JPS6242031A - High speed mixing device for response observation - Google Patents

Abstract

PURPOSE:To make a flow line horizontal, and to mix without decreasing the mixing efficiency even in case of any flow velocity by flowing into the remixing distribution passage while the self-agitation is executed, after pre-mixing is executed at the two liquid mixing passage, and further, agitating by contacting to the pin. CONSTITUTION:Different two liquids A and B, which flow together and are pre-mixed at two liquid mixing passages 51 and 52, are mixed once more by connecting with plural distribution passages 61 provided in parallel with the passages 51 and 52. A stick-shaped pin 71 lies at the distribution passage 61, the flow is divided and after detouring, the flow flows together and is agitated. Thus, the two liquid mixing flow to flow into an observing cell 81 to which plural distribution passages 61 are connected is sufficiently mixed without reducing the mixing efficiency by any flow velocity, the flow line of the fluid layer keeps the good horizontal condition and the wire incident surface of the X-ray beam can be held.

Description

[Detailed Description of the Invention] [Industrial Application Field] This invention relates to a high-speed mixing device for reaction observation, which is used, for example, when attempting to detect changes in small-angle X-ray scattering due to changes in protein structure. It is.

[Conventional technology]

Ever since synchrotrons became available for detecting changes in the small-angle X-ray scattering described above, time-resolved X-ray methods have attracted attention, but zero-time-resolved X-ray methods have not yet been put to full-scale use. In order to perform measurements, the incident surface of the X-ray beam of the sample cell must be as wide and flat as possible. Two basic measures have been proposed to meet this condition.

One method is to mix two different liquids A and B fed into two tube-shaped liquid supply passages 1.2 at a meeting point P of the liquid supply passage 1.2, as shown in Fig. 4a. The liquid is injected into one place at the lower end of the sample cell 100 and flows in a planar manner. This method has the disadvantage that the time resolution is reduced because the streamlines are mountain-shaped and not horizontal, as shown by the broken line on the same plane.

The other method is to linearly mix two different liquids A and B fed into two flat liquid supply passages 3.4 at the abutting portion Po of the liquid supply passage 3.4, as shown in Fig. 4b. The mixed solution is injected into the entire lower end of the sample development cell 100 and allowed to flow in a planar manner. According to this method, the flow line of the inflow layer becomes horizontal, so there is no problem like the above method.

Therefore, the inventors of this application have studied various specific devices for carrying out the means shown in FIG. 4b. They are listed below.

(a) Two liquids spread in a planar shape are flowed through a slit and mixed.

(b) A device in which a plurality of small holes are arranged in two rows facing each other, and the two liquids are jetted out from the small holes to mix them.

(C) A device that generates a swirling flow in the ejected two liquids in (bl) above.

(d+In (C1) above, the one that guides the swirling flow into the U-shaped passage and disturbs it.

(1111 In (C) above, the swirling flow is disrupted by applying it to the pin.

However, (a) does not ensure sufficient mixing, and (bl causes an error in the expected reaction rate depending on the flow rate.)
C) showed better mixing than (b), but if there were locations where the two liquids were not sufficiently ejected, the effect could no longer be ignored. (d
) disturbances in the U-shaped passageway are largely meaningless and ineffective.

The above structure had various problems as described above. [Problems to be solved by the invention] In view of the above circumstances, this invention has been developed to create a structure in which two different liquids are sufficiently mixed and their streamlines are horizontal. Another object of the present invention is to provide a high-speed mixing device for reaction observation in which the mixing efficiency does not decrease regardless of the flow rate.

[Means for solving problems]

In order to solve the above-mentioned problems, the present invention provides a plurality of liquid passages in which a pair of two-liquid mixing passages into which two different liquids flow are connected, and divides the fluid into these liquid passages. The gist is that one end of the plurality of liquid passages is arranged in a straight line and connected to one end of the observation cell with a pin for stirring the liquid to be detoured.

[For production]

According to the above means, the two different liquids are premixed both when they flow into the two-liquid mixing passage and when they flow in the two-liquid mixing passage, and then flow into the liquid passage as a swirling flow and are mixed again. Furthermore, this swirling flow hits the pin and is disturbed. Therefore, regardless of the flow rate, the two different liquids are sufficiently mixed before reaching the observation cell, and exhibit a uniform reaction rate. Further, since one end of the plurality of liquid passages is connected to one end of the observation cell in a straight line, the mixed flow of the two liquids flows into the observation cell while maintaining a linear streamline. Therefore, the streamlines maintain a good horizontal state during the flow and when the flow is stopped.

〔Example〕

Embodiments of the invention will be described based on the drawings.

The high-speed mixing device for reaction observation according to the first embodiment of the present invention shown in FIG. 1 includes a pair of U-shaped liquid introduction passages 11.21 that are fitted into each other. The two liquids A and B are introduced separately at a constant flow rate using a piston syringe or the like. A plurality of rising passages 31 . The upper end of 41 is a plurality of two-liquid mixing passages 51
are connected to each. Similarly, liquid introduction passage 1 for liquid A
The other branch flow path 112 in 1 and the liquid introduction path 21 for liquid B
A plurality of rising passages 3 are also provided in the other branch passage 212 in
2.42 are connected to each other, and the upper ends of these rising passages 32.42 are connected to a plurality of two-liquid mixing passages 52, respectively. Therefore, in the pair of two-liquid mixing passages 51 and 52 described above, different two-liquid ASBs are combined and premixed. Further, the pair of two-liquid mixing passages 51 and 52 are connected to a plurality of liquid passages 61 arranged in parallel with each other, so that the premixed two liquids A and B are mixed once again. In this case, as shown in FIG. 3, when the pair of two-liquid mixing passages 51 and 52 are connected to the liquid passage 61 in the connecting direction, the premixed liquid flowing into the liquid passage 61 is mixed while traveling. This further improves the mixing effect.

