JPS62192661A - Method for measuring crp by immune nephelometry - Google Patents
Method for measuring crp by immune nephelometryInfo
- Publication number
- JPS62192661A JPS62192661A JP3386486A JP3386486A JPS62192661A JP S62192661 A JPS62192661 A JP S62192661A JP 3386486 A JP3386486 A JP 3386486A JP 3386486 A JP3386486 A JP 3386486A JP S62192661 A JPS62192661 A JP S62192661A
- Authority
- JP
- Japan
- Prior art keywords
- crp
- antibody
- monoclonal antibody
- measurement
- measuring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000004848 nephelometry Methods 0.000 title 1
- 238000006243 chemical reaction Methods 0.000 claims abstract description 12
- 239000000427 antigen Substances 0.000 claims abstract description 4
- 102000036639 antigens Human genes 0.000 claims abstract description 4
- 108091007433 antigens Proteins 0.000 claims abstract description 4
- 239000007791 liquid phase Substances 0.000 claims abstract 2
- 230000000890 antigenic effect Effects 0.000 claims description 4
- 238000000691 measurement method Methods 0.000 claims description 3
- 102100032752 C-reactive protein Human genes 0.000 abstract description 23
- 238000005259 measurement Methods 0.000 abstract description 9
- 108010074051 C-Reactive Protein Proteins 0.000 abstract description 5
- 230000006866 deterioration Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 9
- 238000002835 absorbance Methods 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 210000000628 antibody-producing cell Anatomy 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 102000018297 Immunoglobulin subtype Human genes 0.000 description 1
- 108050007411 Immunoglobulin subtype Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000012506 Sephacryl® Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000000951 immunodiffusion Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 210000005265 lung cell Anatomy 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明はＣＲＰ即ちＣ反応性蛋白の測定法に係り、殊に
免疫比濁法によるその測定法に係る。DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a method for measuring CRP or C-reactive protein, and in particular to a method for measuring it by immunoturbidimetry.
ＣＲＰは、周知のように、炎症や組織壊死が生ずる場合
に血中に出現する特性を有している蛋白であるが、正常
人の血清中にも微量のＣＲＰが存在していることが明ら
かとなり、その機能が解明されつつある。As is well known, CRP is a protein that has the characteristic of appearing in the blood when inflammation or tissue necrosis occurs, but it is clear that trace amounts of CRP also exist in the serum of normal people. Its function is now being elucidated.
従って、本発明による測定法は上記の炎症や感染症等の
臨床診断における検査法の一つとして利用することがで
きる。Therefore, the measuring method according to the present invention can be used as one of the testing methods for clinical diagnosis of the above-mentioned inflammation, infection, etc.
（従来の技術）
従来におけるＣＲＰの定量法としては毛細管法、ラテッ
クス凝集法、−元免疫拡散法及び免疫比濁法がある。(Prior Art) Conventional methods for quantifying CRP include a capillary tube method, a latex agglutination method, an immunodiffusion method, and an immunoturbidimetric method.
これらの従来法の内で免疫比濁法においては抗体として
ポリクローナル抗体が採用されている。Among these conventional methods, polyclonal antibodies are employed as antibodies in immunoturbidimetry.
（発明が解決しようとする問題点）
抗ＣＲＰモノクローナル抗体を用い高感度な且つ特異性
の高い測定系を確立するためには、ポリクローナル抗体
を親和性クロマトグラフィー等により充分に精製する必
要がある。又、ポリクローナル抗体は免疫動物の個体差
等により異なるものとなり、従って常に一定の品質乃至
性質を有する抗体を調製することが困難であると謂う問
題を有していた。(Problems to be Solved by the Invention) In order to establish a highly sensitive and highly specific measurement system using an anti-CRP monoclonal antibody, it is necessary to sufficiently purify the polyclonal antibody by affinity chromatography or the like. Furthermore, polyclonal antibodies vary due to individual differences in the immunized animal, and therefore there is a problem in that it is difficult to always prepare antibodies with constant quality or properties.
