JPS62249911A - Skin cosmetic - Google Patents

Abstract

PURPOSE:A skin cosmetic which has improved water retaining function (improving effects on chapped skin, improving effects on keratin and humectant effect) of skin, improved skin beautifying effects (organoleptic test) and improving effect on chapped lip, etc., using both water-soluble collagen and mucin. CONSTITUTION:A skin cosmetic such as lip cream, skin lotion, skin cream, etc., is blended with (A) preferably 0.2-10wt% based on total amount of the cosmetic of water-soluble collagen and (B) preferably 0.2-10wt% mucin in a weight ratio of the component B:A of 20:80-70:30 to give a skin cosmetic which improves water retention function of the skin, skin beautifying effects to provide the skin with wetting properties (wetting feeling), flexibility (smoothing feeling), elasticity and gloss and yet low skin irritation.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of the Invention) The present invention relates to the use of water-soluble collagen and mucin.
This invention relates to a skin cosmetic that improves the moisture retention function of the skin (improving rough skin, improving keratin, and moisturizing effect) and exhibits beautifying effects.

(Prior art) In order to maintain healthy and beautiful skin, hydrophilic skin moisturizers and oily skin softeners that provide appropriate moisture and oil to the skin have traditionally been blended into skin cosmetics. There is.

Skin moisturizers include glycerin, propylene glycol,
Polyethylene glycol, virolidonka sb &'/
1m N Decoration + (Bl field!
-n, A J-? - It may absorb moisture from the stratum corneum, which is the outermost layer of old skin, and may cause a loss of moisture in the skin.In addition, skin cosmetics containing polygonum may cause discomfort such as stickiness. However, it is not always satisfactory.

In addition, liquid paraffin, petrolatum, olive oil, squalane, lanolin, synthetic ester oil, etc. are used as skin softeners, but these are also included in skin cosmetics to the extent that they sufficiently prevent water evaporation from the epidermis. In some cases, they have disadvantages such as inhibiting the normal metabolism of the skin.

(Disclosure of the Invention) In view of the above-mentioned drawbacks of skin moisturizers and emollients, the present inventors have developed a method that maintains the skin in a healthy state and continuously improves the moisture retention function of the skin. As a result of intensive research aimed at providing skin cosmetics, we found that the skin cosmetics containing Takeyodo's mucin and water-soluble collagen described later exhibited effects that met the purpose, and also had moisturizing properties on the skin. The present invention was completed based on the discovery that it has a beautifying effect on the skin, giving it a moist feel, flexibility (shiny feeling), elasticity, and luster.

(Objective of the Invention) The object of the present invention is to provide moisture retention function of the skin (improving effect on rough skin,
Our goal is to provide skin cosmetics with excellent keratin-improving effect, moisturizing effect) and skin-promoting effect (sensory test).

(Structure of the Invention) The present invention is a skin cosmetic containing water-soluble collagen and mucin.

(Specific explanation of the structure) The water-soluble collagen used in the present invention is a protein with a molecular weight of 10,000 to 500,000 that is extracted using a known liquid or an operation such as protease treatment and purified through a deodorizing and decolorizing process. It is a substance that is the main ingredient. Other components may include sugars such as mucopolysaccharide and glycogen, and nucleic acids.

In the present invention, the amount of water-soluble collagen blended is preferably in the range of 0.2 to 10 wt% based on the total amount of the skin cosmetic. If the amount is less than 0.2 wt%, the effect will not be sufficiently achieved, while if it exceeds 10 wt%, no improvement in effect commensurate with the increase can be expected.

The mucin used in the present invention is a known substance, and generally refers to a highly viscous sticky substance produced by animal epithelial cells, mucous membranes, salivary glands, etc., and can be stably obtained industrially. is a viscous substance obtained from the stomach or saliva of mammals (especially cows and pigs).

The percentage calculation method may be based on, for example, the description in US Pat. No. 2,805,714. Examples thereof are described in the Experimental Examples below.

