JPS62286931A - Manufacture of blood serum - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] 3. Detailed description of the invention (a'# I-/7'l III m L% II
IZ1 This invention relates to a method for producing serum that is effective against diseases caused by pathogens in warm-blooded animals.

[Background of the invention]

The object of the invention is to provide permanent or at least temporary immunization against all or a range of diseases caused by pathogens such as viruses, bacteria and fungi; /Or, without worrying about the treatment of diseases that have already appeared, there is a special manufacturing method for treating diseases that have already appeared.

The present invention provides the desired type of human raccoon which, although lacking its susceptibility to warm-blooded animals, can nevertheless have an immunizing and/or curative effect on the human or animal. This is based on the surprising finding that pathogens can be turned off.

[Description of the invention]

The method of the invention involves inoculating a pathogen into a fish protein-containing medium, culturing colonies of the pathogen for the passage passages required to render it non-pathogenic, and at the same time eliminating the need for mutagenic or selection-promoting effects, particularly with short-wave lightning radiation. ．． After sufficient colonization of the culture medium after 60-100 days, a live vaccine is produced directly from the viable pathogen without further processing, and this is inoculated into a warm-blooded animal. - Collect an appropriate amount of blood after 4 weeks,
Serum is obtained by separating plasma, which is a non-coagulable colorless part of blood, and removing fibrin and blood components, and is characterized in that the serum retains immunoglobulin-specific antibodies. That is.

In particular, the serum obtained was found to be useful in combating pathogenic microorganisms.

The inoculum obtained by the method of the invention contains the corresponding pathogen in a form capable of existing under poikilothermic animal proteins. In particular, Staphylococci (Staphy 1ococcl)
For example, Staphylococcus aureus (Slaphy)
lococcus aureus) is important. However, other forms of bacteria that can survive under fish protein, especially
Streptococcus (S jreptococci)
, -z-mococcus (P neumococci),
Bacterium, pyCyaneum, and Corinebacteria, as well as Salmonella and other pathogens can also be used. In addition to fish protein, crustacea, reptiles
, amphibia and insects
Proteins obtained from ts) and worms can be used.

〔Example〕

The present invention will be explained below with reference to Examples.

Example 1 Staphylococcus aureus (Staphyl)
oc.

CCLIS aureus) were widely inoculated into Petri dishes containing normal nutrient agar and cultured in an incubator at 37°C.

After proper growth, some bacteria were transferred to the nutrient medium in a second Petri dish using a platinum wire. This vetri dish is 37
The cells were incubated in an incubator at ℃ and further irradiated with an ultraviolet lamp.

It was found that most of the bacteria could not survive on the medium made of fish protein and died. Only a small number of inocula survived.

After proper growth, some of the cells were transplanted into a third medium using a platinum wire. After incubation, this was subjected to ultraviolet irradiation.

This operation was repeated using new medium each time.

In this example, more than 150 transplants were required;
This does not exclude the possibility that more or fewer transplants could be performed during repeated experiments.

A live vaccine was produced directly from the bacteria growing on the final medium. Experimental animals (mice, guinea pigs, rabbits, etc.)
were inoculated with m of the above vaccine, which was many times the original lethal dose. After 3-4 weeks, especially Staphylococcus aureus
ureus) was raised.

An appropriate amount of blood is collected from this animal, and serum is obtained by separating plasma, which is a non-coagulable colorless part of blood, and removing fibrin and blood substances ([31utk6°rper). specific antibodies remained. Animals treated with this serum were then challenged with the corresponding virulence strain in untreated controls. Inoculated animals were completely immune despite injections q many times the lethal dose for non-inoculated animals. Immunity in domestic rabbits can be assayed by Ophthalony immunoprecipitation.

Example 2 Staphylococcus (S. Laphylocci) was grown live in a Petri dish containing an agar medium similar to Example 1.
·Ta. As in Example 1, the cells were grown in another medium containing fish protein and transplanted again. To promote mutation, we irradiated them with long-wavelength X-rays. A live vaccine was produced from the treated f.

The tests according to Examples I and 2 were carried out on Streptococci (
S t repLocc i), especially Streptococcus hemolydicus
haemolyticus), as well as BacLcriumpyocyaneum
um) and Corynebacterium nophtheriae (Cor
ynebacterium diphteriaa)
, and obtained similar results. Additionally, good results have been obtained using fresh or undenatured protein from pond fish, especially freshly harvested or freshly frozen fish. The protein can be used in the form of a suspension, solution or dispersion. By appropriate adjustment of culture conditions and irradiation intensity, it is possible to generate corresponding non-pathogenic variants from all previously known or possibly unknown pathogenic bacteria, and for viruses, fungi and microplasmas. The same is true. Of particular importance is the possibility of therapeutic treatment in warm-blooded animals already suffering from the disease.

Patent applicant: Niggheld Freiherr Fono Marzen-Bonikau

Claims (1)

[Claims] (1) Inoculating the pathogen into a fish protein-containing medium, culturing the pathogen colony for the passage passages required to render it non-pathogenic, and at the same time exposing the mutation or selection-promoting influencing factors to particularly short-wavelength electromagnetic radiation; After the final medium has been sufficiently colonized, a live vaccine is produced directly from the live pathogen without further processing and inoculated into a warm-blooded animal, from which a predetermined amount of blood is collected after 3-4 weeks. The method is characterized in that serum is obtained by separating plasma, which is a non-coagulable colorless part of blood, and removing fibrin and blood components, while leaving only immunoglobulin-specific antibodies in the serum. , a process for the production of serum based on pathogens that are non-pathogenic to humans and warm-blooded animals.