（発明の目的）
従って、本発明の目的は、特異性が高く且つ安定なＣＲ
Ｐの測定法を提供することにある。(Object of the invention) Therefore, the object of the present invention is to provide highly specific and stable CR.
The purpose of this invention is to provide a method for measuring P.
（目的を達成するための手段及び作用）本発明方法によ
れば、上記の目的は、免疫比濁法によりＣＲＰを測定す
るに際して、抗体として抗ＣＲＰモノクローナル抗体を
用いることを特徴とする、免疫比濁法によるＣＲＰの測
定法により達成することができる。即ち、本発明方法に
おいては、測定されるべき抗原（ＣＲＰ）と反応する抗
体として抗ＣＲＰモノクローナル抗体が使用されるため
に、ポリクローナル抗体を使用する場合と比較して反応
における特異性が高く、従って試料（検体）中にたとえ
構造の近似した物質が存在しても測定精度の低下を来す
ことがない。更に、モノクローナル抗体は、その産生細
胞株から常に一定の性質を有するものを作成できるので
、その安定な供給が可能であり、従って使用される抗体
に依存して測定精度が変化するのを阻止することができ
る。(Means and effects for achieving the object) According to the method of the present invention, the above object is to use an anti-CRP monoclonal antibody as an antibody when measuring CRP by immunoturbidimetry. This can be achieved by measuring CRP using a turbidity method. That is, in the method of the present invention, since an anti-CRP monoclonal antibody is used as an antibody that reacts with the antigen to be measured (CRP), the specificity of the reaction is higher than when using a polyclonal antibody. Even if a substance with a similar structure exists in the sample (specimen), the measurement accuracy will not deteriorate. Furthermore, since monoclonal antibodies can always be produced with constant properties from the cell line that produces them, a stable supply is possible, thus preventing measurement accuracy from varying depending on the antibody used. be able to.
本発明方法の実施に際しては、ゲル内及び液相中での抗
ｆＭ （ＣＲＰ）との反応において濁度を形成する抗Ｃ
ＲＰモノクローナル抗体を用いるのが好ましい。更に、
複数種類の抗ＣＲＰモノクローナル抗体を用いる場合に
は、認識する抗原決定基が互いに異なるものを用いるの
が好ましい。When carrying out the method of the present invention, anti-C
Preferably, RP monoclonal antibodies are used. Furthermore,
When using multiple types of anti-CRP monoclonal antibodies, it is preferable to use antibodies that recognize different antigenic determinants.
（発明の効果）
免疫比濁法によりＣＲＰを測定するに際して、本発明方
法によれば、抗体として抗ＣＲＰモノクローナル抗体が
用いられるので特異性及び測定感度を向上させることが
できる。又、モノクローナル抗体であるために、常に一
定の性質乃至品質を有するものを入手使用することがで
き、従って。(Effects of the Invention) When measuring CRP by immunoturbidimetry, according to the method of the present invention, an anti-CRP monoclonal antibody is used as the antibody, so that specificity and measurement sensitivity can be improved. Furthermore, since it is a monoclonal antibody, it can always be used with constant properties and quality.
測定結果の信頼性が向上する。The reliability of measurement results is improved.
（実施例）
ａ）抗ＣＲＰモノクローナル抗体産生細胞の作製患者血
清を原料とし、これを硫安分画、ＤＥＡＥイオン交換ク
ロマトグラフィー、Ｃａ”＋沈澱、セファクリルＳ −
３００ゲル濾過により精製したＣＲＰをＢＡＬＢ／Ｃ系
マウスに免疫し、その肺細胞をマウス骨髄腫細胞（Ｐ２
Ｏ３）と融合し、限界稀釈法により５種類のモノクロー
ナル抗体産生細胞株（ＣＲＢ１７、ＣＲＢ１８、Ｃ１１
Ｂ１９、ＣＲＢ２０、ＣＲＢ２３）を得た。(Example) a) Preparation of anti-CRP monoclonal antibody-producing cells Using patient serum as a raw material, it was subjected to ammonium sulfate fractionation, DEAE ion exchange chromatography, Ca"+ precipitation, and sephacryl S-
BALB/C mice were immunized with CRP purified by 300 gel filtration, and the lung cells were injected into mouse myeloma cells (P2
03) and five types of monoclonal antibody producing cell lines (CRB17, CRB18, C11) by limiting dilution method.
