image of the plant material as a two-dimensional pixel array. and give each pixel either a background tone or a plant tone. can be done.

The image signal systematically determines the tone imparted to each pixel in the array. process by recording the coordinates of the plant tones and creating said coordinate map. .

For example, confirming the coordinates corresponding to the specific part, such as the tip or node of a branch of a plant. The process is such that the instantaneous contact vixels examined earlier represent the background. This is done by determining whether it represents a plant or a plant.

According to a preferred embodiment of the invention, the tones given to each pixel are systematically looked at. The above process of determining is performed sequentially in two different directions, and confirmation is made in each direction. The average of the coordinates of the specified portion is taken.

In a preferred embodiment, the branches are formed by a predetermined minimum of 20 pixels. Be sure to check the tip of the branch only when the tree has fallen. This makes it very small. This prevents the tip of a branch or a small piece of foreign material from being recognized as a tip of a branch, and In the case of seedlings, each divided part of the plant has a meristem and a well-separated It includes transformed cells.

In another aspect, the invention provides plant seed material having optically detectable structures. A device for dividing plant seed material according to laws related to its structure, the plant material being divided by an image signal generating means for generating an image signal representing the structure of the image; The division part is determined according to the above-mentioned law, and a division signal indicating the division part is supplied. processing means for moving plant seed material at the splitting site in response to the splitting signal; and a cutting means for cutting.

Preferably, the processing means creates a coordinate map of the structure from the image signal; shall serve to ascertain the coordinates corresponding to a particular selected part of the structure. .

In a preferred embodiment, the image signal is transmitted from a microcomputer to an acid processing means. generated by a CCD (IIE charge-coupling device) connected via an interface. I will do it.

The cutting means is mounted on a robot arm so as to be movable above the plant seed material. , a selectively actuatable cutting blade is preferred.

The cutting blade is circular so as to cut a predetermined amount of plant seed material with each operation. Alternatively, it may have an elliptical cross-sectional shape.

An embodiment of the present invention will be described below with reference to the accompanying drawings.

Brief description of the drawing FIG. 1 is a schematic perspective view of a device according to the invention.

This is a full chart of the process.

FIG. 3 shows the flowchart of a pixel processing routine that forms part of the routine shown in FIG. Al on Lee Chart.

Examples of the invention To explain with reference to FIG. 1, the apparatus of the present invention comprises a CC It is equipped with a D camera 11 (hereinafter simply referred to as "CCD"). CCD11 is Thomson C3F CCD camera with standard R3170 video interface It is. CCD11 is a series 1000 model from Corus Data Systems. Oliverati M24 microphone p computer 1 via video capture board 15 6. placed on the continuous belt 13 and scanned by the CCD 11. A signal representative of the tissue of the plant 12 is captured by the board 15, and the signal is digitally After being converted into data, it is downloaded to the main memory of the microcomputer 16. Plant The object 12 is scanned over a background of contrasting color and imaged by the CCD 11. The object 711 is controlled by the microcomputer 16. In this way , CCD 11 is activated and provides a single image of plant 12.

There are 640 digital ratios stored in the microcomputer 12 in the horizontal direction. of a two-dimensional pixel array consisting of 400 pixels in the vertical direction and 400 pixels in the vertical direction. The shape represents a two-tone image of a plant. The tone assigned to each pixel is Corresponds to either ground or plants. The digitized image signal is Systematically determine the tone assigned to each pixel in the array and construct the plant. Processed by

Furthermore, the coordinates corresponding to the tip of the branch and the node to which the branch is connected are checked and the data page is stored in the memory. The systematic processing of each pixel in the pixel array is This was done twice, once using the It will be done. The downscan and upscan routines scan each pixel sequentially. Processing in different directions, the coordinates representing the tip and node of the branch obtained by each routine are The average value is taken.

FIG. 2 is a flowchart of the down scan routine, which scans the image. Starting from its top left corner and working downwards, each pixel is to be processed. The up scan routine (not shown) is also substantially similar to the down scan routine. is the same, but each pixel is processed line by line from bottom to top.

Figure 3 shows the flowchart of the pixel processing routine, which is a subroutine of the down scan routine. It is a low chart. The first step in this pixel processing routine is to This is a backscan of the branch. This is the first pixel that needs to be backscanned. The edge checking technique used in the processing routine is to The cell, the top left pixel, and the top pixel represent the background. This is because it is based on determining whether it represents a branch. Therefore, The branch slanting upward to the left indicates that the vixel directly above the vixel being processed represents a plant. Initially, it is identified as a new branch until it is found to be a vixel.

Once this has been determined, a backscan is performed to capture the plant vixel immediately to the left. , a plant that is on the same branch as the plant vixel that was decreed in 9i to be directly above the vixel being treated. Specify it again as a file.

The remaining steps in this pixel processing routine will be apparent to those skilled in the art from FIG. cormorant.

This device is further operated by a microcomputer 16 to It is equipped with a cutting means in the form of a cutter @g17 for cutting at a predetermined cutting location. Ru.

