JPS62122666A - Production of deodorant - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] 〔Technical field〕 The present invention relates to a method for producing a deodorant that eliminates bad odors and foreign odors.

[Background technology]

Generally, the sources of bad odors and strange odors are factory smoke and waste liquid, cigarettes, frozen fields, kitchen waste, etc., and the types of bad odors and strange odors are diverse. especially,
In homes, rooms are often kept closed when heating and cooling equipment is in operation, so countermeasures against these foul odors have become a major problem.

There are various ways to eliminate such bad odors and foreign odors, but deodorants containing plant extracts as active ingredients are attracting attention. In addition to being odorless itself,
It is safe and does not require large-scale equipment. However, the plants used as raw materials are natural and depend on the external environment, i.e. wind and rain.

Its stable supply is affected by temperature, disease, and human factors (spraying of herbicides and pesticides), and
The plant itself and the deodorizing active ingredients contained therein may be damaged.

[Purpose of the invention]

This invention was made in view of the above circumstances, and provides a method for producing a deodorant that is odorless itself and has a high deodorizing effect without being affected by the external environment. The purpose is to

[Disclosure of the invention]

In order to provide a method for producing a deodorant that does not have the above-mentioned conventional problems, the inventors have conducted various experiments. Therefore, we attempted tissue culture of a plant of the genus Forsythia, which has a particularly high deodorizing effect on sulfur-based odors.

BACKGROUND ART A method of inducing callus from embryos in seeds of shoot tips, stems, and roots of plant tissues and culturing the tissues on solid or liquid media was started in the early 1900s. up to now,
There are examples of culturing plants such as carrots, rice, orchids, and cymbidiums. Although there were limited success stories with woody plants, the inventors further considered and cultivated the tissues of Forsythia plants to obtain callus, from which they were able to obtain an effective deodorizing ingredient.In other words, this invention describes a method for producing a deodorant characterized by extracting callus cultured from a plant of the genus Forsythia to obtain an active ingredient, which will be described in detail below.

As a plant of the genus Forsythia, Forsythia
hia 5uspensa) + Yamatorengo (
Forsythia japonica) + Forsythia viridissi
ma ) + Forsyth
iakoreana) etc. can be used. Cultivation is performed using explanted leaves or stems of the target plant. The solid medium composition includes auxin of 10-4 to 1
in the range of 0-7 mol/l, and cytokinin in the range of 10-
Those containing each in the range of 4 to z+-r mol/1 are used. For example, in the synthetic medium of Linsmeyer-Skoog medium, ShotJ! 1-5% by weight (preferably about 3%
wt%), agar 0.5-1.0 wt% (preferably about 0
．． 8% by weight), and further contains α-naphthaleneacetic acid as auxin and kinetin as cytokinin in the above range.

The callus of a plant of the genus Forsythia obtained by inducing and culturing callus on such a medium is subjected to extraction to obtain a deodorizing active ingredient. When the pH of the solvent in which the obtained deodorizing component is dissolved is kept from neutral to slightly alkaline, the effect of the deodorizing active component such as the phenolic compound is further brought out.

The method for making the solution weakly alkaline rather than neutral is not particularly limited as long as it can be made weakly alkaline rather than neutral by adding the solution to the active ingredient. Na
Examples include alkaline solutions such as OH, KOH, Na2HPO4, and liquids having a buffering effect.

The higher the hydrogen ion concentration of the solution, the more effective it is at deodorizing.
Since care must be taken in handling, please use at a pH of 6.5 to 8.
About 5 is preferable.

This will be explained in more detail below using examples.

(Examples 1 to 6 and Comparative Examples) 4o1 of Linsmeyer-Skoog medium (containing 3% by weight of sucrose and 0.8% by weight of agar) was dispensed into a 100 ml Ellenmeyer flask and sterilized by a conventional method. The pH before sterilization was approximately 5.7. Into this medium, α-naphthalene acetic acid and kinetin listed in Table 1 below were added.

The petioles of Forsythia chinensis and Forsythia chinensis (
1 to 3 φXIQ+n) were sterilized by a conventional method using a 5% aqueous solution of sodium hypochlorite. These were placed on the above medium and incubated at 27°C in the light (3000j!x) for 10 hours.
Callus induction was performed under the conditions of +14 hours of darkness. After callus induction, culture was carried out every two weeks for two months. 60”c on the resulting callus.

Hot water extraction was performed for 5 hours, the extract was filtered, and the resulting filtrate was concentrated to dryness to obtain the deodorizing active ingredient as a solid. This was dissolved in water and the pH was further adjusted to 7 to obtain a 0.5% by weight deodorant solution.

In order to examine the deodorizing effect of the deodorant solution thus obtained, the following test was conducted.

200 ppm of methyl mercaptan was added to a 10 ml test tube that could be tightly stoppered, and 1 ml of each of the above six types of deodorant solutions was added and the tube was tightly stoppered. After a certain period of time had elapsed, the concentration of methyl mercaptan was measured by gas chromatography. An FPD was used as a detector.

As a blank test, the concentration of methyl mercaptan was measured in the same manner using a solution to which no deodorizing active ingredient was added. The deodorizing rate was determined according to the following formula, and the deodorizing effect was examined. The results are shown in Table 1 along with the growth status of callus.

o Xl Odor elimination rate (%) = x100O The deodorizing component was extracted from the resulting callus and the deodorizing effect was examined in the same manner.

As shown in the table, the method for producing a deodorant according to the above example yields a deodorant that exhibits good deodorizing ability, and it is possible to obtain a deodorant from components extracted from cultured plant tissues. became. However, it cannot be said that the deodorizing rate of awls is good, and there appears to be variation in the deodorizing active ingredients obtained from tissue-cultured plant cells.

〔Effect of the invention〕

Since the method for producing the deodorant according to the present invention is configured as described above, it does not cause the above-mentioned conventional problems, is not affected by the external environment, is itself odorless, and is not dangerous to handle. A deodorant with high deodorizing effect can be obtained.

Claims (6)

[Claims] (1) A method for producing a deodorant, which comprises extracting callus cultured from a plant of the genus Forsythia to obtain an active ingredient. (2) The method for producing a deodorant according to claim 1, wherein the callus is cultured from leaves or stems of plants. (3) Callus absorbs auxin by 10^-^4~10^-
range of ^7 mol/l and cytokinin in the range of 10^-
The method for producing a deodorant according to claim 1 or 2, wherein the deodorant is cultured on a solid medium containing each in the range of 4 to 10 to 6 mol/l. (4) The method for producing a deodorant according to claim 3, wherein the auxin is α-naphthaleneacetic acid. (5) The method for producing a deodorant according to claim 3, wherein the cytokinin is kinetin. (6) The method for producing a deodorant according to any one of claims 3 to 5, wherein the solid medium is a Linsmeyer-Skoog medium.