(Experimental example) (1) Remove the stomach from a pig immediately after slaughter and remove its contents.

(2) After separating the gastric fundus lining from the obtained stomach and removing the non-stomach fundus, 8.4 ky of gastric mucosa is obtained.

(3) The gastric mucosa obtained in (2) is placed in a glass-lined tank containing 10 times the amount of 0.5% hydrochloric acid solution, stirred for a short time at room temperature, and pulverized.

(4) Since a solution part and a precipitate part are generated, a clear filtrate (81n) is obtained by filtration.

(5) Add sodium carbonate to the filtrate obtained in (4) to adjust the pH to 4.5, then add the same amount of ethanol as this aqueous solution and leave it at 4°C overnight.

(6) Wash the obtained precipitate with water several times.

(7) Add acidic sodium carbonate water and adjust the pH to 7.0.

(8) Dry the aqueous solution by spray drying to obtain 65f powder (pig stomach mucin).

In addition, cow saliva 41 was collected and used in (5) of the above experimental example.
) After applying the same treatment as above, the cow saliva mucin 61. O
I got f.

The characteristic values and analysis values of each mucin obtained above are
Record in the table.

Table 1 In the present invention, the amount of mucin blended is preferably in the range of 0.2 to 10 wt% based on the total amount of the skin cosmetic. If the amount is less than 0.2 wt%, the effect will not be sufficiently achieved, while if it exceeds 10 wt%, no improvement in effect commensurate with the increase can be expected.

It is presumed that the skin cosmetics of the present invention form a unique gel matrix by blending water-soluble collagen and mucin, forming a highly moisturizing film on the skin surface. It shows a clearly superior skin moisture retention function compared to skin cosmetics formulated alone.

Moreover, the blending ratio (weight ratio) of mucin and water-soluble collagen suitable for forming this gel matrix is mucin:water-soluble collagen=20:80 to 70:80.

In addition, in addition to the above, the skin cosmetics of the present invention may contain pigments, fragrances, preservatives, surfactants, humectants, pigments, antioxidants, etc. as appropriate within the scope of achieving the purpose of the present invention. can.

(Example) Hereinafter, the present invention will be explained in detail based on Examples and Comparative Examples.

The moisturizing effect test method, rough skin improvement effect test method, keratin improvement effect test method, sensory test (beautifying skin effect test method), and lip balm practical test are as follows.

(1) Test method for improving effect on rough skin The effect of continuous application for 4 weeks was investigated on 20 middle-aged and elderly subjects who had rough skin on their lower legs. Approximately 1 V of the sample was applied to the test site of the left lower leg of the subject once a day, and the condition of the skin before and after the test was judged according to the following criteria.

No sample was applied to the right lower leg, which served as a control.

Judgment criteria for skin dryness - 2 Normal ±: Slight dryness, no scaling 12. Dryness, mild scaling 12. If the skin dryness has improved by two or more levels (e.g. +→-1)
10→±) is valid, one level improvement is slightly valid,
If there was no change, it was considered invalid. The test results are shown as the number of subjects who found the drug to be effective or somewhat effective.

(2) Effect test method for improving keratin (increasing anti-peeling properties of keratinocytes) When Scotch tape (Tiban mending tape) was adhered to the skin of the test area before and after the start and end of the rough skin improvement measurement test described above, and this was peeled off. The condition of the corneocytes attached to the tape was examined in detail using a scanning electron microscope, and the anti-desalination property of skin corneocytes was analyzed according to the following criteria to determine the corneum improving effect.

Judgment criteria for keratin improving effect (increased anti-desquamation property of keratinocytes) Evaluation score: 1 No scale observed 2 Small scale scattered 8 Small to medium scale noticeable 4 Large scale noticeable evaluation is the evaluation score of the test area after 4 weeks of continuous application. A difference of 2 points or more from that of the control site was considered valid, a difference of 1 point was considered somewhat effective, and a difference of 0 points was considered invalid.

