JPS62183882A - Preparation of protein built-up film - Google Patents
Preparation of protein built-up filmInfo
- Publication number
- JPS62183882A JPS62183882A JP61025008A JP2500886A JPS62183882A JP S62183882 A JPS62183882 A JP S62183882A JP 61025008 A JP61025008 A JP 61025008A JP 2500886 A JP2500886 A JP 2500886A JP S62183882 A JPS62183882 A JP S62183882A
- Authority
- JP
- Japan
- Prior art keywords
- film
- protein
- substrate
- monomolecular film
- glucose oxidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 30
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 30
- 239000000758 substrate Substances 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 238000004519 manufacturing process Methods 0.000 claims description 13
- 238000009825 accumulation Methods 0.000 claims description 10
- 239000003960 organic solvent Substances 0.000 claims description 7
- 239000003431 cross linking reagent Substances 0.000 claims description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract description 26
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract description 16
- 235000011187 glycerol Nutrition 0.000 abstract description 13
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 abstract description 10
- 229940116332 glucose oxidase Drugs 0.000 abstract description 10
- 108010015776 Glucose oxidase Proteins 0.000 abstract description 9
- 239000004366 Glucose oxidase Substances 0.000 abstract description 9
- 235000019420 glucose oxidase Nutrition 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 9
- 239000007791 liquid phase Substances 0.000 abstract description 8
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 229940088598 enzyme Drugs 0.000 abstract description 6
- 239000012535 impurity Substances 0.000 abstract description 6
- 239000002904 solvent Substances 0.000 abstract 2
- 239000012528 membrane Substances 0.000 description 13
- 239000002356 single layer Substances 0.000 description 12
- 102000016938 Catalase Human genes 0.000 description 11
- 108010053835 Catalase Proteins 0.000 description 11
- 239000010410 layer Substances 0.000 description 5
- 239000002184 metal Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000001186 cumulative effect Effects 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 101710085938 Matrix protein Proteins 0.000 description 2
- 101710127721 Membrane protein Proteins 0.000 description 2
- 230000010718 Oxidation Activity Effects 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M4/00—Electrodes
- H01M4/86—Inert electrodes with catalytic activity, e.g. for fuel cells
- H01M4/90—Selection of catalytic material
- H01M4/9008—Organic or organo-metallic compounds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Abstract
Description
【発明の詳細な説明】
〔概要〕
ラングミュア−プロジェット法にもとづくタンパク質累
積膜の製造方法の改良である。DETAILED DESCRIPTION OF THE INVENTION [Summary] This is an improvement of a method for producing a protein accumulation film based on the Langmuir-Prodgett method.
ラングミュア−プロジェット法にもとづくタンパク質累
積膜の製造方法の液体相としてグリセリンやＮ、Ｎ−ジ
メチルホルムアミドや７セトン等の有機溶媒を使用する
ことにより、不純物を含まず高純度であり、分子レベル
で高度の配列秩序を有し、高密度のタンパク質素ｔａｇ
を製造する方法である。By using organic solvents such as glycerin, N,N-dimethylformamide, and 7setone as the liquid phase of the protein accumulation film production method based on the Langmuir-Prodgett method, it is highly pure and does not contain impurities, and can be processed at the molecular level. Protein element tag with high degree of sequence order and high density
This is a method of manufacturing.
本発明は、バイオセンサの活性要素である酵素膜（例え
ば電極等の支持体上に固定されている酵素のｌｌ５Ｉ）
等のタンパク買累積膜の製造方法に関する。The present invention relates to an enzyme membrane (for example, an enzyme 11I immobilized on a support such as an electrode) that is an active element of a biosensor.
The present invention relates to a method for producing a protein-purchasing membrane.
バイオセンサの活性要素である酵素膜は、金属性電極等
の上に酵素が膜状に固定されたものであるが、この酵素
膜等のタンパク賀累積膜を製造するために、ラングミュ
ア−プロジェット法を使用することが検討されている。Enzyme membranes, which are the active elements of biosensors, are made by fixing enzymes in the form of a membrane on metal electrodes, etc. In order to manufacture proteinaceous accumulation membranes such as enzyme membranes, Langmuir-Projet The use of the law is being considered.
その代表的な２例を示す。Two typical examples are shown below.
