JPS62244437A - Gel preparing base material - Google Patents

Abstract

PURPOSE:To prepare only a necessary amount of a gel at ambient temp., by adhering a gelatinization promoter such as calcium gluconate to a base body such as a laboratory dish and subsequently injecting an aqueous solution of alkali metal or alkaline earth metal alginate in said dish. CONSTITUTION:A gelatinization promoter 4 for gelatinizing an aqueous sodium alginate solution is applied to the inner wall of a laboratory dish 2 and dried. The aqueous sodium alginate solution 12 is injected in the laboratory dish 2 and allowed to stand to complete a gel 14. As the gelatinization agent, calcium gluconate is pref. and filter paper impregnated with the gelatinization agent may be placed on the bottom of the laboratory dish. By this method, because the gel can be prepared at ambient temp. unlike an agar gel, blood weak against high temp. can be mixed with a medium and the medium having pH over a wide pH-range of 3-10 can be obtained.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a gel production base material for producing glue by solidifying a sol into a sherry form.

(Prior Art) Agar is a typical example of a gel used as a culture medium for microorganisms. When agar is used as a culture medium for microorganisms, the agar powder is first placed in water along with the nutrients necessary for culturing, and then heated to approximately 100°C for dissolution. Next, this aqueous solution is sterilized and placed in a container such as a petri dish before the temperature reaches 40° or more.

When it is then cooled, it solidifies and forms gelatinous agar.

Thereafter, the microorganism to be cultured is inoculated into this agar.

(Problem to be solved by the invention) In this way, conventionally, agar powder was dissolved in water, heated once, and then cooled to make an agar medium.
There was a problem in which more amount of agar than necessary was formed on the melon. Therefore, C, for example, 3 petri dishes'? ) Even if agar was needed, a large amount of agar was produced and it was wasted.Also, it was difficult to make agar easily in a short time because it had to be heated and then cooled. There was a problem.

Furthermore, substances that are destroyed at high temperatures due to heat treatment, such as blood, cannot be used as additives or nutrient sources.1. , it was r. In addition, in the case of agar, it is difficult to avoid solidification when p l-1 is less than 5, so there is a limit to the pH value f1 of the culture medium.

The purpose of the present invention is to produce a gel that can be easily produced in an amount necessary for 171 flights.

(Kth means for solving the problem) Achieving the above object V) The glue production base material according to the present invention contains a gelling promoter that gels an aqueous solution of an alkali metal or alkaline earth metal salt of alginic acid. It is characterized by being attached to a substrate.

The substrate is a manufacturing container for slicing the gel, and it is desirable that the gelling promoter be exposed on the inner wall surface of this manufacturing container.

Further, it is preferable that the substrate is [, 1 Sea 1~], and that the gelation accelerator is contained in this Sea 1~.

It is desirable that the gelation promoter is calcium gluconate.

(Detailed Description of the Invention) A glue manufacturing substrate according to the present invention is shown in FIG. This glue production base material is characterized by having a gelation accelerator 4 applied to the inner wall surface of a Petri dish 2 made of glass, plastic, etc., which is a container with an 'rJ container with a lid, to avoid gelation of a ditrium alginate aqueous solution. There is. The gelling promoter 4 may be any material that slowly releases calcium divalent ions (ca2'') when dissolved in the sodium/lium alginate aqueous solution.

The method for manufacturing this gel @base material will be explained using FIG. 2. First, prepare a washed and sterilized sheet A-2 (second
Figure (a)). Next, put a beam 8 into the peeling force 6 containing the liquid gelation accelerator 4, impregnate the beam 3-8 with this gelation accelerator 4, and apply it to the inner wall surface of the seal 2. (Figure 2(b)). The gelling promoter 4 is applied to the entire inner wall surface of the layer 11-2 using the brush 8 and then dried (FIG. 2(C)). This makes this gel
1i base material is completed.

A gel manufacturing method using a gel manufacturing base material will be explained using FIG. 3. First, a gluing promoter 4 is applied to the inner wall surface of a petri dish 2.
Prepare a base material for gel production (Fig. 3(a)).

Pour 1 to 12 alginic acids into Petri dish 2 at the 7th minute from a beer strength of 10 (Fig. 3(b)). then t
When the gel 14 is placed in the Petri dish 2, the gel 14 is completed.
Figure (C)).

Sigma's 1'ypclV (trade name) was used as an aqueous solution of sulfur and lithium alginates when producing glue.

Sigma's rypc
The main component is sodium alginate, which is produced by separating, extracting, and refining B. pyritcra, and contains impurities such as calcium. The aqueous solution of helium alkinate to be used is preferably about 0.5 to 2.0%. When used as a culture medium for microorganisms, culture in this 0.5 to 2.0% aqueous solution of sodium alginate. Mix nutrients suitable for the microorganisms and bacteria to be grown and a buffer for adjusting PL. As for nutrition, ■Pair 1~
■ yeast extract, ■ bouillon (gravy), ■ sugars, ■ amino acids, and ■ blood.

M@ agents include ■Potassium phosphate and ■Thorium phosphate.When the nutrient is other than blood, the above mixture is usually sterilized under high pressure.

