JPS62179392A - Production of natural dyestuff by safflower tissue culture method - Google Patents
Production of natural dyestuff by safflower tissue culture methodInfo
- Publication number
- JPS62179392A JPS62179392A JP1919186A JP1919186A JPS62179392A JP S62179392 A JPS62179392 A JP S62179392A JP 1919186 A JP1919186 A JP 1919186A JP 1919186 A JP1919186 A JP 1919186A JP S62179392 A JPS62179392 A JP S62179392A
- Authority
- JP
- Japan
- Prior art keywords
- plant
- callus
- safflower
- medium
- red
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000020518 Carthamus tinctorius Species 0.000 title claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 title claims description 9
- 235000003255 Carthamus tinctorius Nutrition 0.000 title abstract description 5
- 239000000975 dye Substances 0.000 title abstract 5
- 238000012136 culture method Methods 0.000 title 1
- 235000003642 hunger Nutrition 0.000 claims abstract description 5
- 230000037351 starvation Effects 0.000 claims abstract description 5
- 241000208838 Asteraceae Species 0.000 claims abstract 3
- 230000000644 propagated effect Effects 0.000 claims abstract 2
- 239000000049 pigment Substances 0.000 claims description 24
- 238000012258 culturing Methods 0.000 claims description 2
- 230000001902 propagating effect Effects 0.000 claims 1
- 206010020649 Hyperkeratosis Diseases 0.000 abstract description 15
- 241000196324 Embryophyta Species 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 abstract description 4
- 239000007787 solid Substances 0.000 abstract description 3
- 239000003375 plant hormone Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 13
- 230000006698 induction Effects 0.000 description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 235000020197 coconut milk Nutrition 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 241000951471 Citrus junos Species 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 244000181025 Rosa gallica Species 0.000 description 1
- 235000000533 Rosa gallica Nutrition 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000004457 myocytus nodalis Anatomy 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229940119485 safflower extract Drugs 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Abstract
Description
【発明の詳細な説明】
（Ａ） 産業上の利用分野
本発明は植物組織培養法による天然色素、特に赤紅色天
然色素の製造に関するものである。DETAILED DESCRIPTION OF THE INVENTION (A) Field of Industrial Application The present invention relates to the production of natural pigments, particularly red-red natural pigments, by a plant tissue culture method.
（Ｂ） 従来の技術
ベニ花由来の赤紅色色素は古来から化粧品、染料食品等
の着色に用いられている高価な色素であるが、従来この
色素は栽培したベニ花植物の花弁を採取し、その花弁か
ら抽出する方法によって製造されている。(B) Conventional technology The reddish pigment derived from the red rose flower is an expensive pigment that has been used since ancient times to color cosmetics, dye foods, etc., but conventionally, this pigment was obtained by collecting the petals of the cultivated red flower plant. It is produced by extracting it from the petals.
（Ｃ） 発明が解決しようとする問題点したがって、
この方法ではベニ花の栽培に広大な土地と数ケ月の時間
を要し、さらに１製造されるベニ花赤紅色色素の量・質
共に天然自然の条件に左右されるという制約があった。(C) The problem that the invention seeks to solve. Therefore,
This method requires a vast area of land and several months of time to cultivate safflower, and is also constrained by the fact that both the quantity and quality of the red crimson pigment produced are dependent on natural conditions.
（功 問題を解決するための手段
本発明者はベニ花植物の組織培養に関し、種々検討を重
ねた結果人工的に管理された一定条件下で極めて短期間
に大量のＰｊ′Ｊｄな赤紅色の天然色素の製造方法を発
明１／、従来の天然色素の具造における種々の間琶点を
解決することができた。(Means for Solving the Problem) The present inventor has conducted various studies regarding the tissue culture of safflower plants, and as a result, has produced a large amount of Pj'Jd reddish pigments in an extremely short period of time under certain artificially controlled conditions. Invention 1/ of a method for producing natural pigments has solved various problems in the conventional formulation of natural pigments.
