JPS62232312A - Nass formation of eggplant adventive germ - Google Patents
Nass formation of eggplant adventive germInfo
- Publication number
- JPS62232312A JPS62232312A JP61071204A JP7120486A JPS62232312A JP S62232312 A JPS62232312 A JP S62232312A JP 61071204 A JP61071204 A JP 61071204A JP 7120486 A JP7120486 A JP 7120486A JP S62232312 A JPS62232312 A JP S62232312A
- Authority
- JP
- Japan
- Prior art keywords
- callus
- somatic embryos
- eggplant
- formation
- adventive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000061458 Solanum melongena Species 0.000 title claims description 9
- 235000002597 Solanum melongena Nutrition 0.000 title claims description 8
- 230000015572 biosynthetic process Effects 0.000 title description 3
- 230000000392 somatic effect Effects 0.000 claims description 23
- 210000002257 embryonic structure Anatomy 0.000 claims description 21
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 19
- 230000001939 inductive effect Effects 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000000034 method Methods 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 210000001161 mammalian embryo Anatomy 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000012136 culture method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 241000208173 Apiaceae Species 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 101100017029 Hordeum vulgare HINA gene Proteins 0.000 description 1
- 241000218201 Ranunculaceae Species 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明はナスのクローン苗の大量、高速増殖法に関する
ものであり、農業分野において完成した品種を採種する
に先立って速かに大量に栄養繁殖する目的に、および優
れた個体を栄養繁殖し、品種として直接利用する等の目
的のために用いられる。[Detailed Description of the Invention] [Field of Industrial Application] The present invention relates to a method for mass-propagation and high-speed propagation of cloned eggplant seedlings. It is used for purposes such as breeding, vegetative propagation of superior individuals, and direct use as varieties.
採種に先立って完成した品種を速かに大量に増殖すると
か、あるいは優れた個体を栄＃繁殖して品種として直接
利用する目的で苗を栄養繁殖する方法としては、さし末
法および組織培養を用いる方法がある。The cutting method and tissue culture are methods of vegetatively propagating seedlings for the purpose of rapidly propagating completed varieties in large quantities prior to seed collection, or for breeding excellent individuals and directly using them as varieties. There is a method to use.
組織培養法には成長点培養法と不定胚を誘導する方法が
ある。Tissue culture methods include meristem culture methods and methods for inducing somatic embryos.
組織培養法によって苗を大量に増殖し、それを直接利用
する場合には、不定胚を形成させる方法の方が、成長点
という個体あたりに極く限定された数の組織しか利用し
ない成長点培養法に比較して明らかに優れている。If you want to propagate a large number of seedlings using tissue culture and use them directly, it is better to form somatic embryos using meristem culture, which uses only a very limited number of tissues per individual called meristem. clearly superior to the law.
セリ科、キンポウゲ科、ウコギ科など１部の植物種では
このような一旦カルスを誘導しそれから不定胚を誘導す
る技術はあるが、その不定胚は未だ安定性に乏しいもの
でしか無かった。For some plant species, such as Apiaceae, Ranunculaceae, and Araliaceae, there is a technique to induce callus and then to induce somatic embryos, but the somatic embryos still lack stability.
ナス科植物についても、不定胚の形成を誘導する報告は
みられるものの（文献： ＭＡＴＳＵＯＫＡ、ＨＩＮＡ
ＴＡ、Ｊａｕｒｎａｌ ｏｆ Ｅｘｐｅｒｉｍｅｎｔｅ
ｄ Ｂｏｔａｎｙ、３０（１１６）：３６３〜３７０（
１９７９）、ＭＡＴＳＵＯＫＡ、ＨＩＮＡＴＡ Ｊａｐ
ａｎ、Ｊ、Ｂｒｅｅｄ、。Although there are reports that solanaceous plants induce the formation of somatic embryos (References: MATSUOKA, HINA
TA, Journal of Experiment
d Botany, 30(116):363-370(
1979), MATSUOKA, HINATA Jap
an, J, Breed,.
３３ （３） ： ３０３〜３０９ （１９８３） ）
、安定して大量の不定胚を形成させるには到っていない
。33 (3): 303-309 (1983))
However, it has not been possible to stably form a large number of somatic embryos.
このようにナスにおいて不定胚を能率よく大量に誘導さ
せる技術は未だ完成されておらず本発明の目的はこの点
即ち、効率よく大量に不定胚を形成させる方法の開発に
ある。As described above, the technology for efficiently inducing somatic embryos in large numbers in eggplants has not yet been perfected, and the purpose of the present invention is to develop a method for efficiently forming somatic embryos in large quantities.
本発明はナスにおける不定胚の形成を能率よく大量に行
わせるために、培養方法を新しく開発したものである。The present invention is a newly developed culture method for efficiently forming somatic embryos in large quantities in eggplants.
本発明を更に具体的に説明すると、ナス植物体を材料と
しカルスを誘導し、そのカルスから１旦不定胚またはグ
リンスポットを形成させ、さらに再びこれを材料としカ
ルスを形成させる。そして、このカルスをもとに再度不
定胚を誘導することにより、効率よい大量の不定胚の形
成を果たすものである。To explain the present invention more specifically, a callus is induced using an eggplant plant as a material, a somatic embryo or a green spot is formed from the callus, and then a callus is formed using this as a material again. Then, by inducing somatic embryos again based on this callus, a large number of somatic embryos can be efficiently formed.
