JPS62234026A - Food for patient of hepatic function disorder - Google Patents

Abstract

PURPOSE:To provide the titled food effective in improving symptoms of hepatic function disorder and supplying nutrient and composed of an edible medium containing basidiomycete of Fomes genus and cereals, beans, etc., and beta-D- glucan, saponin and ergosterol produced by fermentation of the medium. CONSTITUTION:A basidiomycete of Fomes genus, preferably mycelium of Fomes japonicus is cultured in a liquid medium composed of a carbon source such as cereals or potatoes and a nitrogen source such as beans. The fermentation product produced by culture is properly dehydrated and used as a fermented food for the patient of hepatic function disorder. The food has a symptom- improving effect comparable to or higher than that of basidiomycete belonging to Fomes genus and improves the palatability of the fungus. Furthermore, it can be used for the purpose of preventing hepatic function disorder such as hepatitis, etc., improving the diet, maintaining the health and improving physical strength.

Description

[Detailed description of the invention] (Industrial application field) The present invention relates to a food for people with liver dysfunction.

(Prior Art) Liver function diseases include hepatitis including viral hepatitis and alcoholic hepatitis, liver cirrhosis, liver failure, and the like. Viral hepatitis, alcoholic hepatitis 4 When the condition worsens, it progresses to chronic hepatitis and even cirrhosis. Liver cirrhosis always carries the risk of death due to liver failure or gastrointestinal bleeding.

For the purpose of preventing and improving liver dysfunction, many attempts have been made to solve these serious problems not by administering pharmaceuticals, but by focusing on single meals. For example, there are ``high-protein foods'' that are ``special nutritional foods'' stipulated in the ``Nutrition Improvement Act,'' and so-called ``health foods.''

The ``Nutrition Improvement Act'' aims to ``enhance the nation's idea of improving nutrition.

Its purpose is to clarify the nutritional status of the nation, take measures to improve the nation's prosperity, maintain and improve the health and physical strength of the nation, and thereby contribute to the promotion of the nation's welfare. Article 1 of Thought).

There are ``high protein/X foods'' that are suitable for diseases such as liver diseases that require increased protein intake.

"Health foods" include extracts of physical and animal ingredients, vitamin supplements, etc. These products allude to nutritional support and health promotion through specific nutrients.

An example of using an extract is a "food containing Jiaogulan saponin" which contains Jiaogulan saponin as an essential component (Japanese Patent Application Laid-Open No. 60-45558@i). It was a cabinet. "Food containing Reishi extract" which has Reishi extract as an essential ingredient
(Unexamined Japanese Patent Publication No. 60-34914: Ri) eh (Problem to be solved by the invention) The conventional "high-protein food" of the "special honor food" is.

It should only be used under the guidance of a doctor or nutritionist if a doctor has instructed you to consume a large amount of protein, such as in patients with liver disease. "High-protein foods" are those that contain protein - more than twice the amount of normal similar foods, and consuming large amounts of these foods may cause various adverse effects. be. In other words, it merely halts the progression of the disease and is not intended to actively improve the condition. However, since it is a single food, it is not nutritionally balanced.

Many of the so-called "health foods" have unclear ingredients, and even if they are labeled to suggest that they have an effect on improving disease conditions, the existence of active ingredients that have the effect of improving disease conditions is unknown, and it is not clear whether they are effective in improving disease conditions. The effects are also unclear. Even for ``health foods'' whose ingredients have been analyzed, there are many questions about their nutritional balance as foods for daily consumption, the content of active ingredients that have the effect of improving pathological conditions, and the stability of their quality.

For example, [foods containing Jiaogulan saponin] (t￥f
Publication No. 60-45558) is a food containing Jiaogulan saponin extracted from Jiaogulan as an essential ingredient. +, Although pharmacological effects such as the effect of suppressing the metabolism of platelet arachidonic acid can be expected, other nutritional components and nutritional balance necessary for improving the pathogenesis are unknown and have not been taken into account.

Mayu, ``Food J containing Seiun extract ('% Kaian 60-
34914) is a food whose essential ingredient is the FCF+iI product obtained by further extracting the hot water extract of the fruiting body of Futoshiba with an oxygen-containing organic solvent, and it is expected to have pharmacological effects such as the suppressing effect on arachidonic acid metabolites. However, the other nutritional components and nutritional balance necessary to improve the disease are unknown and have not been taken into account.

The present invention solves the above-mentioned problem 1 by providing a food for people with impaired liver function that has both the effect of improving the pathology of liver dysfunction and the effect of supplementing nutrients to reinforce this.

(Means for solving the DfU problem) The food for people with liver dysfunction of the present invention contains
It consists of basidiomycetes, cereals or potatoes, beans, β-D-glucan, and saponin.

Using fermented foods containing ergosterol
This is a food for people with liver dysfunction that is characterized by:

The present inventors (2E) took into account various problems seen in rice's "special nutritional foods" and "health foods," focused only on nutritional components, and Rather than mixing and FC-containing the obtained extracts containing the active ingredients to produce a "health food", we developed the product with the aim of obtaining a food for improving pathological conditions. The basidiomycetes and cereals that belong to this group are more productive than potatoes and legumes.