When calcium gluconate is used as a gelation promoter, 1.8 to 2.2 of calcium gluconate (4.5% aqueous solution) is substantially Apply a material that dissolves in water on the inner wall surface of Petri dish 2. That is, 1!M of 1% sodium alginate (Sigma type ■) is coated with 80%
~100 μg of calcium gluconate may be applied to the inner wall surface of Petri dish 2. To actually apply it to Petri dish 2, add a glue to the calcium gluconate aqueous solution to make it easier to apply. Glue As a food, Zeraan or No'
Water-soluble rubber such as severe rubber is preferable. 1. Tapori Oni 1
Add a surfactant instead or together with glue Y1 (b3
],stomach.

In addition, according to the findings of the present inventors, if calcium gluconate is present, it is assumed that glunic acid plays a role as a crosslinking agent when gelling mono-alginic acid. .

In addition to the above-mentioned method of applying the gelling accelerator 4 to the Petri dish 2, there are the following methods.31 For example, as shown in Fig.・1st (applying method C is also good.

In other words, prepare a sterilized shell 11-2 as shown in Figure 3 (a).
Next, apply the gelling accelerator 4 to the nozzle 1 of the mantsugo spray 16.
8 to spray and coat the gelling promoter 1 on the inner wall surface of the tray 2 (FIG. 3(b)). Then dry V-
The C gel production base material is completed (Figure 3 (C)). Also, gelation (jf propellant may be mixed into solid paste and coated on the inner wall of Petri dish 2 (1°)) In addition, gluconic acid may be used as a gelation promoter instead of calcium gluconate. You can also apply it with a solid paste.

Another example of the gel production base material according to the present invention is shown in FIG. This gel production base material is made by impregnating a circular filter paper 20 as a base material with a gelling accelerator for gelling an aqueous solution of helium alginate.

The method for producing this gel production base material is shown in FIG.

First, place multiple pieces of filter paper 20 in a large petri dish 22 (
Figure 6(a)). Next, calcium gluconate 24 is poured into this petri dish 22 from the beaker 26 (FIG. 6(b)).

Next, the paper 20 impregnated with calcium gluconate 24 is taken out from the tray 22 and dried (Fig. 6 (C)).
. A filter paper 20 impregnated with calcium ligulinate is completed.

The gel manufacturing method using this gel manufacturing base material will be explained using FIG.
8 (FIG. 7(a)) Next, calcium gluconate 31 is poured into this petri dish 28 from the beaker 32. After that, if you leave it alone, the glue 36 will be completed in the petri dish (
Figure 7(C)).

Even when using the J-gel production base shown in FIG. 5, the sotrium alginate aqueous solution preferably has a concentration of about 0.5 to 2.0%. The point that nutrients and buffers are used as necessary is the same as in the case of the J-glue production base shown in FIG.

Note that instead of the circular filter paper 20, a square filter paper impregnated with calcium gluconate may be used, and the filter paper may be cut into the shape of a manufacturing container such as a Petri dish for the purpose of use. 2
1.l The liquid soaks in and is retained instead of the rolling paper.
Other fibrous substrates such as cloth may also be used.

B made by Guru according to the present invention 2. When using the moon as a culture medium for microorganisms, the following advantages are shown.

(2) Unlike conventional agar, the gel manufacturing base material of the present invention can be used to produce up to 18 times the required amount. Z = 8
- In other words, all you have to do is prepare the necessary gel production base material and pour sodium alginate into it.

(2) When using the gel production mill of the present invention, the gel can be produced at room temperature without heating, so blood, which is sensitive to high temperatures, can be mixed into the culture medium as an additive or nutrient.

■ When using the gel production base material of the present invention, by adjusting the suspension of the buffer, a wider range of p
]-1 culture medium can be obtained. l) H3 to 10
It is possible to adjust between.

■ The gel made from the gel production base material of the present invention has high strength and is easily coated with microorganisms and bacteria.

The gel production base material of the present invention has various uses other than culture media as shown below.

(1) Zone electrophoresis analysis method This zone electrophoresis analysis method is a method in which a solvent is held on a non-conductive bamboo solvent-insoluble bamboo support and electrophoresis is caused by placing the solvent in an electric field. Confirmation of the migration zone [This is done in the same way as in the case of Komatogelahu. Among these zone electrophoresis analysis methods, Hirane electrophoresis uses a plate-shaped support for electrophoresis. This support gel! The gel manufacturing base material of the present invention can be used for manufacturing. A square tray is used to produce a plate-shaped gel. When the gel manufacturing material of the present invention is used, there are the following advantages: (1) The gel required for analysis can be produced. ■The range of pl-1 can be widened, so the range of analysis can be expanded.■There is no need to take into account temperature regulations, as arbitrary depletion can be performed.

