JPS62121365A - Automatic analyzing instrument - Google Patents

Abstract

PURPOSE:To prevent the deterioration of analyzing accuracy in a main photometric means by providing a specimen supplying means, reagent supplying means, main photometric means, auxiliary photometric means, etc. CONSTITUTION:The specimen supplying means 4, the reagent supplying means 5, the main photometric means 8 and the auxiliary photometric means 6, etc., are provided. An arm 24 is rotated and adjusted to position a rotary magnet 14 onto a reaction cuvette 13, then a cylindrical body 25 is inserted into the cuvette 13 by adjusting a rack pinion 23. The magnet piece 14 is detached by ejection and is introduced into the cuvette 13. The body 25 is thereafter raised from the cuvette 13 by adjusting the pinion 23 and in succession, the cuvette 13 is moved in the direction of an arrow. The specimen is dispensed into the cuvette in the means 4 and the 1st reagent is dispensed therein in the means 5. The reaction cuvette 15 is thereafter brought into a stirring part 10 where the magnet piece 14 is rotated by a rotary magnetic field generator to stir and uniformly mix the specimen and reagent. The optical density of the reaction liquid of the cuvette 15 is measured by the means 6. The 2nd reagent is dispensed into the cuvette by the 2nd reagent supplying means 7 and the similar operation is repeated in the stirring part 11 and the means 8.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application This invention relates to an automatic analyzer. More specifically, measurements during the reaction process using a plurality of reagents are performed with high precision, and the final analytical precision is improved.

Regarding automatic analysis equipment.

(b) Conventional technology Automatic analyzers that perform analysis and measurement based on optical density, which is directly measured after reaction by adding multiple reagents to a sample in a transparent reaction cuvette, have diversified analysis methods and improved analysis accuracy. Therefore, in recent years, even before the final reagent is added, optical properties of the mixed liquid in the reaction cuvette are measured once or several times during the process up to the final reaction after each reagent is added to the sample. It is increasingly being used for data processing, and in particular, a method of detecting the optical density before the final reaction as a baseline for changes in absorbance is widely used. On the other hand, from the perspective of improving analysis accuracy, it is necessary to stir the sample and each reagent to eliminate locality in the mixture in the reaction cuvette. For this reason, automatic analyzers have conventionally adopted an indirect method using an air jet or a direct method using a stirring rod as stirring means.

(c) Problems to be solved by the invention However, indirect methods using air jets etc.
In the case of a rectangular reaction cuvette, it is difficult to confirm that the stirring effect is sufficient; on the other hand, when using a direct method using a stirring rod, etc., the sample and reagent mixture adheres irregularly to the stirring rod, etc., and is carried out of the reaction cuvette. Therefore, by stirring before adding all the reagents necessary for the reaction, an error will occur in the difference in volume between the mixed liquid before the final reaction and the reaction liquid after the final reaction, leading to a decrease in analytical accuracy. There was this inconvenience.

This invention was made in view of the above circumstances, and provides an automatic analyzer that can perform efficient continuous analysis without causing a decrease in analysis accuracy due to stirring of the mixed liquid in the reaction cubera (before the final reaction). This is what we are trying to provide.

(d) Means for Solving the Problems Thus, according to the present invention, there is provided a reaction line in which the reaction cuvette is movable, a specimen supply means for supplying a specimen to the reaction cuvette on the reaction line, and a A plurality of reagent supply means are attached sequentially and supply reaction reagents to the reaction cuvettes supplied with the specimen, and a reagent supply means is attached after the plurality of reagent supply means and is attached to the optical system for controlling the mixed liquid in the reaction cuvette after the final reaction. An automatic analyzer comprising a main photometric means for measuring the concentration, and an auxiliary photometric means for measuring the optical density of the mixed liquid in the reaction cuvette before the final reaction, which is attached before the final reagent supply means. , a means for introducing the rotating magnet into the reaction cuvette before the auxiliary photometric means, and a means for discharging the rotating magnet out of the cuvette by magnetic force behind the main photometric means; There is provided an automatic analysis apparatus characterized in that a rotating magnetic field generating device capable of rotating the rotating magnet in the reaction cuvette is attached at or immediately before the photometric position of the photometric means.

