JPS6229980A - Immobilization of microorganism - Google Patents

Abstract

PURPOSE:To obtain an immobilized microorganism, conveniently applied to farmland without inhalation by operators nor causing any diffusion damage, by kneading an aqueous solution containing a microorganism with calcined gypsum, hardening and solidifying the kneaded material. CONSTITUTION:A useful microorganism is inoculated into an aqueous solution of a culture medium and cultivated by the pure shaking culture method to give a culture fluid, which is then kneaded with powdery calcined gypsum to form a pasty material. The resultant pasty material is then formed into the shape of granules, lumps or powder and hardened to seal up the cultivated useful microorganism in the solidified gypsum.

Description

[Detailed description of the invention] (b) Fields of use of 8 industries and 10 industries The present invention relates to a method for solidifying microorganisms.

(b) Conventional techniques and their problems Conventionally, pure cultured useful microorganisms have been sprayed on farmland or water surfaces for use in soil improvement, sewage purification, etc.

−1= (c) Problems that the invention attempts to solve However,
Pure culture of microorganisms is achieved by pure culture using liquid media, which is inconvenient to transport and spread.
There is a drawback that the microorganisms contained in the atomized liquid medium during spraying can be inhaled by the sprayer or spread, causing harm to others.

(d) Alternative means for solving the problem In this invention, an aqueous solution containing microorganisms is mixed with calcined gypsum, and the microorganisms are added to the calcined gypsum, which is solidified by hardening of the gypsum.
The present invention aims to provide a method for solidifying microorganisms, which is characterized by the fact that only one person can solidify the microorganisms.

(e) Effect In this invention, microorganisms are encapsulated in solidified gypsum, and the gypsum can be formed into lumps, granules, powder, etc. depending on the purpose, so it is convenient for spraying work. Furthermore, it is not inhaled by workers and does not spread, so it does not cause any harm to others.

-2= (f) Effect According to this invention, an aqueous solution containing microorganisms is burned? By kneading with jFf and encapsulating microorganisms in the solidified plaster, it becomes convenient for spraying on farmland, etc., and prevents it from being inhaled by the work noise and from spreading and causing harm to others. There is an effect of this.

(Go to 1) Embodiments The embodiments of this invention will be described in detail as follows.

First, depending on the use and purpose, microorganisms for Ikoi j are inoculated into an aqueous medium solution, pure culture is carried out by a dipping culture method, etc., and this aqueous solution is kneaded with powdered stone powder to form a pace mill. Pour into any mold and harden! This is an ancient study of useful microorganisms cultured in solidified gypsum.

Gypsum, that is, calcium sulfate, is crystallized and stabilized by bonding with six molecules of crystal water, and calcined gypsum is made by heating the above gypsum to evaporate the crystal water at °C and collapse the crystals. It has the property of re-crystallizing when water is added to it, and thus the stone 19 in 1 is solidified by combining many microcrystals, and there are countless microscopic spaces between each crystal. exists, and the microorganisms for tj described in -1- are enclosed in this microspace.

In addition, Pace 1 ~ type plaster after liver pongee white is VI depending on the purpose!
It can be formed into granules, lumps, or powders suitable for cloth.

Note that this solidified gypsum is relatively fragile and easily crushed by external forces, so for example, if it is sprayed on farmland, the gypsum will be crushed by tilling, etc., and the encapsulated useful microorganisms will be released into the soil. It is released into the soil and exhibits the effect of improving soil quality.

In addition, the spaces between the crystals of solidified fossil gypsum are interconnected and continuous, and ultimately communicate with the outside.If moisture exists outside of the gypsum, useful microorganisms will be introduced through the spaces. It is possible to seep out to the outside, and the solidified fossil curd can be molded into a lump that lasts for a long time and is very useful! You can also gradually introduce one thing.

In addition, for the aqueous solution to be mixed with calcined gypsum, useful microorganisms may be cultured and an agar medium or the like may be diluted and dispersed with water.

Claims (1)

[Claims] 1) A method for solidifying microorganisms, which comprises mixing an aqueous solution containing microorganisms with calcined gypsum, and encapsulating the microorganisms in calcined gypsum, which is solidified by hardening of the gypsum.