The cutter mechanism 17 is a cylindrical cutter with a circular cross section installed in a double-acting solenoid 19. It has a blade (cutter) 18. The cutter 18 is a microcomputer 1 6 to cut the plant 12. cut off The solenoid 19 is operated above the plant 12 by two step motors 21. It is mounted on a frame 20 so that it can be moved. 2 step motors 2 1 is a dimension indicating the division part, which is issued from the microcomputer 16. Solenoid 1? are orthogonal to each other direction. In this way, the cutter 18 can move within the range of movement of the frame 20. It can be moved to any position within the cutting area defined by the enclosure. each time The position of the cutter 18 at a point is determined by the number of actuation signals supplied to the step motor 21. and direction by the microcomputer 16 by storing them.

A sensor so that the actual cutter position can be calculated and checked periodically. 22 are provided.

The alignment of the plant 12 under the cutter mechanism 17 is achieved by a continuous belt 13 on which the plant 12 rests. This is done using the reference hole 14 provided in the.

The cutting site determined by the microcomputer 16 is the center of the node and the light of the branch. It is a point 4km inward from the leak.

The cutter 18 has a diameter of 5 m, and a predetermined amount of plant seed material is cut with each operation. It is centered on the cutting site so that Most angiosperms are divided in this way. The cultivated plants contain sufficient meristems and differentiated cells to ensure monoclonal growth. Contains.

Kuniyokuchosai report ANNEX To T) IE INTERNATIONAL 5EARCHRE PORT　NINτERNATIONAL　APPLICATION　No, PC/AU 86100136

Claims (20)

[Claims] 1. Plant seed material having an optically detectable structure is processed by methods related to the structure of the plant seed material. A method of dividing the plant material according to the rules, which scans the plant seed material and uses an image representing its structure. the division part is determined from the image signal according to the above-mentioned law, and the division part is displayed. a method consisting of providing a split signal indicating 2. processing the image signal to create a coordinate map of the structure; a step of confirming the coordinates corresponding to a specific selected part of the structure, and a step corresponding to the specific part; The method according to claim 1, further comprising the step of determining the division site from the coordinates. . 3. The image signal is a two-tone image of the plant material as a two-dimensional pixel array. and assign each pixel either a background tone or a plant tone. 3. A method according to claim 2, characterized in that: 4. The image signal processing step systemically processes tones given to each pixel within the array. By systematically determining the coordinates of plant tones and creating the coordinate map. 4. A method according to claim 3, characterized in that: 5. The step of confirming the coordinates corresponding to the specific portion includes the coordinates of the previously inspected adjacent portion. Whether the pixel represents the background or vegetation The method according to claim 4, characterized in that the method is carried out by determining the . 6. The process of systematically determining the tone imparted to each pixel can be accomplished in two different ways. The coordinates of the specific part confirmed in each direction are 6. A method as claimed in claim 5, characterized in that it comprises taking an average. 7. The above-mentioned specific part is the tip of the branch of the plant seed material and the node where the technique is connected. A method according to any of claims 2 to 5, characterized in that: 8. A branch is formed only when the branch is formed by a predetermined minimum number of roots. 8. The method according to claim 7, further comprising checking the tip. 9. Claim 8, wherein the predetermined minimum number is 20. Method. 10. The divided portion is located a predetermined distance away from the predetermined portion. The method according to any one of claims 2 to 9, characterized in that: 11. plant seed material having an optically detectable structure related to the structure of the plant seed material; A method of dividing according to a law, which method is described herein with reference to the attached drawings. . 12. plant seed material having an optically detectable structure related to the structure of the plant seed material; A device that divides the plant material according to a law, and an image that scans the plant material and represents its structure. an image signal generating means for generating a signal; and a dividing section from the image signal according to the above-mentioned law. processing means for determining the location and providing a division signal for displaying the division location; a cutting means for cutting the plant seed material at the dividing site in response to a dividing signal; A device consisting of 13. The processing means creates a coordinate map of the structure from the health signal, and characterized in that it serves to ascertain the coordinates corresponding to a particular selected portion of Apparatus according to claim 12. 14. The processing means records coordinates corresponding to the coordinate map and the specific portion. The device according to claim 13, characterized in that it includes a database for storing information. Place. 15. The image signal is generated by a CCD (charge coupled device). 15. The device according to any one of claims 12 to 14, characterized in that: 16. The CCD has an interface with a processing means consisting of a microcomputer. 16. The device according to claim 15, characterized in that the device is connected via a network. 17. scanning the plant seed material against a contrasting colored background; 17. The device according to any one of claims 12 to 16, characterized in that: 18. The cutting means is movably mounted above the plant seed material. Claims 12-1 are characterized in that the cutting blade is provided with a cutting blade that can be freely operated. 18. The device according to any of paragraphs 17. 19. The cutting blade is configured to cut a predetermined amount of plant seed material in one operation. 19. The device according to claim 18, wherein the device has a circular cross-sectional shape. . 20. plant seed material having an optically detectable structure related to the structure of the plant seed material; Apparatus for dividing according to a law, which apparatus is described herein with reference to the accompanying drawings .