第１の例においては、飽和硫酸アンモニュウム溶液上に
タンパク質単分子膜を浮遊させておき、この硫醜アンモ
ニュウム溶液面を、お−むね垂直に貫通するように、金
属板・ガラス板等を移動させて、金属板・ガラス板等の
上に、タンパク質単分子膜を転写するものである。In the first example, a protein monomolecular film is suspended on a saturated ammonium sulfate solution, and a metal plate, glass plate, etc. is moved so as to penetrate the surface of the sulfuric ammonium solution almost perpendicularly. In this method, a protein monomolecular film is transferred onto a metal plate, glass plate, etc.
第２の例においては、水面上に脂肪酸単分子膜とタンパ
ク質単分子膜との結合２層体を浮遊させておき、この２
層体を、お−むね垂直に貫通するように、金属板・ガラ
ス板等を移動させて、金属板・ガラス板等の上に、上記
の２層体を転写するものである。In the second example, a bonded bilayer consisting of a fatty acid monolayer and a protein monolayer is suspended on the water surface, and
The two-layer structure is transferred onto the metal plate, glass plate, etc. by moving the metal plate, glass plate, etc. so as to penetrate the layer body approximately perpendicularly.
上記第１の例においては、製造されたタンパク質素Ｍ膜
上に硫酸アンモニュウムが析出し、その上に第２、ｔｌ
ＩＩ３のタンパク質単分子膜を累積することが困難であ
るという欠点があり、上記第２の例においては、脂肪酸
単分子膜とタンパク買単分子膜との結合２層体が累積さ
れるのであって。In the first example, ammonium sulfate is deposited on the produced protein M film, and the second, tl
There is a drawback that it is difficult to accumulate a protein monolayer in II3, and in the second example above, a bonded bilayer of a fatty acid monolayer and a protein monolayer is accumulated. .
タンパク賀単分子膜のみの累積膜が製造されるのではな
い。A cumulative film of only protein monolayers is not produced.
本発明の目的は、この欠点を解消することにあり、不純
物を含まず高純度であり、分子レベルで高度の配列秩序
を有し、高密度のタンパク賀累植膜を製造する方法を提
供することにある。The purpose of the present invention is to eliminate this drawback, and to provide a method for producing a protein graft membrane that is highly pure, does not contain impurities, has a high degree of sequence order at the molecular level, and has a high density. There is a particular thing.
上記の目的を達成するために本発明が採った手段は、ラ
ングミュア−プロジェット法にもとづくタンパク質素Ｍ
ｌ！５１の製造方法において、液体相としてグリセリン
やＮ、Ｎ−ジメチルホルムアミドやアセトン等の有機溶
媒を使用することにある。The means taken by the present invention to achieve the above object are based on the protein protein M based on the Langmuir-Prodgett method.
l! In the manufacturing method of No. 51, an organic solvent such as glycerin, N,N-dimethylformamide, or acetone is used as the liquid phase.
有機溶媒液よりなる液体相上に浮遊するタンパク賀単分
子膜に架橋剤を注入・混合すると。When a crosslinking agent is injected and mixed into a protein monolayer suspended on a liquid phase made of an organic solvent solution.
製造されるタンパク質累積膜の機械的強度が向上する。The mechanical strength of the produced protein accumulation membrane is improved.
本発明の液体相は有機溶媒であるから、上記第１例、第
２例の場合のように、タンパク質単分子膜に付着して残
留することはなく、不純物を含まず高純度であり、分子
レベルで高度の配列秩序を有し、高密度のタンパク質累
積膜を製造することができる。Since the liquid phase of the present invention is an organic solvent, it does not adhere to and remain on the protein monolayer as in the first and second examples, and is highly pure and does not contain impurities. It is possible to fabricate dense protein accumulation films with a high degree of sequence order.
以下、図面を参照しつ覧、本発明の一実施例に係るタン
パク質累積膜の製造方法について、さらに説明する。Hereinafter, with reference to the drawings, a method for producing a protein accumulation membrane according to an embodiment of the present invention will be further described.
１土１ 第１ａ図参照 水槽ｌ中に、グリセリン２を満たす。1 soil 1 See Figure 1a. Fill the water tank 1 with glycerin 2.
ＰＨ６のリン酸緩衝液に溶解したグルコースオキシダー
ゼ１．４３Ｘ １０”’Ｍを滴下した後、常温で約５分
間静置して、グルコースオキシダーゼをグリセリン２の
液面上に展開する。After dropping 1.43×10''M of glucose oxidase dissolved in a PH6 phosphate buffer, the solution is allowed to stand at room temperature for about 5 minutes to develop glucose oxidase on the surface of the glycerin 2 solution.