The judgment results are expressed as the number of subjects who found the test to be effective or somewhat effective.

(3) Moisturizing effect test method Before and after the start and end of the rough skin improvement effect test, the skin conductance value (unit: micromho) of the test site of each subject was measured using an impedance meter (1, B, manufactured by S Company, l
B5-854 type).

It is generally accepted that the higher the skin conductance value, the lower the electrical resistance of the skin and the higher the stratum corneum moisture content of the skin.

The moisturizing effect is determined by the rate of increase in stratum corneum moisture (%) calculated using the formula below.
) was evaluated.

Corneum moisture increase rate (%) - y 2" low x to.

W.

Wo: Conductance value W of the sample application site before the start of the test
:〃 〃 At the end of the test 〃 The test results are shown as the average value of the stratum corneum moisture increase rate of 20 subjects.

(4) Sensory test (skin beautification effect test) Female subjects (85 and up) who complain of rough skin, fine wrinkles, dry skin, etc.
The sample was applied to 20 people (55 years old) twice a day (in the morning) for 8 consecutive months, and the effects were evaluated after 8 months.

The test results showed that the skin was moisturized, the skin was smooth, and the skin was moisturized, smooth, and elastic.
It is shown by the number of people who answered that their skin became taut.

(5) Lip balm practical test Twenty female subjects complaining of chapped lips conducted a practical test once a day for one month, and answered the following questionnaire.

0 Effect on chapped lips Compared to before the test, judgment will be made based on whether or not the chapped lips have improved (cured ¥1).

0Effect on lip gloss The test will be based on whether or not your lips are glossier than before the test.

Examples 1 to 4, Comparative Examples 1 to 4 [Two-layered skin low silane] Pig stomach mucin, cow saliva mucin, and the water-soluble collagen obtained in the experimental examples as shown in the following composition are listed in Table 2. Each skin low silane was prepared by blending as described above, and the various tests described above were conducted.

(]) Composition (2) Preparation method After each of the components (A) and CB) is uniformly mixed, the components (A) and (CB) are mixed and dispersed with stirring, and then filled into a container.

When using, shake and disperse the contents uniformly.

(3) Characteristics The results of various tests conducted on each two-layer skin low silane are listed in the right column of Table 2.

As shown in Table 2, the skin-low silanes of Examples 1-4 of the present invention were found to have superior properties in various tests compared to the skin-low silanes of Comparative Examples 1-4. Furthermore, in Comparative Example 4, in which gum arabic other than the water-soluble collagen according to the present invention was blended, the desired effect could not be obtained.

Examples 5 to 7, Comparative Examples 5 to 7 [Skin cream] In the same manner as in Example 1, each skin cream was prepared with the following composition and various tests were conducted.The results were summarized as (1) composition (2) Preparation method Component (4) and component (R) were each heated and dissolved at 80°C, mixed, and cooled to 30°C with stirring to prepare each skin cream.

(3) Characteristics As shown in Table 2, the skin creams of Examples 5 to 7, which are skin cosmetics of the present invention, have a rough skin improvement effect and a keratin improvement effect compared to the skin creams of Comparative Examples 5 to 7. [Lip balm] (1) Composition (2) Preparation method (A) component and 03) component each heated to 80°C After dissolving, each lip balm was prepared by mixing and cooling to 80°C while stirring.

(3) Results Table 8 From the results, it was confirmed that by using mucin and water-soluble collagen in combination, these ingredients act on the mucous membrane of the lips, improving roughness and imparting gloss.

(Effects of the Invention) As described above, the present invention has excellent skin moisture retention function (improving rough skin, keratin improving effect, moisturizing effect), beautifying skin effect (sensory test), improving effect on chapped lips, etc. ,
It is clear that a skin cosmetic with low skin irritation is provided.

Claims (1)

[Claims] (1) A skin cosmetic containing water-soluble collagen and mucin.