B19, CRB20, CRB23) were obtained.
ｂ）抗ＣＲＰモノクローナル抗体の精製マウス腹水中で
産生させたモノクローナル抗体を硫安分画、ＤＥＡＥイ
オン交換クロマトグラフィー、プロティンＡセファロー
スＣＬ−４Ｂにより精製したものを実験に使用した。尚
、抗体産生細胞を無血清培地で培養した培養上清を濃縮
し、５０％飽和硫安で分画してモノクローナル抗体を回
収し、これを実験に供することもできる。b) Purification of anti-CRP monoclonal antibody A monoclonal antibody produced in mouse ascites was purified by ammonium sulfate fractionation, DEAE ion exchange chromatography, and Protein A Sepharose CL-4B and used in the experiment. Incidentally, the culture supernatant obtained by culturing antibody-producing cells in a serum-free medium can also be concentrated and fractionated with 50% saturated ammonium sulfate to recover monoclonal antibodies, which can also be used in experiments.
Ｃ）抗ＣＲＰモノクローナル抗体のサブクラス及び抗原
決定基の解析
各イムノグロブリンサブタイプに対する抗血清を用いて
ゲル内沈降反応で調べた結果、ＣＩ（８１７がＩｇＧ１
であり、Ｃ１１Ｂ１９及びＣＲＢ２３が躇Ｇ３であり、
ＣＲＢ１９及びＣＩ’ｔＢ２０がＩｇＧ２ｂであった。C) Analysis of subclasses and antigenic determinants of anti-CRP monoclonal antibodies As a result of in-gel precipitation analysis using antisera against each immunoglobulin subtype, CI (817 was IgG1
, C11B19 and CRB23 are hesitation G3,
CRB19 and CI'tB20 were IgG2b.
又、これらのモノクローナル抗体の抗原決定基について
ゲル内沈降反応及びＥＩＡにて検討を行った結果、これ
らの５種類のモノクローナル抗体はＣＲＢ１７とＣＲＢ
２０の２種類からなるグループと、ＣＲＢ１８とＣＲ口
１９とＣＲＢ２３の３種類からなるグループとに分かれ
ることが判明した。このことは、各グループがＣＲＰ分
子上の異なる抗原決定部位を認識して結合するものであ
ることを示唆している。Furthermore, as a result of examining the antigenic determinants of these monoclonal antibodies using in-gel precipitation reaction and EIA, it was found that these five types of monoclonal antibodies have CRB17 and CRB.
It was found that there are two groups: a group consisting of two types of 20, and a group consisting of three types: CRB18, CR mouth 19, and CRB23. This suggests that each group recognizes and binds to a different antigen-determining site on the CRP molecule.
Ｃ）測定方法
ｉ）上記のｂ）項に記載の方法により得た抗ＣＲＰモノ
クローナル抗体を、 ５％ ＰＩＥＧ ６０００ 及び
０．２％Ｔｗｅｅｎ ２０を含有する０、０２Ｍ ＨＥ
ＰＥＳ緩衝液（ｐＨ７，４，０，ＩＭ ＮａＣ１含有）
で１０倍に稀釈して反応液とする。この反応液１ ｍｌ
に検体２０μｍを添加し３７℃で３０分間インキュベー
トした後に、分光光度計により３４０ ｎｍで吸光度を
測定した。一方、上記と同様の反応液であって、但し抗
体を含有しない系を別途に準備し、この系につき上記と
同様に処理した上で吸光度を測定した。両眼光度のＯ，
Ｄ、差を反応生成物の量とする。C) Measurement method i) The anti-CRP monoclonal antibody obtained by the method described in b) above was added to 0.02M HE containing 5% PIEG 6000 and 0.2% Tween 20.