枠３を、グリセリンｚ上に浮かべて、グルコースオキシ
ダーゼの表面積を減少し、約３０ｄｙｎｅ／ａｍの圧力
を水平方向に印加して、グルコースオキシダーゼの単分
子８４を浮遊させる。Frame 3 is floated on glycerin z to reduce the surface area of glucose oxidase, and a pressure of about 30 dyne/am is applied horizontally to suspend single molecules 84 of glucose oxidase.
基板５を図示するように、垂直に液面を横切るように、
静かに引き上げると、グルコースオキシダーゼ単分子＠
４が基板５上に転写される。As shown in the figure, the substrate 5 is placed vertically across the liquid surface.
When gently pulled up, a single molecule of glucose oxidase @
4 is transferred onto the substrate 5.
基板５を複数回上下させればグルコースオキシダーゼ単
分子膜４は１層づつ累積する。By moving the substrate 5 up and down several times, the glucose oxidase monomolecular film 4 is accumulated one layer at a time.
以上の工程をもって製造したグルコースオキシダーゼ累
積膜のグルコース酸化活性は、溶液状態の場合よりは小
さいが、十分実用に供しうる程度の大きさを有し、しか
も、そのグルコース酸化活性は長期間安定に保持される
ことが確認された。Although the glucose oxidation activity of the glucose oxidase cumulative membrane produced through the above steps is smaller than that in the solution state, it is large enough to be used for practical purposes, and the glucose oxidation activity is maintained stably for a long period of time. It was confirmed that
追」Ｌ例
第１ｂ図参照
水槽ｌ中に、グリセリンとアセトンとのｌ：１の混合液
６を満たす、ここで、アセトンを混合した理由は、液相
の粘度を低下し、累積をより容易にするためである。Addition: Example L See Figure 1b Fill the water tank l with a l:1 mixture 6 of glycerin and acetone.The reason for mixing acetone here is to lower the viscosity of the liquid phase and make it easier to accumulate. This is for the purpose of
ｐＨ７，０のリン酸緩衝液に溶解したカタラーゼ１．４
３Ｘ １０−８Ｍを滴下した後、常温で約５分間静置し
て、カタラーゼをグリセリン・７セトン混合液６の液面
上に展開する。Catalase 1.4 dissolved in phosphate buffer pH 7.0
After dropping 3X 10-8M, the solution is allowed to stand at room temperature for about 5 minutes to develop catalase on the surface of the glycerin/7cetone mixture 6.
枠３を、グリセリン・アセトン混合液６上に浮かべて、
カタラーゼの表面積を減少し、約３０ｄｙｎｅ／Ｃｓの
圧力を水平方向に印加して、カタラーゼの単分子１１９
７を浮遊させる。Floating the frame 3 on the glycerin/acetone mixture 6,
By reducing the surface area of catalase and applying a pressure of approximately 30 dyne/Cs in the horizontal direction, single molecules of catalase 119
7 to float.
グリセリン・アセトン混合液６の濃度が約２．５％にな
るように、ゲルタール・アルデヒドを、下方から流し込
む、ゲルタール・アルデヒドの架橋効果により、カタラ
ーゼ分子は相互に結合され、カタラーゼ単分子膜７の結
合状態は安定し、機械的に強固になる。Geltal aldehyde is poured from below so that the concentration of the glycerin/acetone mixture 6 is approximately 2.5%. Due to the crosslinking effect of the geltal aldehyde, the catalase molecules are bonded to each other, and the catalase monolayer 7 is bonded to the catalase molecules. The bonded state becomes stable and mechanically strong.
基板５を図示するように、垂直に液面を横切るように、
静かに引き上げると、カタラーゼ単分子膜７が基板５上
に転写される。As shown in the figure, the substrate 5 is placed vertically across the liquid surface.
When gently pulled up, the catalase monolayer 7 is transferred onto the substrate 5.
基板５を複数回上下させればカタラーゼ単分子膜７は１
層づつ累積する。If the substrate 5 is moved up and down several times, the catalase monolayer 7 becomes 1
Accumulate layer by layer.
以上の工程をもって製造したカタラーゼ累積膜の過酸化
水素分解活性は、溶液状態の場合よりは小さいが、十分
実用に供しうる程度の大きさを有し、しかも、その過酸
化水素分解活性は長期間安定に保持されることが確認さ
れた。Although the hydrogen peroxide decomposition activity of the catalase cumulative membrane produced through the above process is smaller than that in the solution state, it is still large enough to be used for practical purposes. It was confirmed that it was maintained stably.