Further, as shown in the figure, a rod-shaped pin 71 is interposed in the liquid passage 61, and this pin 71 allows the liquid passage 61 to
1 (indicated by arrows) is divided, detoured, and then merged. In the illustrated example, the bin 7j
As is clear from FIG. 1, it is constituted by a single rod-shaped body that passes through all the liquid passages 61. Next, as shown in detail in FIG. 1, the plurality of liquid passages 61 are arranged in a straight line, and their upper ends are connected to the lower ends of the observation cells 81. Therefore, a sufficiently mixed two-liquid mixed flow flows into the observation cell 81 while maintaining a linear spread.

In the two-liquid mixed flow flowing into the observation cell 81 in this state, the streamlines of the fluid layer maintain a good horizontal state, and a wide incident surface of the X-ray beam can be maintained. It goes without saying that the observation cell 81 has a planar internal space.

Further, in FIG. 1, an arrow X indicates the direction of incidence of the X-ray beam, and 82 indicates an observation window formed in the observation cell 81 through which this X-ray beam passes.

In this embodiment, the diameter of the first liquid introduction passage is 2I, the diameter of the rising passage 31.32.41.42 is 0.5 mm, and the diameter of the two liquid mixing passage 51.52 is approximately 1.1 m+m. Moreover, it is desirable to provide at least four or more liquid passages 61.

The difference between the second embodiment of the present invention shown in FIG. 2 and the first embodiment is that the liquid introduction passage 11.2 in the first embodiment is
1. Diversion passages 111.112 and 211.212, rising passages 31.42 and 32.42, two-liquid mixing passages 5 and 52, and liquid passage 61 were formed inside one box. There it is.

More specifically, a liquid introduction hole 11 corresponding to the liquid introduction passage 11.21 that introduces the two liquids A and B in the longitudinal direction of the box body 9.
a, 21a, and grooves 518 and 52a corresponding to the two-liquid mixing passages 51. In the liquid introduction holes 11a, 2
Branches 111a and 112 that communicate orthogonally with 1a, respectively.
a, 211a, 212a, and the two grooves 5
A liquid passage hole 6 is further orthogonal to the hole 9a formed by orthogonally communicating the holes 1a and 52a, and one end thereof is open to the outside of the box body 9.
1a, and this liquid passage hole 61a is connected to an observation cell 81 (not shown).
It communicates with the lower end of the liquid passage hole 61a, and is further provided with a comb 71a-t-inside the liquid passage hole 61a. Incidentally, plate members 91 and 91 for closing the two grooves 51a and 52a are fixed to both side surfaces of the box body 90 by means such as adhesive or welding.

The best material for the box body 9 and the plates 91, 91 in this embodiment is processable ceramics, but metals or plastics may also be used. Moreover, the liquid introduction hole 11a
, 21a has a diameter of 3 mm, two grooves 51a, 52
The size of a is 0.5 n+m in width and 0.5 mm in depth, the diameter of branch holes 111a, 112a, 211a, 212a and hole 9a is 0.5n+m, and the diameter of liquid passage hole 61a is 1
The liquid passage holes 61a are formed at intervals of 0.81 mm, and are formed so that both sides of each liquid passage hole 61a communicate with each other as shown in FIG. do not have.

〔Effect of the invention〕

As is clear from the above, according to the present invention, two different liquids are premixed in the two-liquid mixing passage and then flow into the remixing liquid passage as a swirling flow while self-stirring. It hits and is disturbed. Therefore, regardless of the flow rate, the two liquids are sufficiently mixed before reaching the observation cell to maintain a uniform reaction time, and the mixed flow of the two liquids flows into the observation cell while maintaining a uniform mixing streamline. become.

The high-speed mixing device for reaction observation according to the present invention is not limited to detecting changes in small-angle X-ray scattering due to structural changes in proteins, but can also be used for other applications such as light absorption, X-ray absorption, and circular dichroism. reaction observation, fluorescence measurement, light scattering measurement,
It can be used in a wide range of fields such as reaction observation through conductivity measurement.

[Brief explanation of drawings]

Fig. 1 is a schematic perspective view showing an example of a high-speed mixing device for reaction observation according to the first embodiment of the present invention, Fig. 2 shows the second embodiment of the invention, and Fig. 2(a) shows the box body. Front view, 2nd
Figure (b) is a sectional view taken along line b-b in Figure 2 (a), and Figure 2 (C
) is a sectional view taken along the line c-c of FIG. 2(a), FIG. 3 is an explanatory diagram showing the liquid passage and bottle to which the two-liquid mixing passage is connected, and 4a
FIG. 4 and FIG. 4B are explanatory diagrams showing the concept of mixing fluids in a flat channel. 51.51-two liquid mixing passage, 61-liquid passage, 71-pin, 81-observation cell. Patent applicant: UNITSUKA Co., Ltd. Patent attorney: Mihiko Watanabe Figure 1

Claims (1)

[Claims] (1) A plurality of liquid passages connecting a pair of two-liquid mixing passages into which two different liquids flow are arranged in parallel, and pins for liquid stirring are interposed inside these liquid passages to divert and divert the fluid. A high-speed mixing device for reaction observation, characterized in that one end of the plurality of liquid passages is connected to one end of an observation cell in a straight line.