PES buffer (pH 7, 4, 0, containing IM NaCl)
Dilute the solution 10 times with water to prepare a reaction solution. 1 ml of this reaction solution
After adding 20 μm of specimen to the solution and incubating at 37° C. for 30 minutes, absorbance was measured at 340 nm using a spectrophotometer. On the other hand, a system using the same reaction solution as above but containing no antibody was separately prepared, and this system was treated in the same manner as above and the absorbance was measured. Binocular luminosity O,
D. Let the difference be the amount of reaction product.
ｉｉ） ５％ＰＥＧ ６０００．０．２％Ｔｗｅｅｎ
２０及び０．１ＭＮａＣ１を含有する０、０２Ｍ ＨＥ
ＰＥＳ緩衝液（ｐＨ７，４）である反応液Ｉ （５００
μｍ）に検体１０μｍを添加し３７℃で５分間ブレイン
キュベートし、上記のｂ）項に記載の方法により得た抗
ＣＲＰモノクローナル抗体を含有するＰＢＳ （ｐＨ７
，４）である反応液ＩＩ （５０μｍ）を添加して更に
５分間インキュベートした後に１分光光度計により３４
０ ｎｍで吸光度を測定した。一方、上記と同様にして
、但し反応液Ｉ工として抗体を含有しないものを使用し
た系について吸光度を測定した。両級光度の０、Ｄ、差
を反応生成物の量とする。ii) 5% PEG 6000.0.2% Tween
0,02M HE containing 20 and 0.1M NaCl
Reaction solution I (500
10 μm of the sample was added to 10 μm of the sample, incubated at 37°C for 5 minutes, and PBS (pH 7
, 4) was added and incubated for an additional 5 minutes.
Absorbance was measured at 0 nm. On the other hand, the absorbance was measured in the same manner as above except that a reaction solution containing no antibody was used as the reaction solution I. The 0, D, difference between the two luminous intensities is taken as the amount of reaction product.
ｅ）結果
ｄ−ｉ） の゛ −ｊ法による− 結果上記のｂ）項
に記載の方法により精製された５種類の抗ＣＲＰモノク
ローナル抗体をそれぞれ単独で用い且つ標準血清（ＣＲ
Ｐ ８度が１．０．５．０及び］、Ｏ，Ｏｍｇ／ｄｌの
もの）を用いて濁度形成を調べた結果は、第１図のグラ
フに示される通りであり、モノクローナル抗体の種類に
応じて差はあるが、何れのモノクローナル抗体も濁度を
形成することが判明した。尚、このグラフから、ＣＲＢ
１７は全体的に、又ＣＲＢ２３は高濃度での濁度形成に
おいて良好なことが判る。e) Results d-i) ゛ -j method-Results Each of the five anti-CRP monoclonal antibodies purified by the method described in b) above was used alone and standard serum (CR
The results of investigating turbidity formation using P 8 degrees 1.0. It was found that all monoclonal antibodies formed turbidity, although there were differences depending on the conditions. Furthermore, from this graph, CRB
It can be seen that 17 is good overall and CRB23 is good in turbidity formation at high concentrations.
従って、モノクローナル抗体ＣＲＢ１７とＣＲＢ２３と
を用い且つこれらの混合比率を１：１．２：ｌ及び１：
２で変化させ、標準血清（ＣＲＰ濃度が１．０．５．０
及び１０．０ ｍｇ／ｄｉのもの）を用いて濁度形成を
調べた結果は、第２Ａ、２Ｂ及び２Ｃ図のグラフに示さ
れる通りであり、 ＣＲＢ１７を多くすれば低ａ度での
感度が向上し、一方ＣＲＢ２３を多くすれば高濃度での
濁度形成が良好となること並びに両者の混合比率を適当
に設定することにより、例えば１：１に設定することに
より （第２Ａ図）、検量線の直線化が可能なることが
判明した。Therefore, monoclonal antibodies CRB17 and CRB23 were used and their mixing ratios were 1:1.2:1 and 1:1.