The device of the present invention is particularly suitable for an automatic analyzer that uses a plurality of reagents to react with a biochemical sample such as serum, plasma, urine, etc. to develop color, and quantifies each biochemical measurement item using an optical measurement method.

The rotating magnet introduction means and the ejection means used in this invention may be independent, but they may also be structured so that they can be introduced and ejected as appropriate. As shown in FIG.
It is suitable to use a rotating magnet attachment/detachment means provided with a bar-shaped release that moves up and down within this cylindrical body, pushes out the rotating magnet when it is attracted, and can be detached from the lower end. However, other than this, it is also possible to use one that can be attached and detached as appropriate by switching the electromagnet.

The rotating magnet may be inserted into the cuvette upstream of the auxiliary photometric means, and may be either before or after the sample supply means or before or after the reagent supply means. It should be noted that a plurality of auxiliary photometering means may be set depending on the purpose, or in this case, it is necessary to set the auxiliary photometering means at least before the first auxiliary photometry means.

As the rotating magnet and the rotating magnetic field generating device used in the device of this invention, it is suitable to use a magneti soxtara which is normally used in the field, and the rotating magnet includes a permanent magnet wrapped in chemical-resistant resin. The shape of the magnet may be any of those used in the art, but a disk shape is preferred for ease of rotation within the reaction cuvette.

Further, from the viewpoint of stirring efficiency, it is preferable to have fins molded on the surface of the disc-shaped magnet.

The number of rotating magnetic field generators used and the location where the devices are installed are appropriately selected depending on the type of reagent to be added and the photometric means, etc.; Preferably just before the position.

(e) Effect In this invention, since the rotating magnet and the rotating magnetic field generating device are used as the stirring means as described above, there is no possibility of fluctuation in the size of the adult due to the reaction liquid being taken out of the cuvette before the measurement of the final reaction liquid is completed. Does not occur.

The present invention will be described in detail below with reference to Examples, but the present invention is not limited thereby.

(F) Embodiment FIG. 1 is an explanatory diagram showing the structure of an embodiment of the apparatus of the present invention and its driving state over time.

The automatic analyzer (1) in this figure is of a direct photometry type and has a two-reagent system. In the figure, (3) is the reaction line, (4
) is a sample supply unit consisting of an autosampler, (5) is a first reagent supply means (first reagent dispenser), (7) is a second reagent supply means (second reagent pressure divider), (6), (8) is a photometric means consisting of an absorption photometer, (9) is a rotating magneton introducing section, (1
0) and (11) are stirring sections, and (12) is a rotating magnet discharge section.

The reaction line (3) is configured such that a large number of reaction kiss beds (13) are intermittently moved in the direction of the arrow by a driving body (not shown) consisting of a belt conveyor.

A device shown by a rotating magnet attachment/detachment device (2) is used for introducing and discharging the rotating magnet. The device (2) includes a rack pinion for vertical movement (23) and a gear for horizontal rotation (
a vertical shaft (21) having a vertical shaft (22), an arm (24) horizontally attached to the tip of the shaft, and a fluororesin-coated iron member attached downward from the arm (24) parallel to the shaft (21). The cylindrical body (25), the cylindrical body (
It consists of a release (26) that moves up and down inside the release drive device (25) and whose tip is made of a non-magnetic material such as stainless steel, and the release (26) is connected to a release drive device (27). The device (27) causes the release (26) to move up and down inside the cylindrical body to operate the attachment and detachment of the rotating magnet (14) to and from the device (2).

As shown in Fig. 2, the rotating magnet (14) is made of fluororesin with a built-in permanent magnet, is molded into a disc shape, and has four fins (114) on its surface, while the rotating magnetic field The generator (100) is made up of a rotating magnet (101) and a motor (+02).