すなはち、本発明はベニ花の種子や植物体の一部よシ植
物ホルモンを含有する培地でカルスを誘導せしめ、誘導
されたカルスを液体またけ固体培地で継代培養を行い増
殖した細胞塊を採取後ｙｔｆｉ状態に砧−＜Ｃとによ＃
）赤紅色色素を産生せしめることを特徴と一部る天然色
素の製造方法に関するものである。In other words, the present invention involves inducing callus in a medium containing phytohormones from seeds and parts of the plant, and subculturing the induced callus in a liquid and solid medium to proliferate the cells. After collecting the lump, it will be in ytfi state.
) This relates to a method for producing a natural pigment, which is characterized in part by producing a reddish pigment.
（イ） カルスの誘導及び継代培養
カルス誘導用に供されるベニ花植物の組織としては天然
へ一花／（キク、’ｆ−Ａ・ｔｈａｍｕ・八
△
ｔｉｎｃｔｏｒｉｕｓ Ｌ、 ）の種子または組織が供
され、％に幼苗の組織が望ましい。このベニ花植物の幼
苗は該植物の種子から常法に従い無菌的に発芽育成した
ものが良く、数αに育成した幼植物の根、胚軸及び子葉
がカルス誘導用のベニ花組織として供される。(b) Callus induction and subculture The tissues of the safflower plant used for callus induction include natural chrysanthemums, 'f-A.
Seeds or tissues of Δtinctorius L.) are provided, and tissues of young seedlings are preferred. The seedlings of this safflower plant are preferably those that have been germinated and grown aseptically from the seeds of the plant according to a conventional method, and the roots, hypocotyls and cotyledons of the seedlings, which have been grown to several α, are used as the safflower tissue for callus induction. Ru.
カルス誘導用及び継代培養用培地は特に限定するもので
はないが、表１に示すような公知のホワイト（ｗｈｉｔ
ｅ １９４３ ）、ムラシゲ・スクーグ（Ｍｕｒａｓｈ
ｉｇｅ、Ｓｋｏｏｇｌ ９６２ ） 、Ｂ ５ （Ｇ
ａｍｂｏｒｇ １９６８’）などの培酢＃（工ＡＡ）−
を用い、サイトカイニンとしてベンジルアデニン（ＢＡ
）またはカイ−１アー、 。The medium for callus induction and subculture is not particularly limited, but may be the known white medium as shown in Table 1.
e 1943), Murashige Skoog
ige, Skoogl 962), B5 (G
amborg 1968') etc.
benzyladenine (BA) as the cytokinin.
) or chi-1a, .
ネチンまたは２．イソペンアニへ１ノゾン（２ｊＰ
）を各々０．１〜３ ＰＰＭ添加した球天培地が良い。Netin or 2. Isopenani to 1 noson (2jP
) is recommended.
カルスの生胃促進を目的としてカゼイン加水分解物（カ
ザミノ酸）、ココナツミルクなどを用いる場合もある。In some cases, casein hydrolyzate (casamino acid), coconut milk, etc. are used to promote the growth of callus.
増殖液体培養では上記の培地から寒天をはぶいた培地に
よって好気的条件のもとに培養が行なわれる。In the propagation liquid culture, culture is carried out under aerobic conditions using the above-mentioned medium covered with agar.
培％にあたっては培養温度ｍ −Ｊ−０’Ｃより好まし
くはＷＣ前後の一定温度に保ち培養を竹う。カルス誘導
に必要な期間は３〜５週間Ｉ
であり、誘導されたカルスは固足培地上では３〜５ｉ問
おきに液体培地中では２〜４週間シ・きに新しい培地に
継代培養される。Regarding the culture percentage, the culture is maintained at a constant temperature of m-J-0'C, preferably around WC. The period required for callus induction is 3 to 5 weeks, and the induced callus is subcultured into a new medium every 3 to 5 days on a solid foot medium and every 2 to 4 weeks in a liquid medium. Ru.