The present invention was made because a fermented food containing β-D-/lucan, saponin, and ergosterol showed an effect on improving the pathology of patients with liver dysfunction.

In addition, the food for people with liver dysfunction according to the present invention contains protein.

It also contains a well-balanced variety of nutritional components, including carbohydrates, and has the effect of improving disease conditions.

It can also be designated as a "special honor food."

The basidiomycete of the genus Cynthia has been known for a long time to be useful for the body, and it has been known that it is effective in improving liver function and is also effective against arteriosclerosis.

The basidiomycetes of the genus C. monocytogenes used in the fermented food used in the present invention are: C. monocytogenes.

It is the mycelium of a basidiomycete such as Physcomitrella chinensis, Physcomitrella chinensis, and Streptococcus chinensis, and preferably the cheloid body of Physcomitrella ultifolia is used.

The fermented food used in the present invention can be obtained by culturing a basidiomycete belonging to the genus Hemorrhoids in a medium using type 9A or potatoes as a carbon source and beans as a nitrogen source.

The grains, potatoes, and beans that are the culture medium components should be selected from the food groups listed in the Food Composition Table of Japanese Foods [Japan Science and Technology Agency Rental Coordination Survey] that are used as regular foods. Finally, the final culture can be made edible without undergoing separation and extraction operations.

In addition, in order to read without adding operations such as separation and extraction,
The abundant carbohydrates and proteins contained in edible ingredients are transformed into substances that are easily absorbed by the living body, and the honorable ingredients remain intact.

Grains include brown rice, wood such as white wood, raw buckwheat, buckwheat flour,
Corn, millet, oat, oat, etc. are all used, and rice and buckwheat flour in each form are preferably used.

Potatoes include sweet potatoes, potatoes, taro,
Use Japanese yam etc., preferably 1 sweet potato.

As the beans, soybeans, defatted soybeans, soybean products such as soybean flour, etc. are used, and soybeans are preferably used.

Further, this edible medium may contain only cereals or potatoes and beans, but glucose, magnesium sulfate (for food additives), and potassium phosphate (for food additives) may also be added. However, it does not contain anything other than Machiwa ingredients.

A basidiomycete of the genus Cyrilium? By culturing using this edible medium, a fermented food for use in non-exploited EJAK can be obtained.

This fermented food has all the properties of the first order.

(11 β-D-glucan awaiting pharmacological effects such as anticancer effect) Contains 0.05 to 10p/100f-powder in the dry powder state. This β-D-glucan has β-(1
, 5)-〇-glucan, and the average molecule 1 is about 1,080,000.

(2) Saponin with pharmacological effects such as anti-complement activation and antioxidant effects in dry powder form from 0.05 to 31/10
(M'- Contains powder.

(31 Ergosterol, which has pharmacological effects such as diuretic effect!li, 1.0 to 100.OhQ /
100V - Contains powder.

The fermented product used in the present invention can be produced, for example, by the following procedure.

Cereal Mayu, which contains a large amount of carbohydrates, is a medium composed only of edible ingredients, including a carbon source consisting of at least one tuber, and a nitrogen source consisting of beans containing a large amount of protein.
A liquid culture of basidiomycete mycelium of the genus C. chinensis is carried out.

The culture conditions are 31-45G above the culture temperature and 1! liquid pHt
-2.5 to 5.5. The aeration rate per culture pond 1 is 0.5 to 2.
By setting O2/order to 0.5 to 20 inoculated pots, culture can be carried out in 4 to 15 days. The fermentation product thus obtained is obtained by separating the edible medium, mycelium, and mycelium products from the culture solution after the cultivation is completed.

It can be used as a fermented food used in the present invention. Mayu can also be processed through dehydration to create a fermented food that contains as many useful ingredients as possible.

The appropriate intake amount of the food for people with liver dysfunction of the present invention is influenced by age, individual differences, symptoms, etc. Depending on the case, the appropriate amount may be outside the range below. When suspended in hot water, milk, etc., take 2 powders per day.
0 to 300 fi, 5 to 1011 liters of water per 12 powders, suspended in hot water, milk, etc., divided into 1 to 3 times 6
It is recommended to drink it for more than a month.

(Effect of the invention) The food for people with liver dysfunction of the present invention has at least β-D-/
It contains three types of active ingredients with pharmacological effects: lucan, saponin, and ergosterol, and provides at least the same level of pathological improvement effect as expected from the fruiting bodies of the basidiomycete of the genus Stone. Also, basidiomycetes and edible media provide proteins.

It also contains various nutritional components such as carbohydrates in a well-balanced manner, making it highly useful as a food for the sick. In addition, it is suitable as a "special nutritional food" in addition to its ability to improve pathological conditions.

From a medical and nutritional point of view, it is recommended not only for patients who require special nutritional support or for the purpose of improving disease conditions, but also for the prevention of liver dysfunction such as hepatitis. It can be used for the purposes of maintaining health and improving physical strength, and can be used by a wide range of people. Although the fruiting body of C. chinensis and its extract have a strong bitter taste peculiar to M. chinensis, 1 it cannot be used as a normal food. It has a certain bitterness and retains the flavor of the food material, which is a medium component, without impairing it, so it is suitable as a food. 7t. When the fermented food used in the present invention is obtained in liquid culture, the manufacturing conditions can be kept constant;
Stable quality of active ingredients and nutritional ingredients with pharmacological effects can be obtained.