(2) Immunoelectrophoresis is a method that combines electrophoresis and in-gel immunodiffusion. For example, if serum is placed in a hole in a gel plate with a thickness of 1 to 1.2 mm and separated by electrophoresis, and antiserum is placed in a groove parallel to the direction of electrophoresis and allowed to react, antibodies corresponding to each lυ exposure component will be detected. It diffuses and reacts, forming an arc-shaped sedimentation line at the optimum ratio. This removes multiple protein components. The gel of the present invention is used to manufacture this gel deck! i Construction W forest can be used. When using the gel production base material of the present invention, there are the following advantages.

■It's easy to make the required amount. ■Even if the humidity is low: Yes.

(3) - Original radial immunodiffusion method This - original radial immunodiffusion method involves mixing the antigen or antibody with agar or agar to prepare a gel plate containing the antigen or antibody, with certain small pores. An antibody or antigen solution is injected into this hole and allowed to react at a certain temperature for a certain period of time.

The injected antigen or antibody diffuses concentrically within the gel,
Antigen-antibody reaction produces white precipitation. A certain proportional relationship holds between the magnitude of this precipitation and the antigen or antibody concentration. Utilizing this fact, the gel production base material of the present invention can be used to manufacture the gel plate on which the quantitative determination of immunoglucose [-1 purine, etc.] is carried out. When using the gel production base material of the present invention, there are the following advantages. ■It is possible to make the required amount.■Unlike agar or agarose, there is no need for heating when preparing gel plates, and denaturation of antigens or antibodies due to heating can be prevented.

(4) Food By preparing a gel-made 1/M IHL +g coated with a gelling accelerator on the inner wall of a container with a predetermined shape, you can add 1" forestry material, 1") fruit" to this container. Immediate jelly can be made by injecting mono-lium alginic acid mixed with 21 sulfur, etc.

(Example) Hereinafter, the present invention will be explained in more detail with reference to Examples.
The present invention is not limited to these examples.

Example 1 Alginate (~Rium Toshi-C Sigma Reiper)
A 1% aqueous solution of V (trade name) was used. A 2% aqueous solution of calcium gluconate was used as a gelation promoter. Diameter 9
Only 2 volumes of the above calcium gluconate were placed in the cfR cylinder, and 15 ml of the above-mentioned alginate was injected into it. After that, a sufficiently strong gel was formed after being left for 3 hours.

Example 2 A coating solution for applying calcium gluconate to paint was prepared as follows.

An aqueous solution of 100 cc of water was heated to 100° C., and 20 g of calcium gluconate was dissolved therein. Further, 15 g of gelatin was dissolved as a thickening agent and left to stand at room temperature. When the coating solution reached about 50° C., it was applied to the inner wall of a Petri dish using r.g., t&p. It dried approximately 30 minutes after application, and a gel production base material was completed.

A 1% aqueous solution of sodium alginate (Sigma Rypc+V>) was injected into this gel production base material for 15 minutes and left to stand for 3 hours. As a result, a gel was completed in a petri dish.

Example 3 A filter paper was placed in a petri dish, and a 4.5% aqueous solution of calcium gluconate was injected into the dish so as to evenly soak into the filter paper. After drying the paper, a gel production base material was produced.

Place the paper impregnated with calcium glunate in a petri dish, and add sodium alginate (Sigma T.
ypelV) was injected for 15 m. After that 2 hours several iFi
The gel was successfully completed in the petri dish.

(Effects of the Invention) As described above, according to the present invention, the necessary hanging gel can be easily produced.

[Brief explanation of drawings]

Figure 1 is a perspective view showing the gel production base material of the present invention, Figure 2 is a process diagram showing the method for manufacturing this gel production base material, and Figure 3 is a diagram showing the gel production method using this gel production lj material. Covering process diagram,
FIG. 4 is a process diagram showing another method of manufacturing this gel manufacturing base material, FIG. 5 is a perspective view showing another example of the gel manufacturing base material of the present invention,
FIG. 6 [Process diagram showing the manufacturing method of Mako's gel manufacturing base material,
FIG. 7 1.1 is a culm diagram showing the gel production method using this gel production base material. 2...Sithole, 1...Gelification accelerator, 6...Beer force, 8...Hake, 10...Beer force, '12...
Al-1! Notorium phosphate aqueous solution, 1/l...gel,
16... Spray, 18... Nozzle, 20... Filter paper, 22... Petri dish, 24... Gelling agent, 2 (5
... Bee force, 28... Petri dish, 30... Pinhit, 32... Bee force, 3/1... Alginic acid] 1
Helium aqueous solution, 36...gel. Applicant's agent Sato - Iroishi 1 Figure also 3 Figure (G) 2 翫しl し==Go. L==Go' 4 Figures 5 Figures 6 Figures 7 Mouth

Claims (1)

[Scope of Claims] 1. A gel production base material, in which a gelation accelerator for gelling an aqueous solution of an alkali metal or alkaline earth metal salt of alginic acid is attached to the base. 2. In the gel production base material according to claim 1, the base body is a production container for producing gel, and the gelation accelerator is attached to the inner wall surface of this production container. Characteristic gel manufacturing base material. 3. The gel production base material according to claim 1, wherein the base body is a sheet, and the gelation accelerator is contained in this sheet. 4. The gel production base material according to any one of claims 1 to 3, wherein the gelation promoter is calcium gluconate.