（ロ）赤紅色色素の産生
カルスが十分に増殖したのち、この細＋ｒｔｉｌ塊を飢
餓条件下におき、赤紅色色素を産生せしめる。飢餓条件
とは必要な栄養源の全部または一部を欠しまたり、増殖
を阻害する条件を設定するなど、細胞に増殖をさまたげ
るようなすべての条件下をいい特にこれを限定するもの
ではない。たとえば
（１）細胞塊を培地から取り出（２，て放置する。(b) Production of reddish pigment After the callus has sufficiently grown, the fine + rtil mass is placed under starvation conditions to produce reddish pigment. Starvation conditions refer to all conditions that hinder cell proliferation, such as lacking all or part of a necessary nutrient source or setting conditions that inhibit proliferation, and are not particularly limited thereto. For example, (1) the cell mass is removed from the medium (2) and left alone.
（１１） シェフローズのみから成る培地で培養する
。(11) Cultivate in a medium consisting only of chef rose.
（吟 チッソ、リン酸、各糧ビタミンを単独る。(Gin: Chisso, phosphoric acid, and each food vitamin alone.
ｔた、上記（１）〜（ｌｉｔ）の条件は組み合わせて用
いろ事もでき、組み合わせる事によシ、さらに効率よく
赤紅色色素を得ゐ挙もできる。In addition, the conditions (1) to (lit) above can be used in combination, and by combining them, a reddish pigment can be obtained even more efficiently.
参考例Ｉ
カルスの誘導
ベニ花の種子を流水中で１時間洗浄する。次いでＩＯ係
エタノール中に瞬時浸漬し、更に１幅次亜塩素酸ナトリ
ウム溶液中にＸ分間浸漬した後、滅菌水で３回洗浄する
。殺菌処理を施した種子を寒天（含有ｌチ）上Ｋｔｇｌ
ｔ床すると約１０日間で柚子が発芽し５．３〜５３の無
菌幼苗が得らｎる。幼浦を％菌的に子葉胚軸、幼根の小
片に切断し、Ｎ Ａ Ａ （１ｐｐｍ ）、ベンジルア
デニン（１ｐｐｍ）を冨むムラシゲ・スクーグ培地（寒
天富有１幅）に各小片を１ケずつ置床し、暗黒下、８℃
で培養を行う。培養４週間後にカルス細胞が形成される
。Reference Example I: Induction of callus Seeds of safflower are washed in running water for 1 hour. Next, it is momentarily immersed in IO ethanol, further immersed in a sodium hypochlorite solution for X minutes, and then washed three times with sterile water. The sterilized seeds were placed on agar (containing 1) Ktgl.
When planted in T-bed, the yuzu will germinate in about 10 days and 5.3 to 53 sterile seedlings will be obtained. Cut the Youra into small pieces of cotyledon hypocotyl and radicle using microorganisms, and place each piece into Murashige-Skoog medium (Agar Fuyu 1 width) containing NAA (1 ppm) and benzyladenine (1 ppm). Place them on the floor in the dark at 8℃.
Culture is carried out in Callus cells are formed after 4 weeks of culture.
ｐｐｍ ）ベンジルアデニン（ｏ、 ｓ ｐｐｍ ）を
含むムラシゲ・スクーグ培地に３週間毎５〜６回継代培
養し、とのカルス（生ｉ量ｔｔｒ）１）２．４−Ｄ （
０，１ｐｍｍ ）ベンジルアデニン（０，５ｐｍｍ ）
ココナツミルク（５俤）を含むΔ−のムラシゲ・スクー
グの液体培地中で懸濁培養するとｌｔ日日後は７．４
ｐ （生型ｆ）の懸濁細胞が得られた。ppm) Calli (viable amount ttr) 1) 2.4-D (
0.1 pmm) Benzyl Adenine (0.5 pmm)
When cultured in suspension in Δ- Murashige-Skoog liquid medium containing coconut milk (5 yen), after lt days 7.4
p (live type f) suspension cells were obtained.
赤紅色色素の産生
実施例Ｉ
参考例Ｉで得られたカルスを培地から無菌的に取り出し
、殺菌済の湿りたＦ紙上に置き、乾燥しないように密閉
して２日間室＠に放置するとカルスが赤紅色となシ、ｐ
紙が赤紅色に染まった。Production of reddish pigment Example I The callus obtained in Reference Example I was aseptically removed from the culture medium, placed on sterilized damp F paper, sealed to prevent it from drying, and left in a room for 2 days. red and red, p
The paper was stained red.