本発明に言う不定胚とは、培養細胞中に出現する長さ０
．１〜５ｍｍの幼根、子葉、など胚と類似の器官を備え
た構造体を指し、グリーン・スポットとは、培養細胞中
に出現する直径１〜５ＩＩＩｍ程度の緑色のコンパクト
な細胞塊を指す。またカルスとは、ナス細胞が分化した
構造体を形成せずに活発に増殖した細胞塊を指す。The somatic embryo referred to in the present invention is a 0-length embryo that appears in cultured cells.
．． A green spot refers to a structure with organs similar to an embryo, such as a radicle and cotyledons measuring 1 to 5 mm, and a green spot refers to a compact green cell mass with a diameter of about 1 to 5 III m that appears in cultured cells. In addition, callus refers to a cell mass in which eggplant cells actively proliferate without forming a differentiated structure.
ナス展開葉の中肋を滅菌し、ＮＡＡを１０ ｐｐｍ添加
した寒天培地に植え込んだ。The midrib of the expanded eggplant leaf was sterilized and planted on an agar medium supplemented with 10 ppm of NAA.
基本培地としては表に示すＭＳ修正培地（検問、日向１
９７９）を用いた。As a basic medium, the MS modified medium shown in the table (Inquiry, Hinata 1
979) was used.
これを２６℃暗所で４０日間培養後、中肋に誘導され、
増殖したカルス５０ｍｇをＮＡＡ４〜８ｐｐｍ添加した
液体培地１０ ｍｌに移植し、明所で１ケ月間８０ｒｐ
ｍで填盪培養をおこなった。After culturing this in the dark at 26°C for 40 days, it was induced into the midrib,
50 mg of proliferated callus was transplanted into 10 ml of liquid medium supplemented with 4-8 ppm of NAA, and incubated at 80 rpm in the light for 1 month.
Packaging culture was performed at m.
この培養によりカルスからいくつかの不定胚が形成され
た。This culture resulted in the formation of several somatic embryos from the callus.
比重差を利用してカルスからこれら形成された不定胚を
分けとり、これをＮＡＡ４〜ｓ ｐｐｍ添加した液体培
地１００ｍ１に移植し振盪培養をおこなったところ、不
定胚由来のカルスが形成された。These formed somatic embryos were separated from the callus using the difference in specific gravity, and when they were transplanted into 100 ml of a liquid medium supplemented with 4 to sppm of NAA and cultured with shaking, callus derived from somatic embryos was formed.
２週間後カルスをホルモンフリーの培地に移植し、振盪
培養を行った。Two weeks later, the callus was transplanted to a hormone-free medium and cultured with shaking.
１週間後カルスから多くの不定胚が形成されたのでこれ
らを分けとり、ホルモンフリー寒天培地に置床して植物
体へと成育させた。残りのカルスおよび不定胚をＮＡＡ
４〜８ｐｐｍ添加した液体培地に移植し２週間振盪培
養をおこなったのち、ホルモンフリー培地に移植し振盪
培養した。One week later, many somatic embryos were formed from the callus, and these were separated and placed on a hormone-free agar medium to grow into plants. NAA the remaining callus and somatic embryos
After transplanting to a liquid medium supplemented with 4 to 8 ppm and culturing with shaking for 2 weeks, the cells were transplanted to a hormone-free medium and cultured with shaking.
１ケ月後、カルスから多くの不定胚が形成されたのでこ
れらを分けとり、ホルモンフリー寒天培地に置床して植
物体へと成育させた。One month later, many somatic embryos were formed from the callus, and these were separated and placed on a hormone-free agar medium to grow into plants.
本発明により開発された不定胚の大量形成法によりナス
の種苗が大量に能率よく生産できるようになった。The method for mass-producing somatic embryos developed by the present invention has made it possible to efficiently produce large quantities of eggplant seedlings.
Claims (1)
導した不定胚またはグリンスポットを培養し、これから
再度カルスを誘導し、増殖したカルスから不定胚を誘導
せしめる不定胚の大量形成法。A method for producing a large amount of somatic embryos by inducing callus from the stems and leaves of eggplant, culturing somatic embryos or green spots derived from the callus, inducing callus again from the callus, and inducing somatic embryos from the multiplied callus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61071204A JPS62232312A (en) | 1986-03-31 | 1986-03-31 | Nass formation of eggplant adventive germ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61071204A JPS62232312A (en) | 1986-03-31 | 1986-03-31 | Nass formation of eggplant adventive germ |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62232312A true JPS62232312A (en) | 1987-10-12 |
Family
ID=13453912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61071204A Pending JPS62232312A (en) | 1986-03-31 | 1986-03-31 | Nass formation of eggplant adventive germ |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62232312A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997012512A3 (en) * | 1995-10-04 | 1997-05-22 | Calgene Inc | Transformation of cotton plants |
| CN112586346A (en) * | 2020-12-09 | 2021-04-02 | 华中农业大学 | Tree eggplant, and cultivation method, rapid propagation method and application thereof |
-
1986
- 1986-03-31 JP JP61071204A patent/JPS62232312A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997012512A3 (en) * | 1995-10-04 | 1997-05-22 | Calgene Inc | Transformation of cotton plants |
| US5846797A (en) * | 1995-10-04 | 1998-12-08 | Calgene, Inc. | Cotton transformation |
| CN112586346A (en) * | 2020-12-09 | 2021-04-02 | 华中农业大学 | Tree eggplant, and cultivation method, rapid propagation method and application thereof |
| CN112586346B (en) * | 2020-12-09 | 2022-03-01 | 华中农业大学 | Tree eggplant, and cultivation method, rapid propagation method and application thereof |
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