2 and standard serum (CRP concentration 1.0.5.0
The results of investigating turbidity formation using CRB17 and 10.0 mg/di are shown in the graphs in Figures 2A, 2B, and 2C. On the other hand, increasing CRB23 improves turbidity formation at high concentrations, and by appropriately setting the mixing ratio of both, for example, 1:1 (Figure 2A), calibration can be achieved. It turns out that it is possible to straighten lines.
ｄ−ｉｉ のリ 法による 験結果上記の第ｄ−
ｉｉ）項に記載の測定方法に従い、検体として標準血清
（ＣＲＰ濃度がそれぞれ１．０．５．０及び１０．０
ｍｇ／ｄｌのもの）を用い且つ反応液ＩＩに含有される
モノクローナル抗体とじてＣＲＢ１７とＣＲＢ２３のｌ
：ｌ混合物を用いて濁度形成を３４０ ｎｍで調べた結
果は、第３図のグラフに示される通りであった。The experimental results of d-ii using the method d-ii above
According to the measurement method described in section ii), standard serum (with CRP concentrations of 1.0, 5.0 and 10.0, respectively) was used as the specimen.
of CRB17 and CRB23 as monoclonal antibodies contained in reaction solution II.
The results of examining the turbidity formation at 340 nm using the :1 mixture were as shown in the graph of FIG.
このグラフにおけるＣＲＰ濃度と濁度との関係は極めて
直線的なものであり、従ってこれは検量線として用いる
のに好適なことが判る。The relationship between CRP concentration and turbidity in this graph is extremely linear, and therefore it can be seen that this is suitable for use as a calibration curve.
第１図は、各種のモノクローナル抗体を用いて１本発明
方法によりＣＲＰを測定する場合のＣＲＰ濃度と吸光度
との関係を示すグラフであり、第２Ａ図、２８図及び２
０図は２種類のモノクローナル抗体即ちＣＲＢ１７及び
ＣＲＢ２３を且つこれらの混合比率を１：１．２：１及
び１：２で用いて、本発明方法によりＣＲＰを測定した
場合のＣＲＰ濃度と吸光度との関係を示すグラフであり
、第３図はモノクローナル抗体ＣＲＢ１７とＣＲＢ２３
の１：１混合物を抗体として本発明方法によりＣＲＰを
測定した場合のＣＲＰｆ、３度と吸光度との関係を示す
グラフであって、検量線として好適なものを示すグラフ
である。
第１図
ＣＲＰＪＪ５ （ｍｇ／ｄｌ）
Φ−・ＣＲＢ１７
ｏ−ｏ（Ｒ日１８
Δ−ΔＣＲ日１９
閣日間９ＲＢ２０
ム一ムＣＲＥ３２３FIG. 1 is a graph showing the relationship between CRP concentration and absorbance when CRP is measured by the method of the present invention using various monoclonal antibodies, and FIGS.
Figure 0 shows the relationship between CRP concentration and absorbance when CRP was measured by the method of the present invention using two types of monoclonal antibodies, namely CRB17 and CRB23, at a mixing ratio of 1:1.2:1 and 1:2. FIG. 3 is a graph showing the relationship between monoclonal antibodies CRB17 and CRB23.
2 is a graph showing the relationship between CRPf, 3 degrees and absorbance when CRP is measured by the method of the present invention using a 1:1 mixture of the antibodies as an antibody, and is a graph showing a suitable calibration curve. Figure 1 CRPJJ5 (mg/dl) Φ-・CRB17 o-o (R day 18 Δ-ΔCR day 19 Cabinet day 9RB20 Muichimu CRE323
Claims (3)
体として抗ＣＲＰモノクローナル抗体を用いることを特
徴とする、免疫比濁法によるＣＲＰの測定法。(1) A method for measuring CRP by immunoturbidimetry, characterized in that an anti-CRP monoclonal antibody is used as an antibody when measuring CRP by immunoturbidimetry.