実施例■
参考例■で得られた細胞を培地から無直的に取り出し、
殺醒済の湿った戸紙上装置き、乾燥しないように密閉し
て２日間室温に放置す２細胞が赤紅色となり、Ｐ電が赤
七［色に染まった。Example ■ The cells obtained in Reference Example ■ were taken out directly from the culture medium,
Place the device on the damp paper that has been sterilized, seal it tightly to prevent it from drying out, and leave it at room temperature for 2 days.The two cells turn reddish and the P cells are stained red.
ｅｓ、施例ｍ
参考例■で得られた細胞を’ｉｆ地から無菌的にとり出
ｔ／、シークロース（Ｓ−Ｏい）のみから成る培地で２
日間培養すると岬！泡が赤紅色となった。es, Example M The cells obtained in Reference Example
After culturing for days, it becomes a cape! The bubbles turned red.
実施例■
実施ＦＪ １１で得られたｒＩ胞を無菌的に取り出し、
殺菌済の湿ったｐ紙上に置き、乾燥しないように密閉し
て２日間室温に放置すると細胞がさらに赤紅色となり、
ｐ紙が赤紅色に染まった。Example ■ The rI vesicles obtained in Implementation FJ 11 were aseptically removed,
When placed on sterilized damp P paper, sealed tightly to prevent drying, and left at room temperature for 2 days, the cells become more reddish.
The paper was dyed red.
実施例Ｖ
参考例１．【で得られた紬ｉ１地から無菌的にとり出し
１、表２で示す貧栄養培地でそれぞれ２週間培養すると
細胞は赤紅色となった。さらに、この細胞を無菌的に取
シ出し、殺菌済の湿った戸紙上Ｋｍき、乾燥しないよう
に密閉して２日間室温に放置すると細胞はさらに赤紅色
となり、Ｆ紙が赤紅色に染まりた。Example V Reference Example 1. The cells were aseptically removed from the pongee fabric obtained in [1] and cultured in the oligotrophic medium shown in Table 2 for 2 weeks, and the cells became reddish in color. Furthermore, when these cells were removed aseptically, placed on a sterilized damp paper, sealed tightly to prevent drying, and left at room temperature for 2 days, the cells became even more reddish, and the F paper was stained reddish. .
０う 赤紅色色素の製造
赤紅色色素を産生じた細胞及び培養液から赤紅色色素を
製造するには、ベニ花生４Ｅ、よシ赤紅色色素を製造す
る場合と同様な抽出法、あるいは含水アセトン等による
抽出を行う。これをアセトン♂Ｏ％水溶液に転浴して吸
収極大（λｍａｘ ） を測定したところλｍａｘ＝
５Ｉ５ｎｍ
であった。また本色素はベニ花生花抽出色素と同様な物
性を示し、水浴液ｐＨを塩基性域にすれば溶解するが、
酸性域では示紅色の沈澱を生じる。0 Manufacture of reddish pigment To manufacture reddish pigment from the cells and culture solution that produced reddish pigment, extraction methods similar to those used for producing reddish pigment, such as Benihana 4E and Yoshii, or aqueous acetone can be used. Extract by etc. When this was transferred to an acetone♂O% aqueous solution and the absorption maximum (λmax) was measured, λmax=
5I5nm. In addition, this pigment shows the same physical properties as the safflower extract pigment, and dissolves if the pH of the water bath solution is made into a basic range.
In acidic areas, a reddish precipitate is formed.
（Ｋ） 発明の効果゛
上述のごとく本発明方法によれば、品質が一定で天然品
と変わらぬ赤紅色色素が季節、天候など自然条件に左右
されず、また植物栽培のための広大な土地を必要とせず
、しかも短期間に効率よく製造される。(K) Effects of the Invention As mentioned above, according to the method of the present invention, a reddish pigment of constant quality and same as that of natural products can be produced without being affected by natural conditions such as season and weather, and a large area of land for plant cultivation can be obtained. It can be manufactured efficiently and in a short period of time without the need for
ン、ワイン等の着色剤として使用できるばかりでなく、
その′１ま、またはレーキとして香粧品原料や染料とし
て広く使用することができる。Not only can it be used as a coloring agent for wine, wine, etc.