〔発明の効果ρ
以上説明せるとおり、本発明に係るラングミュア−プロ
ジェット法にもとづくタンパク質累積膜の製造方法にお
いては、液体相としてグリセリンφＮ、Ｎ−ジメチルホ
ルムアミド・アセトン等の有機溶媒を使用することとさ
れているので、不純物を含まず高純度であり、分子レベ
ルで高度の配列秩序を有し、高密度のタンパク質素ｉ膜
を製造することができる。[Effect of the invention ρ As explained above, in the method for producing a protein accumulation film based on the Langmuir-Prodgett method according to the present invention, an organic solvent such as glycerin φN, N-dimethylformamide/acetone, etc. is used as the liquid phase. Therefore, it is possible to produce a high-density protein film that is highly pure without containing impurities, has a high degree of sequence order at the molecular level, and has a high density.
第１ａ図は１本発明の第１の実施例に係るタンパク質素
ｍ膜の製造方法の説明図である。
第１ｂ図は１本発明の第２の実施例に係るタンパク質素
ｍ膜の製造方法の説明図である。
１００．水槽、 ２００．グリセリン、３・・・枠、
４・争・グルコースオキシダーゼの単分子膜、 ５Φ
・・基板、 ６・・φグリセリンΦアセトン混合液、
７・・・カタラーゼの単分子膜。
本発明
第１ａ図
本発明
第１ｂ図FIG. 1a is an explanatory diagram of a method for manufacturing a protein membrane according to a first embodiment of the present invention. FIG. 1b is an explanatory diagram of a method for manufacturing a protein membrane according to a second embodiment of the present invention. 100. Water tank, 200. Glycerin, 3...frame,
4. Conflict: Monolayer of glucose oxidase, 5Φ
...Substrate, 6..φglycerinφacetone mixture,
7... Monolayer of catalase. Figure 1a of the present invention Figure 1b of the present invention
Claims (1)
下して該液面上に前記タンパク質の単分子膜を浮遊させ
、 その上にタンパク質累積膜が製造される基板を前記タン
パク質の単分子膜に接触させて、前記タンパク質の単分
子膜を前記基板に転写してなすことを特徴とするタンパ
ク質累積膜の製造方法。 ［２］前記単分子膜製造後に、前記有機溶媒液中に、架
橋剤を注入、混合することを特徴とする特許請求の範囲
第１項記載のタンパク質累積膜の製造方法。[Claims] [1] Liquid protein is dropped onto the surface of an organic solvent solution to suspend a monomolecular film of the protein on the surface of the liquid, and a protein accumulation film is produced on top of the monomolecular film of the protein. 1. A method for producing a protein accumulation film, comprising: bringing a substrate into contact with the monomolecular film of the protein, and transferring the monomolecular film of the protein onto the substrate. [2] The method for producing a protein accumulation film according to claim 1, wherein a crosslinking agent is injected and mixed into the organic solvent solution after producing the monomolecular film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61025008A JPS62183882A (en) | 1986-02-07 | 1986-02-07 | Preparation of protein built-up film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61025008A JPS62183882A (en) | 1986-02-07 | 1986-02-07 | Preparation of protein built-up film |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62183882A true JPS62183882A (en) | 1987-08-12 |
Family
ID=12153904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61025008A Pending JPS62183882A (en) | 1986-02-07 | 1986-02-07 | Preparation of protein built-up film |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62183882A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1186659A1 (en) * | 2000-03-16 | 2002-03-13 | Matsushita Electric Industrial Co., Ltd. | Nucleotide detector, process for producing the same and process for forming fine particle membrane |
| JP2005535095A (en) * | 2002-08-06 | 2005-11-17 | ソントル ナショナル ド ラ ルシェルシュ ションティフィーク | Fuel cell using biofilm as catalyst for air electrode reaction and / or fuel electrode reaction |
-
1986
- 1986-02-07 JP JP61025008A patent/JPS62183882A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1186659A1 (en) * | 2000-03-16 | 2002-03-13 | Matsushita Electric Industrial Co., Ltd. | Nucleotide detector, process for producing the same and process for forming fine particle membrane |
| EP1186659A4 (en) * | 2000-03-16 | 2002-07-17 | Matsushita Electric Ind Co Ltd | Nucleotide detector, process for producing the same and process for forming fine particle membrane |
| US7129047B2 (en) | 2000-03-16 | 2006-10-31 | Matsushita Electric Industrial Co., Ltd. | Method for manufacturing a nucleotide detector |
| JP2005535095A (en) * | 2002-08-06 | 2005-11-17 | ソントル ナショナル ド ラ ルシェルシュ ションティフィーク | Fuel cell using biofilm as catalyst for air electrode reaction and / or fuel electrode reaction |
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