(Examples, Test Examples) Examples are shown below, but the present invention is not limited to these Examples.

As shown in Test Example 1.2, the present invention is obtained by culturing the mycelium of a basidiomycete of the genus Hemorrhoids using a medium containing grains or potatoes and beans as constituents. The proteins and carbohydrates contained in fermented foods used for
The component cereal grains are broken down into free amino acids and monosaccharides that are easier to digest and absorb than when potatoes, beans, and basidiomycetes of the genus Ganoderma are simply mixed, resulting in a decline in digestive ability. It can be seen that this is a fc* product suitable for sick people.

EXAMPLE Test Using a fermenter, culturing of the Mycobacterium mycelium was carried out in the following manner.

In a test fermenter with a capacity of 100 g, the edible medium components shown in the table were dissolved in 40 t of water, the pH was adjusted to 4.0, and the
It was sterilized at 20C and 2 atm for 30 minutes. Nine species of mycelial culture 1.6 obtained by shaking culture for 32'C14 days in an edible medium with the same composition.
A friend who has been inoculated with T. A viscous culture was obtained by maintaining the culture temperature at 32'CK and culturing with aeration (404/l1il) and agitation (200r, pom, ) for 7 days. This was freeze-dried and 1.5 kg of powder was obtained. This powder.

It has a mild flavor and a slight bitterness, making it suitable as a food, and it also contains the nutritional and active ingredients shown in Table 2. However, β-D-glucan and soybean saponin were determined by gas chromatography, and ergosterol was determined by high performance liquid chromatography using 50 g of this powder! If you drink a viscous liquid made by adding 3oo- of hot water (aoC) to the water, 3 times/day for 6 months.

A friend who has found revenge in patients with chronic hepatitis. Table 3 shows the patient's serum transaminase levels after drinking and drinking.

As shown in Table 3, a decrease was observed in both CPT and GOT.

Table 1 Table I @ Table 2 Experimental Example 1 We decided to investigate how proteins among the 633 components of the culture medium have changed by conducting a one-line culture of Basidizono of the genus Manjotake.

The following trial 1ili1 was conducted.

Soybeans (whole grains) 50 f'i500tRt Place in Erlenmeyer flask and add glucose 3.056. Kend Magnesium 0.1%,! 77g-force! J U A O, 19
bf) Add 3O-t of mK (Dffl solution) and sterilize at 120C and 2 atm for 20 minutes.

Without adding the strands, the mixture was allowed to stand at 32C for 7 days, and then centrifuged to obtain only the supernatant. This is designated as Sample I.

Similarly, put 302 soybeans (whole grains) into a 500-Erlenmeyer flask, and put 5. Os, aM7/nesium 0.
1%, potassium phosphate-0.1% solution 30wt
In addition to step 1, sterilize at 120C and 2 atm for 20 minutes. After cooling to 32G, add 8% (v/v) of the inoculum of the ``Salthorn bamboo culture'' which was mixed with medium components using a water tip. 32
After static culture at C for 7 days, only the supernatant was obtained by centrifugation. This is designated as sample (■).

When the free amino acids in the solutions of Sample I and Sample (2) were measured, it was found that Sample (2) had more amino acids than each other. In other words, it was found that by mixing the entire body of the Cinnamon mushroom box, the proteins in the culture medium were decomposed into free amino acids that were easily digested and absorbed.

The results are shown in Table 4.

Test Example 2 In order to investigate how carbohydrates among the refeeding components, which are medium components, have changed by carrying out a monotonic culture of Basidiomyces spp.

The following tests were conducted.

Starch 5%, corn steep liquor 2es, magnesium sulfate 0.1ts, potassium phosphate 0.1%
Create a medium with the composition of 6Affi and adjust the pH to 4.0.
After that, put 100 d of this solution into a 500 d slope flask,
Sterilize at 120C 2 atm for 20 minutes. After cooling to 32C,
The medium components were removed by washing with water, and then 4% (v/v) of manneum/bamboo cultured mycelium was added as an inoculum, and cultured with shaking at 32c for 8 days.

The liquid before the start of culture and after the end of culture is centrifuged to obtain only the supernatant, which is used as sample ① and sample ②.

When the amount of monosaccharide and oligokinut in the #liquid was measured for sample (■) and sample (2), it was found that the amount of monosaccharide was increased in sample IV compared to sample (■). In other words, by mixing in the mycelium of Cinnamon mycelia.

A friend who knows that the polysaccharides in the culture are broken down into monosaccharides that are easily absorbed and decomposed. The results are shown in Table 5.

Claims (1)

[Claims] Consisting of basidiomycetes belonging to the genus Hemorrhoids, grains or tubers, and beans, β-D-glucan, saponin,
A food for people with liver dysfunction, characterized in that a fermented food containing ergosterol is used for people with liver dysfunction.