It can be widely used as a raw material for cosmetics or as a dye or as a lake.
ｉ ｚ 工 賢 艷 艷 ま 鉗 古
○ ＾
５ ｚ ５ 旨 ｉ ｉ 遊 占 ＝ ６１Ｃｏａｌ、
１６ＨｚＯ
Ｔｈｉａｍｉｎｅ−ＨＣＩ
Ｐｙｒｉｄｏ−ｘｉｍｅ−ＨＣＩ
ＮｉｃｏｔｉｒＩＬｃ ａｃｉｄ
Ｇｔｌｙｃｉｎｅ
ｉｎ０８ｉｔｏｌ ’５ｕｃｒｉ、ｓ
ｅ ３０（２，４−Ｄ
Ｂ、Ａ
″吋ツミルク ５．）。i z 工 連 艷 艷 ま 鉗 古○ ＾ 5 z 5 い い 連 連 = 61Coal,
16HzO Thiamine-HCI Pyrido-xime-HCI NicotirILc acid Glycine in08itol '5ucri,s
e 30 (2,4-D B, A ″ thick milk 5.).
ｉ地Ａ 培地Ｂ 培地Ｃ培地Ｄ
コ（５／リ （ｍｇ／リ （ｍｉ’） （ｍｇ
／す０．０２５ ０ ０．（
’＞７５ ００．１ ０ ０．１
０
０．５ ０ ：）、５ ００．５ ０
Ｑ、５ ０
Ｔ、ＯＯ２，Ｏ０
Ｌ００ ０ １００ ０
０，５０，５００
以 上i soil A medium B medium C medium D (5/li (mg/li (mi') (mg
/s0.025 0 0. (
'>75 00.1 0 0.1
0 0.5 0 :), 5 00.5 0
Q, 5 0 T, OO2, O0 L00 0 100 0 0,50,500 or more
Claims (1)
養により増殖せしめた後、該増殖した細胞を１回または
２回以上飢餓条件下におくことにより赤紅色色素を産生
せしめる事を特徴とする天然色素の製造方法It is characterized by producing a reddish pigment by culturing and propagating cells induced and propagated from a safflower plant belonging to the Asteraceae family, and then subjecting the proliferated cells to starvation conditions once or twice or more. Natural pigment manufacturing method
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1919186A JPS62179392A (en) | 1986-02-01 | 1986-02-01 | Production of natural dyestuff by safflower tissue culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1919186A JPS62179392A (en) | 1986-02-01 | 1986-02-01 | Production of natural dyestuff by safflower tissue culture method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62179392A true JPS62179392A (en) | 1987-08-06 |
Family
ID=11992449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1919186A Pending JPS62179392A (en) | 1986-02-01 | 1986-02-01 | Production of natural dyestuff by safflower tissue culture method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62179392A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62257390A (en) * | 1986-04-30 | 1987-11-09 | Kibun Kk | Production of yellow dyestuff of safflower |
| JPS6447387A (en) * | 1987-08-18 | 1989-02-21 | Kibun Kk | Production of red pigment of safflower |
| JPH02276580A (en) * | 1989-04-19 | 1990-11-13 | Mitsui Eng & Shipbuild Co Ltd | Production of red coloring matter from cultured safflower cell |
-
1986
- 1986-02-01 JP JP1919186A patent/JPS62179392A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62257390A (en) * | 1986-04-30 | 1987-11-09 | Kibun Kk | Production of yellow dyestuff of safflower |
| JPH0251598B2 (en) * | 1986-04-30 | 1990-11-07 | Kibun Kk | |
| JPS6447387A (en) * | 1987-08-18 | 1989-02-21 | Kibun Kk | Production of red pigment of safflower |
| JPH02276580A (en) * | 1989-04-19 | 1990-11-13 | Mitsui Eng & Shipbuild Co Ltd | Production of red coloring matter from cultured safflower cell |
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