を形成する抗ＣＲＰモノクローナル抗体を用いることを
特徴とする、特許請求の範囲第１項に記載の免疫比濁法
によるＣＲＰの測定法。(2) Determination of CRP by immunoturbidimetry according to claim 1, which uses an anti-CRP monoclonal antibody that forms turbidity upon reaction with an antigen in a gel and in a liquid phase. Measurement method.
モノクローナル抗体を用いることを特徴とする、特許請
求の範囲第１項に記載の免疫比濁法によるＣＲＰの測定
法。(3) Multiple types of anti-CRP that recognize different antigenic determinants
The method for measuring CRP by immunoturbidimetry according to claim 1, which uses a monoclonal antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61033864A JP2607363B2 (en) | 1986-02-20 | 1986-02-20 | CRP measurement by immunoturbidimetry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61033864A JP2607363B2 (en) | 1986-02-20 | 1986-02-20 | CRP measurement by immunoturbidimetry |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62192661A true JPS62192661A (en) | 1987-08-24 |
| JP2607363B2 JP2607363B2 (en) | 1997-05-07 |
Family
ID=12398365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61033864A Expired - Fee Related JP2607363B2 (en) | 1986-02-20 | 1986-02-20 | CRP measurement by immunoturbidimetry |
Country Status (1)
| Country | Link |
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| JP (1) | JP2607363B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6415656A (en) * | 1987-07-09 | 1989-01-19 | Nissui Seiyaku Co | Method for quantifying c reactive protein |
| JPH0264458A (en) * | 1988-08-31 | 1990-03-05 | Nippon Shoji Kk | Determination of serum apo b |
| JPH0264457A (en) * | 1988-08-31 | 1990-03-05 | Nippon Shoji Kk | Determination of serum apo a-i |
| US10852300B2 (en) | 2015-06-01 | 2020-12-01 | Tanaka Kikinzoku Kogyo K.K. | Immunochromatographic analyzer for Mycoplasma pneumoniae detection |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004045384A (en) | 2002-05-22 | 2004-02-12 | Matsushita Electric Ind Co Ltd | Immunological measurement method, immunological measuring apparatus, tray for measuring organism component antialbuminmonoclonal antibody, cell for producing the antialubminmonoclonal antibody, and albumin detection kit |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58211659A (en) * | 1982-06-03 | 1983-12-09 | Nitsusui Seiyaku Kk | Handy and quick assay of serum crp by immunological nephelometry |
| JPS60237363A (en) * | 1984-05-11 | 1985-11-26 | Wako Pure Chem Ind Ltd | Assay of immunoglobulin |
-
1986
- 1986-02-20 JP JP61033864A patent/JP2607363B2/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58211659A (en) * | 1982-06-03 | 1983-12-09 | Nitsusui Seiyaku Kk | Handy and quick assay of serum crp by immunological nephelometry |
| JPS60237363A (en) * | 1984-05-11 | 1985-11-26 | Wako Pure Chem Ind Ltd | Assay of immunoglobulin |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6415656A (en) * | 1987-07-09 | 1989-01-19 | Nissui Seiyaku Co | Method for quantifying c reactive protein |
| JPH0264458A (en) * | 1988-08-31 | 1990-03-05 | Nippon Shoji Kk | Determination of serum apo b |
| JPH0264457A (en) * | 1988-08-31 | 1990-03-05 | Nippon Shoji Kk | Determination of serum apo a-i |
| US10852300B2 (en) | 2015-06-01 | 2020-12-01 | Tanaka Kikinzoku Kogyo K.K. | Immunochromatographic analyzer for Mycoplasma pneumoniae detection |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2607363B2 (en) | 1997-05-07 |
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