In the above apparatus (1), first, in the rotating magnet introduction part (9) of the reaction line (3), the rotating magnet attachment/detachment device (2) attaches magnetically to the tip of the cylindrical body (25) of the apparatus (2). Adjust the rotary magnet (J4) held by the cylinder by rotating the arm (24) so that it is positioned above the reaction cuvette (13), and then adjust the rack and pinion (23) to position the rotating magnet (J4) above the reaction cuvette (13). 25) is inserted into the reaction cuvette (13), and the release (26) is driven to extrude and detach the rotating magnet (14), which is then introduced into the cuvette (13).

Thereafter, the vaginal cylinder (25) is raised from the cuvette (13) by adjusting the rack and pinion (23),
Subsequently, the cuvette holding the rotating magnet (14) at the bottom is moved in the direction of the arrow, and the sample is dispensed into the sample supply means (4), and then the first reagent is dispensed into the first reagent supply means (5). be done. After this nuclear reaction cuvette 1-
(15) is a stirring section (10) in which a rotating magnetic field generator (100) provided at the bottom of the cuvette rotates and stirs a rotating magnet (4) held inside the cuvette to mix the sample. After uniformly mixing the reaction solution with the reagent, the optical density of the reaction solution is measured and recorded using the auxiliary photometric means (6) in the reaction cuvette. After that, the second reagent is dispensed in the second reagent supply means (7), followed by the stirring part (1
1) and the main photometry means (8), the same operations as above are repeated and recorded.

Thereafter, in the rotating magnet ejecting section (12), the arm of the rotating magnet attachment/detachment device (2) rotates, and the cylindrical body (25) is positioned above the reaction cuvette (16). By the reverse operation, the rotating magnet (14) in the cuvette is ejected.

The above series of operations is repeated to automatically analyze samples in sequence.

These operations are controlled by a control section (not shown).

(g) Effects of the Invention According to the apparatus of the present invention, changes in the reaction process at the stage before adding the final reagent can be accurately measured by stirring the reaction solution without removing it from the cuvette. It is possible to prevent deterioration in the splitting accuracy in the main photometric means. And it is possible to accurately know the information in the sample on the final reaction surface. Furthermore, the reaction solution can be uniformly stirred regardless of the shape of the cuvette.

[Brief explanation of drawings]

FIG. 1 is a configuration explanatory diagram showing an apparatus according to an embodiment of the present invention and its driving state over time, and FIG. 2 is a configuration explanatory diagram illustrating a rotating magnet and a rotating magnetic field generating device used in the apparatus of the present invention. be. (1)... Automatic analyzer of this invention, (2)...
...Rotating magnet attachment/detachment device, (3)...Reaction line, (4)...Sample supply means, (5)...
・First reagent supply means, (6)...auxiliary photometry means, (
7)...Second reagent supply means, (8)...
Main photometry means, (9)...Rotating magnet introduction section, (
10), (+1)... Stirring section, (12)...
-...Rotating magnet ejection section, (13), (15), (16
)・・・Reaction cuvette, (14)・・・Rotating magnet, (21)・・・Vertical axis, (
22)...Gear, (23)...
・Rack and pinion, (24)...Arm,
(25)...Cylindrical body, (26)...
・-・Release, (27)・・・Release drive device, (2g), (29)・・Motor, (+00)・・Rotating magnetic field generator, (101)
...-...Magnet, (102)...Motor,
(114)...Finn.

Claims (1)

[Scope of Claims] 1. A reaction line in which a reaction cuvette is movable, a specimen supply means for supplying a specimen to the reaction cuvette on the reaction line, and a reaction line to which a specimen is supplied, which is sequentially attached in the moving direction of the reaction line. a plurality of reagent supply means for supplying reaction reagents to the cuvette; a main photometry means attached after the plurality of reagent supply means for measuring the optical density of the mixed liquid in the reaction cuvette after the final reaction; An automatic analyzer comprising: an auxiliary photometric means that is attached upstream of the reagent supply means and measures the optical density of the mixed liquid in the reaction cuvette before the final reaction; means for introducing the rotating magnet into the reaction cuvette, and means for ejecting the rotating magnet out of the cuvette by magnetic force behind the main photometry means, and at or immediately before the photometry positions of the main photometry means and the auxiliary photometry means, An automatic analysis device characterized in that it is equipped with a rotating magnetic field generator capable of rotating the rotating magnet in the reaction cuvette.